An overview of cultured meat and stem cell bioprinting: How to make it, challenges and prospects, environmental effects, society's culture and the influence of religions

Journal of Agriculture and Food Research, Journal Year: 2024, Volume and Issue: 18, P. 101307 - 101307

Published: July 22, 2024

Meat is the main and important source of protein, minerals, vitamins plays a crucial role in human nutrition. Based on projections, consumption it expected to increase twofold by 2050 as result population growth. Conversely, this surge meat production gives rise issues such indiscriminate slaughter animals, escalation methane gas subsequent exacerbation global warming phenomenon, heightened risk shared diseases between humans emergence antibiotic-resistant strains pathogens, well excessive water usage. There will be significant amount, therefore, imperative explore novel approaches, employing lab-grown meat. Current research field primarily centered around cultured These meats offer numerous benefits, their eco-friendliness ability mitigate animal-borne illnesses. In process cultivating meat, stem or satellite cells are extracted from animal's muscle tissue without causing harm animal. then placed culture medium containing fetal bovine serum. Subsequently, they employ either edible non-edible scaffolds means facilitating cell proliferation, which introduced into bioreactor induce growth tissue. Under optimal conditions, including appropriate temperature, oxygen levels, nutrient availability, factors, typically takes approximately 3–5 weeks for thin piece develop. Presently, feasibility adopting severely limited its exorbitant cost, substandard colour taste, lack acceptance various religious groups. Enhancing can achieved incorporating compounds like red beetroot saffron, while taste enhanced fatty acids, fats, protein. It acknowledge that technology still initial phases order achieve widespread production, attain satisfactory level consumer approval. Furthermore, adoption may potentially lead decrease income livestock breeders. However, given inherent risks associated with industry, proceed direction. Also, 3D printing an advancing digital has vast market potential food nutrition technology. offers platform creating distinctive products have improved sensory nutritional qualities, specifically tailored particular consumer. provide distinct solutions challenges controlling fat, other composition, lifelike texture. The man-made materials used engineering polystyrene, polylactic acid (PLA), polyglycolic (PGA), mixture acids called PLGA.

Language: Английский

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3D bioprinting: Advancing the future of food production layer by layer

Food Chemistry, Journal Year: 2025, Volume and Issue: 471, P. 142828 - 142828

Published: Jan. 9, 2025

Language: Английский

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Versatile platforms of mussel-inspired agarose scaffold for cell cultured meat

Journal of Advanced Research, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Biomaterial scaffolds are critical for cell cultured meat production. polysaccharide lack essential animal adhesion receptors, leading to significant challenges in proliferation and myogenic differentiation. Thus, enhancing growth on is strongly required supply the gaps cell-cultured This study aims develop a multifunctional cell-responsive hydrogel scaffold vitro production of myofibers structured through "cell adhesion-proliferation-differentiation" strategy. A polydopamine coating was applied agarose using dipping technique. The capability myofiber preparation assessed by evaluating adhesion, proliferation, Utilizing isolated porcine skeletal muscle satellite cells (PSMSCs), feasibility pork tissue supported film further investigated three-dimensional imaging scanning electron microscopy analysis. physicochemical properties were evaluated staining texture incorporation facilitated remarkable 100 % rate scaffolds, which also demonstrated reusability. retained adequate mechanical properties, enabling adhered proliferate effectively differentiate into myofiber. Moreover, PSMSCs maintained potential thereby imparting with ability generate substantial quantities multinucleated myofibers. Furthermore, we established culture model, characterized high-density exhibited color typical real pork. innovative agarose/polydopamine functions as platform culture, offering novel avenues diversification scalable meat, promising reductions costs meat.

Language: Английский

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Cultured meat production fuelled by fermentation

Trends in Food Science & Technology, Journal Year: 2021, Volume and Issue: 120, P. 48 - 58

Published: Dec. 24, 2021

Language: Английский

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Spent media analysis suggests cultivated meat media will require species and cell type optimization

npj Science of Food, Journal Year: 2022, Volume and Issue: 6(1)

Published: Sept. 29, 2022

Cell culture media design is perhaps the most significant hurdle currently facing commercialization of cultivated meat as an alternative source dietary protein. Since optimization for a specific system requires amount effort and investment, major question remaining whether formulations can be easily shared across multiple production schemes cells different species lineages. Here, we perform spent medium analysis to compare nutrient utilization primary embryonic chicken muscle precursor fibroblasts murine C2C12 myoblast cell line. We demonstrate that these related types have significantly patterns collectively on per-cell basis, many components conventional do not appear depleted by cells. Namely, glucose was consumed rapidly nor completely precursors compared other overall, there were differences in consumption rates several key nutrients over first day culture. Ultimately, our results indicate no one likely ideal cost effective novel methods streamline efforts will important industry develop.

Language: Английский

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Harvest of quality-controlled bovine myogenic cells and biomimetic bovine muscle tissue engineering for sustainable meat production

Biomaterials, Journal Year: 2022, Volume and Issue: 287, P. 121649 - 121649

Published: June 25, 2022

Alternative technology for meat production holds the potential to alleviate ethical, environmental, and public health concerns associated with conventional production. Cultured produced using cell culture promises become a viable alternative animal-raised future of food industry. In this study, biomimetic bovine muscle tissue was artificially fabricated from myogenic cells extracted meat. Our primary method relies on three key factors; sequential digesting process, enzymatic treatment pronase, coating laminin fragment dishes. This allows efficient collection large numbers cheek meat, purifies mixture, then continuously grows in vitro. addition, our "quality control" methods, we were able determine "cell quality", including proliferative differentiation capability each step culture. Furthermore, mimic native quality-controlled cultured micropatterned thermoresponsive substrate stimulating native-like aligned structure cells, which transferred onto fibrin-based gel. gel-based environment promoted structural functional maturation resulting tissues sarcomere structures, membrane contractile ability. We believe that these features "tissue-engineered meat" are important will need nutrients, texture taste. Therefore, approach provide new platform produce more tissue-engineered near future.

Language: Английский

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Biotechnological and Technical Challenges Related to Cultured Meat Production

Applied Sciences, Journal Year: 2022, Volume and Issue: 12(13), P. 6771 - 6771

Published: July 4, 2022

The constant growth of the population has pushed researchers to find novel protein sources. A possible solution this problem been found in cellular agriculture, specifically production cultured meat. In following review, key steps for vitro meat are identified, as well most important challenges. main biological and technical approaches taken into account discussed, such choice animal, animal-free alternatives fetal bovine serum (FBS), cell biomaterial interactions, implementation scalable sustainable biofabrication culturing systems. light findings, promising is, discussed challenges an initial stage. Hence, research must overcome these ensure efficient large-scale production.

Language: Английский

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Risk assessment of cultured meat

Trends in Food Science & Technology, Journal Year: 2023, Volume and Issue: 138, P. 491 - 499

Published: July 3, 2023

Language: Английский

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Bioactive cellulose acetate nanofiber loaded with annatto support skeletal muscle cell attachment and proliferation

Frontiers in Bioengineering and Biotechnology, Journal Year: 2023, Volume and Issue: 11

Published: Feb. 23, 2023

Electrospinning emerged as a promising technique to produce scaffolds for cultivated meat in function of its simplicity, versatility, cost-effectiveness, and scalability. Cellulose acetate (CA) is biocompatible low-cost material that support cell adhesion proliferation. Here we investigated CA nanofibers, associated or not with bioactive annatto extract (CA@A), food-dye, potential muscle tissue engineering. The obtained nanofibers were evaluated concerning physicochemical, morphological, mechanical biological traits. UV-vis spectroscopy contact angle measurements confirmed the incorporation into surface wettability both scaffolds, respectively. SEM images revealed are porous, containing fibers no specific alignment. Compared pure CA@A showed increased fiber diameter (420 ± 212 nm vs. 284 130 nm). Mechanical properties induces reduction stiffness scaffold. Molecular analyses while scaffold favored C2C12 myoblast differentiation, annatto-loaded proliferative state these cells. These results suggest combination cellulose loaded may be an interesting economical alternative long-term cells culture application

Language: Английский

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Bovine muscle satellite cells in calves and cattle: A comparative study of cellular and genetic characteristics for cultivated meat production

Current Research in Food Science, Journal Year: 2023, Volume and Issue: 7, P. 100545 - 100545

Published: Jan. 1, 2023

This study compared the cellular and genetic characteristics of bovine skeletal muscle satellite cells (SMSCs) from Hanwoo (a Korean native cattle breed), including calves mature cattle. SMSCs were isolated using magnetic-activated cell sorting (MACS) tissue samples six (three three cattle) CD29 antibody. Calves' exhibited significantly faster growth rates than did those (P < 0.01), with a doubling time 2.43 days. Genetic analysis revealed higher MyoD Pax7 expression in during proliferation 0.001). However, FASN PLAG1 levels both differentiation These findings highlight need for strategies to improve produce competitive cultivated meat at price.

Language: Английский

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