International Journal of Agricultural Sustainability, Journal Year: 2025, Volume and Issue: 23(1)
Published: March 20, 2025
Language: Английский
BMC Microbiology, Journal Year: 2024, Volume and Issue: 24(1)
Published: July 9, 2024
Abstract Background Alternaria alternata is the primary pathogen of potato leaf spot disease, resulting in significant yield losses globally. Endophytic microorganism-based biological control, especially using microorganisms from host plants, has emerged as a promising and eco-friendly approach for managing plant diseases. Therefore, this study aimed to isolate, identify characterize endophytic fungi healthy leaves which had great antifungal activity A. vitro vivo. Results An fungal strain SD1-4 was isolated identified Talaromyces muroii through morphological sequencing analysis. The exhibited potent against Lill, with hyphal inhibition rate 69.19%. Microscopic scanning electron microscope observations revealed that grew parallel to, coiled around, shrunk deformed mycelia Lill. Additionally, enzyme activities chitinase β-1, 3-glucanase significantly increased hyphae Lill when co-cultured SD1-4, indicating severe impairment cell wall function Furthermore, mycelial growth conidial germination were suppressed by aseptic filtrate rates 79.00% 80.67%, respectively. Decrease disease index 78.36 37.03 also observed plants treated along characters including height, root length, fresh weight, dry chlorophyll content photosynthetic seedlings. Conclusion endophyte fungus T. present showed high biocontrol potential caused via direct parasitism or metabolites, positive roles promoting growth.
Language: Английский
Citations
5
Postharvest Biology and Technology, Journal Year: 2024, Volume and Issue: 217, P. 113104 - 113104
Published: July 18, 2024
Language: Английский
Citations
5
Plant and Soil, Journal Year: 2024, Volume and Issue: unknown
Published: July 3, 2024
Language: Английский
Citations
4
Applied Microbiology, Journal Year: 2025, Volume and Issue: 5(1), P. 2 - 2
Published: Jan. 2, 2025
Ralstonia solanacearum is a bacterial pathogen that causes wilt in plants, resulting significant economic losses worldwide. Biological control mainly utilizes Bacillus spp. one of the most effective methods to prevent this disease. In work, strain stercoris TY-12 with an obvious antagonism effect on R. was screened, and inhibition diameter against reached 2.18 cm by plate test. Furthermore, antimicrobial protein isolated purified from fermentation supernatant TY-12. The LC-MS/MS analysis results indicated member M42 family metallopeptidase molecular weight approximately 40 kDa named MP-TY12. After co-culture MP-TY12 for 4 h, cell surface disrupted under SEM, indicating may inhibit growth enzymatically cleaving peptide bonds within wall or membrane structure via hydrolysis. To evaluate potential application disease during crop production, biocontrol efficacy capsicum infected investigated achieved 84.18%. promotion tests showed dry weight, fresh stem diameter, length, root chlorophyll content using obviously increased compared blank control. It suggested could be used as new microbial production might developed agent.
Language: Английский
Citations
0
Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)
Published: Jan. 3, 2025
Endophytes from medicinal plants are potential biocontrol agents against Fusarium oxysporum f. sp. cubense (Foc), which is the causative fungus of banana wilt disease. In present study, endophytic bacterium was isolated Globba racemosa and their antagonistic activities Foc were studied, probable molecular mechanism antagonism predicted by docking studies. The 16SrRNA sequencing confirmed isolate to be Bacillus velezensis CBMB205 (EG2). isolates distortion fungal hyphae illustrated in SEM. metabolites identified FTIR, suggesting presence C-H, CH3 O-H groups. Two major such as β-amyrin dihydroxy octadecenoic acid (DA) LC-MS analysis. Molecular studies suggested that these showed binding with chitin synthase 1 1,3-glucan pathogenic fungi. energy (BE) interaction between synthase-1 (CS-1), (1,3-GS) estimated -10.17 kcal/mol − 9.5 kcal/mol, respectively. BE CS-1 1,3-GS determined -2.43 3.4 current study demonstrated EG2 towards provided a silico also provides insight into developing metabolite-based antifungal for various agricultural applications.
Language: Английский
Citations
0
Microbiological Research, Journal Year: 2025, Volume and Issue: 293, P. 128054 - 128054
Published: Jan. 7, 2025
Language: Английский
Citations
0
Food Control, Journal Year: 2025, Volume and Issue: unknown, P. 111160 - 111160
Published: Jan. 1, 2025
Language: Английский
Citations
0
Environmental Microbiome, Journal Year: 2025, Volume and Issue: 20(1)
Published: Feb. 6, 2025
Abstract The shade avoidance response triggers a dramatic promotion of elongation growth, accompanied by significant reprogramming metabolic pathways as plants seek to prevent overtopping and adapt vegetative shade. Here we demonstrate that simulated results in changes the structure composition phyllosphere bacterial microbiome. Our study uncovered shifts diversity, occurrence, abundance activity bacteria within A comparison responses both wild-type phytochrome mutants, which inherently exhibit shade-avoidance phenotype, revealed indirect host plant physiology direct light among microbiota. Hierarchical clustering patterns further suggested over third taxa constituting core microbiome our assay show some degree Bacteria increased on with phenotype corresponded genera associated beneficial traits such enhanced disease resistance growth promotion. findings suggests manipulate their under conditions strategy optimise fitness when competing for light. We discuss implications terms furthering understanding plant-microbe signalling shaping possibility manipulating health an agricultural setting at high planting densities.
Language: Английский
Citations
0
Published: Jan. 1, 2025
Language: Английский
Citations
0
Plant and Soil, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 20, 2025
Language: Английский
Citations
0