Infertility in Men: Advances towards a Comprehensive and Integrative Strategy for Precision Theranostics

Cells, Journal Year: 2022, Volume and Issue: 11(10), P. 1711 - 1711

Published: May 22, 2022

Male infertility is an increasing and serious medical concern, though the mechanism remains poorly understood. Impaired male reproductive function affects approximately half of infertile couples worldwide. Multiple factors related to environment, genetics, age, comorbidities have been associated with impaired sperm function. Present-day clinicians rely primarily on standard semen analysis diagnose potential develop treatment strategies. To address quality assessment bias enhance accuracy, World Health Organization (WHO) has recommended standardized testing; however, conventional diagnostic therapeutic options for infertility, including physical examination analysis, remain ineffective in relieving social burden. Instead, assisted techniques are becoming primary approach. In post-genomic era, multiomics technologies that deeply interrogate genome, transcriptome, proteome, and/or epigenome, even at single-cell level, besides breakthroughs robotic surgery, stem cell therapy, big data, offer promises towards solving deterioration factor infertility. This review highlights complex etiology especially roles lifestyle environmental factors, discusses advanced technologies/methodologies used characterizing its pathophysiology. A comprehensive combination these innovative approaches a global multi-centric setting fulfilling suitable ethical consent could ensure optimal developmental outcomes. These combinatorial should allow development markers, molecular stratification classes, personalized Since choices influence fertility, their integration any approach required safe, proactive, cost-effective, noninvasive precision theranostics affordable, accessible, facilitate realizing procreation dream.

Language: Английский

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Probiotic Incorporation into Yogurt and Various Novel Yogurt-Based Products

Applied Sciences, Journal Year: 2022, Volume and Issue: 12(24), P. 12607 - 12607

Published: Dec. 8, 2022

Probiotics are commonly added to yogurt provide many health benefits for the consumer. A description is provided some used probiotics in yogurt. GRAS (generally recognized as safe) list of probiotic bacteria that can be or similar types products provided. Additionally, prebiotics, synbiotics (combination prebiotics and probiotics), postbiotics, paraprobiotics, psychobiotics Probiotic come various forms addition spoonable yogurt, an ingredient other food products. Many useful functional ingredients applied The safety must addressed, especially critically ill patients susceptible populations. survive within throughout its entire shelf-life gastrointestinal tract after consumption by consumer benefits, techniques maintain survival Furthermore, Greek acid whey. opportunities exist adding a wide variety yogurt-based

Language: Английский

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Gut microbiota supports male reproduction via nutrition, immunity, and signaling

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 13

Published: Aug. 18, 2022

Gut microbiota (GM) is a major component of the gastrointestinal tract. Growing evidence suggests that it has various effects on many distal organs including male reproductive system in mammals. GM and testis form gut-testis axis involving production key molecules through microbial metabolism or de novo synthesis. These have nutrition, immunity, hormone-related functions promote via circulatory system. helps maintain integral structure testes regulates testicular immunity to protect spermatogenic environment. Factors damaging negatively impact function, however, related mechanism unknown. Also, correlation between remains be yet investigated. This review discusses complex influence highlighting fertility.

Language: Английский

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Optimum Doses and Forms of Selenium Maintaining Reproductive Health via Regulating Homeostasis of Gut Microbiota and Testicular Redox, Inflammation, Cell Proliferation, and Apoptosis in Roosters

Journal of Nutrition, Journal Year: 2023, Volume and Issue: 154(2), P. 369 - 380

Published: Dec. 19, 2023

Language: Английский

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Heat stress-induced dysbiosis of the gut microbiota impairs spermatogenesis by regulating secondary bile acid metabolism in the gut

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 937, P. 173305 - 173305

Published: May 20, 2024

Heat stress (HS) poses a substantial challenge to livestock. Studies have demonstrated that HS reduces fertility and leads gut microbiota dysbiosis in bulls. However, the impact of on bulls during is still unclear. Our research revealed exposure decreased semen quality bulls, fecal transplantation (FMT) from heat-stressed recipient mice resulted significant decrease number testicular germ cells epididymal sperm. Untargeted metabolomics methodology 16S rDNA sequencing conjoint analysis Akkermansia muciniphila (A. muciniphila) seemed be key bacterial regulator spermatogenesis after exposure. Moreover, indicated A. regulated secondary bile acid metabolism by promoting colonization salt hydrolase (BSH)-metabolizing bacteria, leading increase retinol absorption host subsequently elevation retinoic level, thereby improving spermatogenesis. This study sheds light relationship between HS-induced spermatogenesis, offering potential therapeutic approach for addressing bull spermatogenic dysfunction triggered

Language: Английский

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Gut microbiota is involved in male reproductive function: a review

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: May 3, 2024

Globally, ~8%−12% of couples confront infertility issues, male-related issues being accountable for 50%. This review focuses on the influence gut microbiota and their metabolites male reproductive system from five perspectives: sperm quality, testicular structure, sex hormones, sexual behavior, probiotic supplementation. To improve can secrete by themselves or regulate host metabolites. Endotoxemia is a key factor in structure damage that causes orchitis disrupts blood-testis barrier (BTB). In addition, hormone levels participating synthesis hormone-related enzymes directly enterohepatic circulation affect hypothalamic-pituitary-testis (HPT) axis. They also activate areas brain control arousal behavior through Probiotic supplementation function. Therefore, may function behavior; however, further research needed to better understand mechanisms underlying microbiota-mediated infertility.

Language: Английский

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Contribution of the seminal microbiome to paternal programming

Biology of Reproduction, Journal Year: 2024, Volume and Issue: 111(2), P. 242 - 268

Published: May 2, 2024

Abstract The field of Developmental Origins Health and Disease has primarily focused on maternal programming offspring health. However, emerging evidence suggests that paternal factors, including the seminal microbiome, could potentially play important roles in shaping developmental trajectory long-term health outcomes. Historically, microbes present semen were regarded as inherently pathogenic agents. this dogma recently been challenged by discovery a diverse commensal microbial community within healthy males. In addition, recent studies suggest transmission semen-associated into female reproductive tract during mating potentials to not only influence fertility embryo development but also contribute offspring. review, we summarize current knowledge microbiota both humans animals followed discussing their potential involvement We propose discuss mechanisms through which influences are transmitted via microbiome. Overall, review provides insights microbiome-based programing, will expand our understanding currently epigenetic modifications, oxidative stresses, cytokines.

Language: Английский

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Sex-Specific Mechanisms of Fluoride-Induced Gonadal Injury: A Multi-Omics Investigation into Reproductive Toxicity and Gut Microbiota Disruption

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 16, 2025

Fluoride, a common agricultural additive used to enhance plant resilience and pest control, poses toxicity risks when exposure surpasses safe thresholds, affecting ecosystems human health. While its reproductive is recognized, the sex-specific cross-generational effects remain underexplored. To address this gap, we employed an integrative approach combining transcriptomics (next-generation sequencing (NGS)), bioinformatic network analysis, gut microbiota sequencing, in vivo functional assays. ICR mice (F0 generation), both male female, were exposed fluoride (100 mg/L drinking water) for 35 days, continuing through gestation offspring weaning. Our transcriptomic analysis revealed significant upregulation of autophagy (via PI3K-AKT-mTOR pathway) oxidative stress-induced mitochondrial dysfunction gonadal tissue, with more pronounced observed males. Further integrated analyses metabolomic data, supported by experiments, highlighted stress, dysfunction, pathway activation stronger The principal component confirmed transcriptome alterations, males showing substantial disruption. Additionally, 16S rRNA identified dysbiosis, particularly males, increased Firmicutes/Bacteroidetes ratio higher abundances Oscillospirales Anaerovoracaceae. Moreover, our study correlations between specific (e.g., Firmicutes, Proteobacteria) autophagy, pathways, notable sex-dependent differences. These findings suggest that may play critical role modulating fluoride-induced toxicity, their on stress cellular homeostasis. breakdown barrier elevated serum/gonadal lipopolysaccharide (LPS) levels fluoride-treated further established link dysbiosis toxicity. underscore importance considering sex differences xenobiotic-induced developmental

Language: Английский

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Seminal and vagino-uterine microbiome and their individual and interactive effects on cattle fertility

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 13

Published: Nov. 8, 2022

Reproductive failure is a major economical drain on cow-calf operations across the globe. It can occur in both males and females stem from prenatal postnatal influences. Therefore, cattle industry has been making efforts to improve fertility pregnancy rate herds as an attempt maintain sustainability profitability of production. Despite advancements made genetic selection, nutrition, implementation various reproductive technologies, rates have not significantly improved past 50 years. This signifies missing factor or factors current management practices that influence successful fertilization pregnancy. Emerging lines evidence derived human other animals including suggest microbial continuum along male female tracts are associated with fertility—that is, fertilization, implantation, success—highlighting potential for harnessing microbiome cattle. The objective this narrative review provide overview recent studies bovine seminal vagino-uterine discuss individual interactive roles these communities defining fertility.

Language: Английский

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Pharmacological Activity of Flavonoid Quercetin and Its Therapeutic Potential in Testicular Injury

Nutrients, Journal Year: 2023, Volume and Issue: 15(9), P. 2231 - 2231

Published: May 8, 2023

Quercetin is a natural flavonoid widely found in fruits and vegetables. Recent studies have shown that quercetin mediates multiple beneficial effects variety of organ damage diseases, considered healthcare supplement with health-promoting potential. Male infertility major health concern, testicular from causes an important etiology. Previous has protective effect on reproductive function. This may be related to the antioxidant, anti-inflammatory, anti-apoptotic biological activities quercetin. Therefore, this paper reviews mechanisms by which exerts its pharmacological activity role induced various etiologies. In addition, compiles application clinical trials, demonstrating practical regulating blood pressure inhibiting cellular senescence human patients. However, more in-depth experimental trials are needed confirm true value for prevention protection against injury.

Language: Английский

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