Reproductive Microbiomes in Domestic Livestock: Insights Utilizing 16S rRNA Gene Amplicon Community Sequencing

Animals, Journal Year: 2023, Volume and Issue: 13(3), P. 485 - 485

Published: Jan. 31, 2023

Advancements in 16S rRNA gene amplicon community sequencing have vastly expanded our understanding of the reproductive microbiome and its role fertility. In humans, Lactobacillus is overwhelmingly dominant bacteria within tissues known to be commensal an indicator fertility women men. It also that not as largely abundant domestic livestock species. Thus, objective this review summarize research date on both female male microbiomes species (i.e., dairy cattle, beef swine, small ruminants, horses). Having a comprehensive microbiota modulating physiological functions will aid development management therapeutic strategies improve efficiency.

Language: Английский

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Contribution of the seminal microbiome to paternal programming

Biology of Reproduction, Journal Year: 2024, Volume and Issue: 111(2), P. 242 - 268

Published: May 2, 2024

Abstract The field of Developmental Origins Health and Disease has primarily focused on maternal programming offspring health. However, emerging evidence suggests that paternal factors, including the seminal microbiome, could potentially play important roles in shaping developmental trajectory long-term health outcomes. Historically, microbes present semen were regarded as inherently pathogenic agents. this dogma recently been challenged by discovery a diverse commensal microbial community within healthy males. In addition, recent studies suggest transmission semen-associated into female reproductive tract during mating potentials to not only influence fertility embryo development but also contribute offspring. review, we summarize current knowledge microbiota both humans animals followed discussing their potential involvement We propose discuss mechanisms through which influences are transmitted via microbiome. Overall, review provides insights microbiome-based programing, will expand our understanding currently epigenetic modifications, oxidative stresses, cytokines.

Language: Английский

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Role of the placenta in developmental programming: Observations from models using large animals

Animal Reproduction Science, Journal Year: 2023, Volume and Issue: 257, P. 107322 - 107322

Published: Aug. 30, 2023

Language: Английский

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Developmental programming of reproduction in the female animal

Animal Reproduction Science, Journal Year: 2024, Volume and Issue: 263, P. 107456 - 107456

Published: March 16, 2024

Language: Английский

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Whole-body microbiota of newborn calves and their response to prenatal vitamin and mineral supplementation

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: June 26, 2023

Early life microbial colonization and factors affecting patterns are gaining interest due to recent developments suggesting that early microbiome may play a role in Developmental Origins of Health Disease. In cattle, limited information exists on the anatomical sites involved bovine health beyond gastrointestinal tract. Here, we investigated 1) initial seven different locations newborn calves 2) whether these communities 3) serum cytokine profiles influenced by prenatal vitamin mineral (VTM) supplementation. Samples were collected from hoof, liver, lung, nasal cavity, eye, rumen (tissue fluid), vagina beef born dams either received or did not receive VTM supplementation throughout gestation (n = 7/group). Calves separated immediately after birth fed commercial colostrum milk replacer until euthanasia at 30 h post-initial feeding. The microbiota all samples was assessed using 16S rRNA gene sequencing qPCR. Calf subjected multiplex quantification 15 cytokines chemokines. Our results indicated colonized site-specific microbiota, whose community structure differed ruminal-associated (0.64 ≥ R2 0.12, p ≤ 0.003). ruminal fluid only one treatment (p < 0.01). However, differences 0.05) detected richness (vagina); diversity (ruminal tissue, fluid, eye); composition phylum genus level vagina); total bacterial abundance (eye vagina). From evaluated, concentration chemokine IP-10 greater 0.02) compared control calves. Overall, our suggest upon birth, whole-body relatively rich, diverse, communities. Noticeable observed ruminal, vaginal, ocular response These findings can derive future hypotheses regarding body sites, maternal micronutrient consumption as factor influence colonization.

Language: Английский

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Paternal effects on fetal programming

Animal Reproduction, Journal Year: 2023, Volume and Issue: 20(2)

Published: Jan. 1, 2023

Paternal programming is the concept that environmental signals from sire's experiences leading up to mating can alter semen and ultimately affect phenotype of resulting offspring. Potential mechanisms carrying paternal effects offspring be associated with epigenetic signatures (DNA methylation, histone modification non-coding RNAs), oxidative stress, cytokines, seminal microbiome. Several opportunities exist for sperm/semen influenced during development; these are within testicle, epididymis, or accessory sex glands. Epigenetic sperm impacted pre-natal pre-pubertal periods, sexual maturity advancing sire age. Sperm susceptible alterations as dictated by their developmental stage at time perturbation, plasma likely have both dependent independent on Research using rodent models has revealed many factors including over/under nutrition, dietary fat, protein, ingredient composition (e.g., macro- micronutrients), exercise, exposure drugs, alcohol, endocrine disruptors all elicit responses evident in phenotype. livestock species also age, fertility level, plane heat stress induce epigenetic, cytokine, microbiome profiles and/or plasma. In addition, recent findings pigs, sheep, cattle indicated blastocysts post-fertilization some continuing into post-natal life Our research group focused understanding common management scenarios nutrition growth rates bulls rams subsequent outcomes. Understanding implication imperative short-term feeding decisions potential impact productivity profitability our herds generations come.

Language: Английский

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Sequencing and culture-based characterization of the vaginal and uterine microbiota in beef cattle that became pregnant or remained open following artificial insemination

Microbiology Spectrum, Journal Year: 2023, Volume and Issue: 11(6)

Published: Nov. 3, 2023

Emerging evidence suggests that microbiome-targeted approaches may provide a novel opportunity to reduce the incidence of reproductive failures in cattle. To develop such microbiome-based strategies, one first logical steps is identify microbiome features related fertility and isolate fertility-associated microbial species for developing future bacterial consortium could be administered before breeding enhance pregnancy outcomes. Here, we characterized vaginal uterine microbiota beef cattle became pregnant or remained open via artificial insemination identified associated with fertility. We compared similarities between heifers cows. Using culturing, provided new insights into culturable fraction their antimicrobial resistance. Overall, our findings will serve as an important basis research aimed at harnessing improved

Language: Английский

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A Longitudinal Characterization of the Seminal Microbiota and Antibiotic Resistance in Yearling Beef Bulls Subjected to Different Rates of Gain

Microbiology Spectrum, Journal Year: 2023, Volume and Issue: 11(2)

Published: March 14, 2023

In this study, we evaluated the seminal and fecal microbiota in yearling beef bulls fed a common diet to achieve moderate (1.13 kg/day) or high (1.80 rates of weight gain. Semen samples were collected on days 0 112 dietary intervention (n = 19/group) as well postbreeding 6/group) using electroejaculation, was assessed 16S rRNA gene sequencing, quantitative PCR (qPCR), culturing. The also evaluated, its similarity with assessed. A subset bacterial isolates 33) screened for resistance against 28 antibiotics. complex dynamic detected bovine semen, community structure affected by sampling time (R2 0.16, P < 0.001). Microbial richness increased significantly from day 112, diversity after breeding (P > 0.05). Seminal remained unaffected differential gain, overall composition distinct microbiota, only 6% taxa shared between them. total 364 49 different genera recovered under aerobic anaerobic Among these pathogenic species those resistant several Overall, our results suggest that semen harbors rich which changes over during season but appears be resilient gains achieved via diet. is potentially antibiotic-resistant species. IMPORTANCE Increasing evidence human other animal supports existence commensal may influence not sperm quality fertility female reproduction. evolution factors shaping community, however, remain largely underexplored. characterized response bull age, gains, mating activity. We compared culturable bacteria their antimicrobial resistance. Our obtained culturing, antibiotic susceptibility testing provide novel information taxonomic composition, evolution, bulls. This will serve an important basis further understanding microbiome involvement reproductive health cattle.

Language: Английский

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A single intranasal dose of essential oil spray confers modulation of the nasopharyngeal microbiota and short-term inhibition of Mannheimia in feedlot cattle: a pilot study

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Jan. 8, 2024

Abstract Five essential oils (EOs) were previously characterized in vitro and identified as candidate EOs for the development of an intranasal EO spray to mitigate bovine respiratory disease (BRD) pathogens. In present study, these evaluated their potential (i) reduce BRD pathogens, (ii) modulate nasopharyngeal microbiota, (iii) influence animal performance, feeding behavior immune response when a single dose administered intranasally feedlot cattle. Forty beef steer calves (7–8 months old, Initial body weight = 284 ± 5 kg [SE]) received either (ajowan, thyme, fennel, cinnamon leaf, citronella) or PBS (Control; n 20/group) on day 0. Deep swabs collected days (d) -1, 1, 2, 7, 14, 28, 42 processed 16S rRNA gene sequencing, qPCR, culturing. Significant effects community structure (d1), microbial richness diversity, relative abundance some dominant phyla (d1, d2, d14), overall interaction network microbiota detected. The Mannheimia was lower (4.34%) than Control (10.4%) M. haemolytica prevalence d7 compared control calves. Feed intake, average daily gain, behavior, blood cell counts not affected by treatment. Overall, resulted moderate modulation short-term inhibition while influencing response. Our first time, shows use

Language: Английский

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Characterizing the prevalence of Fusobacterium necrophorum subsp. necrophorum, Fusobacterium necrophorum subsp. funduliforme, and Fusobacterium varium in bovine and ovine semen, bovine gut, and vagino-uterine and fetal microbiota using targeted culturing and qPCR

Microbiology Spectrum, Journal Year: 2025, Volume and Issue: unknown

Published: March 24, 2025

ABSTRACT Fusobacterium necrophorum is an important pathogen associated with several infectious diseases in cattle. However, recent sequencing-based studies reported that F. may be positively pregnancy beef cows and highly abundant bull seminal microbiota potential involvement reproductive health fertility. Here, we performed a comprehensive screening to (i) determine the prevalence of (subspecies [FNN] funduliforme [FNF]) varium (FV) cattle sheep as well bovine digestive tract ecosystems, (ii) explore whether these spp. colonize calf prenatally. For this, screened 11 different sample types including ram semen, vaginal uterine swabs, fecal samples, samples from 180- 260-day-old fetuses their respective dams using both quantitative PCR (qPCR; 514 samples) targeted culturing (499 samples). By qPCR, all were detected across varying rates viability. FNF was prevalent semen (66.7%) maternal ruminal fluids (87.1%), its viability confirmed through culturing. All identified swab (3.1%–9.4%), caruncles, fetal fluids, rumen, meconium (2.7%–26.3%) by qPCR but not isolated culture method. Overall, our results, for first time, suggest commensal member healthy male microbiota, FNF, FNN, FV are present vagino-uterine intestine IMPORTANCE Recent Fusobacterium, , known primary etiological agent cattle, non-pathogenic members pro-fertility effects. further information regarding absolute abundance, viability, higher taxonomic resolution species subspecies which cannot achievable amplicon sequencing approach needed confirm status over 500 qPCR. results provide novel insights into female tracts presence fetuses, will serve basis research understanding role

Language: Английский

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