Biology,
Journal Year:
2023,
Volume and Issue:
12(3), P. 409 - 409
Published: March 6, 2023
Ammonia
is
a
significant
concern
during
hatchery
culture
in
brachyuran
species,
and
its
accumulation
may
lead
to
abortive
moulting
large-scale
deaths
of
the
early
juveniles.
To
date,
underlying
mechanism
for
ammonia-induced
alteration
process
still
unknown.
In
this
study,
we
aimed
investigate
effects
ammonia
on
as
well
potential
mechanisms
juveniles
swimming
crab
Portunus
trituberculatus,
an
important
aquaculture
species
China.
We
evaluated
survival
rate
juvenile
crabs
(C2)
analyzed
expression
pattern
genes
key
components
molt
signaling
complete
cycle
under
different
concentrations
nitrogen
(the
control
group:
<0.1
mg/L;
LA
5
HA
20
mg/L).
The
results
showed
that:
(1)
groups
was
lower
than
that
group
at
end
experiment,
death
syndrome
(MDS)
only
observed
group;
(2)
higher
compared
(3)
consistent
with
MIH
decreased
expression,
related
ecdysteroid
synthesis,
receptors,
responsive
effectors
exhibited
increased
(4)
although
upregulated,
associated
receptors
downstream
group.
Our
indicated
low
levels
can
promote
by
inhibiting
activating
moult
signaling,
whereas
high
inhibit
MDS
through
impairing
signaling.
Antioxidants,
Journal Year:
2022,
Volume and Issue:
11(3), P. 440 - 440
Published: Feb. 22, 2022
Salinity
is
one
of
the
important
environmental
factors
affecting
survival
and
growth
aquatic
animals.
However,
impact
low-salinity
stress
on
M.
rosenbergii
post-larvae
at
different
development
stages
remains
elusive.
Therefore,
aim
this
study
was
to
explore
underlying
mechanisms
hypotonic
through
transcriptome
analysis
antioxidant
parameters
detection.
The
salinity
control
group
15
psu
(S15)
6
(S6).
Samples
were
collected
7
days-post-hatch
(dph),
14
dph
21
larvae.
results
showed
that
caused
oxidative
damage
in
evidenced
by
decreased
glutathione
peroxidase
(GSH-Px);
superoxide
dismutase
(SOD);
anti-superoxide
anion
free
radical
(ASAFR);
increased
malondialdehyde
(MDA);
nitric
oxide
(NO);
inducible
synthase
(iNOS)
levels.
Transcriptome
there
1428,
1187,
132
DEGs
including
301,
366,
4
up-regulated
genes
1127,
821,
128
down-regulated
dph,
larvae
under
stress,
respectively.
Furthermore,
GO
KEGG
enrichment
indicated
led
dysregulation
immune
signals
lysosome
autophagy
autophagy-related
beclin
1-associated
key
regulator
(Barkor);
ubiquitin-like
modifier-activating
enzyme
ATG7
(ATG7);
Beclin;
protein
13
(ATG13);
nuclear
receptor-binding
factor
2
(Nrbf2);
ubiquitin-like-conjugating
ATG3
(ATG3);
vacuole
membrane
1
(VMP1);
(ATG2)
larvae,
then
stress.
In
renin-angiotensin
system
activated.
conclusion,
our
data
reduced
capacity
impaired
post-larvae,
but
as
progresses,
adaptability
gradually
increased,
might
reach
a
new
homeostasis
RAS
signaling
pathway.
Antioxidants,
Journal Year:
2023,
Volume and Issue:
12(10), P. 1836 - 1836
Published: Oct. 7, 2023
Salinity
stress
can
trigger
a
series
of
physiological
changes.
However,
the
mechanism
underlying
response
to
acute
salinity
in
Macrobrachium
rosenbergii
remains
poorly
understood.
In
this
study,
osmoregulation,
metabolism,
antioxidant
capacity,
and
apoptosis
were
examined
over
96
h
stress.
Hemolymph
osmolality
increased
with
increasing
salinity.
After
48
exposure,
glucose,
triglycerides,
total
protein,
cholesterol
contents
two
groups
(13
26‰
salinity)
significantly
lower
than
those
0‰
group.
The
highest
levels
these
parameters
detected
at
6
h;
however,
superoxide
dismutase
(SOD),
capacity
(T-AOC),
malondialdehyde
(MDA)
lowest
13‰
activity
immunity-related
enzyme
alkaline
phosphatase
(AKP)
showed
decreasing
trend
remained
low
level
group
throughout
experiment.
No
significant
differences
observed
aspartate
aminotransferase
(AST),
alanine
(ALT),
or
lysozyme
(LZM)
among
three
treatments
h.
treatments,
gill
filament
diameter
decreased,
more
pronounced
terminal
deoxynucleotidyl
transferase
dUTP
nick
end
labeling
(TUNEL)-positive
signal
was
compared
that
Expression
apoptosis-related
genes,
including
Cysteine-aspartic
acid
protease
3
(Caspase
3),
8
8),
Cytochrome
c
(Cyt-c),
tumor
suppressor
gene
(P53),
Nuclear
factor
kappa-B
(NF-κB),
B
cell
lymphoma
2
ovarian
killer
(Bok)
higher
other
24
h,
but
Cyt-c
P53
exhibited
positive
relationship
MDA,
AST,
LZM
during
group,
Bok
expression
correlated
SOD,
T-AOC,
AKP,
phosphatase,
activity,
whereas
AST
content
positively
Caspase
8,
Cyt-c,
expression.
A
negative
between
catalase
(CAT)
activity.
These
findings
provide
insight
into
mechanisms
will
contribute
improving
M.
aquaculture
management
practices.
Frontiers in Endocrinology,
Journal Year:
2020,
Volume and Issue:
11
Published: Sept. 8, 2020
Neuropeptides
and
their
G
protein-coupled
receptors
(GPCRs)
from
the
central
nervous
system
regulate
physiological
responses
of
crustaceans.
However,
in
crustaceans,
our
knowledge
regarding
GPCR
expression
patterns
phylogeny
is
limited.
Thus,
present
study
aimed
to
analyze
eyestalk
transcriptome
oriental
river
prawn
Macrobrachium
nipponense
response
salinity
acclimation.
We
obtained
162,250
unigenes
after
de
novo
assembly,
1,392
1,409
differentially
expressed
genes
were
identified
prawns
low
high
salinity,
respectively.
used
combinatorial
bioinformatic
analyses
identify
M.
encoding
GPCRs
neuropeptides.
The
mRNA
levels
seven
neuropeptides
one
validated
acclimation
using
quantitative
real-time
reverse
transcription
polymerase
chain
reaction.
A
total
148
GPCR-encoding
transcripts
belonging
three
classes
identified,
including
77
GPCR-A
proteins,
52
GPCR-B
19
other
GPCRs.
results
increase
understanding
molecular
basis
neural
signaling
nipponense,
which
will
promote
further
research
into
this
crustacean.
Frontiers in Marine Science,
Journal Year:
2022,
Volume and Issue:
9
Published: April 20, 2022
Salinity
is
an
important
factor
affecting
the
survival,
growth,
and
metabolism
of
marine
crustaceans.
Low-salt
stress
will
result
in
death
swimming
crabs.
This
paper
investigates
metabolic
response
gills
Portunus
trituberculatus
under
short-term
low-salt
by
comparing
molecules
four
salinity
treatment
groups
(24‰,
16‰,
12‰,
8‰)
GC-MS
technique.
In
this
study,
nine
common
differential
metabolites
such
as
pyruvate,
malic
acid,
phosphoethanolamine
were
found
gill
tissues
KEGG
enrichment
analysis
revealed
that
six
pathways,
including
citric
acid
cycle,
pyruvate
metabolism,
HIF-1
signaling
system,
significantly
impacted
low
salt
stimulation.
According
to
findings,
12‰
a
critical
node
crab
adaptation
salinity.
process
low-salinity
environment,
amino
acids
participated
osmotic
regulation,
organic
involved
energy
ensure
their
supply.
research
further
enriched
theory
regulation
mechanism
crustaceans,
with
goal
providing
guidance
for
improvement
culture
technique
trituberculatus.
