Clinical and Experimental Ophthalmology, Journal Year: 2023, Volume and Issue: 51(6), P. 514 - 515
Published: Aug. 1, 2023
The authors declare no conflict of interest.
Language: Английский
Nature Reviews Bioengineering, Journal Year: 2024, Volume and Issue: 2(10), P. 829 - 848
Published: July 18, 2024
Language: Английский
Citations
4
Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 207 - 223
Published: Jan. 1, 2025
Language: Английский
Citations
0
Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 13
Published: Feb. 25, 2025
The study aimed to evaluate the effect of GeLMA/bFGF hydrogel loaded with dental pulp stem cells (DPSCs) on repair and regeneration traumatic optic nerve injury. was photo-cross-linked by LED light. physical-chemical properties cytocompatibility after being squeezed (GeLMA/bFGF-SQ) were evaluated SEM degradation analyses, as well live/dead CCK-8 assays, respectively. axon growth PC12 MAP2 staining. GeLMA/bFGF/DPSCs-SQ injected in situ into lesion site observe number retinal ganglion (RGCs) calculated βIII-tubulin length regenerated axons observed CTB Tissue recovery HE TEM GeLMA/bFGF-SQ had a porous structure great degradability, good cytocompatibility. Meanwhile, DPSCs-conditioned medium (DPSCs-CM) could promote cells. Moreover, RGCs highest group. data revealed abundant newly orderly arrangement that damaged area Transplantation provided an appropriate microenvironment for injured nerves. DPSCs combined bFGF protected from apoptosis promoted secreting series neurotrophic factors.
Language: Английский
Citations
0
Experimental Neurology, Journal Year: 2024, Volume and Issue: 379, P. 114877 - 114877
Published: June 27, 2024
Language: Английский
Citations
3
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(14), P. 7736 - 7736
Published: July 15, 2024
Neutrophils, traditionally viewed as first responders to infection or tissue damage, exhibit dynamic and diverse roles in ocular health disease. This review elaborates on previous findings that showed how neutrophils contribute diseases. In infections, play a pivotal role host defense by orchestrating inflammatory responses combat pathogens. Furthermore, optic nerve neuropathies retinal degenerative diseases like age-related macular degeneration (AMD) diabetic retinopathy (DR), are implicated neuroinflammation damage owing their ability undergo neutrophil extracellular trap formation (NETosis) secretion of molecules. Targeting neutrophil-dependent processes holds promise therapeutic strategy, offering potential avenues for intervention cancers, Understanding the multifaceted is crucial developing targeted therapies improve patient outcomes.
Language: Английский
Citations
3
Frontiers in Neurology, Journal Year: 2025, Volume and Issue: 15
Published: Jan. 9, 2025
Retinal ganglion cells (RGCs) generally fail to regenerate axons, resulting in irreversible vision loss after optic nerve injury. While many studies have shown that modulating specific genes can enhance RGCs survival and promote regeneration, inducing long-distance axon regeneration vivo through single-gene manipulation remains challenging. Nevertheless, combined multi-gene therapies proven effective significantly enhancing axonal regeneration. At present, research on promoting slow, with most unable achieve growth beyond the chiasm or reestablish connections brain. Future priorities include directing along correct pathways, facilitating synapse formation myelination, modifying inhibitory microenvironment. These strategies are crucial not only for but also broader applications central nervous system repair. In this review, we discuss multifactors therapeutic offering insights into advancing research.
Language: Английский
Citations
0
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown
Published: April 15, 2025
Abstract Retinal ganglion cells (RGCs) are the sole projection neurons connecting retina to brain and therefore play a critical role in vision. Death of RGCs during glaucoma, optic neuropathies after ocular trauma results irreversible loss vision as do not regenerate human eye. Moreover, there no FDA approved therapies that prevent RGC death and/or promote survival diseased or injured There is need better understand molecular underpinnings neuroprotection develop effective therapeutic approaches preserve damaged RGCs. Unlike mammals, zebrafish resilient nerve injury, even complete transection nerve. Here, we leveraged this unique model utilized single-cell RNA sequencing characterize responses injury identify putative neuroprotective regenerative pathways. heterogeneous studies mice have shown differential resiliency across subtypes. Our demonstrated all subtypes zebrafish. Quantifying changes gene expression revealed upregulation progenitor markers well distinct early late phases response. This shift causes injury-responsive resemble subtype 3, low frequency population endogenous immature normally maintained wild-type, uninjured adult retina. A similar but restricted transcriptomic response contralateral eye was also detected, highlighting systemic unilateral injury. Taken together, these demonstrate dedifferentiate may be novel mechanism mediating their cell capabilities. Author Summary connect essential for Their conditions like affecting over 70 million people worldwide, leads permanent blindness, with FDA-approved treatments it. In study, used next-generation technologies at level. We discovered survive damage by temporarily shifting into less mature state, resembling rare found animals. identified many genes whose RGCs, work significant because our detailed characterization identifies dedifferentiation an response, possibly important axon regrowth. The pathways potential targets enable
Language: Английский
Citations
0
Translational Vision Science & Technology, Journal Year: 2024, Volume and Issue: 13(8), P. 37 - 37
Published: Aug. 23, 2024
Purpose: Adeno-associated virus (AAV) demonstrates promise in delivering therapeutic genes to retinal ganglion cells (RGCs). Delivery of neuroprotective is constrained by packaging size and/or cell selectivity. This study compares the ability RGC-selective gamma-synuclein (SNCG) promoter and smaller neurofilament heavy chain (NEFH) promoter, as well portions atonal bHLH transcription factor 7 (ATOH7) enhancer, drive gene expression RGCs. Methods: AAV2 constructs with green fluorescent protein (GFP) or human sirtuin 1 (hSIRT1) driven cytomegalovirus (CMV) enhancer NEFH (AAV2–eCMV–NEFH) distal active sequences ATOH7 (DiATOH7) SNCG (AAV2–DiATOH7–SNCG) were intravitreally injected into C57BL/6J mice. RGCs immunolabeled Brn3a antibodies counted. AAV utmost transduction efficiency used test efficacy hSIRT1 12-week-old mice subjected microbead (MB)-induced intraocular pressure (IOP) elevation. Visual function was measured using optokinetic responses (OKRs). Results: The eGFP AAV2–eCMV–NEFH similar that AAV2–eCMV–SNCG AAV2–DiATOH7–SNCG. When combined a larger less efficient than shorter DiATOH7 enhancer. Similarly, AAV2–eCMV–SNCG. latter two vectors equally increasing RGC survival improving visual mouse model MB-induced IOP Conclusions: promoters represent comparable selective sequences; however, offers size. Translational Relevance: Smaller enhancer–promoter combinations can be deliver for treatment optic neuropathies including glaucoma.
Language: Английский
Citations
2
Investigative Ophthalmology & Visual Science, Journal Year: 2024, Volume and Issue: 65(10), P. 22 - 22
Published: Aug. 14, 2024
Purpose: Optic nerve (ON) injuries can result in vision loss via structural damage and cellular injury responses. Understanding the immune response, particularly role of macrophages, response to ON is crucial for developing therapeutic approaches which affect repair. The present study investigates macrophages fibrotic scar formation, retinal ganglion cell (RGC) function. Methods: utilizes macrophage Fas-induced apoptosis (MaFIA) mice selectively deplete hematogenous explores impact have on Histological immunofluorescence analyses were used evaluate expression levels formation. Pattern electroretinogram (PERG) recordings assess RGC function as injury. Results: Successful depletion was induced MaFIA mice, led reduced formation post-injury. Despite an increase activated retina, preserved, demonstrated by normal PERG waveforms up 2 months suggests a neuroprotective repair highlights complex Conclusions: To our knowledge, this first use demonstrate that targeted leads significant reduction size preservation functionality after These findings highlight key opens new avenues interventions injuries. Future research should focus investigating distinct roles subtypes potential macrophage-associated molecular targets improve regeneration
Language: Английский
Citations
0
Neuroscience Bulletin, Journal Year: 2024, Volume and Issue: unknown
Published: Aug. 27, 2024
Language: Английский
Citations
0