Annals of Translational Medicine,
Journal Year:
2024,
Volume and Issue:
12(5), P. 88 - 88
Published: Oct. 1, 2024
Osteoarthritis
(OA)
is
a
major
source
of
pain
and
disability
worldwide.
Understanding
disease
progression
evolving,
but
OA
increasingly
thought
to
be
multifactorial
in
which
the
innate
immune
system
plays
role
regulating
perpetuating
low-grade
inflammation.
The
aim
this
study
was
enhance
our
understanding
immunopathogenesis
through
characterization
transcriptomic
responses
joints,
with
goal
facilitate
development
targeted
therapies.
Cell Proliferation,
Journal Year:
2024,
Volume and Issue:
57(6)
Published: Jan. 10, 2024
Abstract
Osteoarthritis
(OA)
is
the
most
prevalent
disorder
of
synovial
joint
affecting
multiple
joints.
In
past
decade,
we
have
witnessed
conceptual
switch
OA
pathogenesis
from
a
‘wear
and
tear’
disease
to
entire
joint.
Extensive
studies
been
conducted
understand
underlying
mechanisms
using
genetic
mouse
models
ex
vivo
tissues
derived
individuals
with
OA.
These
revealed
that
signalling
pathways
are
involved
in
development,
including
canonical
Wnt/β‐catenin
its
interaction
other
pathways,
such
as
transforming
growth
factor
β
(TGF‐β),
bone
morphogenic
protein
(BMP),
Indian
Hedgehog
(Ihh),
nuclear
κB
(NF‐κB),
fibroblast
(FGF),
Notch.
The
identification
currently
underway
specific
molecule(s)
key
pathway(s)
playing
decisive
role
development
need
be
evaluated.
This
review
will
focus
on
recent
progresses
understanding
critical
β‐catenin
TGF‐β,
BMP,
Notch,
Ihh,
NF‐κB,
FGF.
Understanding
these
novel
insights
into
integration
complex
gene
regulatory
network
during
help
us
identify
pathway
leading
discovery
therapeutic
strategies
for
intervention.
Military Medical Research,
Journal Year:
2024,
Volume and Issue:
11(1)
Published: May 30, 2024
Abstract
Orthopedic
conditions
have
emerged
as
global
health
concerns,
impacting
approximately
1.7
billion
individuals
worldwide.
However,
the
limited
understanding
of
underlying
pathological
processes
at
cellular
and
molecular
level
has
hindered
development
comprehensive
treatment
options
for
these
disorders.
The
advent
single-cell
RNA
sequencing
(scRNA-seq)
technology
revolutionized
biomedical
research
by
enabling
detailed
examination
diversity.
Nevertheless,
investigating
mechanisms
in
highly
mineralized
skeletal
tissue
poses
technical
challenges.
In
this
review,
we
present
a
streamlined
approach
to
obtaining
high-quality
single
cells
from
provide
an
overview
existing
scRNA-seq
technologies
employed
studies
along
with
practical
bioinformatic
analysis
pipelines.
By
utilizing
methodologies,
crucial
insights
into
developmental
dynamics,
maintenance
homeostasis,
involved
spine,
joint,
bone,
muscle,
tendon
disorders
been
uncovered.
Specifically
focusing
on
joint
diseases
degenerative
disc
disease,
osteoarthritis,
rheumatoid
arthritis
using
provided
novel
more
nuanced
comprehension.
These
findings
paved
way
discovering
therapeutic
targets
that
offer
potential
benefits
patients
suffering
diverse
Signal Transduction and Targeted Therapy,
Journal Year:
2025,
Volume and Issue:
10(1)
Published: Feb. 4, 2025
Abstract
Research
on
treating
knee
osteoarthritis
(KOA)
is
becoming
more
challenging
due
to
a
growing
number
of
younger
patients
being
affected.
The
pathogenesis
KOA
complex
for
multifactorial
disease
affecting
the
entire
joint,
with
remodeling
subchondral
bone
playing
key
role
in
degeneration
overlying
cartilage.
Therefore,
this
study
constructed
bipedal
postmenopausal
mouse
model
better
understand
how
interplay
between
and
cartilage
contributes
development.
A
single-cell
atlas
osteochondral
composite
tissue
was
established.
Furthermore,
three
novel
subtypes
chondrocytes,
including
Smoc2
+
angiogenic
Angptl7
Col1a1
osteogenic
were
identified
femoral
condyles
mice.
In
addition,
chondrocytes
promoted
angiogenesis
mice
by
interacting
endothelial
cells
via
FGF2-FGFR2
signaling
pathway.
H-type
vessels
increased
bone,
recruiting
osteoprogenitor
facilitating
osteogenesis
Sparc
osteoblasts
have
negatively
regulated
mineralization
osteoblastic
differentiation,
aggravated
pathological
progression
KOA.
above
findings
offered
new
targets
opened
up
an
avenue
therapeutic
intervention
Cell Proliferation,
Journal Year:
2023,
Volume and Issue:
56(12)
Published: June 14, 2023
Osteoarthritis
is
a
progressive
and
heterogeneous
joint
disease
with
complex
pathogenesis.
The
various
phenotypes
associated
each
patient
suggest
that
better
subgrouping
of
tissues
genotypes
in
different
phases
osteoarthritis
may
provide
new
insights
into
the
onset
progression
disease.
Recently,
single-cell
RNA
sequencing
was
used
to
describe
pathogenesis
on
high-resolution
view
surpassing
traditional
technologies.
Herein,
this
review
summarizes
microstructural
changes
articular
cartilage,
meniscus,
synovium
subchondral
bone
are
mainly
due
crosstalk
amongst
chondrocytes,
osteoblasts,
fibroblasts
endothelial
cells
during
progression.
Next,
we
focus
promising
targets
discovered
by
its
potential
applications
target
drugs
tissue
engineering.
Additionally,
limited
amount
research
evaluation
bone-related
biomaterials
reviewed.
Based
pre-clinical
findings,
elaborate
clinical
values
for
therapeutic
strategies
osteoarthritis.
Finally,
perspective
future
development
patient-centred
medicine
therapy
combining
other
multi-omics
technologies
discussed.
This
will
cellular
level
field
personalized
therapeutics
future.
Journal of Materials Chemistry B,
Journal Year:
2024,
Volume and Issue:
12(17), P. 4148 - 4161
Published: Jan. 1, 2024
Cyaonoside
A
(CyA),
derived
from
the
natural
Chinese
medicine,
Cyathula
officinalis
Kuan,
which
was
for
a
long
time
used
to
treat
knee
injuries
and
relieve
joint
pain
in
traditional
showed
an
unclear
mechanism
protecting
cartilage.
In
addition,
CyA
poorly
hydrosoluble
incapable
of
being
injected
directly
into
cavity,
limited
its
clinical
application.
This
study
reveals
that
resisted
IL-1β-mediated
chondrogenic
inflammation
apoptosis.
Next,
transcriptome
sequencing
is
explore
potential
mechanisms
underlying
regulation
MSC
differentiation.
Based
on
these
findings,
CyA-loaded
composite
hydrogel
microspheres
(HLC)
were
developed
they
possessed
satisfactory
loading
efficiency,
suitable
degradation
rate
good
biocompatibility.
HLC
increased
anabolic
gene
(Acan,
COL2A,
SOX9)
expression,
while
downregulating
expression
catabolic
marker
MMP13
vitro.
osteoarthritis
mouse
model,
demonstrated
promising
therapeutic
capabilities
by
integrity
articular
conclusion,
this
provides
insights
regulatory
chondrocytes
proposes
microsphere-based
advanced
strategy
osteoarthritis.
Journal of Inflammation Research,
Journal Year:
2025,
Volume and Issue:
Volume 18, P. 1459 - 1470
Published: Jan. 1, 2025
The
prevalence
of
osteoarthritis
(OA),
the
most
common
chronic
joint
condition,
is
increasing
due
to
aging
population
and
escalating
obesity
rates,
leading
a
significant
impact
on
human
health
well-being.
Thus,
analyzing
key
targets
OA
through
bioinformatics
can
help
discover
new
biomarkers
improve
its
diagnosis.
microarray
RNA-seq
results
were
screened
from
Gene
Expression
Omnibus
(GEO)
database.
Functional
enrichment
analyses,
protein-protein
interaction
(PPI)
analysis,
weighted
gene
co-expression
network
analysis
(WGCNA)
DEGs
performed.
RT-qPCR
WB
further
performed
verify
hub
expression
in
rat.
In
this
study,
35
genes
identified
differential
using
GSE169077
GSE114007
datasets.
Enrichment
revealed
that
these
predominantly
enriched
HIF-1
signaling
pathway,
ECM-receptor
interaction,
FoxO
pathway.
Through
integration
validation
animal
models
ROC
curve
four
pivotal
(GADD45B,
CLDN5,
HILPDA
CDKN1B)
finally
identified.
conclusion,
could
serve
as
novel
for
predicting
treating
OA,
offering
fresh
insights
into
etiology
pathogenesis.
