A kinase translocation reporter reveals real-time dynamics of ERK activity in Drosophila DOI Creative Commons
Alice C. Yuen, Anadika R. Prasad, Vilaiwan M. Fernandes

et al.

Biology Open, Journal Year: 2022, Volume and Issue: 11(5)

Published: May 15, 2022

ABSTRACT Extracellular signal-regulated kinase (ERK) lies downstream of a core signalling cascade that controls all aspects development and adult homeostasis. Recent developments have led to new tools image manipulate the pathway. However, visualising ERK activity in vivo with high temporal resolution remains challenge Drosophila. We adapted translocation reporter (KTR) for use Drosophila, which shuttles out nucleus when phosphorylated by ERK. show ERK-KTR faithfully reports endogenous developing tissues, it responds genetic perturbations upstream Using time-lapse imaging, we made two novel observations: firstly, sustained hyperactivation expression dominant-active epidermal growth factor receptor raised overall level but did not alter kinetics activity; secondly, direction migration retinal basal glia correlated their levels, suggesting an explanation heterogeneity observed fixed tissue. Our results KTR technology can be applied Drosophila monitor real-time suggest this modular tool further study other kinases. This article has associated First Person interview first author paper.

Language: Английский

Insulin-Like Peptides and Cross-Talk With Other Factors in the Regulation of Insect Metabolism DOI Creative Commons
Szymon Chowański, Karolina Walkowiak‐Nowicka, Magdalena Joanna Winkiel

et al.

Frontiers in Physiology, Journal Year: 2021, Volume and Issue: 12

Published: June 29, 2021

The insulin-like peptide (ILP) and growth factor (IGF) signalling pathways play a crucial role in the regulation of metabolism, development, fecundity, stress resistance, lifespan. ILPs are encoded by multigene families that expressed nervous non-nervous organs, including midgut, salivary glands, fat body, tissue- stage-specific manner. Thus, more multidirectional complex control insect metabolism can occur. not only factors regulate metabolism. interact many cross-talk interactions different factors, for example, hormones (peptide nonpeptide), neurotransmitters factors. These observed at levels, three appear to be most prominent/significant: (1) coinfluence other on same target cells, (2) influence synthesis/secretion regulating (3) activity cells producing/secreting various For brain insulin-producing co-express sulfakinins (SKs), which cholecystokinin-like peptides, another key regulator express receptors tachykinin-related next involved It was also shown Drosophila melanogaster directly indirectly AKH. This review presents an overview regulatory peptides how these with players its regulation.

Language: Английский

Citations

67
Signaling Pathways That Regulate the Crustacean Molting Gland DOI Creative Commons
Donald L. Mykles

Frontiers in Endocrinology, Journal Year: 2021, Volume and Issue: 12

Published: June 21, 2021

A pair of Y-organs (YOs) are the molting glands decapod crustaceans. They synthesize and secrete steroid hormones (ecdysteroids) their activity is controlled by external internal signals. The YO transitions through four physiological states over molt cycle, which mediated molt-inhibiting hormone (MIH; basal state), mechanistic Target Rapamycin Complex 1 (mTORC1; activated Transforming Growth Factor-β (TGFβ)/Activin (committed ecdysteroid (repressed state) signaling pathways. MIH, produced in eyestalk X-organ/sinus gland complex, inhibits synthesis ecdysteroids. model for MIH organized into a cAMP/Ca

Language: Английский

Citations

58
Regulation of trehalose metabolism in insects: from genes to the metabolite window DOI
Meenakshi B. Tellis, Hemlata M. Kotkar, Rakesh S. Joshi

et al.

Glycobiology, Journal Year: 2023, Volume and Issue: 33(4), P. 262 - 273

Published: Feb. 10, 2023

Abstract Trehalose is a major circulatory sugar in the haemolymph of insects. It provides instant energy and protection against stress. metabolism associated with insect growth development. The architecture spatio-temporal expression dynamics trehalose transport genes are key for regulation. These controlled by various transcription factors, largely linked to nutrition, development, metamorphosis. Also, levels affected substrate affinities modifications enzymes involved pathway. A feedback mechanism involving precursors products can regulate metabolism. Further, neuroendocrine system controls under normal stressed conditions producing different hormones. Hypotrehalosemic hormones work surplus activate uptake degradation. In contrast, hypertrehalosemic stimulate production fat body its haemolymph. However, regulation insects needs be studied detail. This review discusses aspects synthesis, transport, degradation developmental transition stress response. Unraveling epigenetic transcriptional control chemical or genetic modulators provide further insights into intricate development- tissue-specific manner. molecular information about effectors regulators applied developing diverse biotechnological applications.

Language: Английский

Citations

35
The diverse roles of insulin signaling in insect behavior DOI Creative Commons

Anastasia A. Weger,

Clare C. Rittschof

Frontiers in Insect Science, Journal Year: 2024, Volume and Issue: 4

Published: April 4, 2024

In insects and other animals, nutrition-mediated behaviors are modulated by communication between the brain peripheral systems, a process that relies heavily on insulin/insulin-like growth factor signaling pathway (IIS). Previous studies have focused mechanistic physiological functions of insulin-like peptides (ILPs) in critical developmental adult milestones like pupation or vitellogenesis. Less work has detailed mechanisms connecting ILPs to nutrient-mediated related survival reproductive success. Here we briefly review range linked IIS insects, from conserved regulation feeding behavior evolutionarily derived polyphenisms. Where possible, incorporate information Drosophila melanogaster model species describe molecular neural connect nutritional status behavioral expression via IIS. We identify knowledge gaps which include diverse functional roles ILPs, how modulate function across lifespan, lack research broad taxa. Addressing these would enable better understanding evolution this widely deployed tool kit pathway.

Language: Английский

Citations

12
Drosophila melanogaster as a Model Organism for Obesity and Type-2 Diabetes Mellitus by Applying High-Sugar and High-Fat Diets DOI Creative Commons
Nieves Baenas, Anika E. Wagner

Biomolecules, Journal Year: 2022, Volume and Issue: 12(2), P. 307 - 307

Published: Feb. 14, 2022

Several studies have been published introducing Drosophila melanogaster as a research model to investigate the effects of high-calorie diets on metabolic dysfunctions. However, differences between use high-sugar (HSD) and high-fat (HFD) affect fly physiology, well influence sex age, seldom described. Thus, aim present work was compare HSD (30% sucrose) HFD (15% coconut oil) symptoms dysfunction related obesity type-2 diabetes mellitus, including weight gain, survival, climbing ability, glucose triglycerides accumulation expression levels insulin-like peptides (dIlps). Female male flies were subjected for 10, 20 30 days. The obtained results showed clear in both triglyceride dIlps expression, being gender age determinant. study also suggested that gain does not seem be an appropriate parameter define obesity, since other characteristics appear more meaningful development phenotypes. Taken together, demonstrate key role diets, HFD, induce obese phenotype with associated diseases. further are needed elucidate underlying molecular mechanisms how differently metabolism.

Language: Английский

Citations

33
Considerations for cultivated crustacean meat: potential cell sources, potential differentiation and immortalization strategies, and lessons from crustacean and other animal models DOI Creative Commons
Lisa Musgrove, Fraser D. Russell, Tomer Ventura

et al.

Critical Reviews in Food Science and Nutrition, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 25

Published: May 11, 2024

Cultivated crustacean meat (CCM) is a means to create highly valued shrimp, lobster, and crab products directly from stem cells, thus removing the need farm or fish live animals. Conventional enterprises face increasing pressures in managing overfishing, pollution, warming climate, so CCM may provide way ensure sufficient supply as global demand for these grows. To support development of CCM, this review briefly details cell culture work date, before addressing what presently known about muscle development, particularly molecular mechanisms involved, how might relate recent on cultivated production vertebrate species. Recognizing current lack lines available establish cultures, we also consider primary sources that can be obtained non-lethally including tissues limbs which are readily released regrown, putative cells circulating hemolymph. Molecular approaches inducing myogenic differentiation immortalization reviewed. Finally, assess status tools researchers, antibodies, propose avenues address existing shortfalls order see field progress.

Language: Английский

Citations

8
C. elegans insulin-like peptides DOI Creative Commons

Rain Zhu,

Ian D. Chin-Sang

Molecular and Cellular Endocrinology, Journal Year: 2024, Volume and Issue: 585, P. 112173 - 112173

Published: Feb. 11, 2024

Insulin-like peptides are a group of hormones crucial for regulating metabolism, growth, and development in animals. Invertebrates, such as C. elegans, have been instrumental understanding the molecular mechanisms insulin-like peptides. Here, we review 40 peptide genes encoded elegans genome. Despite large number, there is only one receptor, called DAF-2. The insulin growth factor signaling (IIS) pathway evolutionarily conserved from worms to humans. Thus provides an excellent model understand how these function. unique that it possesses antagonistic properties, unlike all human peptides, which agonists. This overview current literature on structures, processing, tissue localization, regulation. We will also provide examples during development, germline learning/memory, longevity.

Language: Английский

Citations

6
Behavioral state-dependent modulation of insulin-producing cells in Drosophila DOI Creative Commons
Sander Liessem, Martina Held, Rituja S Bisen

et al.

Current Biology, Journal Year: 2022, Volume and Issue: 33(3), P. 449 - 463.e5

Published: Dec. 28, 2022

Language: Английский

Citations

28
Insulin and Memory in Invertebrates DOI Creative Commons
Junko Nakai,

Nozomi Chikamoto,

Kanta Fujimoto

et al.

Frontiers in Behavioral Neuroscience, Journal Year: 2022, Volume and Issue: 16

Published: April 26, 2022

Insulin and insulin-like peptides (ILP) help to maintain glucose homeostasis, whereas growth factor (IGF) promotes the differentiation of cells in both vertebrates invertebrates. It is sometimes difficult distinguish between ILP IGF invertebrates, however, because some cases has same function as IGF. In present review, therefore, we refer these ILP/IGF signaling (IIS) discuss role IIS memory formation after classical conditioning arthropod Drosophila melanogaster , involved aversive olfactory memory, nematode Caenorhabditis elegans controls appetitive/aversive response NaCl depending on duration starvation. mollusk Lymnaea stagnalis a critical conditioned taste aversion. mammals also known play an important cognitive function, many studies humans have focused insulin potential treatment for Alzheimer’s disease. Although analyses tissue cellular levels progressed mammals, molecular mechanisms, such transcriptional translational levels, cognition been far advanced using We anticipate that review will pave way studying effects insulin, ILPs, IGFs across phyla.

Language: Английский

Citations

19
Metabolism and immune memory in invertebrates: are they dissociated? DOI Creative Commons

Texca T. Méndez-López,

Julio César Carrero, Humberto Lanz‐Mendoza

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: July 11, 2024

Since the discovery of specific immune memory in invertebrates, researchers have investigated its response to diverse microbial and environmental stimuli. Nevertheless, extent system’s interaction with metabolism, remains relatively enigmatic. In this mini review, we propose a comprehensive investigation into intricate interplay between metabolism memory. Our hypothesis is that cellular endocycles epigenetic modifications play pivotal roles shaping relationship. Furthermore, underscore importance crosstalk for understanding evolutionary costs. By evaluating these costs, can gain deeper insights adaptive strategies employed by invertebrates pathogenic challenges. Lastly, outline future research directions aimed at unraveling These avenues inquiry promise illuminate fundamental principles governing host-pathogen interactions trade-offs, thus advancing our invertebrate immunology.

Language: Английский

Citations

4