A kinase translocation reporter reveals real-time dynamics of ERK activity in Drosophila DOI Creative Commons
Alice C. Yuen, Anadika R. Prasad, Vilaiwan M. Fernandes

et al.

Biology Open, Journal Year: 2022, Volume and Issue: 11(5)

Published: May 15, 2022

ABSTRACT Extracellular signal-regulated kinase (ERK) lies downstream of a core signalling cascade that controls all aspects development and adult homeostasis. Recent developments have led to new tools image manipulate the pathway. However, visualising ERK activity in vivo with high temporal resolution remains challenge Drosophila. We adapted translocation reporter (KTR) for use Drosophila, which shuttles out nucleus when phosphorylated by ERK. show ERK-KTR faithfully reports endogenous developing tissues, it responds genetic perturbations upstream Using time-lapse imaging, we made two novel observations: firstly, sustained hyperactivation expression dominant-active epidermal growth factor receptor raised overall level but did not alter kinetics activity; secondly, direction migration retinal basal glia correlated their levels, suggesting an explanation heterogeneity observed fixed tissue. Our results KTR technology can be applied Drosophila monitor real-time suggest this modular tool further study other kinases. This article has associated First Person interview first author paper.

Language: Английский

Transcriptional control of a metabolic switch regulating cellular methylation reactions is part of a common response to stress in divergent bee species DOI Creative Commons

Helen V. Kogan,

Shannon G. MacLeod,

Nicole C. Rondeau

et al.

Journal of Experimental Biology, Journal Year: 2024, Volume and Issue: 227(11)

Published: May 13, 2024

Recent global declines in bee health have elevated the need for a more complete understanding of cellular stress mechanisms employed by diverse species. We recently uncovered biomarker lethal (2) essential life [l(2)efl] genes as part shared transcriptional program response to number cell stressors western honey (Apis mellifera). Here, we describe another stress-responsive gene, glycine N-methyltransferase (Gnmt), which is known key metabolic switch controlling methylation reactions. observed Gnmt induction both abiotic and biotic stressors. also found increased levels GNMT reaction product sarcosine midgut after stress, linking changes with gene regulation. Prior this study, upregulation had not been associated responses other organisms. To determine whether novel would behave similarly species, first characterized endoplasmic reticulum (ER) lab-reared adults solitary alfalfa leafcutting (Megachile rotundata) compared age-matched bees. The was induced addition canonical targets species upon unfolded protein (UPR) activation, suggesting that stress-induced regulation reactions common feature Therefore, study suggests can serve an important model biology broadly, although studies on will be required fully understand populations.

Language: Английский

Citations

2
Metabolic Syndrome: Lessons from Rodent and Drosophila Models DOI Creative Commons
Myroslava Vatashchuk, Maria M. Bayliak, Viktoria Hurza

et al.

BioMed Research International, Journal Year: 2022, Volume and Issue: 2022, P. 1 - 13

Published: June 22, 2022

Overweight and obesity are health conditions tightly related to a number of metabolic complications collectively called “metabolic syndrome” (MetS). Clinical diagnosis MetS includes the presence increased waist circumference or so-called abdominal obesity, reduced high density lipoprotein level, elevated blood pressure, glucose triacylglyceride levels. Different approaches, including diet-induced genetically induced animal models, have been developed study pathogenesis underlying mechanisms. Studies disturbances in fruit fly Drosophila mammalian models along with humans demonstrated that flies small like rats mice many similarities basic functions share molecular mechanisms which regulate these processes. In this paper, we describe diet-induced, chemically MetS. The advantages limitations rodent also analyzed.

Language: Английский

Citations

11
Identification, Synthesis, Conformation and Activity of an Insulin-like Peptide from a Sea Anemone DOI Creative Commons
Michela L. Mitchell, Mohammed Akhter Hossain, Feng Lin

et al.

Biomolecules, Journal Year: 2021, Volume and Issue: 11(12), P. 1785 - 1785

Published: Nov. 29, 2021

The role of insulin and insulin-like peptides (ILPs) in vertebrate animals is well studied. Numerous ILPs are also found invertebrates, although there uncertainty as to the function many these peptides. We have identified transcripts with similarity family tentacle transcriptomes sea anemone Oulactis sp. (Actiniaria: Actiniidae). translated showed that highly conserved A- B-chains among individuals this species, other Anthozoa. An ILP sequence (IlO1_i1) was synthesized using Fmoc solid-phase peptide synthesis individual chains, followed by regioselective disulfide bond formation intra-A two interchain bonds. Bioactivity studies IlO1_i1 were conducted on human growth factor receptors, voltage-gated potassium, sodium, calcium channels. did not bind or but weak activity against KV1.2, 1.3, 3.1, 11.1 (hERG) channels, NaV1.4 Further functional required determine anemone.

Language: Английский

Citations

14
Independent insulin signaling modulators govern hot avoidance under different feeding states DOI Creative Commons
Meng-Hsuan Chiang, Yu-Chun Lin, Sheng‐Fu Chen

et al.

PLoS Biology, Journal Year: 2023, Volume and Issue: 21(10), P. e3002332 - e3002332

Published: Oct. 17, 2023

Thermosensation is critical for the survival of animals. However, mechanisms through which nutritional status modulates thermosensation remain unclear. Herein, we showed that hungry Drosophila exhibit a strong hot avoidance behavior (HAB) compared to food-sated flies. We identified stimulus increases activity α'β' mushroom body neurons (MBns), with weak in sated state and state. Furthermore, MBn receives same level input from mALT projection via cholinergic transmission states. Differences between flies following heat stimuli are regulated by distinct insulin-like peptides (Dilps). Dilp2 secreted insulin-producing cells (IPCs) regulates HAB during satiety, whereas Dilp6 fat observed induces PI3K/AKT signaling, Ras/ERK signaling regulate different feeding conditions. Finally, 2 α'β'-related MB output (MBONs), MBON-α'3 MBON-β'1, necessary integrated information MBn. Our results demonstrate presence dual insulin modulation pathways MBn, important suitable behavioral responses thermoregulation under

Language: Английский

Citations

5
A kinase translocation reporter reveals real-time dynamics of ERK activity in Drosophila DOI Creative Commons
Alice C. Yuen, Anadika R. Prasad, Vilaiwan M. Fernandes

et al.

Biology Open, Journal Year: 2022, Volume and Issue: 11(5)

Published: May 15, 2022

ABSTRACT Extracellular signal-regulated kinase (ERK) lies downstream of a core signalling cascade that controls all aspects development and adult homeostasis. Recent developments have led to new tools image manipulate the pathway. However, visualising ERK activity in vivo with high temporal resolution remains challenge Drosophila. We adapted translocation reporter (KTR) for use Drosophila, which shuttles out nucleus when phosphorylated by ERK. show ERK-KTR faithfully reports endogenous developing tissues, it responds genetic perturbations upstream Using time-lapse imaging, we made two novel observations: firstly, sustained hyperactivation expression dominant-active epidermal growth factor receptor raised overall level but did not alter kinetics activity; secondly, direction migration retinal basal glia correlated their levels, suggesting an explanation heterogeneity observed fixed tissue. Our results KTR technology can be applied Drosophila monitor real-time suggest this modular tool further study other kinases. This article has associated First Person interview first author paper.

Language: Английский

Citations

8