Extracellular vesicles of Limosilactobacillus fermentum SLAM216 ameliorate skin symptoms of atopic dermatitis by regulating gut microbiome on serotonin metabolism

Gut Microbes, Journal Year: 2025, Volume and Issue: 17(1)

Published: March 3, 2025

Atopic dermatitis (AD) is a globally prevalent chronic inflammatory skin disorder. Its pathogenesis remains incompletely understood, resulting in considerable therapeutic challenges. Recent studies have highlighted the significance of interaction between AD and gut microbiome. In this study, we investigated effects probiotic-derived extracellular vesicles on AD. Initially, isolated characterized from Limosilactobacillus fermentum SLAM 216 (LF216EV) their composition through multi-omics analysis. Gene ontology (GO) pathway analysis classified LF216EV proteins into biological processes, molecular functions, cellular components. Importantly, specific abundance linoleic, oleic, palmitic, sebacic, stearic acids indicating upregulated fatty acid metabolism were observed by metabolomic Furthermore, featured lipid profiling including AcylGlcADG ceramide LF216EV. an atopic dermatitis-like cell model induced TNFα/IFNγ, significantly modulated expression immune regulatory genes (TSLP, TNFα, IL-6, IL-1β, MDC), its potential functionality dermatitis. alleviated AD-like phenotypes, such as redness, scaling/dryness, excoriation, DNCB. Histopathological revealed that decreased epidermal thickness mast infiltration dermis. administration reduced mouse scratching depression-related behaviors, with faster onset than classical treatment dexamethasone. quantitative real-time polymerase chain reaction (qRT-PCR) analysis, significant increase levels htrb2c, sert, tph-1, associated serotonin, LF216EV-treated group, along total serum serotonin levels. Gut microbiome group altered microbiota profile. Correlation genera Desulfovibrio differences intestinal metabolites, serotonin. Our findings demonstrate mitigates symptoms promoting synthesis modulation metabolome composition.

Language: Английский

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Defining Valid Chronic Stress Models for Depression With Female Rodents

Biological Psychiatry, Journal Year: 2021, Volume and Issue: 90(4), P. 226 - 235

Published: March 13, 2021

Language: Английский

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Effects of Social Isolation on the Development of Anxiety and Depression-Like Behavior in Model Experiments in Animals

Neuroscience and Behavioral Physiology, Journal Year: 2022, Volume and Issue: 52(5), P. 722 - 738

Published: June 1, 2022

Language: Английский

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Extended access self-administration of methamphetamine is associated with age- and sex-dependent differences in drug taking behavior and recognition memory in rats

Behavioural Brain Research, Journal Year: 2020, Volume and Issue: 390, P. 112659 - 112659

Published: May 8, 2020

Language: Английский

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Prolonged Social Isolation, Started Early in Life, Impairs Cognitive Abilities in Rats Depending on Sex

Brain Sciences, Journal Year: 2020, Volume and Issue: 10(11), P. 799 - 799

Published: Oct. 30, 2020

The chronic stress of social isolation is a valid predictor cognitive pathology. This study aimed to compare the effects long-term on female versus male Wistar rats' learning and memory. We hypothesized that prolonged stress, which starts early in life, would affect sex-dependent manner.Social started at edge mid-adolescence lasted 9 months. rat's abilities were assessed by habituation reactivity novelty open field (OF) test, spatial memory Morris water maze (MWM), conditioned passive avoidance (PA) reflex. Basal serum corticosterone levels using an enzyme-linked immunosorbent assay.Regardless housing conditions, females habituated OF under low illumination slower than males. Under bright light, single-housed rats showed hyporeactivity novelty. In MWM, all learned locate platform; however, first training day, females' speed was lower relative other groups. Four months later, post-reminder probe trial, reached area around platform site only males, regardless preferred target quadrant. Single-housed rats, irrespective sex, PA deficit. There more pronounced fear males females. both basal rat blood after did not differ from group-housed corresponding sex. Meanwhile, level higher conditions. weight adrenal glands increased females.Under isolation, compared with impairments MWM paradigm, findings specify their greater vulnerability isolation.

Language: Английский

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Risk factors for barbering in laboratory mice

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 3, 2025

Barbering is a common abnormal behavior in laboratory mice, where mice pluck their own fur and/or the or whiskers of cage mates. are concern for welfare and research quality, as well serving spontaneous model trichotillomania (a hair-pulling disorder humans). Causes prevention barbering poorly understood, although there evidence that both biological environmental factors play role its prevalence. Since initial work this area was done 20 years ago, mouse husbandry has changed dramatically. We provide an updated analysis risk based on point prevalence hair loss 2544 cages over one year (7007 mice). analyzed effects biological, environmental, known to be stressors mice. found certain barbering, such sex breeding status, have persisted despite changes housing. additionally identified differences genetic background, housing system, time year, "hotspot" effect showing spatial clustering barbering. Our findings can used increase understanding inform reduce

Language: Английский

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Voluntary wheel exercise improves learning and memory impairment caused by hippocampal Hb-α deficiency by reducing microglial activation and reversing synaptic damage

Brain Behavior and Immunity, Journal Year: 2025, Volume and Issue: 127, P. 81 - 95

Published: March 7, 2025

Language: Английский

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Pharmacological agents and injection stress, but not social isolation, alter cognitive judgement bias in the mouse touchscreen operant chamber

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 21, 2025

Cognitive judgement bias (CJB) refers to the interpretation of ambiguous stimuli in a negative (pessimistic) or positive (optimistic) way. Negative CJB is observed depression and anxiety, conditions that burden affected individuals their caregivers. Pre-clinical animal research key understanding developing therapies for affective anxiety disorders, so translationally relevant test would be useful addition existing pre-clinical rodent touchscreen battery. Here, task mouse operant chambers was adapted validated using pharmacological agents, bupropion tetrabenazine, known induce CJB, respectively. Further, shifts were following an injection procedure (handling intraperitoneal with saline solution), demonstrating sensitivity task. Interestingly, whereas social isolation has been linked changes state humans, 8 weeks single-housing mice did not alter but anxiety-like behaviour open field. Collectively, these results present assess mice, which will facilitate future explorations into underlying neurobiology potential treatment disorders.

Language: Английский

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Social reactivation of fear engrams enhances memory recall

Proceedings of the National Academy of Sciences, Journal Year: 2022, Volume and Issue: 119(12)

Published: March 14, 2022

For group-living animals, the social environment provides salient experience that can weaken or strengthen aspects of cognition such as memory recall. Although cellular substrates individually acquired fear memories in dentate gyrus (DG) and basolateral amygdala (BLA) have been well-studied recent work has revealed circuit mechanisms underlying encoding experience, processes by which interacts with an individual’s to alter recall remain unknown. Here we show stressful experiences enhance previously male but not female mice, buffering conspecifics’ distress blocks this enhancement. Activity-dependent tagging cells DG during learning these ensembles were endogenously reactivated males, even after extinction. These shown be functional components engrams, optogenetic stimulation active fear-conditioned naïve animals was sufficient drive fear-related behaviors. Taken together, our findings suggest reactivate preexisting engrams thereby discrete memories.

Language: Английский

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Stress Evaluation of Mouse Husbandry Environments for Improving Laboratory Animal Welfare

Animals, Journal Year: 2023, Volume and Issue: 13(2), P. 249 - 249

Published: Jan. 10, 2023

Animal welfare is recognized as essential for the coexistence of humans and animals. Considering increased demand interest in animal welfare, many methods improving are being devised, but which method reduces stress has not been scientifically verified. Therefore, reducing by providing a proper breeding environment environmental enrichment can be basis study. In this study, levels were assessed based on mouse-breeding environment. We considered that higher body weight lower corticosterone concentration, stress. According to results, animals individual ventilation cages determined have serum cortisol concentrations, while was when compared with isolated group or enrichment. The results provide appropriate guidelines laboratory welfare.

Language: Английский

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