Advances in insect physiology, Journal Year: 2021, Volume and Issue: unknown, P. 87 - 117
Published: Jan. 1, 2021
Language: Английский
Scientific Data, Journal Year: 2025, Volume and Issue: 12(1)
Published: Feb. 19, 2025
Abstract The Colorado potato beetle (CPB) is a major pest of crops, known for its remarkable ability to develop resistance more than 50 pesticides. For decades, CPB has served as model species studying insecticide resistance, insect physiology, diapause, reproduction, and evolution. However, research progress on been hindered by the lack comprehensive genomic transcriptomic resources. Here, leveraging recently established chromosome-level genome assembly, we constructed gene expression atlas using data from 61 samples representing organs developmental stages. By integrating short- long-read sequencing technologies, enhanced annotation identified 6,623 additional genes that were previously undetected. Furthermore, developed web portal facilitate search visualization atlas, providing an accessible resource community. offers valuable tools will accelerate future in control biology.
Language: Английский
Citations
0
Frontiers in Marine Science, Journal Year: 2022, Volume and Issue: 9
Published: Feb. 28, 2022
Molting behavior in insects is controlled by the ecdysis triggering hormone (ETH), eclosion (EH) and crustacean cardioactive peptide (CCAP). At present, regulation of molting crustaceans remains unclear. Here, we studied roles ETH , EH CCAP molt crab, Scylla paramamosain from their expression pattern vivo assays. The results showed that transcripts were mainly localized thoracic ganglia fluctuated periodically with cycle. When or was knockdown at early premolt stage (D0), crabs interrupted all animals died late (D2). While gene knock-down, most dead before D2. Injection synthetic for rescued - -gene knock-down separately. However, none peptides could rescue dsEH -injected crabs. D0 stage, down-regulated transcriptions ; while up-regulated when knockdown. D2 levels reduced injection but increased same dose dsETH as stage. Co-injection ds Results play essential different mud crab. In summary, result this study contributes to discovery molecular mechanisms between Insecta Crustacea may provide insight develop fishery drugs helps aquacultured successfully.
Language: Английский
Citations
10
Pesticide Biochemistry and Physiology, Journal Year: 2021, Volume and Issue: 182, P. 105029 - 105029
Published: Dec. 30, 2021
Language: Английский
Citations
14
Frontiers in Endocrinology, Journal Year: 2022, Volume and Issue: 13
Published: July 28, 2022
Ecdysis triggering hormone (ETH) plays an important role in molting, reproduction, and courtship behavior insects. To investigate the potential downstream pathways genes of ETH Scylla paramamosain , RNA interference (RNAi) was conducted on crabs at early (D0) late (D2) premolt substages, transcriptome profiles each group were compared by sequencing. Real-time quantitative polymerase chain reaction (RT-qPCR) semiquantitative (RT-PCR) results showed a significant knockdown D0 stage, whereas increase shown conversely D2 substage after injection dsETH . A total 242,979 transcripts assembled, 44,012 unigenes identified. Transcriptomic comparison between substages 2,683 differentially expressed (DEGs); these enriched ribosome related to transcription factor complex cell part. Twenty DEGs identified -injected dsGFP substage; involved carbohydrate metabolism, one carbon pool folate, chitin binding. Twenty-six calcium channel inhibitor activity, fat digestion absorption, cardiac muscle contraction. RT-qPCR verified differential expression selected genes. In conclusion, are more active preparing macromolecular that is needed for molting. Moreover, has roles binding substage, while turns be contraction facilitate occurrence The provide valuable insight into regulation crustacean
Language: Английский
Citations
7
International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(22), P. 13713 - 13713
Published: Nov. 8, 2022
Cotton is the most important crop for fiber production worldwide. However, cotton boll weevil (CBW) an insect pest that causes significant economic losses in infested areas. Current control methods are costly, inefficient, and environmentally hazardous. Herein, we generated transgenic lines expressing double-stranded RNA (dsRNA) molecules to trigger interference-mediated gene silencing CBW. Thus, targeted three essential genes coding chitin synthase 2, vitellogenin, ecdysis-triggering hormone receptor. The stability of expressed dsRNAs was improved by designing a structured based on viroid genome architecture. We transformed embryos inserting promoter-driven expression cassette overexpressed dsRNA into flower buds. plants were characterized, positive PCR events detected with average heritability 80%. Expression confirmed floral buds RT-qPCR, T1 plant generation challenged fertilized CBW females. After 30 days, data showed high mortality (around 70%) oviposited yolks. In adult insects fed lines, II vitellogenin reduced larvae adults, respectively. Developmental delays abnormalities also observed these individuals. Our remark potential viroid-structured
Language: Английский
Citations
7
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: March 31, 2024
Abstract The Colorado potato beetle (CPB) is a major pest of crops that has evolved resistance to more than 50 pesticides. For decades, CPB been model species for research on insecticide resistance, insect physiology, diapause, reproduction and evolution. Yet, the progress in constrained by lack comprehensive genomic transcriptomic information. Here, building recently established chromosome-level genome assembly, we built gene expression atlas using transcriptomes 61 samples representing organs developmental stages. By both short long reads, improved annotation identified 6,658 genes were missed previous annotations. We then web portal allowing search visualization community. provides useful tools data, which will accelerate future control biology fields.
Language: Английский
Citations
1
Pest Management Science, Journal Year: 2022, Volume and Issue: 78(9), P. 3849 - 3858
Published: Feb. 1, 2022
RNA interference (RNAi) is a breakthrough technology in pest control. It highly efficient to Coleopteran pests such as the Colorado potato beetle Leptinotarsa decemlineata, serious defoliator mainly attacking potatoes worldwide. The first step for effective control by RNAi development of and reliable target genes.Our results revealed that continuous ingestion dsLdRan 3 days successfully silenced gene, inhibited larval growth killed 100% L. decemlineata larvae. When bioassay began at second-, third/fourth-instar stages, lethality occurred fourth instar prepupal respectively. Importantly, consumption newly-emerged males females effectively knocked down transcript, reduced fresh weights caused within week. LdRan possessed underdeveloped ovaries.Considering larvae, adults eggs are simultaneously sited on plants, bacterially-expressed potential RNAi-based strategy managing field. © 2022 Society Chemical Industry.
Language: Английский
Citations
6
Pesticide Biochemistry and Physiology, Journal Year: 2021, Volume and Issue: 175, P. 104838 - 104838
Published: April 3, 2021
Language: Английский
Citations
5
Insect Science, Journal Year: 2022, Volume and Issue: 29(5), P. 1309 - 1317
Published: Jan. 12, 2022
Infections by mosquito-borne diseases represent one of the leading causes death in third world countries. The rapid progression resistance to conventional insecticide a significant threat highly efficient preventive methods currently place. Insect neuropeptidergic system offers potential targets control insect vectors. essential roles neuropeptide ecdysis triggering hormone (ETH) development and reproduction led us attempt understanding fundamentals biochemical interaction between ETH its receptor African malaria mosquito Anopheles gambiae. One two peptides (AgETH1), small peptide with 17 amino acid residues (SESPGFFIKLSKSVPRI-NH2 ), was studied elucidate molecular structure. N-termini deletions mutations conserved acids ligand revealed critical for activation. solution structure AgETH1 using 2D 1 H-1 H nuclear magnetic resonance (NMR) spectroscopy overhauser effect (NOE) derived constraints short alpha helix 3S 11S. NMR will be assistance designing new class insecticidal compounds that acts on AgETH aiming silico docking studies.
Language: Английский
Citations
3
Comparative Biochemistry and Physiology Part A Molecular & Integrative Physiology, Journal Year: 2024, Volume and Issue: 297, P. 111692 - 111692
Published: July 6, 2024
Language: Английский
Citations
0