Combined with dynamic serum proteomics and clinical follow-up to screen the serum proteins to promote the healing of diabetic foot ulcer DOI Creative Commons
Fengying Zhao, Liangxiao Xie,

Zhiyan Weng

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: June 9, 2023

Abstract ObjectiveNon-healing diabetic foot ulcers are a leading cause of disability and death in patients, which often results lower limb amputation. This study aimed to investigate the impact biomarkers on healing by utilizing dynamic serum proteomics skin proteomic analysis, combined with clinical case follow-up studies.MethodsTo analyze changes four groups, age-matched normal subjects, pre-treatment ulcer healed patients were selected. The differential proteins screened conjunction proteomics. In this study, total 80 enrolled monitored for 3–6 months during treatment. To verify significance proteins, (240 patients) healthy controls (160 included as controls.ResultsDynamic trend showed that level negative regulatory related endothelial cell migration, angiogenesis, vascular development was significantly decreased after treatment ulcer. also found there is an opposite protein biological processes, mainly enriched activation cascade, immunoglobulin production, complement activation. researchers identified core APOA1, LPA, APOA2 through convergence screening. Clinical cases further validated APOA1 levels correlated disease severity.ConclusionsBased our studies, bioinformatic analysis suggests plays critical role linking coagulation, inflammation, wound repair, making it key promotes ulcers.

Language: Английский

Recent advances in molecular mechanisms of skin wound healing and its treatments DOI Creative Commons
Abdullah Al Mamun, Chuxiao Shao, Peiwu Geng

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: May 21, 2024

The skin, being a multifaceted organ, performs pivotal function in the complicated wound-healing procedure, which encompasses triggering of several cellular entities and signaling cascades. Aberrations typical healing process wounds may result atypical scar development establishment persistent condition, rendering patients more vulnerable to infections. Chronic burns have detrimental effect on overall quality life patients, resulting higher levels physical discomfort socio-economic complexities. occurrence frequency prolonged are rise as aging people, hence contributing escalated expenditures within healthcare system. clinical evaluation treatment chronic continue pose challenges despite advancement different therapeutic approaches. This is mainly owing duration intricate processes involved wound healing. Many conventional methods, such administration growth factors, use dressings, application skin grafts, used ease across diverse types. Nevertheless, these approaches only be practical for some wounds, highlighting need advance alternative modalities. Novel care technologies, nanotherapeutics, stem cell treatment, 3D bioprinting, aim improve efficacy, prioritize regeneration, minimize adverse effects. review provides an updated overview recent advancements management using innovative

Language: Английский

Citations

29
Innovative Bio‐based Hydrogel Microspheres Micro‐Cage for Neutrophil Extracellular Traps Scavenging in Diabetic Wound Healing DOI Creative Commons

Yongqiang Xiao,

Tao Ding, Fang He

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 11(21)

Published: April 6, 2024

Abstract Neutrophil extracellular traps (NETs) seriously impede diabetic wound healing. The disruption or scavenging of NETs using deoxyribonuclease (DNase) cationic nanoparticles has been limited by liberating trapped bacteria, short half‐life, potential cytotoxicity. In this study, a positive correlation between the level in exudation and severity inflammation patients is established. Novel bio‐based hydrogel microspheres ‘micro‐cage’, termed mPDA‐PEI@GelMA, engineered integrating methylacrylyl gelatin (GelMA) with polyethyleneimine (PEI)‐functionalized mesoporous polydopamine (mPDA). This unique ‘micro‐cage’ construct designed to non‐contact scavenge surface, minimizing biological toxicity ensuring high biosafety. are introduced into along exudation, mPDA‐PEI immobilizes them inside through strong binding affinity cfDNA web structure. findings demonstrate that mPDA‐PEI@GelMA effectively mitigates pro‐inflammatory responses associated wounds both vivo vitro. work introduces novel nanoparticle strategy enhance healing processes, benefits clinical applications.

Language: Английский

Citations

13
Neutrophils extracellular traps and ferroptosis in diabetic wounds DOI Creative Commons
Yumeng Huang, Youjun Ding,

Beizhi Wang

et al.

International Wound Journal, Journal Year: 2023, Volume and Issue: 20(9), P. 3840 - 3854

Published: May 17, 2023

Abstract Wound healing is an extremely complex process involving multiple levels of cells and tissues. It mainly completed through four stages: haemostasis, inflammation, proliferation, remodelling. When any one these stages impaired, it may lead to delayed or even transformation into chronic refractory wounds. Diabetes a kind common metabolic disease that affects approximately 500 million people worldwide, 25% whom develop skin ulcers break down repeatedly are difficult heal, making growing public health problem. Neutrophils extracellular traps ferroptosis new types programmed cell death identified in recent years have been found interact with diabetic In this paper, the normal wound interfering factors were outlined. The mechanism two kinds was also described, interaction between different wounds discussed.

Language: Английский

Citations

18
The Role of p53 in Regulating Chronic Inflammation and PANoptosis in Diabetic Wounds DOI Creative Commons
Wenjie He, Ming Tang,

Rifang Gu

et al.

Aging and Disease, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Diabetic wounds represent a formidable challenge in the clinical management of diabetes mellitus, markedly diminishing patient's quality life. These arise from multifaceted etiology, with pathophysiological underpinnings remaining elusive and complex. Diabetes precipitates neuropathies vasculopathies lower extremities, culminating infections, ulcerations, extensive tissue damage. The hallmarks non-healing diabetic include senescence, persistent inflammation, heightened apoptosis, attenuated cellular proliferation. TP53 gene, pivotal tumor suppressor frequently silenced human malignancies, orchestrates proliferation, DNA repair, apoptosis. While p53 is integral cell cycle regulation, its role initial repair appears to be deleterious. In typical cutaneous wounds, levels transiently dip, swiftly reverting baseline. Yet protracted activation impedes healing via two distinct pathways: i) activating p53-p21-Retinoblastoma (RB) axis, which halts cycle, ii) upregulating cGAS-STING nuclear factor-kappaB (NF-κB) cascades, instigating ferroptosis pyroptosis. Furthermore, intersects various metabolic pathways, including glycolysis, gluconeogenesis, oxidative phosphorylation, autophagy. may drive reprogramming, thus potentially derailing macrophage polarization. This review synthesizes case studies investigating therapeutic modulation care. summation, modulates chronic inflammation aging within implicated novel death modality, encompassing pyroptosis, hinders reparative process.

Language: Английский

Citations

5
A comprehensive exploration of hydrogel applications in multi-stage skin wound healing DOI
Yongping Lu, Yuemin Wang, Jie Wang

et al.

Biomaterials Science, Journal Year: 2024, Volume and Issue: 12(15), P. 3745 - 3764

Published: Jan. 1, 2024

Hydrogels, as an emerging biomaterial, have found extensive use in the healing of wounds due to their distinctive physicochemical structure and functional properties. Moreover, hydrogels can be made match a range therapeutic requirements for materials used wound through specific modifications. This review provides step-by-step explanation processes involved cutaneous healing, including hemostasis, inflammation, proliferation, reconstitution, along with investigation factors that impact these processes. Furthermore, thorough analysis is conducted on various stages process at which are implemented, anti-infection measures, encouraging regeneration, scar reduction, monitoring. Next, latest progress multifunctional methods achieve functions discussed depth categorized elucidation. Finally, perspectives challenges associated clinical applications discussed.

Language: Английский

Citations

5
Pathological Insights into Cell Death Pathways in Diabetic Wound Healing DOI
Kannan Harithpriya,

Srinivasan Kaussikaa,

Srikanth Kavyashree

et al.

Pathology - Research and Practice, Journal Year: 2024, Volume and Issue: 264, P. 155715 - 155715

Published: Nov. 7, 2024

Language: Английский

Citations

4
T-2 toxin triggers immunotoxic effects in goats by inducing ferroptosis and neutrophil extracellular traps DOI
Jing Huang,

Kaifeng He,

Xin Guo

et al.

Toxicology and Applied Pharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 117232 - 117232

Published: Jan. 1, 2025

Language: Английский

Citations

0
The interplay between the myeloperoxidase-hypochlorous acid system, heme oxygenase, and free iron in inflammatory diseases DOI
Monika Biernat, Olivia G. Camp,

Daniel Moussa

et al.

Journal of Inorganic Biochemistry, Journal Year: 2025, Volume and Issue: 270, P. 112927 - 112927

Published: April 17, 2025

Language: Английский

Citations

0
The mechanism of action of GLUT1 in promoting NETs-mediated impairment of macrophage phenotypic switching based on macrophage-fibroblast interplay DOI Creative Commons
Wenqiang Li, Shijie Li, Weijing Sun

et al.

Cytokine, Journal Year: 2025, Volume and Issue: 191, P. 156946 - 156946

Published: April 23, 2025

Language: Английский

Citations

0
Theory-screened Prussian blue analogues-based nanozymes for promoting diabetic wound healing via ferroptosis inhibition DOI Creative Commons
Qingrong Dong, Xiaomei Shen, Fang Ge

et al.

Materials Today Bio, Journal Year: 2025, Volume and Issue: unknown, P. 101839 - 101839

Published: May 1, 2025

Language: Английский

Citations

0