Biomaterial-based mechanical regulation facilitates scarless wound healing with functional skin appendage regeneration

Military Medical Research, Journal Year: 2024, Volume and Issue: 11(1)

Published: Feb. 18, 2024

Abstract Scar formation resulting from burns or severe trauma can significantly compromise the structural integrity of skin and lead to permanent loss appendages, ultimately impairing its normal physiological function. Accumulating evidence underscores potential targeted modulation mechanical cues enhance regeneration, promoting scarless repair by influencing extracellular microenvironment driving phenotypic transitions. The field appendage regeneration has witnessed remarkable advancements in utilization biomaterials with distinct physical properties. However, a comprehensive understanding underlying mechanisms remains somewhat elusive, limiting broader application these innovations. In this review, we present two promising biomaterial-based approaches aimed at bolstering regenerative capacity compromised skin. first approach involves leveraging specific biophysical properties create an optimal environment that supports cellular activities essential for regeneration. second centers on harnessing forces exerted plasticity, facilitating efficient reprogramming and, consequently, appendages. summary, manipulation using strategies holds significant promise as supplementary achieving wound healing, coupled restoration multiple functions.

Language: Английский

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Novel Factors Regulating Proliferation, Migration, and Differentiation of Fibroblasts, Keratinocytes, and Vascular Smooth Muscle Cells during Wound Healing

Biomedicines, Journal Year: 2024, Volume and Issue: 12(9), P. 1939 - 1939

Published: Aug. 23, 2024

Chronic diabetic foot ulcers (DFUs) are a significant complication of diabetes mellitus, often leading to amputation, increased morbidity, and substantial financial burden. Even with the advancements in treatment DFU, risk amputation still exists, this occurs due presence gangrene osteomyelitis. Nonhealing chronic DFU is decreased angiogenesis, granulation tissue formation, extracellular matrix remodeling persistent inflammation. During wound healing, proliferation migration fibroblasts, smooth muscle cells, keratinocytes play critical role (ECM) remodeling, epithelialization. The molecular factors regulating migration, proliferation, differentiation these cells scarcely discussed literature. literature review identifies key influencing keratinocytes, vascular (VSMCs), which healing. This followed by discussion on various novel but not context healing; however, they may role. Using network analysis, we examined interactions between factors, findings suggest that identified promoting during healing or However, warrant further investigation establish their alone synergistically.

Language: Английский

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Ear pinna growth and differentiation is conserved in murids and requires BMP signaling for chondrocyte proliferation

Development, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 23, 2025

Despite being a major target of reconstructive surgery, development the ear pinna remains poorly studied. Here we provide cellular characterization late gestational and postnatal in two rodents investigate role BMP5 expansion differentiation auricular elastic cartilage. We find that is largely conserved between Mus musculus highly regenerative Acomys dimidiatus. The pattern pre-cartilaginous cells established early development. These are specified into chondroblasts prior to unfolding then undergo extensive proliferation maturation. cartilage, connective tissue fibroblasts, dermal papilla sheath cells, adipocytes adult derived from cranial neural crest. Cellular analysis using naturally occurring short mouse mutant shows loss does not prevent specification but impairs their proliferation. Finally, chondroblast impaired mid-distal these mutants. Together data establish developmental basis for tissues.

Language: Английский

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Direct fibroblast reprogramming: an emerging strategy for treating organic fibrosis

Journal of Translational Medicine, Journal Year: 2025, Volume and Issue: 23(1)

Published: Feb. 27, 2025

Direct reprogramming has garnered considerable attention due to its capacity directly convert differentiated cells into desired cells. Fibroblasts are frequently employed in studies their abundance and accessibility. However, they also the key drivers progression of fibrosis, a pathological condition characterized by excessive extracellular matrix deposition tissue scarring. Furthermore, initial stage typically involves deactivating fibrotic pathways. Hence, direct offers valuable method regenerate target for repair while simultaneously reducing tendencies. Understanding link between fibrosis could help develop effective strategies treat damaged with potential risk fibrosis. This review summarizes advances reveals anti-fibrosis effects various organs such as heart, liver, skin. we dissect mechanisms influenced molecules including TGF-β signaling, mechanical inflammation epigenetic modifiers, metabolic regulators. Innovative methods fibroblast like small molecules, CRISPRa, modified mRNA, challenges cellular heterogeneity senescence faced vivo reprogramming, discussed.

Language: Английский

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Novel prospects for scarless wound healing: The roles of myofibroblasts and adipocytes

Journal of Cellular and Molecular Medicine, Journal Year: 2022, Volume and Issue: 26(20), P. 5113 - 5121

Published: Sept. 15, 2022

Abstract Disturbances or defects in the process of wound repair can disrupt delicate balance cells and molecules necessary for complete healing, thus leading to chronic wounds fibrotic scars. Myofibroblasts are one most important involved scars, reprogramming provides a potential avenue increase myofibroblast clearance. Although myofibroblasts have long been recognized as terminally differentiated cells, recent studies shown that capacity be reprogrammed into adipocytes. This review intends summarize We will discuss lineage tracing, well known mechanisms underlying adipocyte regeneration from myofibroblasts. In addition, we investigated different changes gene expression, transcriptional regulators, signalling pathways epigenetic regulators during skin healing. future, healing better understood appreciated, which may provide new ideas treatment scarless

Language: Английский

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Effects of exosomes from human dental pulp stem cells on the biological behavior of human fibroblasts

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 7, 2025

The aim of this study was to investigate the effect dental pulp stem cell-derived exosomes (DPSCs-Exos) on biological behaviour fibroblasts, particularly keloid fibroblasts (KFs) and normal skin (NFs), with a view providing new insights into cellular regenerative medicine. We obtained DPSCs-Exos by ultracentrifugation co-cultured it KFs NFs. detected its cell proliferation using CCK-8 assay; migration ability scratch Transwell assays; extracellular matrix synthesis hydroxyproline content expression levels genes associated fibrosis PCR proteins related in cells Western Blot method. able be taken up after addition culture medium affected behavior NFs KFs. promoted NFs, inhibited In addition, inhibit fibrosis-related This highlights role regulating for future applications field cell-free

Language: Английский

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Effects of photobiomodulation on adipocytic infiltration in sites of skin healing: in vivo experimental study

Lasers in Medical Science, Journal Year: 2025, Volume and Issue: 40(1)

Published: March 20, 2025

Language: Английский

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SHP099-containing multi-targeting hydrogel promotes rapid skin reconstruction through modulating a variety of cells

Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 13

Published: April 7, 2025

Adult wound scarring result in functional skin deficits. However, the development of effective measures to modulate entire healing encourage function reconstruction is still a clinical challenge, as multiple cells are involved hierarchically. Hydrogel scaffolds with long-lasting local release provide new insights into relevance healing. Herein, multi-targeting hydrogel loaded SHP099 (Gel-SHP) designed during repair. Our results show that Gel-SHP promotes rapid by modulating macrophages inflammatory stage, fibroblasts regeneration stage and smooth muscle remodelling stage. could increase M2 macrophage differentiation remodel dermal shell hair follicles through situ release. Moreover, may myofibroblasts promote contraction SHP099-scaffold synergistic interactions. design hydrogels for tissue applications. promising tool clues research paradigms future studies understanding process formation.

Language: Английский

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A hypothesis on the combination of platelet concentrates and adipocytes promoting wound healing through a bidirectional regulatory mechanism

Medical Hypotheses, Journal Year: 2025, Volume and Issue: unknown, P. 111648 - 111648

Published: May 1, 2025

Language: Английский

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Spiny mice (Acomys) have evolved cellular features to support regenerative healing

Annals of the New York Academy of Sciences, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 13, 2025

Spiny mice (Acomys spp.) are warm-blooded (homeothermic) vertebrates whose ability to restore missing tissue through regenerative healing has coincided with the evolution of unique cellular and physiological adaptations across different types. This review seeks explore how these bizarre rodents deploy or altered injury response mechanisms either enhance repair fully regenerate excised compared closely related, scar-forming mammals. First, we examine overall trends in Acomys tissues, including stress response, activate maintain cell cycle progression, expression certain features reproductive adults that normally associated embryos. Second, focus on specific types exhibit precisely regulated proliferation tissue. While utilize many same involved scar formation, cells divergent activation profiles during healing. Considered together, current lines evidence support sustained deployment proregenerative pathways conjunction transient fibrotic facilitate regeneration improve Acomys.

Language: Английский

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