Research Square (Research Square),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Oct. 11, 2023
Abstract
Diabetic
ulcer
healing
is
affected
by
persistent
hyperglycemia,
and
the
current
treatment
methods
are
extremely
limited.
We
aim
to
take
advantages
of
stem
cell
therapy
try
use
periodontal
ligament
cells
that
have
not
yet
become
therapeutic
repair
diabetic
wounds,
so
as
provide
theoretical
basis
for
its
application
in
clinical
practice.
Methods:
The
effect
AGEs
on
myofibroblast
differentiation
ability
hPDLSCs
was
investigated
RT-qPCR
WBto
detect
gene
protein
expressions
α-SMA
COLIA1.
HE
staining
Masson
were
used
observe
wound
STZ-induced
mice,
immunohistochemical
expression
CD31.
Immunofluorescence
(IF)
CD206
iNOS
tissue
area.hPDLSCs
can
spontaneously
differentiate
into
myoblasts
during
culture.
In
α-MEM
medium,
at
25μg/mL
AGEs,
COLIA1
seventh
passage
(P7)
significantly
decreased,
increased
(P
<
0.05).
hydrogel
injection
heal
wounds
C57
it
found
rate
group
65.247%
7th
day
while
model
48.938%.
much
higher
than
group.
Conclusion:
skin
mice
differentiating
myofibroblasts
resisting
damage
which
expected
be
diagnosis
treatment.
Military Medical Research,
Journal Year:
2024,
Volume and Issue:
11(1)
Published: Feb. 18, 2024
Abstract
Scar
formation
resulting
from
burns
or
severe
trauma
can
significantly
compromise
the
structural
integrity
of
skin
and
lead
to
permanent
loss
appendages,
ultimately
impairing
its
normal
physiological
function.
Accumulating
evidence
underscores
potential
targeted
modulation
mechanical
cues
enhance
regeneration,
promoting
scarless
repair
by
influencing
extracellular
microenvironment
driving
phenotypic
transitions.
The
field
appendage
regeneration
has
witnessed
remarkable
advancements
in
utilization
biomaterials
with
distinct
physical
properties.
However,
a
comprehensive
understanding
underlying
mechanisms
remains
somewhat
elusive,
limiting
broader
application
these
innovations.
In
this
review,
we
present
two
promising
biomaterial-based
approaches
aimed
at
bolstering
regenerative
capacity
compromised
skin.
first
approach
involves
leveraging
specific
biophysical
properties
create
an
optimal
environment
that
supports
cellular
activities
essential
for
regeneration.
second
centers
on
harnessing
forces
exerted
plasticity,
facilitating
efficient
reprogramming
and,
consequently,
appendages.
summary,
manipulation
using
strategies
holds
significant
promise
as
supplementary
achieving
wound
healing,
coupled
restoration
multiple
functions.
Biomedicines,
Journal Year:
2024,
Volume and Issue:
12(9), P. 1939 - 1939
Published: Aug. 23, 2024
Chronic
diabetic
foot
ulcers
(DFUs)
are
a
significant
complication
of
diabetes
mellitus,
often
leading
to
amputation,
increased
morbidity,
and
substantial
financial
burden.
Even
with
the
advancements
in
treatment
DFU,
risk
amputation
still
exists,
this
occurs
due
presence
gangrene
osteomyelitis.
Nonhealing
chronic
DFU
is
decreased
angiogenesis,
granulation
tissue
formation,
extracellular
matrix
remodeling
persistent
inflammation.
During
wound
healing,
proliferation
migration
fibroblasts,
smooth
muscle
cells,
keratinocytes
play
critical
role
(ECM)
remodeling,
epithelialization.
The
molecular
factors
regulating
migration,
proliferation,
differentiation
these
cells
scarcely
discussed
literature.
literature
review
identifies
key
influencing
keratinocytes,
vascular
(VSMCs),
which
healing.
This
followed
by
discussion
on
various
novel
but
not
context
healing;
however,
they
may
role.
Using
network
analysis,
we
examined
interactions
between
factors,
findings
suggest
that
identified
promoting
during
healing
or
However,
warrant
further
investigation
establish
their
alone
synergistically.
Development,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 23, 2025
Despite
being
a
major
target
of
reconstructive
surgery,
development
the
ear
pinna
remains
poorly
studied.
Here
we
provide
cellular
characterization
late
gestational
and
postnatal
in
two
rodents
investigate
role
BMP5
expansion
differentiation
auricular
elastic
cartilage.
We
find
that
is
largely
conserved
between
Mus
musculus
highly
regenerative
Acomys
dimidiatus.
The
pattern
pre-cartilaginous
cells
established
early
development.
These
are
specified
into
chondroblasts
prior
to
unfolding
then
undergo
extensive
proliferation
maturation.
cartilage,
connective
tissue
fibroblasts,
dermal
papilla
sheath
cells,
adipocytes
adult
derived
from
cranial
neural
crest.
Cellular
analysis
using
naturally
occurring
short
mouse
mutant
shows
loss
does
not
prevent
specification
but
impairs
their
proliferation.
Finally,
chondroblast
impaired
mid-distal
these
mutants.
Together
data
establish
developmental
basis
for
tissues.
Journal of Translational Medicine,
Journal Year:
2025,
Volume and Issue:
23(1)
Published: Feb. 27, 2025
Direct
reprogramming
has
garnered
considerable
attention
due
to
its
capacity
directly
convert
differentiated
cells
into
desired
cells.
Fibroblasts
are
frequently
employed
in
studies
their
abundance
and
accessibility.
However,
they
also
the
key
drivers
progression
of
fibrosis,
a
pathological
condition
characterized
by
excessive
extracellular
matrix
deposition
tissue
scarring.
Furthermore,
initial
stage
typically
involves
deactivating
fibrotic
pathways.
Hence,
direct
offers
valuable
method
regenerate
target
for
repair
while
simultaneously
reducing
tendencies.
Understanding
link
between
fibrosis
could
help
develop
effective
strategies
treat
damaged
with
potential
risk
fibrosis.
This
review
summarizes
advances
reveals
anti-fibrosis
effects
various
organs
such
as
heart,
liver,
skin.
we
dissect
mechanisms
influenced
molecules
including
TGF-β
signaling,
mechanical
inflammation
epigenetic
modifiers,
metabolic
regulators.
Innovative
methods
fibroblast
like
small
molecules,
CRISPRa,
modified
mRNA,
challenges
cellular
heterogeneity
senescence
faced
vivo
reprogramming,
discussed.
Journal of Cellular and Molecular Medicine,
Journal Year:
2022,
Volume and Issue:
26(20), P. 5113 - 5121
Published: Sept. 15, 2022
Abstract
Disturbances
or
defects
in
the
process
of
wound
repair
can
disrupt
delicate
balance
cells
and
molecules
necessary
for
complete
healing,
thus
leading
to
chronic
wounds
fibrotic
scars.
Myofibroblasts
are
one
most
important
involved
scars,
reprogramming
provides
a
potential
avenue
increase
myofibroblast
clearance.
Although
myofibroblasts
have
long
been
recognized
as
terminally
differentiated
cells,
recent
studies
shown
that
capacity
be
reprogrammed
into
adipocytes.
This
review
intends
summarize
We
will
discuss
lineage
tracing,
well
known
mechanisms
underlying
adipocyte
regeneration
from
myofibroblasts.
In
addition,
we
investigated
different
changes
gene
expression,
transcriptional
regulators,
signalling
pathways
epigenetic
regulators
during
skin
healing.
future,
healing
better
understood
appreciated,
which
may
provide
new
ideas
treatment
scarless
Scientific Reports,
Journal Year:
2025,
Volume and Issue:
15(1)
Published: Jan. 7, 2025
The
aim
of
this
study
was
to
investigate
the
effect
dental
pulp
stem
cell-derived
exosomes
(DPSCs-Exos)
on
biological
behaviour
fibroblasts,
particularly
keloid
fibroblasts
(KFs)
and
normal
skin
(NFs),
with
a
view
providing
new
insights
into
cellular
regenerative
medicine.
We
obtained
DPSCs-Exos
by
ultracentrifugation
co-cultured
it
KFs
NFs.
detected
its
cell
proliferation
using
CCK-8
assay;
migration
ability
scratch
Transwell
assays;
extracellular
matrix
synthesis
hydroxyproline
content
expression
levels
genes
associated
fibrosis
PCR
proteins
related
in
cells
Western
Blot
method.
able
be
taken
up
after
addition
culture
medium
affected
behavior
NFs
KFs.
promoted
NFs,
inhibited
In
addition,
inhibit
fibrosis-related
This
highlights
role
regulating
for
future
applications
field
cell-free
Frontiers in Bioengineering and Biotechnology,
Journal Year:
2025,
Volume and Issue:
13
Published: April 7, 2025
Adult
wound
scarring
result
in
functional
skin
deficits.
However,
the
development
of
effective
measures
to
modulate
entire
healing
encourage
function
reconstruction
is
still
a
clinical
challenge,
as
multiple
cells
are
involved
hierarchically.
Hydrogel
scaffolds
with
long-lasting
local
release
provide
new
insights
into
relevance
healing.
Herein,
multi-targeting
hydrogel
loaded
SHP099
(Gel-SHP)
designed
during
repair.
Our
results
show
that
Gel-SHP
promotes
rapid
by
modulating
macrophages
inflammatory
stage,
fibroblasts
regeneration
stage
and
smooth
muscle
remodelling
stage.
could
increase
M2
macrophage
differentiation
remodel
dermal
shell
hair
follicles
through
situ
release.
Moreover,
may
myofibroblasts
promote
contraction
SHP099-scaffold
synergistic
interactions.
design
hydrogels
for
tissue
applications.
promising
tool
clues
research
paradigms
future
studies
understanding
process
formation.
Annals of the New York Academy of Sciences,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 13, 2025
Spiny
mice
(Acomys
spp.)
are
warm-blooded
(homeothermic)
vertebrates
whose
ability
to
restore
missing
tissue
through
regenerative
healing
has
coincided
with
the
evolution
of
unique
cellular
and
physiological
adaptations
across
different
types.
This
review
seeks
explore
how
these
bizarre
rodents
deploy
or
altered
injury
response
mechanisms
either
enhance
repair
fully
regenerate
excised
compared
closely
related,
scar-forming
mammals.
First,
we
examine
overall
trends
in
Acomys
tissues,
including
stress
response,
activate
maintain
cell
cycle
progression,
expression
certain
features
reproductive
adults
that
normally
associated
embryos.
Second,
focus
on
specific
types
exhibit
precisely
regulated
proliferation
tissue.
While
utilize
many
same
involved
scar
formation,
cells
divergent
activation
profiles
during
healing.
Considered
together,
current
lines
evidence
support
sustained
deployment
proregenerative
pathways
conjunction
transient
fibrotic
facilitate
regeneration
improve
Acomys.