Halotolerant Microorganism-Based Soil Conditioner Application Improved the Soil Properties, Yield, Quality and Starch Characteristics of Hybrid Rice under Higher Saline Conditions

Plants, Journal Year: 2024, Volume and Issue: 13(16), P. 2325 - 2325

Published: Aug. 21, 2024

Soil salinity represents a significant factor affecting agricultural productivity and crop quality. The present study was conducted to investigate the effects of soil conditioner (SC) comprising halotolerant microorganisms on fertility, yield, rice quality, physicochemical structural properties starch in hybrid under saline conditions. experimental treatments were composed two high-quality varieties, i.e., ‘Y Liangyou 957’ (YLY957) Jing 534 (JLY534), amendment treatments, application SC at control levels 2250 kg hm−2, or ‘CK SC’, respectively. subjected mixture fresh sea water (EC 11 dS/m). results demonstrated that significantly enhanced yield salt stress conditions owing an increase number grains per panicle. Furthermore, found be effective improving organic matter nutrient content. resulted improvement antioxidant defense, higher leaf SPAD values, greater biomass, as well translocation photo-assimilates heading stage. not only improved milling appearance quality but also taste value by increasing amylose reducing protein decreased indentations surfaces granules cracks edges granules. varieties exhibited excellent pasting properties, characterized reduced proportions amylopectin short chains lower gelatinization temperature enthalpy gelatinization. Overall, these findings serve reinforce efficacy valuable tool improve sustainability with grain

Language: Английский

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Molecular Mechanisms of Rice Seed Germination

New Crops, Journal Year: 2024, Volume and Issue: unknown, P. 100051 - 100051

Published: Sept. 1, 2024

Language: Английский

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Integrated transcriptome and metabolome analysis of salinity tolerance in response to foliar application of choline chloride in rice (Oryza sativa L.)

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 1, 2024

Introduction Salt stress is a major abiotic that affects crop growth and productivity. Choline Chloride (CC) has been shown to enhance salt tolerance in various crops, but the underlying molecular mechanisms rice remain unclear. Methods To investigate regulatory mechanism of CC-mediated rice, we conducted morpho-physiological, metabolomic, transcriptomic analyses on two varieties (WSY, salt-tolerant, HHZ, salt-sensitive) treated with 500 mg·L -1 CC under 0.3% NaCl stress. Results Our results showed foliar application improved morpho-physiological parameters such as root traits, seedling height, strength index, fullness, leaf area, photosynthetic parameters, pigments, starch, fructose content stress, while decreasing soluble sugar, sucrose, sucrose phosphate synthase levels. Transcriptomic analysis revealed regulation combined treatment induced changes expression genes related starch metabolism, citric acid cycle, carbon sequestration organs, antenna proteins both varieties. Metabolomic further supported these findings, indicating photosynthesis, fixation pathways were crucial tolerance. Discussion The metabolomic data suggest enhances by activating distinct transcriptional cascades phytohormone signaling, along multiple antioxidants unique metabolic pathways. These findings provide basis for understanding metabolite synthesis gene response may inform strategies improving resilience

Language: Английский

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Ion homeostasis and coordinated salt tolerance mechanisms in a barley (Hordeum vulgare L.)doubled haploid line

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: Jan. 14, 2025

Salinization poses a significant challenge in agriculture. Identifying salt-tolerant plant germplasm resources and understanding their mechanisms of salt tolerance are crucial for breeding new varieties. However, one the primary obstacles to achieving this goal crops is physiological complexity salt-tolerance trait. In previous study, we developed barley doubled haploid (DH) line, designated as DH20, through mutagenesis combined with microspore culture, establishing it an idea model elucidating tolerance. ion homeostasis, key osmotic agents, antioxidant enzyme activities gene expression were compared between Hua30 (the original material used control) DH20. The results indicated that under treatment, DH20 exhibited significantly higher shoot fresh dry weight, relative height, K+/Na+ ratio, improved stomatal guard cell function, better retention chloroplast ultrastructure Hua30. Notably, K+ efflux was lower while Na+ H+ than those stress mesophyll cells. Furthermore, ascorbate peroxidase, superoxide dismutase, along levels proline, betaine, malondialdehyde, soluble protein, correlated played vital role response stress. Compared Hua30, HvSOS1, HvSOS2, HvSOS3, HvHKT1;3, HvNHX1, HvNHX2, HvNHX3 genes, which showed strong correlation Na+, K+, efflux, differences at 24 h These findings suggest osmolytes, activities, associated coordinated serving important coping present opportunities enhancing salinity tolerance, not only but other cereals well, including wheat rice, by integrating trait traditional mechanisms. MIFE measurements NaCl-induced fluxes from leaf provide breeders efficient method screen potentially crops. Clinical trial number: Not applicable.

Language: Английский

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Genome-Wide Identification and Expression Analysis of Thionin Family in Rice (Oryza sativa) and Functional Characterization of OsTHION15 in Drought Stress and ABA Stress

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 3447 - 3447

Published: April 7, 2025

The OsTHION family represents a class of cysteine-rich signal peptides widely recognized for their significant roles in plant disease resistance and immunity. While members this are known to be induced under various biotic abiotic stresses, responses environmental stressors beyond remain underexplored. This study investigates the evolution, expression patterns, functional gene rice (Oryza sativa) diverse stress conditions. Using sequence data from Phytozome database, we identified 44 classified them into four groups based on phylogenetic analysis. Cis-acting element analysis revealed that promoter regions genes enriched with regulatory elements associated light response, hormone signaling, growth, responses. exhibit complex organ-specific OsTHION30 OsTHION36 showing ubiquitous expression, while other highly expressed specific tissues or developmental stages. Under drought, salt, low-temperature stress, undergo changes, underscoring critical role adaptation challenges. Notably, OsTHION15 was markedly upregulated drought Osthion15 mutant displayed heightened sensitivity ABA confirming its pivotal resistance. RNA sequencing many differentially (DEGs), primarily pathways related ribosomal function suggesting may regulate through multiple mechanisms. In summary, advances our understanding highlights intricate involvement regulating development, These findings offer valuable insights technical support crop improvement, potential applications enhancing adaptability yield stability crops.

Language: Английский

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OsERF2 Acts as a Direct Downstream Target of OsEIL1 to Negatively Regulate Salt Tolerance in Rice

Rice, Journal Year: 2025, Volume and Issue: 18(1)

Published: April 24, 2025

Salinity is a significant limiting factor that adversely affects plant growth, distribution and crop yield. Ethylene responsive factors play crucial roles in responses to tolerance of various abiotic stresses. Recently, we revealed OsERF2 involved root growth by transcriptionally regulating hormone sugar signaling rice. Here, report direct target gene OsEIL1 negatively regulates salt Compared the wild type, gain-of-function mutant (nsf2857) knockdown via an artificial microRNA (Ami-ERF2) exhibited decreased increased tolerance, respectively. The enhanced observed Ami-OsERF2 lines was associated with lower accumulations malondialdehyde reactive oxygen species (ROS) under stress, while opposite true for nsf2857 plants, which tolerance. At transcriptional level, several stress-related genes encoding ROS NAD(P)H-related oxidoreductases were downregulated plants but upregulated Ami-ERF2 plants. Furthermore, yeast one-hybrid ChIP assays can bind EBS cis element present promoter (-bp), suggesting may directly regulate expression OsERF2. Collectively, our findings indicate downstream regulation rice, highlighting its potential application genetic improvement stresses this crop.

Language: Английский

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Mechanisms by Which Exogenous Substances Enhance Plant Salt Tolerance through the Modulation of Ion Membrane Transport and Reactive Oxygen Species Metabolism

Antioxidants, Journal Year: 2024, Volume and Issue: 13(9), P. 1050 - 1050

Published: Aug. 29, 2024

Soil salinization is one of the major abiotic stresses affecting plant growth and development. Plant salt tolerance controlled by complex metabolic pathways. Exploring effective methods mechanisms to improve crop has been a key aspect research on utilization saline soil. Exogenous substances, such as hormones signal transduction can regulate ion transmembrane transport eliminate reactive oxygen species (ROS) reduce stress damage activating various processes. In this review, we summarize which exogenous substances ROS metabolism tolerance. The molecular physiological relationships among in maintaining balance enhancing clearance are examined, trends directions for application improving proposed.

Language: Английский

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OsHAK4 functions in retrieving sodium from the phloem at the reproductive stage of rice

The Plant Journal, Journal Year: 2024, Volume and Issue: 120(1), P. 76 - 90

Published: Aug. 14, 2024

SUMMARY Soil salinity significantly limits rice productivity, but it is poorly understood how excess sodium (Na + ) delivered to the grains at reproductive stage. Here, we functionally characterized OsHAK4 , a member of clade IV HAK/KUP/KT transporter subfamily in rice. was localized plasma membrane and exhibited influx transport activity for Na not K . Analysis organ‐ growth stage‐dependent expression patterns showed that very low levels were detected vegetative stage, its high uppermost node I, peduncle, rachis found Immunostaining indicated localization phloem region rachis. Knockout did affect accumulation However, hak4 mutants accumulated higher rachis, husk, brown compared wild‐type Element imaging revealed peduncle mutants. These results indicate plays crucial role retrieving from upper nodes, thereby preventing distribution stage

Language: Английский

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OsAAH confers salt tolerance in rice seedlings

The Plant Journal, Journal Year: 2024, Volume and Issue: 120(5), P. 1954 - 1968

Published: Oct. 22, 2024

SUMMARY Soil salinization is becoming a great threat that reduces crop productivity worldwide. In this study, we found rice allantoate amidohydrolase ( OsAAH ) expression was significantly upregulated by salt stress, and its overexpression conferred tolerance at the seedling stage. Compared to wild type (WT), contents of ureides (allantoin allantoate) were increased in Osaah mutants reduced lines both before after treatments. Exogenous allantoin promoted overexpression, but not mutants. Subcellular localization showed also localized peroxisomes addition previously reported endoplasmic reticulum (ER). The differential peroxisome‐related genes identified between WT. Furthermore, H 2 O malondialdehyde (MDA) accumulated lines. activities antioxidant enzymes enhanced under NaCl treatment. transcription factor OsABI5 could directly bind promoter activate expression. Our findings reveal be induced stress through activation then confer enhancing scavenging capacity reactive oxygen species (ROS), which contributes breeding tolerance.

Language: Английский

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Strigolactone Alleviates NaCl Stress by Regulating Antioxidant Capacity and Hormone Levels in Rice (Oryza sativa L.) Seedlings

Agriculture, Journal Year: 2024, Volume and Issue: 14(9), P. 1662 - 1662

Published: Sept. 23, 2024

Salt stress is a key environmental factor altering rice plant growth. Strigolactones (GR24) play vital role in responding to various abiotic stresses and regulating However, the regulatory mechanisms of SLs on seedlings under salt have not yet been clarified. A pot experiment was undertaken evaluate effects GR24 soaking variety ‘Huanghuazhan’ (salt-sensitive) seedling growth, antioxidant metabolism, endogenous hormones NaCl stress. Results showed that significantly inhibited growth; disrupted enzymes activity; increased content soluble proteins (SPs), proline (Pro), malondialdehyde (MDA) hydrogen–peroxide (H2O2). improved photosynthetic pigments antioxidant–enzyme activities, including superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), ascorbate–peroxidase (APX); SP, ascorbic acid (AsA); reduced glutathione (GSH) MDA, H2O2, Pro content, resulting mitigation oxidative injury caused by Moreover, strigolactones (SLs), cytokinin (CTK), auxin (IAA), Gibberellin A3 (GA3), IAA/ABA CTK/ABA ratios decreased abscisic (ABA). Findings indicated alleviated damage increasing capacity maintaining balance hormones, thus improving tolerance seedlings.

Language: Английский

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