Protective effects of the postbiotic Lactobacillus plantarum MD35 on bone loss in an ovariectomized mice model DOI Creative Commons

Ju-Yeong Myeong,

Hye-Yeon Jung,

Hyo-Seok Chae

et al.

Research Square (Research Square), Journal Year: 2022, Volume and Issue: unknown

Published: Oct. 21, 2022

Abstract Postmenopausal osteoporosis is caused by estrogen deficiency; it impairs the homeostatic balance of bone, resulting in bone loss owing to increased osteoclastic resorption without a corresponding increase osteoblastic activity. Postbiotics have several pharmaceutical properties, including anti-obesity, anti-diabetic, anti-inflammatory, and anti-osteoporotic activities. However, beneficial effects postbiotic MD35 Lactobacillus plantarum on not been studied. In this study, we demonstrated that MD35, isolated from young radish water kimchi influenced osteoclast differentiation mouse marrow-derived macrophage (BMM) culture. addition, was effective deficiency-induced ovariectomized (OVX) mice, an animal model postmenopausal osteoporosis. BMM cells, inhibited receptor activator nuclear factor-kappa B NF-κB ligand (RANKL)-induced significantly suppressing activity downregulating expression RANKL-mediated osteoclast-related genes; achieved attenuating phosphorylation extracellular signal-related kinase. model, oral administration remarkably improved OVX-induced trabecular alleviated destruction femoral plate growth. Therefore, could be potential therapeutic candidate for osteoclastogenesis through regulation molecular mechanisms.

Language: Английский

Role of Dietary Supplements and Probiotics in Modulating Microbiota and Bone Health: The Gut-Bone Axis DOI Creative Commons
Alessandro de Sire, Roberto de Sire, Claudio Curci

et al.

Cells, Journal Year: 2022, Volume and Issue: 11(4), P. 743 - 743

Published: Feb. 21, 2022

Osteoporosis is characterized by an alteration of bone microstructure with a decreased mineral density, leading to the incidence fragility fractures. Around 200 million people are affected osteoporosis, representing major health burden worldwide. Several factors involved in pathogenesis osteoporosis. Today, altered intestinal homeostasis being investigated as potential additional risk factor for reduced and, therefore, novel therapeutic target. The microflora influences osteoclasts’ activity regulating serum levels IGF-1, while also acting on absorption calcium. It therefore not surprising that gut dysbiosis impacts health. Microbiota alterations affect OPG/RANKL pathway osteoclasts, and correlated strength quality. In this context, it has been hypothesized dietary supplements, prebiotics, probiotics contribute microecological balance important aim present comprehensive review describe state art role supplements agents regulation through microbiota modulation.

Language: Английский

Citations

77
Modulation of bone remodeling by the gut microbiota: a new therapy for osteoporosis DOI Creative Commons
Zhengtian Lyu, Yongfei Hu, Yuming Guo

et al.

Bone Research, Journal Year: 2023, Volume and Issue: 11(1)

Published: June 9, 2023

The gut microbiota (GM) plays a crucial role in maintaining the overall health and well-being of host. Recent studies have demonstrated that GM may significantly influence bone metabolism degenerative skeletal diseases, such as osteoporosis (OP). Interventions targeting modification, including probiotics or antibiotics, been found to affect remodeling. This review provides comprehensive summary recent research on regulating remodeling seeks elucidate regulatory mechanism from various perspectives, interaction with immune system, interplay estrogen parathyroid hormone (PTH), impact metabolites, effect extracellular vesicles (EVs). Moreover, this explores potential therapeutic approach for OP. insights presented contribute development innovative GM-targeted therapies

Language: Английский

Citations

76
Emerging insights into the endocrine regulation of bone homeostasis by gut microbiome DOI Creative Commons
Kirtal Hansdah, Julian C. Lui

Journal of the Endocrine Society, Journal Year: 2024, Volume and Issue: 8(8)

Published: June 11, 2024

Abstract Gut microbiota plays an important role in the regulation of bone homeostasis and health. Recent studies showed that these effects could be mediated through microbial metabolites released by like short-chain fatty acids, metabolism endogenous molecules such as bile or a complex interplay between microbiota, endocrine system, immune system. Importantly, some reciprocal relationship system gut microbiota. For instance, postmenopausal estrogen deficiency lead to dysbiosis which turn affect various response remodeling. In addition, evidence shift indigenous caused antibiotics treatment may also impact normal skeletal growth maturation. this mini-review, we describe recent findings on microbiome homeostasis, with particular focus molecular mechanisms their interactions We will discuss dysbiosis, clinical implications for development new therapeutic strategies osteoporosis other disorders.

Language: Английский

Citations

8
Dietary regulations for microbiota dysbiosis among post-menopausal women with type 2 diabetes DOI Creative Commons
Vineet Singh,

Yeong-Jun Park,

GyuDae Lee

et al.

Critical Reviews in Food Science and Nutrition, Journal Year: 2022, Volume and Issue: 63(29), P. 9961 - 9976

Published: May 30, 2022

Type 2 diabetes (T2D) and T2D-associated comorbidities, such as obesity, are serious universally prevalent health issues among post-menopausal women. Menopause is an unavoidable condition characterized by the depletion of estrogen, a gonadotropic hormone responsible for secondary sexual characteristics in In addition to dimorphism, estrogen also participates glucose–lipid homeostasis, associated with insulin resistance female body. Estrogen level gut regulates microbiota composition, even conjugated actively metabolized estrobolome maintain levels. Moreover, different from pre-menopausal microbiota, it less diverse lacks mucolytic Akkermansia short-chain fatty acid (SCFA) producers Faecalibacterium Roseburia. Through various metabolites (SCFAs, bile acid, serotonin), plays significant role regulating glucose oxidative stress, pro-inflammatory cytokines (IL-1, IL-6). While dysbiosis common women, dietary interventions probiotics, prebiotics, synbiotics can ease dysbiosis. The objective this review understand relationship between factors. Additionally, study provided recommendations avoid T2D progression

Language: Английский

Citations

22
Can probiotics decrease the risk of postmenopausal osteoporosis in women? DOI
Iskandar Azmy Harahap, Joanna Suliburska

PharmaNutrition, Journal Year: 2023, Volume and Issue: 24, P. 100336 - 100336

Published: March 9, 2023

Language: Английский

Citations

12
Protective Effects of the Postbiotic Lactobacillus plantarum MD35 on Bone Loss in an Ovariectomized Mice Model DOI Creative Commons

Ju-Yeong Myeong,

Hye-Yeon Jung,

Hyo-Seok Chae

et al.

Probiotics and Antimicrobial Proteins, Journal Year: 2023, Volume and Issue: 16(2), P. 541 - 551

Published: April 1, 2023

Postmenopausal osteoporosis is caused by estrogen deficiency, which impairs bone homeostasis, resulting in increased osteoclastic resorption without a corresponding increase osteoblastic activity. Postbiotics have several therapeutic properties, including anti-obesity, anti-diabetic, anti-inflammatory, and anti-osteoporotic effects. However, the beneficial effects of postbiotic MD35 Lactobacillus plantarum on not been studied. In this study, we demonstrated that L. MD35, isolated from young radish water kimchi, influences osteoclast differentiation mouse marrow-derived macrophage (BMM) culture. addition, it was effective protecting against deficiency-induced loss ovariectomized (OVX) mice, an animal model postmenopausal osteoporosis. BMM cells, inhibited receptor activator nuclear factor-kappa B NF-κB ligand (RANKL)-induced attenuating phosphorylation extracellular signal-related kinase, significantly suppressing activity down-regulating expression RANKL-mediated osteoclast-related genes. model, oral administration remarkably improved OVX-induced trabecular alleviated destruction femoral plate growth. Therefore, could be potential candidate for osteoclastogenesis through regulation molecular mechanisms.

Language: Английский

Citations

11
Regulation of protein arginine methyltransferase in osteoporosis: a narrative review DOI Creative Commons
Ruiming Wen, Ruiqi Huang, Mingbo Yang

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 13

Published: April 24, 2025

Osteoporosis (OP), a systemic bone disease characterised by increased fragility and susceptibility to fracture, is mainly caused decline in mineral density (BMD) quality an imbalance between formation resorption. Protein arginine methyltransferases (PRMTs) are epigenetic factors post-translational modification (PTM) enzymes participating various biological processes, including mRNA splicing, DNA damage repair, transcriptional regulation, cell signalling. They act catalysing the transfer of residues and, thus, have become therapeutic targets for OP. In-depth studies found that these also play key roles matrix protein metabolism, skeletal proliferation differentiation, signal pathway regulation regulate formation, resorption balance, or both jointly maintain health stability. However, expression changes mechanisms action multiple members PRMT family differ Therefore, this paper discusses functions, action, influencing PRMTs OP, which expected provide new understanding pathogenesis Furthermore, we present theoretical support development more precise effective treatment strategies as well further study molecular PRMTs.

Language: Английский

Citations

0
From Cells to Environment: Exploring the Interplay between Factors Shaping Bone Health and Disease DOI Creative Commons
Samradhi Singh, Devojit Kumar Sarma, Vinod Verma

et al.

Medicina, Journal Year: 2023, Volume and Issue: 59(9), P. 1546 - 1546

Published: Aug. 25, 2023

The skeletal system is an extraordinary structure that serves multiple purposes within the body, including providing support, facilitating movement, and safeguarding vital organs. Moreover, it acts as a reservoir for essential minerals crucial overall bodily function. intricate interplay of bone cells plays critical role in maintaining homeostasis, ensuring delicate balance. However, various factors, both intrinsic extrinsic, can disrupt this physiological process. These factors encompass genetics, aging, dietary lifestyle choices, gut microbiome, environmental toxins, more. They interfere with health through several mechanisms, such hormonal imbalances, disruptions turnover, direct toxicity to osteoblasts, increased osteoclast activity, immune impaired inflammatory responses, disturbances gut–bone axis. As consequence, these give rise range disorders. regulation bone’s functions involves network continuous processes known remodeling, which influenced by extrinsic organism. our understanding precise cellular molecular mechanisms governing complex interactions between host elements affect still its nascent stages. In light this, comprehensive review aims explore emerging evidence surrounding potential risk influencing it, prospective therapeutic interventions future management bone-related

Language: Английский

Citations

9
Phenotype-genotype analysis of Latilactobacills curvatus from different niches: Carbohydrate metabolism, antibiotic resistance, bacteriocin, phage fragments and linkages with CRISPR-Cas systems DOI
Leilei Yu,

Xiaojie Zang,

Ying Chen

et al.

Food Research International, Journal Year: 2022, Volume and Issue: 160, P. 111640 - 111640

Published: July 9, 2022

Language: Английский

Citations

11
Heat-killed Limosilactobacillus reuteri ATCC PTA 6475 prevents bone loss in ovariectomized mice: A preliminary study DOI Creative Commons
Jaqueline Lemes Ribeiro, Thaís Aguiar Santos, Maíra Terra Garcia

et al.

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(5), P. e0304358 - e0304358

Published: May 31, 2024

Osteoporosis is an important health problem that occurs due to imbalance between bone formation and resorption. Hormonal deficiency post-menopause a significant risk factor. The probiotic Limosilactobacillus reuteri has been reported prevent ovariectomy (Ovx)-induced loss in mice reduce postmenopausal women. Despite the numerous benefits of probiotics, as they are live bacteria, administration not risk-free for certain groups (e.g., neonates immunosuppressed patients). We evaluated effects L . (ATCC PTA 6475) its heat-killed (postbiotic) form on Ovx-induced loss. Adult female (BALB/c) were randomly divided into four groups: group C—control (sham); OVX-C—Ovx; OVX-POS—Ovx + probiotic; OVX-PRO—Ovx probiotic. or postbiotic was administered (1.3x10 9 CFU/day) by gavage. Bacterial morphology after heat treatment accessed scanning electron microscopy (SEM). started one week Ovx lasted 28 days (4 weeks). animals euthanized at end period. Bone microarchitecture ileum Occludin pro-inflammatory cytokines gene expression computed microtomography qPCR techniques, respectively. had lower percentage volume (BV/TV) number trabeculae well greater total porosity compared control group. Treatment with resulted higher BV/TV trabecular thickness than caused some cell surface disruptions, but structure resembled SEM analysis. There no statistical differences , Il-6 Tnf-α expression. Both viable prevented ovariectomized mice, independently gut intestinal

Language: Английский

Citations

1