The microbiota ofDrosophila suzukiiinfluences the larval development ofDrosophila melanogaster

PeerJ, Journal Year: 2019, Volume and Issue: 7, P. e8097 - e8097

Published: Nov. 19, 2019

Microorganisms play a central role in the biology of vinegar flies such as Drosophila suzukii and melanogaster: serving food source to both adults larvae, influencing range traits including nutrition, behavior, development. The niches utilized by fly species partially overlap, do microbiota that sustain them, interactions among these players may drive development crop diseases. To learn more about how one affect other, we isolated identified microbes from field-caught D. suzukii, then characterized their effects on melanogaster larval time laboratory. We found consistently included yeasts bacteria. It was dominated genus Hanseniaspora, bacteria families Acetobacteraceae Enterobacteriaceae. Raising under gnotobiotic conditions with each microbial isolate individually, some promoted relative axenic conditions, but most did not have significant effect. In contrast, nearly all tested significantly accelerated exception Starmerella bacillaris, which had opposite effect: slowing developmental rate. investigated basis for this effect examining whether S. bacillaris cells could growth, measuring survival other gut. Our results suggest is digested therefore cannot serve nutrition. These findings interesting implications possible between two Drosophilia nature. Overall, promote development, consistent model infestation fruit can open up habitat melanogaster. propose microbiome an important dimension ecological species.

Language: Английский

---

The impact of the gut microbiome on memory and sleep in Drosophila

Journal of Experimental Biology, Journal Year: 2020, Volume and Issue: unknown

Published: Jan. 1, 2020

The gut microbiome has been proposed to influence diverse behavioral traits of animals, although the experimental evidence is limited and often contradictory. Here, we made use tractability Drosophila melanogaster for both analyses studies test how elimination microorganisms affects a number traits. Relative conventional flies (i.e. with unaltered microbiome), microbiologically sterile (axenic) displayed moderate reduction in memory performance olfactory appetitive conditioning courtship assays. microbiological status had small or no effect on anxiety-like behavior (centrophobism) circadian rhythmicity locomotor activity, but axenic tended sleep longer reduced rebound after deprivation. These last two effects were robust most tests conducted wild-type Canton S w1118 strains, as well using an isogenized panel mutations period gene, which causes altered rhythmicity. Interestingly, absence microbiota few features, notably instantaneous activity speed, varied among strains. Taken together, our findings demonstrate that can have subtle significant specific aspects behavior, some are dependent genetic background.

Language: Английский

---

Why Evolve Reliance on the Microbiome for Timing of Ontogeny?

mBio, Journal Year: 2019, Volume and Issue: 10(5)

Published: Oct. 7, 2019

The timing of life history events has important fitness consequences. Since the 1950s, researchers have combined first principles and data to predict optimal transitions. Recently, a striking mystery emerged. Such transitions can be shaped by completely different branch tree life: species in microbiome. Probing these interactions using testable predictions from evolutionary theory could illuminate whether how host-microbiome integrated histories evolve maintained. Beyond advancing fundamental science, this research program yield applications. In an age microbiome engineering, understanding contexts that lead microbiota signaling shaping ontogeny offer novel mechanisms for manipulations increase agriculture manipulating plant responses stressful environments, or reduce pathogen transmission affecting vector efficiency. We combine evidence essential questions underlying existence microbiome-dependent ontogenetic (MiDOT) fuel on emerging topic.

Language: Английский

---

Common structuring principles of the Drosophila melanogaster microbiome on a continental scale and between host and substrate

Environmental Microbiology Reports, Journal Year: 2020, Volume and Issue: 12(2), P. 220 - 228

Published: Jan. 31, 2020

Summary The relative importance of host control, environmental effects and stochasticity in the assemblage host‐associated microbiomes is being debated. We analysed microbiome among fly populations that were sampled across Europe by European Drosophila Population Genomics Consortium (DrosEU). In order to better understand structuring principles natural D . melanogaster microbiome, we combined data on climate food‐substrate with dense genomic profiling. Food‐substrate, temperature, population structure correlated structure. Microbes, whose abundance was co‐structured populations, also differed between flies their substrate an independent survey. This finding suggests common, host‐related different spatial scales.

Language: Английский

---

Meta-analysis suggests the microbiome responds to Evolve and Resequence experiments in Drosophila melanogaster

BMC Microbiology, Journal Year: 2021, Volume and Issue: 21(1)

Published: April 9, 2021

Experimental evolution has a long history of uncovering fundamental insights into evolutionary processes, but largely neglected one underappreciated component--the microbiome. As eukaryotic hosts evolve, the microbiome may also respond to selection. However, microbial contribution host remains poorly understood. Here, we re-analyzed genomic data characterize metagenomes from ten Evolve and Resequence (E&R) experiments in Drosophila melanogaster determine how changed response selection.Bacterial diversity was significantly different 5/10 studies, primarily traits associated with metabolism or immunity. Duration selection did not influence bacterial diversity, highlighting importance associations specific traits.Our re-analysis suggests often responds selection; thus, contribute E&R experiments. We outline important considerations for incorporating The approach provide critical host-microbiome interactions insight basis adaptation.

Language: Английский

---