TALEs as double-edged swords in plant–pathogen interactions: Progress, challenges, and perspectives

Plant Communications, Journal Year: 2022, Volume and Issue: 3(3), P. 100318 - 100318

Published: March 25, 2022

Xanthomonas species colonize many host plants and cause huge losses worldwide. Transcription activator-like effectors (TALEs) are secreted by translocated into cells to manipulate the expression of target genes, especially oryzae pv. oryzicola, which bacterial blight leaf streak, respectively, in rice. In this review, we summarize progress studies on interaction between hosts, covering both rice other plants. TALEs not only key factors that make susceptible but also essential components plant resistance. Characterization TALE-like proteins has improved our understanding TALE evolution promoted development gene editing tools. addition, interactions hosts have provided strategies possibilities for genetic engineering crop improvement.

Language: Английский

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Soybean hypocotyl elongation is regulated by a MYB33‐SWEET11/21‐GA2ox8c module involving long‐distance sucrose transport

Plant Biotechnology Journal, Journal Year: 2024, Volume and Issue: unknown

Published: June 11, 2024

The length of hypocotyl affects the height soybean and lodging resistance, thus determining final grain yield. However, research on is scarce, regulatory mechanisms are not fully understood. Here, we identified a module controlling transport sucrose, where sucrose acts as messenger moved from cotyledon to hypocotyl, regulating elongation. This comprises four key genes, namely MYB33, SWEET11, SWEET21 GA2ox8c in soybean. In cotyledon, MYB33 responsive promotes expression SWEET11 SWEET21, thereby facilitating hypocotyl. Subsequently, transported up-regulates which ultimately During domestication improvement soybean, an allele with enhanced abilities promote has gradually become enriched landraces cultivated varieties, exhibit high conservation have undergone strong purified selection under artificial selection. Our findings identify new molecular pathway elongation provide insights into mechanism sugar

Language: Английский

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Brown planthopper infestation on rice reduces plant susceptibility to Meloidogyne graminicola by reducing root sugar allocation

New Phytologist, Journal Year: 2024, Volume and Issue: 242(1), P. 262 - 277

Published: Feb. 8, 2024

Summary Plants are simultaneously attacked by different pests that rely on sugars uptake from plants. An understanding of the role plant sugar allocation in these multipartite interactions is limited. Here, we characterized expression patterns sucrose transporter genes and evaluated impact targeted gene mutants brown planthopper (BPH) phloem‐feeding oviposition root BPH‐reduced rice susceptibility to Meloidogyne graminicola . We found OsSUT1 OsSUT2 induced at BPH sites. showed a higher resistance gravid than nymph BPH, this was correlated with callose deposition, as reflected effect M. infection. caused inhibition deposition counteracted oviposition. Meanwhile, pivotal validated cultivar RHT harbouring Bph3 Bph17 In conclusion, demonstrated regulated through differences allocation.

Language: Английский

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OsWRKY26 negatively regulates bacterial blight resistance by suppressing OsXa39 expression

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 9, 2025

Plants are susceptible to infection by various pathogens with high epidemic potential. Xanthomonas oryzae pv. (Xoo) causes bacterial blight in rice, one of the most significant diseases both temperate and tropical regions. In this study, we report identification characterization OsWRKY26, a sucrose-inducible transcription factor, that plays role plant defense responses following Xoo infection. We found mutant plants defective OsWRKY26 showed enhanced response specifically Xoo, indicating factor acts as negative regulator. contrast, did not exhibit higher resistance compared wild-type (WT) when infected rice blast fungal pathogen Magnaporthe oryzae. Transcriptomic analysis WT revealed several genes were upregulated mutants. Of these, selected OsXa39 for further analysis. Transient expression experiments protoplasts repressed Luciferase reporter gene driven promoter. Chromatin immunoprecipitation binds directly promoter region OsXa39. These findings suggest negatively regulates during repressing well other pathogen-related such OsXa47, OsBBR1, OsRSR1, OsPR1a, OsPR1-11, OsPR2, OsPR4c.

Language: Английский

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WRKY36–PIL15 Transcription Factor Complex Negatively Regulates Sheath Blight Resistance and Seed Development in Rice

Plants, Journal Year: 2025, Volume and Issue: 14(4), P. 518 - 518

Published: Feb. 8, 2025

Sheath blight (ShB) causes severe yield loss in rice. Previously, we demonstrated that the sugar will eventually be exported and transporter 11 (SWEET11) mutation significantly improved rice resistance to ShB, but it caused defects seed development. The present study found WRKY36 PIL15 directly activate SWEET11 negatively regulate ShB. Interestingly, interacted with PIL15, activates miR530 a key BR signaling transcription factor WRKY53. AOS2 is an effector protein from Rhizoctonia solani (R. solani) interacts also for nutrition R. solani. These data collectively suggest WRKY36–PIL15 regulates ShB development via activation of miR530, respectively. In addition, are partners by which hijacks

Language: Английский

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The Battle Within: Discovering new insights into Phytopathogen Interactions and Effector Dynamics

Microbiological Research, Journal Year: 2025, Volume and Issue: 298, P. 128220 - 128220

Published: May 15, 2025

Language: Английский

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Ripening and rot: How ripening processes influence disease susceptibility in fleshy fruits

Journal of Integrative Plant Biology, Journal Year: 2024, Volume and Issue: 66(9), P. 1831 - 1863

Published: July 17, 2024

Fleshy fruits become more susceptible to pathogen infection when they ripen; for example, changes in cell wall properties related softening make it easier pathogens infect fruits. The need high-quality fruit has driven extensive research on improving resistance important crops such as tomato (Solanum lycopersicum). In this review, we summarize current progress understanding how during ripening affect by pathogens. These physical barriers that limit entry, the epidermis and its cuticle, along with other defenses growth, preformed induced defense compounds. plant immune system also protects recognizing initiating responses involving reactive oxygen species production, mitogen-activated protein kinase signaling cascades, jasmonic acid, salicylic ethylene, abscisic acid signaling. phytohormones regulate an intricate web of transcription factors (TFs) activate mechanisms, including expression pathogenesis-related genes. tomato, regulators, RIPENING INHIBITOR NON_RIPENING, not only but influence against Moreover, members ETHYLENE RESPONSE FACTOR (ERF) family play pivotal distinct roles defense, different being regulated phytohormones. We discuss interaction ripening-related defense-related TFs Mediator complex. As processes climacteric non-climacteric share many similarities, these have broad applications across fruiting crops. Further individual contributions ERFs will inform efforts diminish disease susceptibility ripe fruit, satisfy growing demand decrease food waste economic losses.

Language: Английский

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CRISPR/Cas9 and its derivatives to improve crop biotic stress resistance: Current status and prospects

Physiological and Molecular Plant Pathology, Journal Year: 2024, Volume and Issue: unknown, P. 102482 - 102482

Published: Nov. 1, 2024

Language: Английский

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Cell death induced by mycotoxin fumonisin B1 is accompanied by oxidative stress and transcriptional modulation in Arabidopsis cell culture

Plant Cell Reports, Journal Year: 2022, Volume and Issue: 41(8), P. 1733 - 1750

Published: June 25, 2022

Fumonisin B1 induces rapid programmed cell death in Arabidopsis cells, oxidative and nitrosative bursts, differentially modulates responsive genes. Glutathione is the main antioxidant involved stress response. (FB1) a fungal toxin produced by Fusarium spp. able to exert pleiotropic toxicity plants. FB1 known be strong inducer of (PCD); however, exact mechanism underling plant-toxin interactions molecular events that lead PCD are still unclear. Therefore, this work, we provided comprehensive investigation response model organism thaliana at nuclear, transcriptional, biochemical level after treatment with two different concentrations, namely 1 5 µM during time-course 96 h. induced bursts cultures, which resembled HR-like event. Different genes regulation PCD, metabolism, photosynthesis, pathogenesis, sugar transport were upregulated, especially late time higher concentration. Among enzymes compounds studied, only glutathione appeared highly both treatments, suggesting it might an important molecule exposure. Collectively, these findings highlight complexity signaling network A. provide information for understanding physiological, molecular, responses counteract FB1-induced toxicity.

Language: Английский

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Advances in the Characterization of the Mechanism Underlying Bacterial Canker Development and Tomato Plant Resistance

Horticulturae, Journal Year: 2022, Volume and Issue: 8(3), P. 209 - 209

Published: Feb. 27, 2022

Bacterial canker caused by the Gram-positive actinobacterium Clavibacter michiganensis is one of most serious bacterial diseases tomatoes, responsible for 10–100% yield losses worldwide. The pathogen can systemically colonize tomato vascular bundles, leading to wilting, cankers, bird’s eye lesions, and plant death. Bactericidal agents are insufficient managing this disease, because rapidly migrate through system plants induce systemic symptoms. Therefore, use resistant cultivars necessary controlling disease. We herein summarize pathogenicity C. in molecular basis pathogenesis. Moreover, advances characterization resistance tomatoes introduced, status genetics-based research described. Finally, we propose potential future on resistance. More specifically, there a need thorough analysis host–pathogen interaction, accelerated identification annotation genes mechanisms, diversification resources or exhibiting broad-spectrum disease resistance, production novel effective control prevention. This review provides researchers with relevant information breeding cankers.

Language: Английский

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