Genomic analyses elucidate the causes and consequences of breakdown of distyly inLinum trigynum DOI Creative Commons
Juanita Gutiérrez‐Valencia, Panagiotis‐Ioannis Zervakis, Zoé Postel

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 17, 2023

Abstract Distyly is an iconic floral polymorphism governed by a supergene, which promotes efficient pollen transfer and outcrossing through reciprocal differences in the position of sexual organs flowers, often coupled with heteromorphic self-incompatibility (SI). has evolved convergently multiple flowering plant lineages, but also broken down repeatedly, resulting homostylous, self-compatible populations elevated rates self-fertilization. Here, we aimed to study genetic causes genomic consequences shift homostyly Linum trigynum , closely related distylous tenue. Building on high-quality genome assembly, show that L. harbors region homologous dominant haplotype distyly supergene conferring long stamens short styles tenue suggesting loss first occurred short-styled individual. In contrast homostylous Primula Fagopyrum no fixed loss-of-function mutations coding sequences S- linked candidate genes. Instead, gene expression analyses controlled crosses suggest downregulating LtWDR-44 for male SI and/or anther height could underlie self-compatibility (SC) . Population 224 whole-genome further demonstrate highly self-fertilizing, exhibits significantly lower diversity genome-wide, experiencing relaxed purifying selection less frequent positive nonsynonymous relative Our shed light advance our understanding common evolutionary transition plants.

Language: Английский

Convergent evolutionary patterns of heterostyly across angiosperms support the pollination-precision hypothesis DOI Creative Commons
Violeta I. Simón‐Porcar, Marcial Escudero, Rocío Santos‐Gally

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Feb. 9, 2024

Since the insights by Charles Darwin, heterostyly, a floral polymorphism with morphs bearing stigmas and anthers at reciprocal heights, has become model system for study of natural selection. Based on his archetypal heterostylous flower, including regular symmetry, few stamens tube, Darwin hypothesised that heterostyly evolved to promote outcrossing through efficient pollen transfer between involving different areas pollinator's body, thus proposing seminal pollination-precision hypothesis. Here we update number other style-length polymorphic taxa 247 genera belonging 34 families, notably expanding known cases 20%. Using phylogenetic comparative analyses across angiosperms, show numerous independent origins associated actinomorphic, tubular flowers low sex organs, fused corolla, pollination long-tongued insects. These associations provide support Darwinian hypothesis as basis convergent evolution angiosperms.

Language: Английский

Citations

12
Genomic analyses elucidate S‐locus evolution in response to intra‐specific losses of distyly in Primula vulgaris DOI Creative Commons
Emiliano Mora‐Carrera, Rebecca L. Stubbs, Giacomo Potente

et al.

Ecology and Evolution, Journal Year: 2024, Volume and Issue: 14(3)

Published: March 1, 2024

Distyly, a floral dimorphism that promotes outcrossing, is controlled by hemizygous genomic region known as the

Language: Английский

Citations

6
Haplotype‐resolved genome assembly provides insights into the evolution of S‐locus supergene in distylous Nymphoides indica DOI Open Access
Jingshan Yang, Haoran Xue, Zhizhong Li

et al.

New Phytologist, Journal Year: 2023, Volume and Issue: 240(5), P. 2058 - 2071

Published: Sept. 17, 2023

Distyly has evolved independently in numerous animal-pollinated angiosperm lineages. Understanding of its molecular basis been restricted to a few species, primarily Primula. Here, we investigate the genetic architecture single diallelic locus (S-locus) supergene, linkage group functionally associated genes, and explore how it may have distylous Nymphoides indica, lineage flowering plants not previously investigated. We assembled haplotype-resolved genomes, used read-coverage-based genome-wide association study (rb-GWAS) locate S-locus co-expression network analysis gene networks underpinning development distyly, comparative genomic analyses origins supergene. identified three linked candidate genes - NinBAS1, NinKHZ2, NinS1 that were only evident short-styled morph hemizygous. Co-expression suggested brassinosteroids contribute dimorphic sex organs morph. Comparative indicated supergene likely via stepwise duplications affected by transposable element activities. Our provides novel insight into structure, regulation, evolution governing distyly N. indica. It also high-quality resources for future research on mechanisms underlying striking evolutionary convergence form function across heterostylous taxa.

Language: Английский

Citations

13
A hemizygous supergene controls homomorphic and heteromorphic self-incompatibility systems in Oleaceae DOI
Pauline Raimondeau,

Sayam Ksouda,

William Marande

et al.

Current Biology, Journal Year: 2024, Volume and Issue: 34(9), P. 1977 - 1986.e8

Published: April 15, 2024

Language: Английский

Citations

4
The homomorphic self-incompatibility system in Oleaceae is controlled by a hemizygous genomic region expressing a gibberellin pathway gene DOI
Vincent Castric, Rita A. Batista, Amélie Carré

et al.

Current Biology, Journal Year: 2024, Volume and Issue: 34(9), P. 1967 - 1976.e6

Published: April 15, 2024

Language: Английский

Citations

4
Rapid detection of RNase‐based self‐incompatibility in Lysimachia monelli (Primulaceae) DOI Creative Commons
Karolis Ramanauskas, Francisco Javier Jiménez‐López, Mercedes Sánchez‐Cabrera

et al.

American Journal of Botany, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 13, 2025

Abstract Premise Primroses famously employ a system that simultaneously expresses distyly and filters out self‐pollen. Other species in the Primulaceae family, including Lysimachia monelli (blue pimpernel), also express self‐incompatibility (SI), but involving with distinct features an unknown molecular genetic basis. Methods We utilize candidate‐based transcriptome sequencing (RNA‐seq) approach, relying on candidate T2/S‐RNase Class III S‐linked F‐box‐motif‐containing genes harnessing unusual evolutionary of SI, to examine whether RNase‐based mechanism underlies SI L. . term this approach "SI detection RNA‐seq" (SIDR). Results The results sequencing, crossing, population genetics, each support causal association linking recovered genotypes phenotypes. finding (Ericales) all cements long‐held view was present ancestral pentapetal eudicot, whose descendants now comprise two‐thirds angiosperms. It significantly narrows plausible maximum age for heterostyly evolution within family. Conclusions SIDR is powerful, flexible, inexpensive, most critically enables work often‐neglected species. may be used or without close enormous gaps understanding basis history breeding evolution.

Language: Английский

Citations

0
The Monochoria genome provides insights into the molecular mechanisms underlying floral heteranthery DOI
Jingshan Yang, Jinming Chen, Xiangyan He

et al.

Journal of genetics and genomics/Journal of Genetics and Genomics, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

Citations

0
Does the occurence of homostyly necessarily accompany the breakdown of heteromorphic incompatibility system? DOI Creative Commons
Jing Zhao,

Laiziti Kuliku,

Zhang Ai-qin

et al.

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 27, 2025

Heterostyly is a genetic polymorphism that facilitates precise pollen transfer through reciprocal herkogamy. The loss or variation of herkogamy usually accompanied by the breakdown heteromorphic incompatibility system. Homostyly, which characterized self-compatibility and same stigma-anther height common floral morph in evolution heterostyly. Limonium aureum distylous species distributed desert northwest China, with (H-morph) widely natural populations, resembling classical homostyly. aim this study was to clarify whether occurrence H-morph also system, relationship between long-styled-/shortstyled-morph (L-/S-morph). morphs composition frequency, heterostylous syndrome, pollinators visiting efficiency were investigated five populations L. based on field observation, artificial control pollination experiment so on. All composed L-, S- H-morphs, except for ATS population only H-morph, there significant differences flower size parameter, fruit set, degree limitation, while no among within population. However, each demonstrated dimorphic pollen-stigma morphology strict especially population, they compatible morphology, regardless herkogamy, vice versa. It speculated different may be at stages evolution. without stage before distyly formation, other 4 after formation. caused separation shortening L- S-morph populations. These phenomenons independent physiological breakdown, as well coexistence from origins evolutionary have been reported first time Plumbaginaceae.

Language: Английский

Citations

0
Genomic studies inLinumshed light on the evolution of the distyly supergene and the molecular basis of convergent floral evolution DOI Creative Commons
Panagiotis‐Ioannis Zervakis, Zoé Postel, Aleksandra Losvik

et al.

Published: April 17, 2025

Summary Distyly, an example of convergent evolution, is governed by a supergene called the S- locus. Recent studies highlight similar genetic architectures independently evolved S -loci, but whether regulatory pathways underlie evolution distyly remains unclear. We examined supergenes and mechanisms underlying in Linum species that diverged ∼33 Mya. Using haplotype-resolved genomes population genomics, we identified characterized loci perenne (distylous) grandiflorum (style length dimorphic), compared them to tenue (distylous). then tested for conserved hormonal mechanism regulating style polymorphism . Hemizygosity short-styled individuals shared feature -locus supergene, though its size, gene content, repeat elements, extent recombination suppression vary greatly among species. Two candidate genes, TSS1 length) WDR-44 (anther height/pollen self-incompatibility) are at Consistent with brassinosteroid-dependent role , epibrassinolide treatment revealed conserved, morph-specific effect on length. locus architecture, key genes remain >30 Mya In combination findings from other systems, our results suggest brassinosteroid pathway frequently contributes polymorphism.

Language: Английский

Citations

0
Integrative Taxonomy for Species Delimitation: A Case Study in Two Widely Accepted Yet Morphologically Confounding Rosa Species Within Sect. Pimpinellifoliae (Rosaceae) DOI
Yu-Mei Yuan, Yu Feng,

Jingbo Wang

et al.

Molecular Ecology, Journal Year: 2025, Volume and Issue: unknown

Published: April 26, 2025

ABSTRACT The use of morphological traits as a practical approach for delimiting taxa at various ranks has long been regarded reliable basis taxonomy. However, its efficacy increasingly called into question in many taxonomic groups due to inherent limitations, such failing account phenotypic plasticity, ecologically driven variation (e.g., ecotypes), and parallel evolution. These factors often introduce ambiguity or misleading similarities, thereby obscuring the true evolutionary relationships among taxa, particularly context species delimitation. In present study, we employ an integrated methodology that combines quantitative analyses, whole‐genome data, ecological measurements resolve boundaries two morphologically similar roses, Rosa sericea hugonis , which have considered distinct but lack clear boundaries. Our findings reveal unbiased analysis data based on large representative sample size was insufficient identify effective diagnostic traits. when complemented with genome‐wide population‐level sequencing geographic niche assessments, delineation significantly improved. Furthermore, provide additional insight abiotic driving interspecific intraspecific divergence. By integrating multiple lines evidence—spanning genomic (intrinsic) (extrinsic) traits—and incorporating interaction between their environments, can be delineated greater confidence. A well‐defined thus established through mutual corroboration diverse datasets, ensuring more rigorous comprehensive framework.

Language: Английский

Citations

0