Black rice diet alleviates colorectal cancer development through modulating tryptophan metabolism and activating AHR pathway

iMeta, Journal Year: 2024, Volume and Issue: 3(1)

Published: Jan. 15, 2024

Abstract Consumption of dietary fiber and anthocyanin has been linked to a lower incidence colorectal cancer (CRC). This study scrutinizes the potential antitumorigenic attributes black rice diet (BRD), abundantly rich in anthocyanin. Our results demonstrate notable effects mice on BRD, indicated by reduction both size number intestinal tumors consequent extension life span, compared control diet‐fed counterparts. Furthermore, fecal transplants from BRD‐fed germ‐free led decrease colonic cell proliferation, coupled with maintained integrity barrier. The BRD was associated significant shifts gut microbiota composition, specifically an augmentation probiotic strains Bacteroides uniformis Lactobacillus . Noteworthy changes metabolites were also documented, including upregulation indole‐3‐lactic acid indole. These have identified stimulate aryl hydrocarbon receptor pathway, inhibiting CRC proliferation tumorigenesis. In summary, these findings propose that may modulate progression fostering protective metabolite profiles. accentuates health advantages whole‐grain foods, emphasizing utility promoting health.

Language: Английский

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Advances in the isolation, cultivation, and identification of gut microbes

Military Medical Research, Journal Year: 2024, Volume and Issue: 11(1)

Published: June 3, 2024

Abstract The gut microbiome is closely associated with human health and the development of diseases. Isolating, characterizing, identifying microbes are crucial for research on essential advancing our understanding utilization it. Although culture-independent approaches have been developed, a pure culture required in-depth analysis disease mechanisms biotherapy strategies. Currently, faces challenge expanding existing database culturable microbiota rapidly isolating target microorganisms. This review examines advancements in microbe isolation cultivation techniques, such as culturomics, droplet microfluidics, phenotypic genomics selection, membrane diffusion. Furthermore, we evaluate progress made technology considering both non-targeted targeted focus future microbial culturomics expected to be high-throughput, automation, integration. Advancements this field may facilitate strain-level investigation into underlying diseases related microbiota.

Language: Английский

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Structure properties of Canna edulis RS3 (double enzyme hydrolysis) and RS4 (OS-starch and cross-linked starch): Influence on fermentation products and human gut microbiota

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 265, P. 130700 - 130700

Published: March 7, 2024

Language: Английский

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Distinct Gut Microbial Signature and Host Genetic Variants in Association with Liver Fibrosis Severity in Patients with MASLD

Nutrients, Journal Year: 2024, Volume and Issue: 16(12), P. 1800 - 1800

Published: June 7, 2024

Gut microbiota might affect the severity and progression of metabolic dysfunction-associated steatotic liver disease (MASLD). We aimed to characterize gut dysbiosis clinical parameters regarding fibrosis stages assessed by magnetic resonance elastography. This study included 156 patients with MASLD, stratified into no/mild (F0–F1) moderate/severe (F2–F4). Fecal specimens were sequenced targeting V4 region 16S rRNA gene analyzed using bioinformatics. The genotyping PNPLA3, TM6SF2, HSD17B13 was allelic discrimination assays. Our data showed that microbial profiles between groups significantly differed in beta-diversity but not alpha-diversity indices. Enriched Fusobacterium Escherichia_Shigella, depleted Lachnospira found F2–F4 group versus F0–F1 group. Compared F0–F1, had elevated plasma surrogate markers epithelial permeability bacterial translocation. genera, PNPLA3 polymorphisms, old age, diabetes independently associated advanced multivariable analyses. Using Random Forest classifier, signature three genera could differentiate high diagnostic accuracy (AUC 0.93). These results indicated imbalance enriched pathogenic decreased beneficial bacteria, association several genetic factors, potential contributors pathogenesis MASLD.

Language: Английский

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Obesity and the gut microbiota: implications of neuroendocrine and immune signaling

FEBS Journal, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 19, 2024

Obesity is a major health challenge due to its high prevalence and associated comorbidities. The excessive intake of diet rich in fat sugars leads persistent imbalance between energy expenditure, which increases adiposity. Here, we provide an update on relevant diet-microbe-host interactions contributing or protecting from obesity. In particular, focus how unhealthy diets shape the gut microbiota thus impact crucial intestinal neuroendocrine immune system functions. We describe these promote dysfunction gut-to-brain pathways involved food control postprandial metabolism elevate proinflammatory tone, promoting obesity metabolic complications. addition, examples this knowledge may inspire microbiome-based interventions, such as fecal transplants, probiotics, biotherapeutics, effectively combat obesity-related disorders. also discuss current limitations gaps research

Language: Английский

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Bioactive Compounds from Brown Algae Alleviate Nonalcoholic Fatty Liver Disease: An Extensive Review

Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: 71(4), P. 1771 - 1787

Published: Jan. 23, 2023

Nonalcoholic fatty liver disease (NAFLD) is one of the most prevalent chronic diseases. The increasing NAFLD incidences are associated with unhealthy lifestyles. Currently, there no effective therapeutic options for NAFLD. Thus, a need to develop safe, efficient, and economic treatment Brown algae, which edible, contain abundant bioactive compounds, including polysaccharides phlorotannins. They have been shown ameliorate insulin resistance, as well hepatic steatosis, all these biological functions can potentially alleviate Accumulating reports that dietary consumption brown algae reduces risk development. In this review, we summarized animal experiments clinical proof their compounds within past decade. Our findings show possible avenues further research into pathophysiology therapy.

Language: Английский

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Gut microbiota causally affects cholelithiasis: a two-sample Mendelian randomization study

Frontiers in Cellular and Infection Microbiology, Journal Year: 2023, Volume and Issue: 13

Published: Oct. 9, 2023

The gut microbiota is closely linked to cholesterol metabolism-related diseases such as obesity and cardiovascular diseases. However, whether plays a causal role in cholelithiasis remains unclear.This study explored the relationship between cholelithiasis. We hypothesize that influences development.A two-sample Mendelian randomization method was combined with STRING analysis test this hypothesis. Summary data on were obtained from MiBioGen (n=13,266) FinnGen R8 consortia (n=334,367), respectively.Clostridium senegalense, Coprococcus3, Lentisphaerae increased risk of expressed more bile salt hydrolases. In contrast, Holdemania, Lachnospiraceae UCG010, Ruminococcaceae NK4A214 weakly hydrolases implied have protective effect against by analysis.Gut causally may be related This work improves our understanding causality facilitate development treatment strategies.

Language: Английский

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Causality Investigation between Gut Microbiota, Derived Metabolites, and Obstructive Sleep Apnea: A Bidirectional Mendelian Randomization Study

Nutrients, Journal Year: 2023, Volume and Issue: 15(21), P. 4544 - 4544

Published: Oct. 26, 2023

Various studies have highlighted the important associations between obstructive sleep apnea (OSA) and gut microbiota related metabolites. Nevertheless, establishment of causal relationships these remains to be determined. Multiple mendelian randomization (MR) analyses were performed genetically predict causative impact 196 83 metabolites on OSA. Two-sample MR was used assess potential association, causality evaluated using inverse variance weighted (IVW), MR-Egger, median (WM) methods. Multivariable (MVMR) employed ascertain independence linked Additionally, Cochran’s Q test, Egger intercept test Steiger for sensitivity analyses. The analysis revealed that genus_Ruminococcaceae (UCG009) (PIVW = 0.010) genus_Subdoligranulum 0.041) associated with an increased risk OSA onset. Conversely, Family_Ruminococcaceae 0.030), genus_Coprococcus2 (PWM 0.025), genus_Eggerthella 0.011), genus_Eubacterium (xylanophilum_group) 0.001) negatively Among evaluated, 3-dehydrocarnitine, epiandrosterone sulfate, leucine determined independent factors Moreover, reverse demonstrated a suggestive association exposure six taxa. This study offers compelling evidence regarding beneficial or detrimental its risk, thereby providing new insights into mechanisms microbiome-mediated development.

Language: Английский

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The essential roles of FXR in diet and age influenced metabolic changes and liver disease development: a multi-omics study

Biomarker Research, Journal Year: 2023, Volume and Issue: 11(1)

Published: Feb. 18, 2023

Aging and diet are risks for metabolic diseases. Bile acid receptor farnesoid X (FXR) knockout (KO) mice develop liver diseases that progress into cancer as they age, which is accelerated by Western (WD) intake. The current study uncovers the molecular signatures age-linked disease development in an FXR-dependent manner.Wild-type (WT) FXR KO male mice, either on a healthy control (CD) or WD, were euthanized at ages of 5, 10, 15 months. Hepatic transcriptomics, liver, serum, urine metabolomics well microbiota profiled.WD intake facilitated hepatic aging WT mice. In manner, increased inflammation reduced oxidative phosphorylation primary pathways affected WD aging. has role modulating B cell-mediated humoral immunity was enhanced Moreover, dictated neuron differentiation, muscle contraction, cytoskeleton organization addition to metabolism. There 654 transcripts commonly altered diets, ages, KO, 76 them differentially expressed human hepatocellular carcinoma (HCC) livers. Urine metabolites differentiated dietary effects both genotypes, serum clearly separated irrespective diets. amino metabolism TCA cycle. essential colonization age-related gut microbes. Integrated analyses uncovered bacteria linked with intake, aging, related HCC patient survival.FXR target prevent age-associated disease. microbes can be diagnostic markers

Language: Английский

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Gut Bacterial Communities in HIV-Infected Individuals with Metabolic Syndrome: Effects of the Therapy with Integrase Strand Transfer Inhibitor-Based and Protease Inhibitor-Based Regimens

Microorganisms, Journal Year: 2023, Volume and Issue: 11(4), P. 951 - 951

Published: April 6, 2023

Antiretroviral therapies (ART) are strongly associated with weight gain and metabolic syndrome (MetS) development in HIV-infected patients. Few studies have evaluated the association between gut microbiota integrase strand transfer inhibitor (INSTI)-based protease (PI)-based regimens patients MetS. To assess this, fecal samples were obtained from treated different (16 PI + MetS or 30 INSTI MetS) 18 healthy controls (HCs). The microbial composition was characterized using 16S rRNA amplicon sequencing. INSTI-based PI-based a significant decrease α-diversity compared to HCs. group showed lowest both regimens. A increase abundance of short-chain fatty acid (SCFA)-producing genera (Roseburia, Dorea, Ruminococcus torques, Coprococcus) observed group, while Prevotella, Fusobacterium, Succinivibrio significantly increased group. Moreover, Proteobacteria/Firmicutes ratio overrepresented, functional pathways related biosynthesis LPS components receiving INSTIs more pronounced dysbiosis orchestrated by decreased bacterial richness diversity, an almost complete absence SCFA-producing bacteria alterations pathways. These findings not been previously observed.

Language: Английский

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