Shenfu injection improves isoproterenol-induced heart failure in rats by modulating co-metabolism and regulating the trimethylamine-N-oxide - inflammation axis DOI Creative Commons
Li Lin, Jiahao Ye, Zhenyu Zhao

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: June 20, 2024

Heart failure (HF) is a chronic condition that progressively worsens and continues to be major financial burden public health concern. The “gut-heart” axis provides an innovative perspective therapeutic strategy for preventing treating heart failure. Shenfu injection (SFI) Traditional Chinese Medicine-based treatment demonstrating potential as However, the precise mechanisms of SFI in are not completely characterized. In this study, HF models were established utilizing subcutaneous multipoint isoproterenol (ISO) at dosage 5 mg kg −1 ·d 7 days. Serum levels inflammatory biomarkers quantified using protein microarrays. Rat feces analyzed untargeted metabolomics research 16S rRNA sequencing. link between gut microbiota metabolites was examined MetOrigin Spearman correlation analysis. Our results show effectively enhances cardiac function rats with ISO-induced by potentially modulating pro-/anti-inflammatory imbalance reducing serum urine Trimethylamine-N-oxide (TMAO) levels. Moreover, significantly increases abundance Bacteroidota phylum level, thereby improving disrupted composition. Additionally, supplementation enriches specific genera known their capacity produce short-chain fatty acids. found associated three key metabolic pathways, revealed fecal metabonomics analysis, including pentose phosphate pathway, pyrimidine metabolism, purine metabolism. Metabolite tracing analysis Taurine hypotaurine metabolism microbial community. biosynthesis Pyrimidine Purine beta-alanine Naphthalene degradation, Pantothenate, CoA identified co-metabolic pathways microbes host. also correlated differentially expressed regulated microbiota. These suggest improves co-metabolism regulating TMAO-inflammation axis.

Language: Английский

Gut Microbiota Patterns in Patients with Non-Alcoholic Fatty Liver Disease: A Comprehensive Assessment Using Three Analysis Methods DOI Open Access
А. В. Коробейникова, Olga A. Zlobovskaya, А.Ф. Шептулина

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(20), P. 15272 - 15272

Published: Oct. 17, 2023

Non-alcoholic fatty liver disease (NAFLD) is considered the most common chronic worldwide, affecting nearly 25% of global adult population. Increasing evidence suggests that functional and compositional changes in gut microbiota may contribute to development promote progression NAFLD. 16S rRNA gene next-generation sequencing widely used determine specific features NAFLD microbiome, but a complex system such as requires comprehensive approach. We three different approaches: MALDI-TOF-MS bacterial cultures, qPCR, NGS sequencing, well wide variety statistical methods assess differences composition between patients without significant fibrosis control group. The listed showed enrichment Collinsella sp. Oscillospiraceae for samples Lachnospiraceae (and particular Dorea sp.) Veillonellaceae families, Bifidobacteriaceae, Lactobacillaceae, Enterococcaceae (particularly Enterococcus faecium faecalis), were also found be important taxa microbiome evaluation. Considering individual method observations, an increase Candida krusei decrease Bacteroides uniformis detected using MALDI-TOF-MS. An Gracilibacteraceae, Chitinophagaceae, Pirellulaceae, Erysipelatoclostridiaceae, Muribaculaceae, Comamonadaceae, Acidaminococcaceae observed with NGS, Fusobacterium nucleatum was shown qPCR analysis. These findings confirm associated composition. Further investigations are required cause-and-effect relationships impact microbiota-derived compounds on

Language: Английский

Citations

13
Potential biomarkers of acute myocardial infarction based on the composition of the blood microbiome DOI

R Z Chen,

Yonglong Ye,

Yali Ding

et al.

Clinica Chimica Acta, Journal Year: 2024, Volume and Issue: 556, P. 117843 - 117843

Published: Feb. 21, 2024

Language: Английский

Citations

5
Extract of Gardenia jasminoides Ellis Attenuates High-Fat Diet-Induced Glycolipid Metabolism Disorder in Rats by Targeting Gut Microbiota and TLR4/Myd88/NF-κB Pathway DOI Creative Commons
Chenghao Lv, Xin Liu, Shiyun Chen

et al.

Antioxidants, Journal Year: 2024, Volume and Issue: 13(3), P. 293 - 293

Published: Feb. 28, 2024

Gardenia jasminoides Ellis is abundant in crocin and has a longstanding historical usage both as dietary natural ethnic medicine. Enhanced studies have increasingly revealed the intricate interplay between glycolipid metabolism gut microbiota, wherein their imbalance regarded pivotal indicator of metabolic disorders. Currently, precise molecular mechanism crude extract from (GC) targeting microbiota to regulate disorder still unclear. Firstly, we explored effect GC on digestive enzymes (α-amylase α-glucosidase) vitro. Secondly, investigated physical chemical parameters high-fat diet (HFD) rats, such body weight change, fasting blood glucose lipid levels, liver oxidative stress injury. Then, 16S rDNA sequencing was used analyze effects composition structure microbiota. Finally, impact TLR4/Myd88/NF-κB signaling pathway intestine assessed by Western Blotting. In present study, found exhibit hypoglycemic vitro, inhibition enzymes. animal experiments, observed that significantly reduced glucose, TC, TG levels while increasing HDL-C levels. Additionally, demonstrated hepatoprotective properties enhancing antioxidative capacity through upregulation SOD, CAT, GSH-Px, reducing ROS. results showed had significant HFD mainly ratio Firmicutes/Bateroidota, affected genera related metabolism, Akkermansia, Ligilactobacillus, Lactobacillus, Bacteroides, Prevotellaceae, etc. The Blotting effectively downregulated protein expressions TLR4, Myd88, NF-κB indicating could target interfere with disorder. Correlation analysis Akkermansia-TLR4/Myd88/NF-κB axis which attenuates Therefore, this study establishes foundation for novel therapeutic agent chemoprevention, it introduces methodology harnessing potential botanical extracts prevention treatment syndrome.

Language: Английский

Citations

5
Effects of probiotics, prebiotics and synbiotics on anthropometric, cardiometabolic and inflammatory markers: An umbrella review of meta-analyses DOI

Yu Qing Jolene Teo,

Bryan Chong,

Rou Yi Soong

et al.

Clinical Nutrition, Journal Year: 2024, Volume and Issue: 43(6), P. 1563 - 1583

Published: May 10, 2024

Language: Английский

Citations

5
Shenfu injection improves isoproterenol-induced heart failure in rats by modulating co-metabolism and regulating the trimethylamine-N-oxide - inflammation axis DOI Creative Commons
Li Lin, Jiahao Ye, Zhenyu Zhao

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: June 20, 2024

Heart failure (HF) is a chronic condition that progressively worsens and continues to be major financial burden public health concern. The “gut-heart” axis provides an innovative perspective therapeutic strategy for preventing treating heart failure. Shenfu injection (SFI) Traditional Chinese Medicine-based treatment demonstrating potential as However, the precise mechanisms of SFI in are not completely characterized. In this study, HF models were established utilizing subcutaneous multipoint isoproterenol (ISO) at dosage 5 mg kg −1 ·d 7 days. Serum levels inflammatory biomarkers quantified using protein microarrays. Rat feces analyzed untargeted metabolomics research 16S rRNA sequencing. link between gut microbiota metabolites was examined MetOrigin Spearman correlation analysis. Our results show effectively enhances cardiac function rats with ISO-induced by potentially modulating pro-/anti-inflammatory imbalance reducing serum urine Trimethylamine-N-oxide (TMAO) levels. Moreover, significantly increases abundance Bacteroidota phylum level, thereby improving disrupted composition. Additionally, supplementation enriches specific genera known their capacity produce short-chain fatty acids. found associated three key metabolic pathways, revealed fecal metabonomics analysis, including pentose phosphate pathway, pyrimidine metabolism, purine metabolism. Metabolite tracing analysis Taurine hypotaurine metabolism microbial community. biosynthesis Pyrimidine Purine beta-alanine Naphthalene degradation, Pantothenate, CoA identified co-metabolic pathways microbes host. also correlated differentially expressed regulated microbiota. These suggest improves co-metabolism regulating TMAO-inflammation axis.

Language: Английский

Citations

5