Plant pangenomes for crop improvement, biodiversity and evolution

Nature Reviews Genetics, Journal Year: 2024, Volume and Issue: 25(8), P. 563 - 577

Published: Feb. 20, 2024

Language: Английский

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Variation in TaSPL6-D confers salinity tolerance in bread wheat by activating TaHKT1;5-D while preserving yield-related traits

Nature Genetics, Journal Year: 2024, Volume and Issue: 56(6), P. 1257 - 1269

Published: May 27, 2024

Language: Английский

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Genomics-informed prebreeding unlocks the diversity in genebanks for wheat improvement

Nature Genetics, Journal Year: 2022, Volume and Issue: 54(10), P. 1544 - 1552

Published: Oct. 1, 2022

Language: Английский

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A catalogue of resistance gene homologs and a chromosome‐scale reference sequence support resistance gene mapping in winter wheat

Plant Biotechnology Journal, Journal Year: 2022, Volume and Issue: 20(9), P. 1730 - 1742

Published: May 14, 2022

A resistance gene atlas is an integral component of the breeder's arsenal in fight against evolving pathogens. Thanks to high-throughput sequencing, catalogues genes can be assembled even crop species with large and polyploid genomes. Here, we report on capture sequencing assembly homologs a diversity panel 907 winter wheat genotypes comprising ex situ genebank accessions current elite cultivars. In addition, use accurate long-read chromosome conformation construct chromosome-scale genome sequence cv. Attraktion, variety representative European wheat. We illustrate value our resource for breeders geneticists by (i) comparing complements plant genetic resources varieties (ii) conducting genome-wide associations scans (GWAS) fungal diseases yellow rust leaf using reference-based reference-free GWAS approaches. The content under peaks was scrutinized Attraktion.

Language: Английский

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Genome resources for the elite bread wheat cultivar Aikang 58 and mining of elite homeologous haplotypes for accelerating wheat improvement

Molecular Plant, Journal Year: 2023, Volume and Issue: 16(12), P. 1893 - 1910

Published: Oct. 28, 2023

Language: Английский

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Origin and evolution of the bread wheat D genome

Nature, Journal Year: 2024, Volume and Issue: 633(8031), P. 848 - 855

Published: Aug. 14, 2024

Bread wheat (Triticum aestivum) is a globally dominant crop and major source of calories proteins for the human diet. Compared with its wild ancestors, modern bread shows lower genetic diversity, caused by polyploidisation, domestication breeding bottlenecks

Language: Английский

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Pan-genome bridges wheat structural variations with habitat and breeding

Nature, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 27, 2024

Language: Английский

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FIND-IT: Accelerated trait development for a green evolution

Science Advances, Journal Year: 2022, Volume and Issue: 8(34)

Published: Aug. 24, 2022

Improved agricultural and industrial production organisms are required to meet the future global food demands minimize effects of climate change. A new resource for crop microbe improvement, designated FIND-IT (Fast Identification Nucleotide variants by droplet DigITal PCR), provides ultrafast identification isolation predetermined, targeted genetic in a screening cycle less than 10 days. Using large-scale sample pooling combination with digital PCR (ddPCR) greatly increases size low–mutation density screenable variant libraries probability identifying interest. The method is validated totaling 500,000 barley ( Hordeum vulgare ) individuals isolating more 125 gene knockout lines miRNA or promoter enabling functional analysis. directly applicable elite breeding pipelines time-consuming technical steps accelerate evolution germplasm.

Language: Английский

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Two functional CC‐NBS‐LRR proteins from rye chromosome 6RS confer differential age‐related powdery mildew resistance to wheat

Plant Biotechnology Journal, Journal Year: 2023, Volume and Issue: 22(1), P. 66 - 81

Published: Aug. 24, 2023

Summary Rye ( Secale cereale ), a valuable relative of wheat, contains abundant powdery mildew resistance Pm ) genes. Using physical mapping, transcriptome sequencing, barley stripe mosaic virus‐induced gene silencing, ethyl methane sulfonate mutagenesis, and stable transformation, we isolated validated two coiled‐coil, nucleotide‐binding site leucine‐rich repeat (CC‐NBS‐LRR) alleles, PmTR1 PmTR3 , located on rye chromosome 6RS from different triticale lines. confers age‐related starting the three‐leaf stage, whereas its allele, typical all‐stage resistance, which may be associated with their differential expression patterns. Overexpression in Nicotiana benthamiana showed that CC, CC‐NBS, CC‐LRR fragments PMTR1 induce cell death, PMTR3 CC full‐length perform this function. Luciferase complementation imaging pull‐down assays revealed distinct interaction activities between NBS fragments. Our study elucidates novel rye‐derived genes derivative germplasm resources provides insights into mechanism can aid improvement against wheat mildew.

Language: Английский

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Smart breeding driven by advances in sequencing technology

Modern Agriculture, Journal Year: 2023, Volume and Issue: 1(1), P. 43 - 56

Published: March 1, 2023

Abstract Crop breeding has undergone numerous advancements throughout history with the next revolution being uncertain. This review provides an overview of techniques from traditional selective and crossbreeding to modern based on molecular marker‐assisted genomic selection. Systematic analysis mainstream genotyping approaches principle, application scenario supporting software revealed that changes in technology have led explosion data. data expansion will drive a breakthrough sequencing integrate artificial intelligence automation. The also discusses technological associated during big era, including models, technologies future intelligent breeding, provide references for crop breeders. paper highlights potential smart driven by advances technology, which lead development new strategies accelerate process.

Language: Английский

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