EIL (ethylene‐insensitive 3‐like) transcription factors in apple affect both ethylene‐ and cold response‐dependent fruit ripening DOI Creative Commons
Jiao Feng, Mindy Wang, Xiuyin Chen

et al.

The Plant Journal, Journal Year: 2025, Volume and Issue: 121(5)

Published: March 1, 2025

EIN3/EIL (ethylene-insensitive 3/EIN3-like) transcription factors are positive downstream transcriptional regulators of ethylene signalling. In apple (Malus × domestica), a small family MdEIL genes was identified, with four expressed in fruit. Transgenic lines were generated to manipulate MdEIL1 expression, and fruits sampled at harvest maturity after cold treatment. Their fruit ripening behaviour compared control contrasted ACC OXIDASE 1 antisense line (ACO1as) which produced no associated ethylene. Two transgenic showed strong co-suppression MdEIL1-4 expression as well reduced production, softening aroma while one overexpressing enhanced ripening. Key involved biosynthesis ethylene-dependent cell wall modification (MdXTH1, MdβGAL) (MdAFS1, MdoOMT1) downregulated the co-suppressed lines. Co-suppressed softening/volatile production treatment contrast ACO1as line, response-dependent (MdCBF2, dehydrins MdDHN2, -14, -16 MdNAC29a) remained cold-repressed. The action MdEILs shown using dual-luciferase reporter assays occur through direct activation MdAFS1, MdXTH1 MdβGAL promoters. Exogenous unable further stimulate promoter activation, but could. Promoter deletion analysis identified potential EIL binding sites MdAFS1 promoters electrophoretic mobility shift that MdEIL1-3 could all bind 32 bp fragment promoter. Together these results indicate contribute suite attributes via an manner, also response cold.

Language: Английский

Research progress on the physiological response and molecular mechanism of cold response in plants DOI Creative Commons
Yong Wang,

Jin Wang,

Rehman Sarwar

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Jan. 30, 2024

Low temperature is a critical environmental stress factor that restricts crop growth and geographical distribution, significantly impacting quality yield. When plants are exposed to low temperatures, series of changes occur in their external morphology internal physiological biochemical metabolism. This article comprehensively reviews the alterations regulatory mechanisms indices, such as membrane system stability, redox system, fatty acid content, photosynthesis, osmoregulatory substances, response low-temperature plants. Furthermore, we summarize recent research on signal transduction pathways, phytohormones, epigenetic modifications, other molecular mediating temperatures higher In addition, outline cultivation practices improve plant cold resistance highlight cold-related genes used breeding. Last, discuss future directions, potential application prospects breeding, significant breakthroughs mechanisms.

Language: Английский

Citations

38
IbMYC2 Contributes to Salt and Drought Stress Tolerance via Modulating Anthocyanin Accumulation and ROS-Scavenging System in Sweet Potato DOI Open Access

Yuanfeng Hu,

Hongyuan Zhao, Luyao Xue

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(4), P. 2096 - 2096

Published: Feb. 8, 2024

Basic helix–loop–helix (bHLH) transcription factors extensively affect various physiological processes in plant metabolism, growth, and abiotic stress. However, the regulation mechanism of bHLH balancing anthocyanin biosynthesis stress sweet potato (Ipomoea batata (L.) Lam.) remains unclear. Previously, transcriptome analysis revealed genes that were differentially expressed among purple-fleshed cultivar ‘Jingshu 6’ its anthocyanin-rich mutant ‘JS6-5’. Here, we selected one these potential genes, IbMYC2, which belongs to factor family, for subsequent analyses. The expression IbMYC2 JS6-5 storage roots is almost four-fold higher than Jingshu 6 significantly induced by hydrogen peroxide (H2O2), methyl jasmonate (MeJA), NaCl, polyethylene glycol (PEG)6000. Overexpression enhances production exhibits a certain antioxidant capacity, thereby improving salt drought tolerance. In contrast, reducing increases susceptibility. Our data showed could elevate synthesis pathway binding IbCHI IbDFR promoters. Additionally, overexpressing activates encoding reactive oxygen species (ROS)-scavenging proline enzymes under conditions. Taken together, results demonstrate gene exercises significant impact on crop quality resistance.

Language: Английский

Citations

19
Multiple roles of NAC transcription factors in plant development and stress responses DOI Open Access
Haiyan Xiong,

Haidong He,

Yu Chang

et al.

Journal of Integrative Plant Biology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 14, 2025

ABSTRACT NAC (NAM, ATAF1/2, and CUC2) transcription factors (TFs) are a family of plant‐specific TFs that play crucial roles in various aspects plant development stress responses. Here, we provide an in‐depth review the structural characteristics, regulatory mechanisms, functional NACs different species. One key features is their ability to regulate gene expression through variety including binding DNA sequences promoter regions target genes, interacting with other TFs, modulating chromatin structure. We discuss these mechanisms detail, providing insights into complex networks govern activity NACs. explore diverse functions growth processes, embryogenesis, seed development, root shoot floral fruit ripening, secondary cell wall formation, senescence. also response stresses, drought, flooding, heat, cold, salinity, nutrient deficit, diseases. Lastly, emphasize crosstalk role between developmental processes This integrated perspective highlights how orchestrate resilience. Overall, this provides comprehensive overview pivotal responses, emphasizing potential for engineering stress‐resistant crops enhancing agricultural productivity.

Language: Английский

Citations

8
A novel R2R3-MYB transcription factor PbMYB1L of Pyrus bretschneideri regulates cold tolerance and anthocyanin accumulation DOI
Xuan Zhou,

Diya Lei,

Wantian Yao

et al.

Plant Cell Reports, Journal Year: 2024, Volume and Issue: 43(2)

Published: Jan. 11, 2024

Language: Английский

Citations

14
The miR6445‐NAC029 module regulates drought tolerance by regulating the expression of glutathione S‐transferase U23 and reactive oxygen species scavenging in Populus DOI

Meng‐Xue Niu,

Cong‐Hua Feng,

Fang He

et al.

New Phytologist, Journal Year: 2024, Volume and Issue: 242(5), P. 2043 - 2058

Published: March 21, 2024

MicroRNAs are essential in plant development and stress resistance, but their specific roles drought require further investigation. Here, we have uncovered that a Populus-specific microRNAs (miRNA), miR6445, targeting NAC (NAM, ATAF, CUC) family genes, is involved regulating tolerance of poplar. The expression level miR6445 was significantly upregulated under stress; concomitantly, seven targeted genes showed significant downregulation. Silencing the by short tandem target mimic technology decreased Furthermore, 5' RACE experiments confirmed directly NAC029. overexpression lines PtrNAC029 (OE-NAC029) increased sensitivity to compared with knockout (Crispr-NAC029), consistent drought-sensitive phenotype observed miR6445-silenced strains. verified bind promoters glutathione S-transferase U23 (GSTU23) inhibit its expression. Both Crispr-NAC029 PtrGSTU23 overexpressing plants higher levels transcript GST activity while accumulating less reactive oxygen species (ROS). Moreover, poplars GSTU23 demonstrated enhanced tolerance. Taken together, our research reveals crucial role miR6445-NAC029-GSTU23 module enhancing poplar ROS homeostasis. This finding provides new molecular targets for improving resistance trees.

Language: Английский

Citations

10
MdbHLH160 is stabilized via reduced MdBT2-mediated degradation to promote MdSOD1 and MdDREB2A-like expression for apple drought tolerance DOI
Ke Mao, Jie Yang, Yunxia Sun

et al.

PLANT PHYSIOLOGY, Journal Year: 2023, Volume and Issue: 194(2), P. 1181 - 1203

Published: Oct. 31, 2023

Drought stress is a key environmental factor limiting the productivity, quality, and geographic distribution of crops worldwide. Abscisic acid (ABA) plays an important role in plant drought responses, but molecular mechanisms remain unclear. Here, we report ABA-responsive bHLH transcription factor, MdbHLH160, which promotes tolerance Arabidopsis (Arabidopsis thaliana) apple (Malus domestica). Under conditions, MdbHLH160 directly bound to MdSOD1 (superoxide dismutase 1) promoter activated its transcription, thereby triggering reactive oxygen species (ROS) scavenging enhancing tolerance. also promoted enzyme activity accumulation nucleus through direct protein interactions, thus inhibiting excessive nuclear ROS levels. Moreover, upregulated expression MdDREB2A-like, DREB (dehydration-responsive element binding factor) family gene that Protein degradation ubiquitination assays showed ABA treatment stabilized MdbHLH160. The BTB MdBT2 was identified as MdbHLH160-interacting degradation, substantially inhibited this process. Overall, our findings provide insights into ABA-modulated at both transcriptional post-translational levels via ABA-MdBT2-MdbHLH160-MdSOD1/MdDREB2A-like cascade.

Language: Английский

Citations

20
Transcriptomic Analysis Reveals the Flavonoid Biosynthesis Pathway Involved in Rhizome Development in Polygonatum cyrtonema Hua DOI Creative Commons
Kui Wan,

Jingjie Ban,

Fengjie Yang

et al.

Plants, Journal Year: 2024, Volume and Issue: 13(11), P. 1524 - 1524

Published: May 31, 2024

Polygonatum cyrtonema Hua (P. cyrtonema) rhizomes are rich in flavonoids and other secondary metabolites, exhibiting remarkable antioxidant, anti-tumor, immunomodulatory effects. flavonoid-biosynthesis-related genes have been characterized already. However, a comprehensive overview of flavonoid biosynthesis pathways is still absent. To articulate the accumulation pathways, we examined transcriptome changes using Illumina HiSeq from five different tissues RNA-seq 15 samples had over 105 Gb clean base, generating total 277,955 unigenes. The cDNA libraries fruits (F), leaves (L), roots (R), stems (S), (T) three-year-old P. plants generated 57,591, 53,578, 60,321, 51,530, 54,935 Comparative analysis revealed that 379 differentially expressed (DEGs) were group F _vs_ T, L R S transcripts DEGs principally enriched rhizomes. In addition, combined with WGCNA FPKM tissues’ transcription, nine transcription factor families (MYB, WRKY, AP2/ERF, etc.) red module, module positively correlated rhizome accumulation. Quantitative real-time PCR (qRT-PCR) further indicated BZIP1, C3H31, ERF114, DREB21 rhizomes, accompanied development cyrtonema. Therefore, this study provides foundation for research into uncovering

Language: Английский

Citations

7
A multi-omics approach reveals low temperature inhibition of flavones and flavonols accumulation in postharvest bananas via downregulation of MabHLH363 DOI

Wen Song,

Shu-Qin Lin,

Qi Yin

et al.

Postharvest Biology and Technology, Journal Year: 2024, Volume and Issue: 218, P. 113152 - 113152

Published: Aug. 20, 2024

Language: Английский

Citations

7
Understanding cold stress response mechanisms in plants: an overview DOI Creative Commons

Zhenfeng Qian,

Lilian He,

Fusheng Li

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Nov. 6, 2024

Low-temperature stress significantly impacts plant growth, development, yield, and geographical distribution. However, during the long-term process of evolution, plants have evolved complicated mechanisms to resist low-temperature stress. The cold tolerance trait is regulated by multiple pathways, such as Ca 2+ signaling cascade, mitogen-activated protein kinase (MAPK) inducer CBF expression 1 (ICE1)-C-repeat binding factor (CBF)-cold-reulated gene (COR) transcriptional reactive oxygen species (ROS) homeostasis regulation, hormone signaling. specific responses these pathways their interactions are not fully understood. This review summarizes response from four aspects, including signal perception transduction, ICE1-CBF-COR transcription cascade ROS regulation regulation. It also elucidates mechanism transduction in plants, proposes important roles factors (TFs), post-translational modifications (PTMs), light signals, circadian clock factors, interaction proteins cascade. Additionally, we analyze importance response, explore cross interconnections among homeostasis, comprehensive enhances our understanding provides a molecular basis for genetic strategies improve tolerance.

Language: Английский

Citations

7
A C2H2‐type zinc finger protein ZAT12 of Poncirus trifoliata acts downstream of CBF1 to regulate cold tolerance DOI
Yang Zhang, Wei Xiao, Min Wang

et al.

The Plant Journal, Journal Year: 2023, Volume and Issue: 117(5), P. 1317 - 1329

Published: Nov. 28, 2023

SUMMARY The Cys2/His2 (C2H2)‐type zinc finger family has been reported to regulate multiple aspects of plant development and abiotic stress response. However, the role C2H2‐type proteins in cold tolerance remains largely unclear. Through RNA‐sequence analysis, a cold‐responsive protein, named as PtrZAT12 , was identified isolated from trifoliate orange ( Poncirus trifoliata L. Raf.), cold‐hardy closely related citrus. Furthermore, we found that markedly induced by various stresses, especially stress. is nuclear physiological analysis suggests overexpression conferred enhanced transgenic tobacco Nicotiana tabacum ) plants, while knockdown virus‐induced gene silencing (VIGS) increased sensitivity repressed expression genes involved tolerance. promoter harbors DRE/CRT cis ‐acting element, which verified be specifically bound PtrCBF1 C‐repeat BINDING FACTOR1). VIGS‐mediated reduced relative levels decreased resistance orange. Based on these results, propose direct target CBF1 plays positive modulation knowledge gains new insight into regulatory module composed CBF1‐ ZAT12 response advances our understanding plants.

Language: Английский

Citations

14