Genome-wide analysis of C-repeat binding factor (CBF) family members in Pennisetum glaucum and the function of PgCBF21 under cold stress DOI
Wei Li,

Tian Li,

Ying Dong

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 1, 2024

Abstract Background Pennisetum hybrid (Pennisetum glaucum x Pennisetum purpureum), a perennial forage grass of the Poaceae family, it has well-developed root system, strong tillering ability, high biomass yield, and rich nutritional content, making premium grass. However, Pennisetum genera grasses are sensitive to low-temperature stress lacks ability adapt cold environments, severely limiting its cultivation in temperate high-altitude regions. Results We identified 23 PgCBF genes, unevenly distributed across chromosomes 1 7. Physicochemical property analysis revealed that amino acid count PgCBF family members ranged from 187 324, with molecular weights between 19688.36 34271.70 Da theoretical isoelectric points 4.57 9.82. Promoter region genes indicated presence various cis-acting elements involved hormone response, tissue-specific expression, response. Additionally, qRT-PCR results showed significant upregulation PgCBF11 expression stems following treatment, increasing over 40-fold after 32 hours treatment about 20-fold 8 treatment. PgCBF21 exhibited response leaves levels rising more than 37-fold. Furthermore, through overexpression PgCBF21, we ascertained capacity enhance tolerance yeast. Conclusion This study analyzed characteristics several candidate responsive stress, providing foundation for future research on innovative utilization cold-resistant resources.

Language: Английский

A complete reference genome assembly for foxtail millet and Setaria-db, a comprehensive database for Setaria DOI Creative Commons

Qiang He,

Chunchao Wang,

Qiang He

et al.

Molecular Plant, Journal Year: 2023, Volume and Issue: 17(2), P. 219 - 222

Published: Dec. 28, 2023

Language: Английский

Citations

18
Advance computational tools for multiomics data learning DOI
Sheikh Mansoor,

Saira Hamid,

Thai Thanh Tuan

et al.

Biotechnology Advances, Journal Year: 2024, Volume and Issue: 77, P. 108447 - 108447

Published: Sept. 7, 2024

Language: Английский

Citations

8
Genome-Wide Identification and Transcriptional Analysis of AP2/ERF Gene Family in Pearl Millet (Pennisetum glaucum) DOI Open Access
Liang Xu,

Ying Lan,

Miaohong Lin

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(5), P. 2470 - 2470

Published: Feb. 20, 2024

The apetala2/ethylene response factor (AP2/ERF) gene family plays a crucial role in regulating plant growth and development responding to different abiotic stresses (e.g., drought, heat, cold, salinity). However, the knowledge of ERF pearl millet remains limited. Here, total 167 high-confidence PgERF genes are identified divided into five subgroups based on gene-conserved structure phylogenetic analysis. Forty-one pairs segmental duplication found using collinear Nucleotide substitution analysis reveals these duplicated under positive purification, indicating they actively natural selection. Comprehensive transcriptomic that genesare preferentially expressed imbibed seeds stem (tilling stage) respond salt stress. Prediction cis-regulatory element by PlantCARE program indicates involved responses environmental stimuli. Using reverse transcription quantitative real-time PCR (RT-qPCR), expression profiles eleven selected monitored various tissues during stresses. Transcript levels each exhibit significant changes stress treatments. Notably, PgERF7 is only candidate can be induced all adverse conditions. Furthermore, four (i.e., PgERF22, PgERF37, PgERF88, PgERF155) shown ABA-dependent signaling pathway. These results provide useful bioinformatic transcriptional information for understanding roles adaptation climate change.

Language: Английский

Citations

7
The evolution and expansion of RWP-RK gene family improve the heat adaptability of elephant grass (Pennisetum purpureum Schum.) DOI Creative Commons

Yarong Jin,

Jinchan Luo,

Yuchen Yang

et al.

BMC Genomics, Journal Year: 2023, Volume and Issue: 24(1)

Published: Aug. 31, 2023

Along with global warming, resulting in crop production, exacerbating the food crisis. Therefore, it is urgent to study mechanism of plant heat resistance. However, resistance genes were lost due long-term artificial domestication. By analyzing potential tolerance and molecular mechanisms other wild materials, more genetic resources can be provided for improving crops. Elephant grass (Pennisetum purpureum Schum.) has strong adaptability stress contains abundant heat-resistant gene resources.Through sequence structure analysis, a total 36 RWP-RK members identified elephant grass. Functional analysis revealed their close association stress. Four randomly selected RKDs (RKD1.1, RKD4.3, RKD6.6, RKD8.1) analyzed expression, results showed upregulation under high temperature conditions, suggesting active role response The family (36 genes) 2.4 times higher than that related tropical crops, rice (15 sorghum genes). RWPs contain 15 NLPs 21 RKDs, 73% are WGD. Among them, combined DAP-seq results, was found expansion could improve by enhancing nitrogen use efficiency peroxidase expression.RWP-RK closely thermal adaptation evolution speciation. RKD subgroup responsiveness NLP when exposed promoter region significant number MeJA ABA responsive elements, which may contribute positive These scientific basis

Language: Английский

Citations

12
Gene editing tool kit in millets: present status and future directions DOI

Vidhi Sapara,

Mitesh Khisti,

Kalenahalli Yogendra

et al.

The Nucleus, Journal Year: 2024, Volume and Issue: 67(1), P. 157 - 179

Published: April 1, 2024

Language: Английский

Citations

4
Genomic selection for agronomical phenotypes using genome-wide SNPs and SVs in pearl millet DOI
Haidong Yan,

Yarong Jin,

Haipeng Yu

et al.

Theoretical and Applied Genetics, Journal Year: 2024, Volume and Issue: 137(10)

Published: Oct. 1, 2024

Language: Английский

Citations

4
Utilizing modern techniques and omics technologies to enhance stress tolerance in finger millet, with recent findings: A review DOI Creative Commons
D. Yuvaraj,

J. Bevin Nishanth,

A. Premkumar

et al.

Food Chemistry Advances, Journal Year: 2025, Volume and Issue: 6, P. 100886 - 100886

Published: Jan. 11, 2025

Language: Английский

Citations

0
Development of Genome-Wide SSR Markers in Leymus chinensis with Genetic Diversity Analysis and DNA Fingerprints DOI Open Access

Taiyou Ou,

Zinian Wu,

Chunyu Tian

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(3), P. 918 - 918

Published: Jan. 22, 2025

Leymus chinensis, a major component of the plant community in eastern Eurasian grasslands with wide distribution, provides stability to grassland ecosystems and supports animal husbandry. This study aimed bridge gap between molecular breeding industrial application L. chinensis by conducting comprehensive simple sequence repeat (SSR) analysis. A total 973,129 SSRs were identified whole genome, which was used design 20 polymorphic pairs SSR primers further assess 105 accessions. On average, 33.55 alleles detected per locus, an average Shannon index 2.939 information content value 0.910. Principal coordinate, maximum likelihood, structure analyses consistently showed that all samples coincidentally divided into four subclasses. In addition, Mantel test data indicated weak correlation genetic geographical distances whose variability may be related pollination mode natural selection pressures. Finally, we selected markers scan accessions, constructing fingerprint for them. These findings provide new foundations identifying superior varieties, improving management resources, germplasm resource database chinensis.

Language: Английский

Citations

0
Integrated transcriptome and metabolome reveal hydroxypyruvate reductase DgHPR1 positively regulates flowering time in orchardgrass DOI

Miaoli Wang,

Guangyan Feng,

Feixiang Hao

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 305, P. 141164 - 141164

Published: Feb. 16, 2025

Language: Английский

Citations

0
The conservative chloroplast genomic features and comparative analysis provide new evidence for the unification of Pennisetum and Cenchrus DOI Creative Commons
Bingchao Wu,

Jiyuan Jia,

Dan Luo

et al.

Industrial Crops and Products, Journal Year: 2025, Volume and Issue: 226, P. 120747 - 120747

Published: Feb. 20, 2025

Language: Английский

Citations

0