Phage Display Screening of Anchor Peptides for Red Blood Cell-Derived Extracellular Vesicles DOI Creative Commons
Limei Xu,

Xiao Xu,

Jiang Xia

et al.

ACS Omega, Journal Year: 2024, Volume and Issue: 9(6), P. 6492 - 6504

Published: Jan. 31, 2024

Extracellular vesicles (EVs) are increasingly used for disease diagnosis and treatment. Among them, red blood cell-derived EVs (RBC-EVs) have attracted great attention due to their abundant sources low risks of gene transfer (RBC-EVs lack nuclear mitochondrial DNA). Here, we first revealed the high expression level membrane protein solute carrier family 4 member 1 (SLC4A1) in RBC-EVs through proteomic analysis. We then identified several binding peptides with affinity SLC4A1 extracellular domain (SLC4A1-EC) from phage display library screening. A SLC4A1-EC three (XRB2, XRE4, XRH7) were assessed vitro using surface plasmon resonance analysis SDS–polyacrylamide gel electrophoresis (SDS–PAGE). The sites polypeptides further predicted by LigPlot + analysis, results showed that these could bind part hydrophobic residues SLC4A1-EC. efficiency anchor was verified flow cytometry fluorescence imaging. In conclusion, successfully screened specific RBC-EV-targeting which potentially be utilized isolating RBC-derived serum samples. More importantly, this peptide coupled targeting modify drug delivery. Our work will provide a viable method optimizing function RBC-EVs.

Language: Английский

Extracellular vesicles: Function, resilience, biomarker, bioengineering, and clinical implications DOI Creative Commons
Der‐Shan Sun, Hsin‐Hou Chang

Tzu Chi Medical Journal, Journal Year: 2024, Volume and Issue: unknown

Published: May 24, 2024

Extracellular vesicles (EVs) have emerged as key players in intercellular communication, disease pathology, and therapeutic innovation. Initially overlooked cellular debris, EVs are now recognized vital mediators of cell-to-cell ferrying a cargo proteins, nucleic acids, lipids, providing resilience response to stresses. This review provides comprehensive overview EVs, focusing on their role biomarkers diagnosis, functional significance physiological pathological processes, the potential bioengineering for applications. offer promising avenue noninvasive diagnosis monitoring, reflecting state originating cells. Their diagnostic spans spectrum diseases, including cancer, cardiovascular disorders, neurodegenerative infectious diseases. Moreover, presence bodily fluids such blood, urine, cerebrospinal fluid enhances utility, presenting advantages over traditional methods. Beyond diagnostics, mediate crucial roles facilitating transfer bioactive molecules between communication modulates various processes tissue regeneration, immune modulation, neuronal communication. Dysregulation EV-mediated is implicated diseases highlighting potential. Bioengineering techniques avenues manipulating applications, from isolation purification engineering targeted delivery systems. These approaches hold promise developing novel therapeutics tailored specific revolutionizing personalized medicine. However, challenges standardization, scalability, regulatory approval need addressing successful clinical translation. Overall, represent dynamic frontier biomedical research with vast therapeutics,

Language: Английский

Citations

1
The researcher's guide to selecting biomarkers in mental health studies DOI Creative Commons
Josine E. Verhoeven, Owen M. Wolkowitz,

Isaac Barr Satz

et al.

BioEssays, Journal Year: 2024, Volume and Issue: 46(10)

Published: Sept. 11, 2024

Abstract Clinical mental health researchers may understandably struggle with how to incorporate biological assessments in clinical research. The options are numerous and described a vast complex body of literature. Here we provide guidelines assist seeking include measures their studies. Apart from focus on behavioral outcomes as measured via interviews or questionnaires, advocate for pathways trials epidemiological studies that help clarify pathophysiology mechanisms action, delineate subgroups participants, mediate treatment effects, inform personalized strategies. With this paper aim bridge the gap between research by (1) discussing relevance, measurement reliability, feasibility relevant peripheral biomarkers; (2) addressing five types tissues, namely blood, saliva, urine, stool hair; (3) providing information control sources variability.

Language: Английский

Citations

1
Exosomes as a window of opportunity in hepatocellular carcinoma: Insights from diagnostic to targeted drug delivery DOI
Supriya Gupta,

A. Deb,

Pranab Behari Mazumder

et al.

Journal of Drug Delivery Science and Technology, Journal Year: 2024, Volume and Issue: 101, P. 106259 - 106259

Published: Oct. 9, 2024

Language: Английский

Citations

1
Toxoplasma gondii infection of neurons alters the production and content of extracellular vesicles altering astrocyte phenotype and contributing to the loss of GLT-1 in the infected brain DOI Creative Commons
Emily Tabaie, Ziting Gao,

Stacey Gomez

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 8, 2024

Abstract Toxoplasma gondii (T. gondii) cyst formation in the central nervous system only occurs neurons allowing parasite to remain latent for lifetime of host. Astrocytes are fundamental neuronal health by providing nutrients and structural support help regulate neurotransmitters continuous communication with neurons. It is not yet known how infection presence intracellular cysts, disrupts crucial relationship between these cells. Extracellular vesicles (EVs) function can contain proteins, lipids, DNA, miRNA, other RNA subtypes. EVs produced all cells including play an important role neuronal-astrocyte interactions regulation glutamate receptors on astrocytes. Previous work has demonstrated reduces astrocytic expression primary transporter, GLT-1. Here we tested if alters production content EVs. were isolated from uninfected infected murine cortical their size, concentration, characterization confirmed nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM), CD63 ELISA, liquid chromatography (LC)-mass spectrometry (MS)/MS, microRNA Sequencing. Analysis reveals that reduced altered protein miRNA content. contained secreted proteins GRA1, GRA2, GRA7, MAG1 MAG2 associated formation. Following incubation astrocytes, a proportion colocalize nucleus. gene astrocytes leading downregulation GLT-1 increase pro-inflammatory transcriptional signatures. These results demonstrate ability parasitic brain alter EV

Language: Английский

Citations

1
Phage Display Screening of Anchor Peptides for Red Blood Cell-Derived Extracellular Vesicles DOI Creative Commons
Limei Xu,

Xiao Xu,

Jiang Xia

et al.

ACS Omega, Journal Year: 2024, Volume and Issue: 9(6), P. 6492 - 6504

Published: Jan. 31, 2024

Extracellular vesicles (EVs) are increasingly used for disease diagnosis and treatment. Among them, red blood cell-derived EVs (RBC-EVs) have attracted great attention due to their abundant sources low risks of gene transfer (RBC-EVs lack nuclear mitochondrial DNA). Here, we first revealed the high expression level membrane protein solute carrier family 4 member 1 (SLC4A1) in RBC-EVs through proteomic analysis. We then identified several binding peptides with affinity SLC4A1 extracellular domain (SLC4A1-EC) from phage display library screening. A SLC4A1-EC three (XRB2, XRE4, XRH7) were assessed vitro using surface plasmon resonance analysis SDS–polyacrylamide gel electrophoresis (SDS–PAGE). The sites polypeptides further predicted by LigPlot + analysis, results showed that these could bind part hydrophobic residues SLC4A1-EC. efficiency anchor was verified flow cytometry fluorescence imaging. In conclusion, successfully screened specific RBC-EV-targeting which potentially be utilized isolating RBC-derived serum samples. More importantly, this peptide coupled targeting modify drug delivery. Our work will provide a viable method optimizing function RBC-EVs.

Language: Английский

Citations

1