Relationship between Fertility Traits and Kinematics in Clusters of Boar Ejaculates

Biology, Journal Year: 2021, Volume and Issue: 10(7), P. 595 - 595

Published: June 28, 2021

The aim was to determine the relationship between kinematic parameters of boar spermatozoa and fertility rates sow, as well assess effect sperm clusters on capacity ejaculate. Semen samples were collected from 11 sexually mature boars. Samples analyzed by an ISAS®v1 CASA-Mot system for eight parameters. Ejaculate characterized using multivariate procedures, such principal factors (PFs) analysis clustering methods (the k-means model). Four different ejaculate identified two PFs which involved linear trajectory velocity. There differences (p < 0.05) variables sire line. no statistical difference > dam lines in variables. discriminant ability kinematics predict litter size receiver operating characteristics (ROC) curve analysis. Curvilinear velocity (VCL), average path (VAP), amplitude lateral head displacement (ALH), beat-cross frequency (BCF) showed significant, albeit limited, predictive (range: 0.55–0.58 area under curve, AUC). ejaculates did not have a

Language: Английский

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Kinematic and head morphometric characterisation of spermatozoa from the Brown Caiman (Caiman crocodilus fuscus)

Animal Reproduction Science, Journal Year: 2019, Volume and Issue: 207, P. 9 - 20

Published: June 12, 2019

Language: Английский

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Sperm kinematics and morphometric subpopulations analysis with CASA systems: a review

Revista de Biología Tropical, Journal Year: 2019, Volume and Issue: 67(6)

Published: Nov. 6, 2019

Sperm kinematics and morphometric subpopulations analysis with CASA systems: a review. The subjective evaluation of seminal quality has given way to the use objective assessment techniques by technology (computer-assisted semen analysis). application principal components (PC) clustering methods reveal spermatozoa is powerful tool evaluate raw processed cell suspensions, but not many researchers are aware technique. PC multivariate statistical method that reduces number variables used in subsequent calculations describe data. By integrating original according their coherence database into new complex mathematical variable, clearly defined homogenous can be defined. Kinematic, morphometric, morphological or DNA integrity tests may apply characterize it understand reproductive biology spermatozoon. In recent years there been substantive change conceptual paradigm regarding what an ejaculate is. It should said until recently was considered representative population (billions) sperm made up "equivalent" cells common goal: one could finally fertilize oocyte. verification set grouped distinct subpopulations, kinematic characteristics, opens towards more cooperative vision. Besides, we have seen subpopulation distribution different among males, which seems indicate strategies understood within another paradigm, competition between ejaculates. existence heterogeneous show patterns widely known biological meaning these still clear. Although role remains unknown, work continue direction.

Language: Английский

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Relationships between Biomarkers of Oxidative Stress in Seminal Plasma and Sperm Motility in Bulls before and after Cryopreservation

Animals, Journal Year: 2022, Volume and Issue: 12(19), P. 2534 - 2534

Published: Sept. 22, 2022

This study aimed at evaluating the relationship between biomarkers of oxidative stress (OS) in seminal plasma and sperm motility bulls before after cryopreservation. Three ejaculates per bull were collected from 20 young bulls. Each ejaculate was analyzed for cryopreservation (by CASA), SP concentration Advanced Oxidation Protein Products (AOPP), thiols, carbonyl groups (CT) examined. Then, based on their motility, grouped into: high fresh (HMF), low (LMF), thawed (HMT), (LMT) groups. Higher AOPP thiol concentrations related (p < 0.05) to higher LIN BCF lower ALH semen. In addition, thiols significantly HMF than LMF. As a confirmation this, Receiver Operating Characteristic (ROC) curve analysis showed that able discriminate LMF (Area Under Curve 71.67% 72.04%, respectively). These observations give an alternative perspective OS parameters SP, which need further investigations.

Language: Английский

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Effect of age, season, breed composition, and sperm counting chamber on boar semen quality in tropics

Published: Aug. 21, 2024

Understanding the factors affecting semen quality is important for optimizing male reproductive efficiency. The present study aimed to evaluate seminal associated with breed, age, season, and sperm counting chamber in boar ejaculates. A total of 22 sexually mature healthy boars from maternal sire commercial breeds were utilized as donors, an average age (mean ± standard deviation) 21.0 7.2 months. Boars housed individually well-ventilated pens fed a breeder mix. Semen samples collected each dry rainy season. grouped according into three categories: &amp;lt;12 months, 12-24 &amp;gt;24 Only ejaculates at least 75 % morphologically normal more than 8.5 x 109 per ejaculate used. analyzed using Computer-Assisted Analysis (CASA) ISAS®v1 system. Results showed that younger (&amp;lt;12 months) exhibited higher progressive motility faster swimming patterns compared older boars. percentages fast spermatozoa increased by kinematics variables significant differences (P&amp;lt;0.05) between chambers. Duroc Landrace presented greater motility, while terminal line accelerated linear progressiveness (P&amp;lt;0.05). Overall, impact various on kinematic ejaculates, including genetic breed composition, enables better understand This highlights importance swine management practices active

Language: Английский

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A New Approach of Sperm Motility Subpopulation Structure in Donkey and Horse

Frontiers in Veterinary Science, Journal Year: 2021, Volume and Issue: 8

Published: May 25, 2021

This study aimed to characterize the sperm kinematic values with high frames per second, define subpopulation structure of a horse and donkey compare them. A total 57 fresh semen ejaculates (26 Spanish 16 Arabian breeds 10 donkeys) were collected subsequently analyzed for parameters using Computer-aided motility analysis ISAS®v1.2 system Spermtrack® 10-μm depth counting chamber. Sequences recorded at 250 eight automatically evaluated. All showed significant differences between breeds. evaluated excellent characteristics, significantly higher all donkeys compared horses except beat-cross frequency. Donkey was faster linear than horse. Regarding differences, had average path velocity, curvilinear frequency rapid, but Arab more linear. The principal component three subpopulations in ejaculate different characteristic dominant both straight, beat (38.2 41.7%, respectively), whereas lowest slowest non-linear sperms. This, plus slight distribution these horses, found. In conclusion, permitted have new interpretation motile species breed differences. More so, future works on spermatozoa should take into account that may interfere alter real performed.

Language: Английский

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Assessment of Chilling Injury in Boar Spermatozoa by Kinematic Patterns and Competitive Sperm-Oviduct Binding In Vitro

Animals, Journal Year: 2022, Volume and Issue: 12(6), P. 712 - 712

Published: March 11, 2022

Sensitive detection of chilling injury in boar spermatozoa is required to evaluate novel hypothermic preservation concepts. The study’s aim was examine whether analyses motility patterns and sperm binding a competitive oviduct explant assay (cOEA) sensitively detect chilling-induced alterations function. Semen samples (n = seven boars) were split into four subsamples by dilution either Beltsville Thawing Solution (BTS) or Androstar® Plus stored at 5 °C 17 °C. Storage temperature had significant effect on the distribution major kinematic clusters. size as estimated Cramer’s V higher (p < 0.05) BTS medium (0.21) compared AndroStar® (0.11). Spermatozoa extended relative capacity 0.05). Binding indices correlated with percentage viable, acrosome-intact (r 0.62) motile 0.72, both p 0.001). cluster slow, vigorous movement negatively sperm-oviduct −0.43, In conclusion, analysis kinematics vitro present meaningful biological tests assess concepts for semen preservation.

Language: Английский

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Influence of sperm cryopreservation on sperm motility and proAKAP4 concentration in mice

Reproductive Medicine and Biology, Journal Year: 2022, Volume and Issue: 21(1)

Published: Jan. 1, 2022

The protein proAKAP4 is crucial for sperm motility and has been suggested as an indicator of male fertility. We determined the relationship between concentration parameters in mice, investigated effects cryopreservation on these variables.Computer-assisted analysis ELISA were applied to determine fresh frozen-thawed epididymal SWISS, B6D2F1, C57BL/6N, BALB/c mice.ProAKAP4 levels ranged 12 89 ng/ml did not differ samples, or strains. found a negative some parameters. Sperm traits differed strains, negatively affected velocity but direction parameters.ProAKAP4 mouse spermatozoa unaffected by cryopreservation, highlighting robustness this parameter potentially time-independent marker high individual variation supports potential role quality, though we no positive, even relationships Future studies have investigate significance fertility mice.

Language: Английский

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Optimal frame rate when there were stallion sperm motility evaluations and determinations for kinematic variables using CASA-Mot analysis in different counting chambers

Animal Reproduction Science, Journal Year: 2020, Volume and Issue: 223, P. 106643 - 106643

Published: Nov. 2, 2020

Language: Английский

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Sperm kinematic subpopulations of the American crocodile (Crocodylus acutus)

PLoS ONE, Journal Year: 2021, Volume and Issue: 16(3), P. e0248270 - e0248270

Published: March 9, 2021

There has been very limited use of computer assisted semen analysis (CASA) to evaluate reptile sperm. The aim this study was examine sperm kinematic variables in American crocodile ( Crocodylus acutus ) samples and assess whether subpopulations could be characterized. Eight ejaculates (two ejaculates/male) from four sexually mature captive crocodiles were obtained. An ISAS®v1 CASA-Mot system, with an image acquisition rate 50 Hz, ISAS®D4C20 counting chambers used for analyses. percentages motile progressively spermatozoa did not differ among animals (P > 0.05) but there a significant animal effect regards < 0.05). Principal component (PC) revealed that grouped into three components: PC1, related velocity; PC2 progressiveness PC3 oscillation. Subpopulation structure identified groups 0.05), which represented, on average, 9.8%, 32.1%, 26.8%, 31.3% the total population. Males differed proportion each subpopulations. This new approach subpopulations, reflecting quantifiable parameters generated by CASA system technology, opens up possibilities future assessments will useful development reproduction these species.

Language: Английский

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