Methods in enzymology on CD-ROM/Methods in enzymology, Journal Year: 2022, Volume and Issue: unknown, P. 279 - 303
Published: Jan. 1, 2022
Language: Английский
Plants, Journal Year: 2023, Volume and Issue: 12(2), P. 269 - 269
Published: Jan. 6, 2023
Plants undergo metabolic perturbations under various abiotic stress conditions; due to their sessile nature, the network of plants requires continuous reconfigurations in response environmental stimuli maintain homeostasis and combat stress. The comprehensive analysis these features will thus give an overview plant responses strategies applied mitigate deleterious effects conditions at a biochemical level. In recent years, adoption metabolomics studies has gained significant attention growing technological advances analytical biochemistry (plant metabolomics). complexity landscape sophisticated, advanced methods. As such, advancements field have been realized, aided much by development refinement separatory techniques, including liquid gas chromatography (LC GC), often hyphenated state-of-the-art detection instruments such as mass spectrometry (MS) or nuclear resonance magnetic (NMR) spectroscopy. Significant developments techniques are briefly highlighted this review. enormous progress made far also comes with dawn Internet Things (IoT) technology housed machine learning (ML)-based computational tools for data acquisition, mining, 4IR era allowing broader coverage biological interpretation cellular status varying conditions. Thus, scientists can paint holistic roadmap predictive models metabolite-guided crop improvement. current review outlines application related elucidating stress, mainly focusing on heavy metal toxicity subsequent osmotic tolerance.
Language: Английский
Citations
32
The Plant Journal, Journal Year: 2025, Volume and Issue: 121(3)
Published: Feb. 1, 2025
SUMMARY Seeds of Brassicaceae produce a large diversity beneficial and antinutritional specialized metabolites (SMs) that influence their quality provide resistance to stresses. While SM distribution has been described in leaves root tissues, limited information is available about spatiotemporal accumulation seeds. Camelina sativa (camelina) an oilseed cultivated for human animal nutrition industrial uses. we previously explored plasticity, no expression related proteins genes camelina In this study, used multi‐omic approach, integrating untargeted metabolomics, proteomics, transcriptomics investigate the synthesis, modification, degradation SMs accumulated seed tissues (seed coat, endosperm, embryo) at six developmental two germination stages. Metabolomic results showed distinct patterns pathways, highlighting significant contrasts composition spatial defense‐related glucosinolate (GSL) compounds among camelina, Arabidopsis thaliana , Brassica napus three closely species. Notably, thanks metabolomic proteomic/transcriptomic techniques variation GSL distributions was primarily driven by differences structure (metabolomics data) transport (transcriptomic proteomic mechanisms. Long‐chain C8–C11 methylsulfinylalkyl GSLs were predominantly coat while mid‐ short‐chain C3–C7 embryo. Characterizing dynamics provides valuable insights can guide development crops with optimized toxic metabolites, improving nutritional profiles.
Language: Английский
Citations
1
Natural Product Reports, Journal Year: 2024, Volume and Issue: 41(5), P. 834 - 859
Published: Jan. 1, 2024
Diversity, functions and related enzymes of Specialized Metabolite (SM) modifications from plants seeds Brassicaceae are summarized in this review. A particular focus is made on SM plasticity modification seeds.
Language: Английский
Citations
7
Industrial Crops and Products, Journal Year: 2022, Volume and Issue: 182, P. 114944 - 114944
Published: April 16, 2022
Language: Английский
Citations
23
Plant Biotechnology Journal, Journal Year: 2022, Volume and Issue: 21(1), P. 189 - 201
Published: Sept. 27, 2022
Camelina sativa is an oil crop with low input costs and resistance to abiotic biotic stresses. The presence of glucosinolates, plant metabolites adverse health effects, restricts the use camelina for human animal nutrition. Cas9 endonuclease-based targeted mutagenesis three homeologs each glucosinolate transporters CsGTR1 CsGTR2 caused a strong decrease in amounts, highlighting power this approach inactivating multiple genes hexaploid crop. Mutagenesis transcription factors CsMYB28 CsMYB29 resulted complete loss representing first glucosinolate-free Brassicaceae protein contents fatty acid composition csgtr1csgtr2 csmyb28csmyb29 mutant seeds were not affected. elimination glucosinolates improves quality press cake camelina, which thus complies international standards regulating levels consumption feeding.
Language: Английский
Citations
22
Comptes Rendus Biologies, Journal Year: 2023, Volume and Issue: 345(4), P. 61 - 110
Published: Jan. 10, 2023
Elucidating the mechanisms that control seed development, metabolism, and physiology is a fundamental issue in biology. Michel Caboche had long been catalyst for biology research France up until his untimely passing away last year. To honour memory, we have updated review written under coordination 2010 entitled “Arabidopsis secrets unravelled after decade of genetic omics-driven research”. This encompassed different molecular aspects reserve accumulation, dormancy germination, are studied lab created by M. Caboche. We extended scope this to highlight original experimental approaches implemented field over past such as omics aimed at investigating gene expression, protein modifications, primary specialized metabolites tissue or even cellular level, well biodiversity impact environment on quality. L’élucidation des mécanismes qui contrôlent le développement, métabolisme et la physiologie graines est une question fondamentale en biologie. longtemps été un catalyseur de recherche biologie jusqu’à son décès prématuré l’année dernière. Pour honorer sa mémoire, nous avons mis à jour revue écrite sous intitulée « Arabidopsis ». Cette englobait différents moléculaires du développement graines, l’accumulation réserves, dormance sont étudiés dans laboratoire créé par Nous étendu portée cette pour mettre évidence approches expérimentales originales mises œuvre domaine au cours dernière décennie, telles que les omiques visant étudier contrôle l’expression gènes, modifications protéines, métabolites primaires spécialisés niveau tissus ou même cellules, tout tenant compte biodiversité l’impact l’environnement sur leur qualité.
Citations
12
PLANT PHYSIOLOGY, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 15, 2025
Abstract The emerging crop Camelina sativa (L.) Crantz (camelina) is a Brassicaceae oilseed with rapidly growing reputation for the deployment of advanced lipid biotechnology and metabolic engineering. recognised by agronomists its traits including yield, oil/protein content, drought tolerance, limited input requirements, plasticity resilience. Its utility as platform engineering was then quickly recognised, biotechnologists have benefited from short life cycle facile genetic transformation, producing numerous transgenic interventions to modify seed content generate novel products. desire work plant that both model has driven expansion research resources camelina, increased availability genome other “-omics” data sets. Collectively these established camelina an ideal study regulation metabolism improvement. Furthermore, unique characteristics enables design-build-test-learn be transitioned controlled environment field. Complex synthesize accumulate high levels fatty acids modified oils in seeds, can deployed, tested undergo rounds iteration agronomically relevant environments. Engineered are now increasingly being developed used sustainably supply, improved nutrition, feed, biofuels fossil fuel replacements high-value chemical In this review, we provide summary acid synthesis oil assembly highlighting how discovery supports advance towards predictive manipulation produce desirable bio-based Further examples innovation improvement provided, describing technologies (e.g., modification (GM), gene editing (GE), RNAi, alongside GM GE stacking) applied new products denude undesirable traits. Focusing on production long chain polyunsaturated omega-3 describe transition commercial prototype. prospects structured triacylglycerol specified stereospecific positions also discussed, future outlook agronomic uptake biotechnology.
Language: Английский
Citations
0
Analytical Methods, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 1, 2025
Based on a non-targeted metabolomic approach, comprehensive analysis of the metabolic profiles two raspberry cultivars, Selina (TSM) and Tulameen (CSM), was conducted to explore potential for further processing these varieties.
Language: Английский
Citations
0
Scientific Data, Journal Year: 2025, Volume and Issue: 12(1)
Published: Feb. 20, 2025
Seed development, which depends on parent plants genetic background and mother plant environmental conditions, is a major component determining seed composition. quality main agricultural concern, impacting both food non-food applications, while also playing central role in biodiversity conservation environment protection. Climate change, particular the emergence of extremely high temperatures, constitute critical global threat to agriculture. Specialized metabolites (SMs) play crucial roles interactions seeds with their environments. Several SMs are known be protective compounds involved stress responses, thus quality. In this study, we performed untargeted metabolomic (LC-MS/MS) transcriptomic (RNA-Seq) analyses Arabidopsis thaliana harvested at six developmental stages (Globular, Transition, Torpedo, Bent cotyledon, Mature green Dry seed), developed under control warm temperature conditions. Those data provide an original valuable resource that could used identify genes heat responses for study regulation functions during development.
Language: Английский
Citations
0
PLANT PHYSIOLOGY, Journal Year: 2024, Volume and Issue: 197(1)
Published: Nov. 8, 2024
Abstract Jasmonates (JAs) are important phytohormones that regulate plant tolerance to biotic and abiotic stresses, developmental processes. Distinct JAs in different lineages activate a conserved signaling pathway mediates these responses: dinor-12-oxo-phytodienoic acid (dn-OPDA) isomers bryophytes lycophytes, JA-Ile most vascular plants. In many cases, the final responses triggered by depend on accumulation of specialized metabolites. To identify compounds regulated dn-OPDA liverwort Marchantia polymorpha, untargeted metabolomic analyses were carried out response wounding, stress activates pathway. A previously unreported group molecules was identified from analyses: dn-OPDA-amino conjugates (dn-OPDA-aas). Their after wounding herbivory confirmed targeted metabolic profiling all species which we detected dn-iso-OPDA. Mutants GRETCHEN-HAGEN 3A (MpGH3A) failed accumulate dn-OPDA-aa showed constitutive activation OPDA increased resistance herbivory. Our results show dn-iso-OPDA bioactivity is reduced amino conjugation. Therefore, JA conjugation land plants plays dichotomous roles: jasmonic with isoleucine (Ile) produces bioactive tracheophytes, whereas acids deactivates phytohormone lycophytes.
Language: Английский
Citations
3