Multi‐omic analyses unveil contrasting composition and spatial distribution of specialized metabolites in seeds of Camelina sativa and other Brassicaceae

The Plant Journal, Journal Year: 2025, Volume and Issue: 121(3)

Published: Feb. 1, 2025

SUMMARY Seeds of Brassicaceae produce a large diversity beneficial and antinutritional specialized metabolites (SMs) that influence their quality provide resistance to stresses. While SM distribution has been described in leaves root tissues, limited information is available about spatiotemporal accumulation seeds. Camelina sativa (camelina) an oilseed cultivated for human animal nutrition industrial uses. we previously explored plasticity, no expression related proteins genes camelina In this study, used multi‐omic approach, integrating untargeted metabolomics, proteomics, transcriptomics investigate the synthesis, modification, degradation SMs accumulated seed tissues (seed coat, endosperm, embryo) at six developmental two germination stages. Metabolomic results showed distinct patterns pathways, highlighting significant contrasts composition spatial defense‐related glucosinolate (GSL) compounds among camelina, Arabidopsis thaliana , Brassica napus three closely species. Notably, thanks metabolomic proteomic/transcriptomic techniques variation GSL distributions was primarily driven by differences structure (metabolomics data) transport (transcriptomic proteomic mechanisms. Long‐chain C8–C11 methylsulfinylalkyl GSLs were predominantly coat while mid‐ short‐chain C3–C7 embryo. Characterizing dynamics provides valuable insights can guide development crops with optimized toxic metabolites, improving nutritional profiles.

Language: Английский

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Specialized metabolite modifications in Brassicaceae seeds and plants: diversity, functions and related enzymes

Natural Product Reports, Journal Year: 2024, Volume and Issue: 41(5), P. 834 - 859

Published: Jan. 1, 2024

Diversity, functions and related enzymes of Specialized Metabolite (SM) modifications from plants seeds Brassicaceae are summarized in this review. A particular focus is made on SM plasticity modification seeds.

Language: Английский

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Seed longevity and genome damage

Bioscience Reports, Journal Year: 2024, Volume and Issue: 44(2)

Published: Feb. 1, 2024

Seeds are the mode of propagation for most plant species and form basis both agriculture ecosystems. Desiccation tolerant seeds, representative crop species, can survive maturation drying to become metabolically quiescent. The desiccated state prolongs embryo viability provides protection from adverse environmental conditions, including seasonal periods drought freezing often encountered in temperate regions. However, capacity seed germinate declines over time culminates loss viability. relationship between conditions (temperature humidity) rate deterioration (ageing) is well defined, but less known about biochemical genetic factors that determine longevity. This review will highlight recent advances our knowledge provide insight into cellular stresses protective mechanisms promote survival, with a focus on roles DNA repair response mechanisms. Collectively, these pathways function maintain germination potential seeds. Understanding molecular longevity important new targets production crops enhanced resilience changing climates preservation germplasm seedbanks.

Language: Английский

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Proteomic and metabolomic insights into seed germination of Ferula assa-foetida

Journal of Proteomics, Journal Year: 2024, Volume and Issue: 300, P. 105176 - 105176

Published: April 9, 2024

Language: Английский

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Specialized metabolome and transcriptome atlas of developing Arabidopsis thaliana seed under warm temperatures

Scientific Data, Journal Year: 2025, Volume and Issue: 12(1)

Published: Feb. 20, 2025

Seed development, which depends on parent plants genetic background and mother plant environmental conditions, is a major component determining seed composition. quality main agricultural concern, impacting both food non-food applications, while also playing central role in biodiversity conservation environment protection. Climate change, particular the emergence of extremely high temperatures, constitute critical global threat to agriculture. Specialized metabolites (SMs) play crucial roles interactions seeds with their environments. Several SMs are known be protective compounds involved stress responses, thus quality. In this study, we performed untargeted metabolomic (LC-MS/MS) transcriptomic (RNA-Seq) analyses Arabidopsis thaliana harvested at six developmental stages (Globular, Transition, Torpedo, Bent cotyledon, Mature green Dry seed), developed under control warm temperature conditions. Those data provide an original valuable resource that could used identify genes heat responses for study regulation functions during development.

Language: Английский

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Deciphering seed development and germination in the single-cell era

Seed Science Research, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 10

Published: March 3, 2025

Abstract Seeds are complex structures that serve as dispersal units in angiosperms. consist of three specialized tissues with distinct roles and molecular compositions. Hence, the characterization genetic regulators act within individual seed tissues, how their activity changes during development germination, has been a primary focus research. However, our knowledge spatiotemporal modulation seeds, across different cell types, limited by resolution available techniques. In last few years, application single-cell technologies plants have enabled elucidation gene networks involved various developmental processes at cellular level. Some studies applied these to enabling further germination Here, we review current status seeds present workflow for conducting transcriptomics. Additionally, discuss integration multi-omics, aiming demonstrate potential enhancing comprehension regulations governing germination.

Language: Английский

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Reactive oxygen and nitrogen species (RONS) signalling in seed dormancy release, perception of environmental cues, and heat stress response

Plant Growth Regulation, Journal Year: 2023, Volume and Issue: 103(1), P. 9 - 32

Published: Dec. 15, 2023

Abstract Seed germination is a crucial plant-life process whose success depends largely on the seed's ability to germinate under favourable environmental conditions. Through molecular signalling, seed able perceive information, assimilate it, and transmit signals that determine its destiny. Reactive Oxygen Nitrogen Species (RONS) function as signalling molecules influence multiple phases of plant development. In germination, their presence generally promotes completion, though not same extent in all species environments. As molecules, they participate sensing light temperature fluctuations cues, but also play role inhibiting when temperatures exceed optimal range, preventing seedling exposure heat. Depending conditions, RONS set up crosstalk with major phytohormones involved ABA, GA, even auxin, regulating biosynthesis signalling. Here, we show relevant studies how exert control levels, such through protein oxidation, epigenetic control, promotion phytohormone key-metabolism genes expression, post-translational modifications, redox interactions DOG1. This review summarises current understanding seed, from maturation transduction Special consideration given RONS-mediated response stimuli, or fluctuations, conditions inhibit high temperatures.

Language: Английский

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Proanthocyanidin biosynthesis and postharvest seed coat darkening in pinto bean

Phytochemistry Reviews, Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 12, 2023

Abstract Proanthocyanidins (PAs) are polyphenolic compounds present widely in the plant kingdom. These specialized metabolites derived from phenylpropanoid pathway and known for producing brown pigments different organs. PAs accumulate seed coat tissues of flowering plants play a determinant role germination viability, protect seeds biotic abiotic stresses, thus ensure long-term storage potential seeds. In addition, rich source antioxidants health both human livestock. Many commercially relevant dry beans ( Phaseolus vulgaris ) contain high levels PAs, which when oxidized cause to overdarken, phenomenon as postharvest darkening. darker give impression oldness, consumers tend avoid buying them which, turn, affects their market value. Pinto beans, one leading classes affected most by Therefore, exploring regulation PA biosynthesis accumulation bean will help develop strategy manage darkening effect pintos. this review, we discuss its regulation, connecting it color genetics better understanding mechanism

Language: Английский

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Understanding the role of storage reserve mobilization during seed germination and initial seedling growth in species of the genus Carapa

Botany, Journal Year: 2024, Volume and Issue: 102(6), P. 268 - 281

Published: March 25, 2024

A physiological and metabolic database for various species of Amazonian plants is crucial to the selection sustainable utilization their diversity. We hypothesized that understanding mobilization Carapa seed reserves water uptake during germination could provide insights into behavior propagation differentiate same genus. Given this, mechanisms primary reserves, uptake, enzymatic activities in seeds two ( guianensis vasquezii) were investigated. The lipid content was high both species, with a value 63.7 ± 4.7% C. 55 1.5% vasquezii. showed higher values other evaluated reserves. protein profile highlights band between 15 25 kDa guianensis. As investigated (lipase, amylase, protease, amd acid phosphatase), greater activity most them, except phosphatase. Overall, different strategies dynamics regarding use Our findings indicate distinct pathways are employed initial seedling growth stages species. This trait can potentially guide conservation these

Language: Английский

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G protein regulation in rice seed biology

Seed Biology, Journal Year: 2024, Volume and Issue: 3(1), P. 0 - 0

Published: Jan. 1, 2024

Seeds are not only the organ of producing progenies but also vector grain quality and yield in cereal crops. Researches focusing on seed biology to improve crops is important for ensuring food security addressing hunger issues worldwide. The heterotrimeric G protein consists Gα, Gβ Gγ subunits, which acts as a conserved signaling switch transmit extracellular signals downstream effectors, thereby regulating various biological functions. proteins rice control numerous agronomic traits, biotic abiotic stress responses, with significant application potential, has drawn increasing attention by researchers breeders. Here, we summarize recent progresses development, including size, number, yield, resistance, focused molecular cellular regulation pathway biology, offered some understandings potential applications. Investigating can address critical development offers novel insights into breeding. Although it clear pathways mammals, upstream signals, receptors direct effectors plants still unknown many progresses. We thus propose 5 key scientific questions underlying call an international coordinated effort studying solving these problems form project named 5QGPS (short plants).

Language: Английский

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