All families of transposable elements were active in the recent wheat genome evolution and polyploidy had no impact on their activity DOI Creative Commons

Nathan Papon,

Pauline Lasserre‐Zuber,

Hélène Rimbert

et al.

The Plant Genome, Journal Year: 2023, Volume and Issue: 16(3)

Published: May 26, 2023

Bread wheat (Triticum aestivum L.) is a major crop and its genome one of the largest ever assembled at reference-quality level. It 15 Gb, hexaploid, with 85% transposable elements (TEs). Wheat genetic diversity was mainly focused on genes little known about extent genomic variability affecting TEs, transposition rate, impact polyploidy. Multiple chromosome-scale assemblies are now available for bread tetraploid diploid wild relatives. In this study, we computed base pair-resolved, gene-anchored, whole alignments A, B, D lineages different ploidy levels in order to estimate that affects TE space. We used genomes 13 T. cultivars (6x = AABBDD) single Triticum durum (4x AABB), dicoccoides urartu (2x AA), Aegilops tauschii DD). show 5%-34% fraction variable, depending species divergence. Between 400 13,000 novel insertions per subgenome were detected. found lineage-specific nearly all families di-, tetra-, hexaploids. No burst observed polyploidization did not trigger any boost transposition. This study challenges prevailing idea dynamics more agreement an equilibrium model evolution.

Language: Английский

Evolution and origin of bread wheat DOI Creative Commons
Avraham A. Levy,

Moshe Feldman

The Plant Cell, Journal Year: 2022, Volume and Issue: 34(7), P. 2549 - 2567

Published: May 1, 2022

Bread wheat (Triticum aestivum, genome BBAADD) is a young hexaploid species formed only 8,500-9,000 years ago through hybridization between domesticated free-threshing tetraploid progenitor, BBAA, and Aegilops tauschii, the diploid donor of D subgenome. Very soon after its formation, it spread globally from cradle in fertile crescent into new habitats climates, to become staple food humanity. This extraordinary global expansion was probably enabled by allopolyploidy that accelerated genetic novelty acquisition traits, intergenomic interactions, buffering mutations, attractiveness bread wheat's large, tasty, nutritious grain with high baking quality. New sequences suggest elusive B subgenome distinct (unknown or extinct) rather than mosaic genome. We discuss origin progenitors conflicting archaeological evidence on where which progenitor. Wheat experienced many environmental changes throughout evolution, therefore, while might adapt current climatic changes, efforts are needed better use conserve vast gene pool biodiversity our security depends.

Language: Английский

Citations

142
Wheat genomic study for genetic improvement of traits in China DOI
Jun Xiao, Liu B, Yingyin Yao

et al.

Science China Life Sciences, Journal Year: 2022, Volume and Issue: 65(9), P. 1718 - 1775

Published: Aug. 24, 2022

Language: Английский

Citations

120
Aegilops sharonensis genome-assisted identification of stem rust resistance gene Sr62 DOI Creative Commons
Guotai Yu, Oadi Matny, Nicolas Champouret

et al.

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: March 25, 2022

Abstract The wild relatives and progenitors of wheat have been widely used as sources disease resistance ( R ) genes. Molecular identification characterization these genes facilitates their manipulation tracking in breeding programmes. Here, we develop a reference-quality genome assembly the diploid relative Aegilops sharonensis use positional mapping, mutagenesis, RNA-Seq transgenesis to identify stem rust gene Sr62 , which has also transferred common wheat. This encodes tandem kinase, homologues exist across multiple taxa plant kingdom. Stable transgenic lines show high levels against diverse isolates pathogen, highlighting utility for deployment part polygenic stack maximize durability resistance.

Language: Английский

Citations

90
Multi-Omics Pipeline and Omics-Integration Approach to Decipher Plant’s Abiotic Stress Tolerance Responses DOI Open Access
Rajib Roychowdhury,

Soumya Prakash Das,

Amber Gupta

et al.

Genes, Journal Year: 2023, Volume and Issue: 14(6), P. 1281 - 1281

Published: June 16, 2023

The present day's ongoing global warming and climate change adversely affect plants through imposing environmental (abiotic) stresses disease pressure. major abiotic factors such as drought, heat, cold, salinity, etc., hamper a plant's innate growth development, resulting in reduced yield quality, with the possibility of undesired traits. In 21st century, advent high-throughput sequencing tools, state-of-the-art biotechnological techniques bioinformatic analyzing pipelines led to easy characterization plant traits for stress response tolerance mechanisms by applying 'omics' toolbox. Panomics pipeline including genomics, transcriptomics, proteomics, metabolomics, epigenomics, proteogenomics, interactomics, ionomics, phenomics, have become very handy nowadays. This is important produce climate-smart future crops proper understanding molecular responses genes, transcripts, proteins, epigenome, cellular metabolic circuits resultant phenotype. Instead mono-omics, two or more (hence 'multi-omics') integrated-omics approaches can decipher well. Multi-omics-characterized be used potent genetic resources incorporate into breeding program. For practical utility crop improvement, multi-omics particular combined genome-assisted (GAB) being pyramided improved yield, food quality associated agronomic open new era omics-assisted breeding. Thus, together are able processes, biomarkers, targets engineering, regulatory networks precision agriculture solutions crop's variable ensure security under changing circumstances.

Language: Английский

Citations

88
Wheat powdery mildew resistance gene Pm13 encodes a mixed lineage kinase domain-like protein DOI Creative Commons
Huanhuan Li,

Wenqiang Men,

Chao Ma

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: March 19, 2024

Abstract Wheat powdery mildew is one of the most destructive diseases threatening global wheat production. The wild relatives constitute rich sources diversity for resistance. Here, we report map-based cloning resistance gene Pm13 from species Aegilops longissima . encodes a mixed lineage kinase domain-like (MLKL) protein that contains an N-terminal-domain MLKL (MLKL_NTD) domain in its N-terminus and C-terminal serine/threonine (STK) domain. function validated by mutagenesis, silencing, transgenic assay, allelic association analyses. development introgression lines with significantly reduced chromosome segments Ae. encompassing enables widespread deployment this into cultivars. may provide valuable insights molecular mechanisms underlying -mediated highlight important roles fusion proteins (KFPs) immunity.

Language: Английский

Citations

21
Variation in TaSPL6-D confers salinity tolerance in bread wheat by activating TaHKT1;5-D while preserving yield-related traits DOI
Meng Wang, Jie Cheng, Jianhui Wu

et al.

Nature Genetics, Journal Year: 2024, Volume and Issue: 56(6), P. 1257 - 1269

Published: May 27, 2024

Language: Английский

Citations

18
Introducing Beneficial Alleles from Plant Genetic Resources into the Wheat Germplasm DOI Creative Commons
Shivali Sharma, Albert W. Schulthess, Filippo M. Bassi

et al.

Biology, Journal Year: 2021, Volume and Issue: 10(10), P. 982 - 982

Published: Sept. 29, 2021

Wheat (

Language: Английский

Citations

78
Detecting major introgressions in wheat and their putative origins using coverage analysis DOI Creative Commons
Jens Keilwagen, Heike Lehnert,

Thomas Berner

et al.

Scientific Reports, Journal Year: 2022, Volume and Issue: 12(1)

Published: Feb. 3, 2022

Introgressions from crop wild relatives (CWRs) have been used to introduce beneficial traits into cultivated plants. traditionally detected using cytological methods. Recently, single nucleotide polymorphism (SNP)-based methods proposed detect introgressions in crosses for which both parents are known. However, unknown material, no method was available and predict the putative donor species. Here, we present a We demonstrate utility of this 10 publicly wheat genome sequences identify nine major introgressions. show that can distinguish different at same locus. trace early cultivars natural were utilised breeding history still influence elite lines today. Finally, provide evidence these harbour resistance genes.

Language: Английский

Citations

43
Orthologous genes Pm12 and Pm21 from two wild relatives of wheat show evolutionary conservation but divergent powdery mildew resistance DOI Creative Commons
Shanying Zhu, Cheng Liu,

Shuangjun Gong

et al.

Plant Communications, Journal Year: 2022, Volume and Issue: 4(2), P. 100472 - 100472

Published: Nov. 9, 2022

Wheat powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a devastating disease that threatens wheat production worldwide. Pm12, which originated from Aegilops speltoides, wild relative of wheat, confers strong resistance to mildew and therefore has potential use in breeding. Using susceptible mutants induced gamma irradiation, we physically mapped isolated Pm12 showed it be orthologous Pm21 Dasypyrum villosum, also wheat. The function was validated via ethyl methanesulfonate mutagenesis, virus-induced gene silencing, stable genetic transformation. Evolutionary analysis indicates the Pm12/Pm21 loci species are relatively conserved but dynamic. Here, demonstrated two genes, Pm21, possess differential against same set Bgt isolates. Overexpression coiled-coil domains both PM12 PM21 induces cell death Nicotiana benthamiana leaves. However, their full-length forms display different death-inducing activities distinct intramolecular interactions. Cloning will facilitate its application breeding programs. This study gives new insight into show race specificities interaction patterns.

Language: Английский

Citations

43
Cloning of the wheat leaf rust resistance gene Lr47 introgressed from Aegilops speltoides DOI Creative Commons
Hongna Li, Lei Hua, Shuqing Zhao

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Sept. 28, 2023

Leaf rust, caused by Puccinia triticina Eriksson (Pt), is one of the most severe foliar diseases wheat. Breeding for leaf rust resistance a practical and sustainable method to control this devastating disease. Here, we report identification Lr47, broadly effective gene introgressed into wheat from Aegilops speltoides. Lr47 encodes coiled-coil nucleotide-binding leucine-rich repeat protein that both necessary sufficient confer Pt resistance, as demonstrated loss-of-function mutations transgenic complementation. introgression lines with no or reduced linkage drag are generated using Pairing homoeologous1 mutation, diagnostic molecular marker developed. The domain unable induce cell death, nor does it have self-protein interaction. cloning expands number genes can be incorporated multigene cassettes

Language: Английский

Citations

32