Tree Physiology,
Journal Year:
2024,
Volume and Issue:
unknown
Published: May 30, 2024
Abstract
miR156
play
important
roles
in
regulation
of
plant
growth
and
development,
secondary
metabolite
synthesis
other
biological
processes
by
targeting
the
SQUAMOSA
promoter
binding
protein-like
(SPL)
family.
Our
previous
sequencing
data
analysis
suggested
that
Csn-miR156d
may
regulate
flowering
anthocyanin
accumulation
cleavage
degradation
expression
SPL
tea
plant,
but
it
remains
to
be
elucidated.
In
this
study,
5′RLM-RACE
experiment,
tobacco
transient
transformation,
qRT-PCR
antisense
oligonucleotide
(asODN)
were
used
verify
CsSPL1
is
target
gene
Csn-miR156d.
Stable
transformation
Arabidopsis
revealed
could
delay
negatively
regulating
transcript
levels
FT,
AP1,
FUL
SOC1,
while
overexpression
showed
an
opposite
effect.
Additionally,
enhance
transcription
biosynthesis-related
structural
genes
DFR,
ANS,
F3H,
UGT78D2
LDOX,
as
well
regulatory
PAP1,
MYB113,
GL3,
MYB11
MYB12,
leading
accumulation.
Moreover,
asODN
experiment
increase
content
plant.
These
results
suggest
regulates
suppressing
CsSPL1.
study
provides
new
insights
into
development
lays
a
theoretical
foundation
for
further
research
on
molecular
mechanism
miRNAs
metabolism.
PLANT PHYSIOLOGY,
Journal Year:
2023,
Volume and Issue:
193(1), P. 840 - 854
Published: June 16, 2023
As
the
harvest
season
of
most
fruit
is
concentrated,
maturation
manipulation
essential
for
fresh
industry
to
prolong
sales
time.
Gibberellin
(GA),
an
important
phytohormone
necessary
plant
growth
and
development,
has
also
shown
a
substantial
regulatory
effect
on
maturation;
however,
its
mechanisms
remain
inconclusive.
In
this
research,
preharvest
GA3
treatment
effectively
delayed
in
several
persimmon
(Diospyros
kaki)
cultivars.
Among
proteins
encoded
by
differentially
expressed
genes,
2
transcriptional
activators
(NAC
TRANSCRIPTION
FACTOR
DkNAC24
ETHYLENE
RESPONSIVE
DkERF38)
repressor
(MYB-LIKE
DkMYB22)
were
direct
regulators
GERANYLGERANYL
DIPHOSPHATE
SYNTHASE
DkGGPS1,
LYSINE
HISTIDINE
TRANSPORTER
DkLHT1,
FRUCTOSE-BISPHOSPHATE
ALDOLASE
DkFBA1,
respectively,
resulting
inhibition
carotenoid
synthesis,
outward
transport
ethylene
precursor,
consumption
fructose
glucose.
Thus,
present
study
not
only
provides
practical
method
period
various
cultivars
but
insights
into
GA
multiple
aspects
quality
formation
at
regulation
level.
International Journal of Molecular Sciences,
Journal Year:
2023,
Volume and Issue:
24(15), P. 12185 - 12185
Published: July 29, 2023
MYB
and
BBX
transcription
factors
play
important
roles
in
flavonoid
biosynthesis.
Here,
we
obtained
transgenic
woodland
strawberry
with
stable
overexpression
of
FaMYB5,
demonstrating
that
FaMYB5
can
increase
anthocyanin
proanthocyanidin
content
roots,
stems
leaves
strawberry.
In
addition,
bimolecular
fluorescence
complementation
assays
yeast
two-hybridization
demonstrated
the
N-terminal
(1-99aa)
FaBBX24
interacts
FaMYB5.
Transient
co-expression
cultivated
‘Xiaobai’
showed
strongly
promoted
expression
F3′H,
4CL-2,
TT12,
AHA10
ANR
then
increased
fruits.
We
also
determined
is
a
positive
regulator
biosynthesis
The
results
reveal
novel
mechanism
by
which
FaMYB5–FaBBX24
module
collaboratively
regulates
fruit.
PLANT PHYSIOLOGY,
Journal Year:
2023,
Volume and Issue:
194(3), P. 1577 - 1592
Published: Nov. 25, 2023
Abstract
The
improvement
of
fruit
quality,
in
particular
sugar
content,
has
been
a
major
goal
plant
breeding
programmes
for
many
years.
Here,
2
varieties
the
Ussurian
pear
(Pyrus
ussuriensis),
Nanguo,
and
its
high-sucrose
accumulation
bud
sport,
Nanhong,
were
used
to
study
molecular
mechanisms
regulating
sucrose
transport
fruits.
Comparative
transcriptome
analysis
showed
that
Nanhong
fruit,
an
MYB
transcription
factor,
PuMYB12,
transporter
protein,
PuSUT4-like,
expressed
at
higher
levels,
while
paclobutrazol
resistance
PuPRE6,
histone
deacetylase
(HDAC),
PuHDAC9-like,
lower
levels
Nanguo
fruit.
PuSUT4-like
silencing
overexpression
experiments
is
essential
transportation.
PuPRE6
PuMYB12
act
as
antagonistic
complexes
regulate
accumulation.
deacetylation
promoters
than
Y1H
assays
HDAC
PuHDAC9-like
bound
directly
PuSUT4-like.
Our
results
uncovered
regulation
epigenetic
underlying
pears.
Tree Physiology,
Journal Year:
2024,
Volume and Issue:
unknown
Published: May 30, 2024
Abstract
miR156
play
important
roles
in
regulation
of
plant
growth
and
development,
secondary
metabolite
synthesis
other
biological
processes
by
targeting
the
SQUAMOSA
promoter
binding
protein-like
(SPL)
family.
Our
previous
sequencing
data
analysis
suggested
that
Csn-miR156d
may
regulate
flowering
anthocyanin
accumulation
cleavage
degradation
expression
SPL
tea
plant,
but
it
remains
to
be
elucidated.
In
this
study,
5′RLM-RACE
experiment,
tobacco
transient
transformation,
qRT-PCR
antisense
oligonucleotide
(asODN)
were
used
verify
CsSPL1
is
target
gene
Csn-miR156d.
Stable
transformation
Arabidopsis
revealed
could
delay
negatively
regulating
transcript
levels
FT,
AP1,
FUL
SOC1,
while
overexpression
showed
an
opposite
effect.
Additionally,
enhance
transcription
biosynthesis-related
structural
genes
DFR,
ANS,
F3H,
UGT78D2
LDOX,
as
well
regulatory
PAP1,
MYB113,
GL3,
MYB11
MYB12,
leading
accumulation.
Moreover,
asODN
experiment
increase
content
plant.
These
results
suggest
regulates
suppressing
CsSPL1.
study
provides
new
insights
into
development
lays
a
theoretical
foundation
for
further
research
on
molecular
mechanism
miRNAs
metabolism.
