Laser & Optoelectronics Progress, Journal Year: 2023, Volume and Issue: 60(8), P. 0811016 - 0811016
Published: Jan. 1, 2023
Photonics Research, Journal Year: 2024, Volume and Issue: 12(7), P. 1494 - 1494
Published: May 9, 2024
The transport-of-intensity equation (TIE) enables quantitative phase imaging (QPI) under partially coherent illumination by measuring the through-focus intensities combined with a linearized inverse reconstruction algorithm. However, overcoming its sensitivity to settings remains challenging problem because of difficulty in tuning optical parameters system accurately and instability long-time measurements. To address these limitations, we propose experimentally validate solution called neural-field-assisted microscopy (NFTPM) introducing tunable defocus parameter into neural field. Without weak object approximation, NFTPM incorporates physical prior image formation constrain field learns continuous representation without need for training. Simulation experimental results HeLa cells demonstrate that can achieve accurate, QPI unknown distances, providing new possibilities extending applications live cell biology.
Language: Английский
Citations
19
Applied Physics B, Journal Year: 2024, Volume and Issue: 130(9)
Published: Aug. 29, 2024
Computational methods have been established as cornerstones in optical imaging and holography recent years. Every year, the dependence of on computational is increasing significantly to extent that components are being completely efficiently replaced with at low cost. This roadmap reviews current scenario four major areas namely incoherent digital holography, quantitative phase imaging, through scattering layers, super-resolution imaging. In addition registering perspectives modern-day architects above research areas, also reports some latest studies topic. codes pseudocodes presented for a plug-and-play fashion readers not only read understand but practice algorithms their data. We believe this will be valuable tool analyzing trends predict prepare future holography.
Language: Английский
Citations
13
Laser & Photonics Review, Journal Year: 2024, Volume and Issue: 18(4)
Published: Jan. 7, 2024
Abstract High spatial resolution imaging over the entire volume for intravital of biological specimens has long been vital. However, depth‐of‐field (DOF) and are intrinsically interdependent. Here, a new extended DOF (EDOF) microscopy technique based on partially coherent annular illumination is proposed, termed AI‐EDOF, by combining an electrically tunable lens with conventional microscope modified illumination, motion‐free, high real‐time all‐in‐focus thick volumes. EDOF obtained up to near incoherent diffraction limit (388 nm, 20/ 0.8NA) temporal 30 fps. Moreover, it demonstrated that coupled phase absorption components complex refractive index in optical tomography can be solved focus scanning. The Richardson‐Lucy deconvolution total variation regularization adopted deblurring suppressing noise under low light efficiency high‐speed exposure. To demonstrate capabilities proposed method, experiments conducted using fixed transgenic zebrafish larvae, Drosophila larvae dynamic Caenorhabditis elegans both transmissive fluorescent modalities, revealing approach prospective broad applications such as pharmacokinetics tumor immunology.
Language: Английский
Citations
12
Optics Letters, Journal Year: 2025, Volume and Issue: 50(3), P. 908 - 908
Published: Jan. 2, 2025
Polarization sensitivity is a fundamental phenomenon observed in nature, and its application vital for advancing scientific discoveries. Here, we present microsphere-assisted polarized light microscopy method that enables high-quality, label-free super-resolution imaging of polarization-sensitive materials. We investigated the performance this using various samples, including colloidal crystals grating structures. Both simulations experiments were performed to explore underlying physical mechanisms driving enhancement quality. The results work have potential values fields imaging.
Language: Английский
Citations
1
Laser & Photonics Review, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 28, 2025
Abstract Intensity diffraction tomography (IDT) and fluorescence dual‐modality imaging facilitate a comprehensive analysis of biological components their interactions. However, long‐term is typically compromised by environmental thermal fluctuations mechanical disturbances from the microscope, leading to time‐varying aberrations focus drift that degrades performance accuracy colocalization. To counter these issues, an adaptive optics‐assisted 3D method (AO‐FIDT) developed. The utilizes innovative iterative ptychographic approach, paired with annular matched illumination conditions, precisely reconstruct IDT results characterize in real‐time. Furthermore, feedback on real‐time point spread function system provided synchronously correct results. efficacy precision AO‐FIDT are confirmed through long‐term, high‐resolution HeLa cells. scrutinizing morphological characteristics subcellular organelles live COS‐7 cells, including progressive sphericity mitochondria under phototoxicity, monitoring continuous changes mitochondrial dynamics throughout cell division process, broad applicability analyzing organelle structure demonstrated.
Language: Английский
Citations
1
APL Photonics, Journal Year: 2024, Volume and Issue: 9(1)
Published: Jan. 1, 2024
We report a wavelength-scanning-based lens-free on-chip microscope using color CMOS sensor and matching modified phase retrieval algorithm for pixel super-resolution. Compared to traditional monochrome industrial cameras, sensors favored by the consumer electronics industry have smaller sizes, higher performance, lower costs. However, filtering array (CFA) introduces inherent modulation holograms acquired under quasi-monochromatic illumination, which complicates data processing in microscopy. Without physically removing CFA positioned on chip, we demonstrate quantitative imaging (QPI) with lateral half-width resolution of 615 nm over wide field-of-view 51.88 mm2 exploiting green-channel from Bayer-masked holograms. The resulting spatial bandwidth product is 137.2 megapixels, 10 times that conventional optical microscope. rationale only information each sampling point not lost during propagation but rather distributed all pixels image. Therefore, missing other channels can be recovered sufficient differences among raw images captured at different wavelengths. scheme sensors, this method requires acquisition several more guarantee convergence algorithm. Experimental results show achieve high-quality QPI thus demonstrating applicability cost-effective field holographic
Language: Английский
Citations
7
Journal of Biomedical Optics, Journal Year: 2024, Volume and Issue: 29(S2)
Published: March 26, 2024
SignificanceQuantitative phase imaging (QPI) offers a label-free approach to non-invasively characterize cellular processes by exploiting their refractive index based intrinsic contrast. QPI captures this contrast translating associated shifts into intensity-based quantifiable data with nanoscale sensitivity. It holds significant potential for advancing precision cancer medicine providing quantitative characterization of the biophysical properties cells and tissue in natural states.AimThis perspective aims discuss increase our understanding development its response therapeutics. also explores new developments methods towards personalized therapy early detection.ApproachWe begin detailing technical advancements QPI, examining implementations across transmission reflection geometries retrieval methods, both interferometric non-interferometric. The focus then QPI's applications research, including dynamic cell mass drug assessment, risk stratification, in-vivo imaging.ResultsQPI has emerged as crucial tool medicine, offering insights tumor biology treatment efficacy. Its sensitivity detecting changes promise enhancing diagnostics, prognostication. future is envisioned integration artificial intelligence, morpho-dynamics, spatial biology, broadening impact research.ConclusionsQPI presents redefining diagnosis, monitoring, treatment. Future directions include harnessing high-throughput imaging, 3D realistic models, combining intelligence multi-omics extend capabilities. As result, stands at forefront research clinical application care.
Language: Английский
Citations
7
ACS Photonics, Journal Year: 2023, Volume and Issue: 10(8), P. 2461 - 2466
Published: May 22, 2023
Optical diffraction tomography (ODT) is a powerful tool for the study of unlabeled biological cells thanks to its unique capability measuring three-dimensional (3D) refractive index (RI) distribution samples quantitatively and noninvasively. In conventional transmission ODT, however, certain spatial frequency components along optical axis cannot be measured due limited angular coverage incident beam, resulting in poor axial resolution several times worse than lateral one. this Letter we propose new type ODT method, termed opposite illumination Fourier ptychographic (OI-FPDT), which produces almost isotropic by combining transmissive angle-scanning reflective wavelength-scanning. Without resorting interferometric detection, OI-FPDT requires an intensity-only measurement, forward backward scattered intensity images are synthesized space recover 3D RI based on iterative reconstruction algorithm. To best our knowledge, first time that near-isotropic (∼ 274 nm) result obtained non-interferometric sample motion-free manner. Results simulated cell phantom, tailor-made fiberglass, onion epidermal confirm validity proposed method.
Language: Английский
Citations
16
Deleted Journal, Journal Year: 2024, Volume and Issue: 1(1)
Published: June 28, 2024
Abstract Lens-free on-chip microscopy (LFOCM) has been widely utilized in digital pathology, drug screening, point-of-care testing (POCT), and quantitative phase imaging (QPI) due to its high throughput capability compactness. Initially, coherent laser sources were used LFOCM generate interference fringes reconstruct the intensity information of an object. The use partially light-emitting diodes (LEDs) offers a more portable cost-effective alternative conventional illumination sources. However, coherence-gating effect from relatively low degree coherence may cause blur high-frequency holograms, leading inaccurate object recovery. Thus, we present pixel-super-resolved lens-free (PSR-LFQPM) with illumination, which not only compensates for impact without increasing volume system but also suppresses theoretical Nyquist-Shannon sampling resolution limit imposed by sensor pixel size (0.9 μm). Based on model, integrate spatial transfer function (SCTF) obtained pre-calibrated LED source distribution during iteration process obtain accurate high-resolution Applying PSR-LFQPM image living HeLa cells vitro, achieve real-time dynamic high-throughput QPI performance (half-pitch 780 nm 1.41-fold improvement compared results considering coherence) across wide FOV (19.53 mm 2 ). proposed method provides compact, low-cost, biomedical POCT applications.
Language: Английский
Citations
4
Applied Physics Letters, Journal Year: 2025, Volume and Issue: 126(1)
Published: Jan. 2, 2025
Coherent diffractive imaging (CDI), especially the ptychography, has been widely used in quantitative phase imaging. However, traditional schemes, a highly coherent light source is required for better-posed inverse problem. Considering that high coherence cannot be guaranteed all cases and to avoid multi-mode of low-coherence sources complicating iterative modeling imaging, we proposed an overlap-scanning self-referencing As non-iterative partially method, was combined with procedure ptychography. It does not require prior knowledge coherence, only problem needed solved final stitching. demonstrated able recover complex amplitude objects illuminated by various sources. This study presents significant potential applications wide-field view under incoherent illuminations.
Language: Английский
Citations
0