Point spread function engineering based on wavefront phase modulation DOI
Wei-Qiang Zhang,

Xiaojin Cui,

Junbai Yue

et al.

Published: Nov. 27, 2023

Point spread function (PSF) is the response of an optical system to a point source. The ideal PSF traditional optics Airy pattern corresponding images without aberration. However, shape considered be flexible in computational imaging territory, which can artificially manipulated, and target image restored by reconstruction algorithm. It called engineering improve quality system. method break through limitations even endow systems with new capabilities. In this paper, we will discuss based on wavefront phase modulation, more commonly used for its advantage high throughput. Its application, depth field extension in-situ plankton discussed end-to-end joint optimization, demonstrating defocus invariant quality.

Language: Английский

E2E-BPF microscope: extended depth-of-field microscopy using learning-based implementation of binary phase filter and image deconvolution DOI Creative Commons

Baekcheon Seong,

Woovin Kim,

Younghun Kim

et al.

Light Science & Applications, Journal Year: 2023, Volume and Issue: 12(1)

Published: Nov. 13, 2023

Several image-based biomedical diagnoses require high-resolution imaging capabilities at large spatial scales. However, conventional microscopes exhibit an inherent trade-off between depth-of-field (DoF) and resolution, thus objects to be refocused each lateral location, which is time consuming. Here, we present a computational platform, termed E2E-BPF microscope, enables large-area, of large-scale without serial refocusing. This method involves physics-incorporated, deep-learned design binary phase filter (BPF) jointly optimized deconvolution neural network, altogether produces high-resolution, high-contrast images over extended depth ranges. We demonstrate the through numerical simulations experiments with fluorescently labeled beads, cells tissue section, capability 15.5-fold larger DoF than microscope. Our provides highly effective scalable strategy for DoF-extended optical system, expected find numerous applications in rapid diagnosis, vision, metrology.

Language: Английский

Citations

16
A miniaturized mesoscope for the large-scale single-neuron-resolved imaging of neuronal activity in freely behaving mice DOI
Yuanlong Zhang,

Lekang Yuan,

Qiyu Zhu

et al.

Nature Biomedical Engineering, Journal Year: 2024, Volume and Issue: 8(6), P. 754 - 774

Published: June 20, 2024

Language: Английский

Citations

3
Robust single-shot 3D fluorescence imaging in scattering media with a simulator-trained neural network DOI Creative Commons
Jeffrey Alido, J. E. Greene, Yujia Xue

et al.

Optics Express, Journal Year: 2024, Volume and Issue: 32(4), P. 6241 - 6241

Published: Jan. 17, 2024

Imaging through scattering is a pervasive and difficult problem in many biological applications. The high background the exponentially attenuated target signals due to fundamentally limits imaging depth of fluorescence microscopy. Light-field systems are favorable for high-speed volumetric imaging, but 2D-to-3D reconstruction ill-posed, exacerbates condition inverse problem. Here, we develop simulator that models low-contrast buried heterogeneous strong background. We then train deep neural network solely on synthetic data descatter reconstruct 3D volume from single-shot light-field measurement with low signal-to-background ratio (SBR). apply this our previously developed computational miniature mesoscope demonstrate robustness learning algorithm phantoms different conditions. can robustly emitters 2D SBR as 1.05 length. analyze fundamental tradeoffs based design factors out-of-distribution affect model’s generalizability real experimental data. Broadly, believe simulator-based approach be applied wide range techniques where paired training lacking.

Language: Английский

Citations

2
High-resolution multi-z confocal microscopy with a diffractive optical element DOI Creative Commons
Bingying Zhao, Minoru Koyama, Jérôme Mertz

et al.

Biomedical Optics Express, Journal Year: 2023, Volume and Issue: 14(6), P. 3057 - 3057

Published: May 22, 2023

There has been recent interest in the development of fluorescence microscopes that provide high-speed volumetric imaging for life-science applications. For example, multi-z confocal microscopy enables simultaneous optically-sectioned at multiple depths over relatively large fields view. However, to date, hampered by limited spatial resolution owing its initial design. Here we present a variant recovers full conventional microscope while retaining simplicity and ease use our By introducing diffractive optical element illumination path microscope, engineer excitation beam into tightly focused spots are conjugated axially distributed pinholes. We discuss performance this terms detectability demonstrate versatility performing in-vivo beating cardiomyocytes engineered heart tissues neuronal activity c. elegans zebrafish brains.

Language: Английский

Citations

4
Quantitative real-time phase microscopy for extended depth-of-field imaging based on the 3D single-shot differential phase contrast (ssDPC) imaging method DOI Creative Commons
Jingfan Wang, Xing Zhao, Yan Wang

et al.

Optics Express, Journal Year: 2023, Volume and Issue: 32(2), P. 2081 - 2081

Published: Dec. 21, 2023

Optical diffraction tomography (ODT) is a promising label-free imaging method capable of quantitatively measuring the three-dimensional (3D) refractive index distribution transparent samples. In recent years, partially coherent ODT (PC-ODT) has attracted increasing attention due to its system simplicity and absence laser speckle noise. Quantitative phase (QPI) technologies represented by Fourier ptychographic microscopy (FPM), differential contrast (DPC) intensity (IDT) need collect several or hundreds images, which usually introduce motion artifacts when shooting fast-moving targets, leading decrease in image quality. Hence, quantitative real-time (qRPM) for extended depth field (DOF) based on 3D single-shot (ssDPC) proposed this research study. qRPM incorporates microlens array (MLA) simultaneously spatial information angular information. subsequent optical processing, deconvolution used obtain stacks under different illumination angles raw light image. Importing obtained stack into DPC model able finally distribution. The captured four-dimensional enables reconstruction single snapshot extending DOF qRPM. capability experimental verified samples, achieve single-exposure with an 160 µm nearly 30 times higher than traditional microscope system.

Language: Английский

Citations

2
V-shaped PSF for 3D imaging over an extended depth of field in wide-field microscopy DOI
Yunyang Li, Zixiao Zhang, Feng Tian

et al.

Optics Letters, Journal Year: 2024, Volume and Issue: 50(2), P. 383 - 383

Published: Dec. 2, 2024

Single-shot 3D optical microscopy that can capture high-resolution information over a large volume has broad applications in biology. Existing imaging methods using point-spread-function (PSF) engineering often have limited depth of field (DOF) or require custom and complex design phase masks. We propose new, to the best our knowledge, PSF approach is easy implement offers DOF. The appears be axially V-shaped, engineered by replacing conventional tube lens with pair axicon lenses behind objective wide-field microscope. reconstructed from single-shot image deep neural network. Simulations 10× magnification microscope show V-shaped excellent resolution (<2.5 µm lateral ∼15 axial) ∼350 DOF at 550 nm wavelength. Compared other popular PSFs designed for imaging, simple deploy provides high reconstruction quality an extended

Language: Английский

Citations

0
All-in-focus large-FOV GRIN lens imaging by multi-focus image fusion DOI Creative Commons
Dafei Xiao,

Zhige Lin,

Yoav Shechtman

et al.

Optics Continuum, Journal Year: 2023, Volume and Issue: 2(11), P. 2290 - 2290

Published: Sept. 29, 2023

Gradient refractive index (GRIN) lenses are useful for miniaturized and in-vivo imaging. However, the intrinsic field-dependent aberrations of these can deteriorate imaging resolution limit effective field view. To address aberrations, adaptive optics (AO) has been applied which inevitably requires incorporation additional hardware. Here we focus on curvature aberration propose a computational correction scheme fuses z-stack images into single in-focus image over entire view (FOV), with no AO required. We validate our method by all-in-focus wide-field printed letter sample fluorescently labeled mouse brain slices. The also provide what believe to be new valuable option enhancement in scanning-modality use GRIN lens microscopy.

Language: Английский

Citations

0
Point spread function engineering based on wavefront phase modulation DOI
Wei-Qiang Zhang,

Xiaojin Cui,

Junbai Yue

et al.

Published: Nov. 27, 2023

Point spread function (PSF) is the response of an optical system to a point source. The ideal PSF traditional optics Airy pattern corresponding images without aberration. However, shape considered be flexible in computational imaging territory, which can artificially manipulated, and target image restored by reconstruction algorithm. It called engineering improve quality system. method break through limitations even endow systems with new capabilities. In this paper, we will discuss based on wavefront phase modulation, more commonly used for its advantage high throughput. Its application, depth field extension in-situ plankton discussed end-to-end joint optimization, demonstrating defocus invariant quality.

Language: Английский

Citations

0