CD73 in small extracellular vesicles derived from HNSCC defines tumour‐associated immunosuppression mediated by macrophages in the microenvironment DOI
Tingwei Lu, Zhen Zhang, Jianjun Zhang

et al.

Journal of Extracellular Vesicles, Journal Year: 2022, Volume and Issue: 11(5)

Published: May 1, 2022

Abstract Research on tumour cell‐derived small extracellular vesicles (sEVs) that regulate microenvironment (TME) has provided strategies for targeted therapy of head and neck squamous cell carcinoma (HNSCC). Herein, we demonstrated sEVs derived from HNSCC cancer cells carried CD73 (sEVs ), which promoted malignant progression mediated immune evasion. The phagocytosed by tumour‐associated macrophages (TAMs) in the TME induced immunosuppression. Higher high TAMs infiltration levels were correlated with poorer prognosis, while activated NF‐κB pathway TAMs, thereby inhibiting function increasing cytokines secretion such as IL‐6, IL‐10, TNF‐α, TGF‐β1. absence enhanced sensitivity anti‐PD‐1 through reversed Moreover, circulating increased risk lymph node metastasis worse prognosis. Taken together, our study suggests contributes to immunosuppression is a potential predictor responses checkpoint HNSCC.

Language: Английский

Exosomal miR-125b-5p deriving from mesenchymal stem cells promotes tubular repair by suppression of p53 in ischemic acute kidney injury DOI Creative Commons
Jing-Yuan Cao, Bin Wang, Tao‐Tao Tang

et al.

Theranostics, Journal Year: 2021, Volume and Issue: 11(11), P. 5248 - 5266

Published: Jan. 1, 2021

Mesenchymal stem cells-derived exosomes (MSC-exos) have attracted great interest as a cell-free therapy for acute kidney injury (AKI). However, the

Language: Английский

Citations

209
Recent applications of immunomodulatory biomaterials for disease immunotherapy DOI Creative Commons

Huaxing Dai,

Qin Fan, Chao Wang

et al.

Exploration, Journal Year: 2022, Volume and Issue: 2(6)

Published: May 23, 2022

Immunotherapy is used to regulate systemic hyperactivation or hypoactivation treat various diseases. Biomaterial-based immunotherapy systems can improve therapeutic effects through targeted drug delivery, immunoengineering, etc. However, the immunomodulatory of biomaterials themselves cannot be neglected. In this review, we outline with functions discovered in recent years and their applications disease treatment. These inflammation, tumors, autoimmune diseases by regulating immune cell function, exerting enzyme-like activity, neutralizing cytokines, The prospects challenges biomaterial-based modulation are also discussed.

Language: Английский

Citations

200
BMSC-Derived Exosomal miR-29a Promotes Angiogenesis and Osteogenesis DOI Creative Commons
Guo‐Dong Lu, Cheng Peng, Ting Liu

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2020, Volume and Issue: 8

Published: Dec. 9, 2020

Angiogenesis and osteogenesis are tightly coupled during bone modeling remodeling processes. Here we reported that marrow mesenchymal stem cell (BMSC)-derived exosomal miR-29a promotes angiogenesis in vitro vivo . BMSC-derived exosomes (BMSCs-Exos) can be taken up by human umbilical vein endothelial cells (HUVECs) promote the proliferation, migration, tube formation of HUVECs. MiRNA-29a level was high BMSCs-Exos transported into HUVECs to regulate angiogenesis. VASH1 identified as a direct target miR-29a, mediating effects on More interestingly, miR29a-loaded from engineered BMSCs (miR-29a-loaded BMSCs-Exos) showed robust ability promoting Taken together, these findings suggest regulates osteogenesis, miR-29a-loaded may serve potential therapeutic for osteoporosis.

Language: Английский

Citations

151
Extracellular vesicles in kidney disease DOI Open Access
Cristina Grange, Benedetta Bussolati

Nature Reviews Nephrology, Journal Year: 2022, Volume and Issue: 18(8), P. 499 - 513

Published: May 31, 2022

Language: Английский

Citations

119
Lactic acid in macrophage polarization: The significant role in inflammation and cancer DOI
Haicun Zhou,

Xin-yan Yan,

Wenwen Yu

et al.

International Reviews of Immunology, Journal Year: 2021, Volume and Issue: 41(1), P. 4 - 18

Published: July 25, 2021

Metabolite lactic acid has always been regarded as a metabolic by-product rather than bioactive molecule. Recently, this view changed since it was discovered that can be used signal molecule and novel transduction functions both intracellular extracellular, which regulate key in the immune system. In recent years, more evidence shown is closely related to metabolism polarization of macrophages. During inflammation, regulator macrophage metabolism, prevent excessive inflammatory responses; malignant tumors, produced by tumor tissues promotes tumor-associated macrophages, turn progression. review, we examined relationship between metabolism. We further discussed how plays role maintaining homeostasis well biology M1/M2 imbalance human diseases. Potential methods target treatment inflammation cancer will also so provide new strategies for

Language: Английский

Citations

108
Nanodrugs alleviate acute kidney injury: Manipulate RONS at kidney DOI Creative Commons
Qiaohui Chen,

Yayun Nan,

Yuqi Yang

et al.

Bioactive Materials, Journal Year: 2022, Volume and Issue: 22, P. 141 - 167

Published: Sept. 29, 2022

Currently, there are no clinical drugs available to treat acute kidney injury (AKI). Given the high prevalence and mortality rate of AKI, development effectively AKI is a huge unmet medical need research hotspot. Although existing evidence fully demonstrates that reactive oxygen nitrogen species (RONS) burst at site major contributor progression, heterogeneity, complexity, unique physiological structure make most antioxidant anti-inflammatory small molecule ineffective because lack targeting side effects. Recently, nanodrugs with intrinsic through control size, shape, surface properties have opened exciting prospects for treatment AKI. Many emerged address limitations current treatments. In this review, we systematically summarized first time about emerging exploit pathological features overcome traditional small-molecule achieve efficacy. First, analyzed structural characteristics main mechanism AKI: hypoxia, harmful substance accumulation-induced RONS renal despite multifactorial initiation heterogeneity Subsequently, introduced strategies used improve reviewed advances nano-RONS-sacrificial agents, nanozymes, nanocarriers antioxidants drugs. These demonstrated excellent therapeutic effects, such as greatly reducing oxidative stress damage, restoring function, low Finally, discussed challenges future directions translating into treatment.

Language: Английский

Citations

86
Preconditioning and Engineering Strategies for Improving the Efficacy of Mesenchymal Stem Cell-Derived Exosomes in Cell-Free Therapy DOI Creative Commons
Shenyuan Chen, Fengtian Sun, Hui Qian

et al.

Stem Cells International, Journal Year: 2022, Volume and Issue: 2022, P. 1 - 18

Published: May 14, 2022

Mesenchymal stem cells (MSCs) have been widely applied to regenerative medicine owing their multiple differentiation, self-renewal, and immunomodulatory abilities. Exosomes are cell-secreted natural nanovesicles thought be mediators of intercellular communication material transport. The therapeutic potential MSCs can largely attributed MSC-derived exosomes (MSC-exosomes). Emerging evidence suggests that the efficacy MSC-exosomes is highly dependent on status MSCs, optimization extracellular environment affects exosomal content. Pretreatment methods including three-dimensional cultures, hypoxia, other biochemical cues shown potentially enhance biological activity while maintaining or enhancing production. On hand, engineering means desired function has rapidly gaining attention. In particular, biologically active molecule encapsulation membrane modification alter functions targeting MSC-exosomes. this review, we summarize two possible strategies improve MSC-exosomes: preconditioning approaches exosomes. We also explore underlying mechanisms different discuss advantages limitations upcoming clinical applications.

Language: Английский

Citations

75
Inflammation in kidney repair: Mechanism and therapeutic potential DOI
Ying Fu,

Xiang Yu,

Honglin Li

et al.

Pharmacology & Therapeutics, Journal Year: 2022, Volume and Issue: 237, P. 108240 - 108240

Published: July 5, 2022

Language: Английский

Citations

73
Engineering Extracellular Vesicles as Delivery Systems in Therapeutic Applications DOI Creative Commons
Liwei Wang, Di Wang, Zhaoming Ye

et al.

Advanced Science, Journal Year: 2023, Volume and Issue: 10(17)

Published: April 20, 2023

Extracellular vesicles (EVs) are transport secreted by living cells and released into the extracellular environment. Recent studies have shown that EVs serve as "messengers" in intercellular inter-organismal communication, both normal pathological processes. EVs, natural nanocarriers, can deliver bioactivators therapy with their endogenous properties. This review article describes engineering of sources, isolation method, cargo loading, boosting approach, adjustable targeting EVs. Furthermore, summarizes recent progress made EV-based delivery systems applications, including cancer, cardiovascular diseases, liver, kidney, nervous system COVID-19 emphasizes obstacles challenges therapies possible strategies.

Language: Английский

Citations

66
Culture-expanded mesenchymal stromal cell therapy: does it work in knee osteoarthritis? A pathway to clinical success DOI Creative Commons
Griffin Copp, Kevin P. Robb, Sowmya Viswanathan

et al.

Cellular and Molecular Immunology, Journal Year: 2023, Volume and Issue: 20(6), P. 626 - 650

Published: April 25, 2023

Abstract Osteoarthritis (OA) is a degenerative multifactorial disease with concomitant structural, inflammatory, and metabolic changes that fluctuate in temporal patient-specific manner. This complexity has contributed to refractory responses various treatments. MSCs have shown promise as multimodal therapeutics mitigating OA symptoms progression. Here, we evaluated 15 randomized controlled clinical trials (RCTs) 11 nonrandomized RCTs using culture-expanded the treatment of knee OA, found net positive effects on pain (improving function 12/15 relative baseline 11/15 control groups at study endpoints) cartilage protection and/or repair (18/21 studies). We examined MSC dose, tissue origin, autologous vs. allogeneic origins well patient phenotype, endotype, age, sex level severity key parameters parsing effectiveness. The relatively small sample size 610 patients limited drawing definitive conclusions. Nonetheless, noted trends toward moderate higher doses select phenotypes leading structural improvements or preservation. Evidence from preclinical studies supportive anti-inflammatory immunomodulatory effects, but additional investigations immunomodulatory, chondroprotective other mechanisms action are needed. hypothesize basal “fitness” correlates efficacy, this hypothesis needs be validated future studies. conclude roadmap articulating need match an subset defined by molecular endotype phenotype basally “fit” engineered-to-be-fit-for-OA well-designed, data-intensive advance field.

Language: Английский

Citations

52