International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 265, P. 130843 - 130843
Published: March 12, 2024
Language: Английский
Signal Transduction and Targeted Therapy, Journal Year: 2022, Volume and Issue: 7(1)
Published: March 21, 2022
Abstract Mesenchymal stromal/stem cells (MSCs) possess multi-lineage differentiation and self-renewal potentials. MSCs-based therapies have been widely utilized for the treatment of diverse inflammatory diseases, due to potent immunoregulatory functions MSCs. An increasing body evidence indicates that MSCs exert their therapeutic effects largely through paracrine actions. Growth factors, cytokines, chemokines, extracellular matrix components, metabolic products were all found be functional molecules in various paradigms. These secretory factors contribute immune modulation, tissue remodeling, cellular homeostasis during regeneration. In this review, we summarize discuss recent advances our understanding behavior intracellular communication accounts potential treating human diseases.
Language: Английский
Citations
406
Advanced Drug Delivery Reviews, Journal Year: 2022, Volume and Issue: 182, P. 114108 - 114108
Published: Jan. 3, 2022
Language: Английский
Citations
170
Biology, Journal Year: 2022, Volume and Issue: 11(6), P. 804 - 804
Published: May 24, 2022
In recent years, there has been a rapid growth in the knowledge of cell-secreted extracellular vesicle functions. They are membrane enclosed and loaded with proteins, nucleic acids, lipids, other biomolecules. After being released into environment, some these vesicles delivered to recipient cells; consequently, target cell may undergo physiological or pathological changes. Thus, as biological nano-carriers, have pivotal role facilitating long-distance intercellular communication. Understanding mechanisms that mediate this communication process is important not only for basic science but also medicine. Indeed, currently seen immense interest nanomedicine precision medicine their potential use diagnostic, prognostic, therapeutic applications. This paper aims summarize latest advances study smallest subtype among vesicles, exosomes. The article divided several sections, focusing on exosomes' nature, characteristics, commonly used strategies methodologies separation, characterization, visualization. By searching an extended portion relevant literature, work give quick outline extensive nanomedical Moreover, considerations require further investigations before translating them clinical applications summarized.
Language: Английский
Citations
169
International Journal of Nanomedicine, Journal Year: 2022, Volume and Issue: Volume 17, P. 1757 - 1781
Published: April 1, 2022
Abstract: Inflammation is a beneficial and physiological process, but there are number of inflammatory diseases which have detrimental effects on the body. In addition, drugs used to treat inflammation toxic side when over long period time. Mesenchymal stem cells (MSCs) pluripotent that can be isolated from variety tissues differentiate into diverse cell types under appropriate conditions. They also exhibit noteworthy anti-inflammatory properties, providing new options for treatment diseases. The therapeutic potential MSCs currently being investigated various diseases, such as kidney injury, lung osteoarthritis (OA), rheumatoid arthritis (RA), bowel disease (IBD). perform multiple functions, including immunomodulation, homing, differentiation, enable damaged form balanced regenerative microenvironment severe accumulated evidence indicates exosomes extracellular vesicles (MSC-Exos) play an extraordinary role, mainly by transferring their components recipient cells. this review, we summarize mechanism clinical trials MSC-Exos in detail, with view contributing Keywords: mesenchymal cells, exosome, clinic data, drug delivery, targeted therapy
Language: Английский
Citations
102
Bioactive Materials, Journal Year: 2022, Volume and Issue: 22, P. 141 - 167
Published: Sept. 29, 2022
Currently, there are no clinical drugs available to treat acute kidney injury (AKI). Given the high prevalence and mortality rate of AKI, development effectively AKI is a huge unmet medical need research hotspot. Although existing evidence fully demonstrates that reactive oxygen nitrogen species (RONS) burst at site major contributor progression, heterogeneity, complexity, unique physiological structure make most antioxidant anti-inflammatory small molecule ineffective because lack targeting side effects. Recently, nanodrugs with intrinsic through control size, shape, surface properties have opened exciting prospects for treatment AKI. Many emerged address limitations current treatments. In this review, we systematically summarized first time about emerging exploit pathological features overcome traditional small-molecule achieve efficacy. First, analyzed structural characteristics main mechanism AKI: hypoxia, harmful substance accumulation-induced RONS renal despite multifactorial initiation heterogeneity Subsequently, introduced strategies used improve reviewed advances nano-RONS-sacrificial agents, nanozymes, nanocarriers antioxidants drugs. These demonstrated excellent therapeutic effects, such as greatly reducing oxidative stress damage, restoring function, low Finally, discussed challenges future directions translating into treatment.
Language: Английский
Citations
86
Stem Cells International, Journal Year: 2022, Volume and Issue: 2022, P. 1 - 18
Published: May 14, 2022
Mesenchymal stem cells (MSCs) have been widely applied to regenerative medicine owing their multiple differentiation, self-renewal, and immunomodulatory abilities. Exosomes are cell-secreted natural nanovesicles thought be mediators of intercellular communication material transport. The therapeutic potential MSCs can largely attributed MSC-derived exosomes (MSC-exosomes). Emerging evidence suggests that the efficacy MSC-exosomes is highly dependent on status MSCs, optimization extracellular environment affects exosomal content. Pretreatment methods including three-dimensional cultures, hypoxia, other biochemical cues shown potentially enhance biological activity while maintaining or enhancing production. On hand, engineering means desired function has rapidly gaining attention. In particular, biologically active molecule encapsulation membrane modification alter functions targeting MSC-exosomes. this review, we summarize two possible strategies improve MSC-exosomes: preconditioning approaches exosomes. We also explore underlying mechanisms different discuss advantages limitations upcoming clinical applications.
Language: Английский
Citations
75
International Journal of Nanomedicine, Journal Year: 2023, Volume and Issue: Volume 18, P. 7923 - 7940
Published: Dec. 1, 2023
Exosomes are nano-sized membrane vesicles that transfer bioactive molecules between cells and modulate various biological processes under physiological pathological conditions. By applying bioengineering technologies, exosomes can be modified to express specific markers or carry therapeutic cargo emerge as novel platforms for the treatment of cancer, neurological, cardiovascular, immune, infectious diseases. However, there many challenges uncertainties in clinical translation exosomes. This review aims provide an overview recent advances engineered exosomes, with a special focus on methods strategies loading drugs into pros cons different methods, optimization exosome production based encapsulated. Moreover, we also summarize current applications prospects well potential risks limitations need addressed engineering, including standardization preparation engineering protocols, quality quantity control drug release, immunogenicity cytotoxicity Overall, represent exciting frontier nanomedicine, but they still face large-scale production, maintenance storage stability, translation. With continuous this field, exosome-based formulation could offer great promise targeted human
Language: Английский
Citations
61
Periodontology 2000, Journal Year: 2024, Volume and Issue: 94(1), P. 257 - 414
Published: Feb. 1, 2024
Abstract Exosomes are the smallest subset of extracellular signaling vesicles secreted by most cells with ability to communicate other tissues and cell types over long distances. Their use in regenerative medicine has gained tremendous momentum recently due their be utilized as therapeutic options for a wide array diseases/conditions. Over 5000 publications currently being published yearly on this topic, number is only expected dramatically increase novel strategies continue developed. Today exosomes have been applied numerous contexts including neurodegenerative disorders (Alzheimer's disease, central nervous system, depression, multiple sclerosis, Parkinson's post‐traumatic stress disorders, traumatic brain injury, peripheral nerve injury), damaged organs (heart, kidney, liver, stroke, myocardial infarctions, ovaries), degenerative processes (atherosclerosis, diabetes, hematology musculoskeletal degeneration, osteoradionecrosis, respiratory disease), infectious diseases (COVID‐19, hepatitis), procedures (antiaging, bone regeneration, cartilage/joint osteoarthritis, cutaneous wounds, dental dermatology/skin erectile dysfunction, hair regrowth, intervertebral disc repair, spinal cord vascular regeneration), cancer therapy (breast, colorectal, gastric osteosarcomas), immune function (allergy, autoimmune regulation, inflammatory diseases, lupus, rheumatoid arthritis). This scoping review first its kind aimed at summarizing extensive potential broad range disorders.
Language: Английский
Citations
19
Frontiers in Immunology, Journal Year: 2021, Volume and Issue: 12
Published: July 14, 2021
Recent studies have shown that autophagy upregulation can attenuate sepsis-induced acute kidney injury (SAKI). The tumor suppressor p53 has emerged as an regulator in various forms of (AKI). Our previous showed acetylation exacerbated hemorrhagic shock-induced AKI and lipopolysaccharide (LPS)-induced endothelial barrier dysfunction. However, the role p53-regulated SAKI not been examined requires clarification. In this study, we observed dynamic changes renal tubular epithelial cells (RTECs) verified protective effects activation on SAKI. We also protein expression, intracellular distribution (nuclear cytoplasmic), acetylation/deacetylation levels during following cecal ligation puncture (CLP) or LPS treatment mice a LPS-challenged human RTEC cell line (HK-2 cells). After sepsis stimulation, RTECs increased temporarily, followed by sharp decrease. Autophagy inhibition was accompanied score. By contrast, agonists could reduce damage sepsis. Surprisingly, expression both cortex HK-2 did significantly change stimulation. translocation from nucleus to cytoplasm increased, enhanced. mechanistic found induction deacetylation, due either resveratrol/quercetin -induced deacetylase Sirtuin 1 (Sirt1) mutation acetylated lysine site p53, promoted alleviated addition, easier bind with Beclin1 accelerated its ubiquitination-mediated degradation. study underscores importance deacetylated p53-mediated future treatments.
Language: Английский
Citations
105
Bioactive Materials, Journal Year: 2022, Volume and Issue: 17, P. 344 - 359
Published: Jan. 22, 2022
Shoulder stiffness (SS) is a common shoulder disease characterized by increasing pain and limited range of motion. SS considered to be an inflammatory fibrotic disorder pathologically. However, there no consensus on the most effective conservative treatment for fibrosis. Given that human Bone Marrow Mesenchymal Stem Cell-derived extracellular vesicles (BMSC-EVs) displayed promising therapeutic effects various tissues, we investigated effect BMSC-EVs fibrosis in mice immobilization model two cell models. By conducting series experiments, found can significantly inhibit fibrogenic process both vitro vivo. In detail, suppressed aberrant proliferation, high collagen production capacity, activation pathways TGF-β-stimulated fibroblasts vitro. Besides, vivo, reduced infiltration, tissue capsule, improved mobility. addition, via exosomal small RNA sequencing qPCR analysis, let-7a-5p was verified highest expressed miRNA with predicted antifibrotic capability BMSC-EVs. The capacity impaired after knockdown let-7a-5p. Moreover, discovered mRNA TGFBR1 (the membrane receptor transforming growth factor β) target Together, these findings elucidated role capsular This study clarifies new approach using stem cell-derived EVs therapy as alternative therapy, which may clinically benefit patients future.
Language: Английский
Citations
65