Large-scale two-photon calcium imaging in freely moving mice

Cell, Journal Year: 2022, Volume and Issue: 185(7), P. 1240 - 1256.e30

Published: March 1, 2022

We developed a miniaturized two-photon microscope (MINI2P) for fast, high-resolution, multiplane calcium imaging of over 1,000 neurons at time in freely moving mice. With weight below 3 g and highly flexible connection cable, MINI2P allowed stable with no impediment behavior variety assays compared to untethered, unimplanted animals. The improved cell yield was achieved through optical system design featuring an enlarged field view (FOV) microtunable lens increased z-scanning range speed that allows fast multiple interleaved planes, as well 3D functional imaging. Successive across multiple, adjacent FOVs enabled recordings from more than 10,000 the same animal. Large-scale proof-of-principle data were obtained populations visual cortex, medial entorhinal hippocampus, revealing spatial tuning cells all areas.

Language: Английский

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High-resolution Fourier light-field microscopy for volumetric multi-color live-cell imaging

Optica, Journal Year: 2021, Volume and Issue: 8(5), P. 614 - 614

Published: March 18, 2021

Volumetric interrogation of the organization and processes intracellular organelles molecules in cellular systems with a high spatiotemporal resolution is essential for understanding cell physiology, development, pathology. Here, we report high-resolution Fourier light-field microscopy (HR-FLFM) fast volumetric live-cell imaging. HR-FLFM transforms conventional enables exploration less accessible spatiotemporal-limiting regimes single-cell studies. The results present near-diffraction-limited all three dimensions, five-fold extended focal depth to several micrometers, scanning-free volume acquisition time up milliseconds. system demonstrates instrumentation accessibility, low photo damage continuous observation, compatibility general assays. We anticipate offer promising methodological pathway investigating wide range functions exquisite contextual details.

Language: Английский

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Multiregion neuronal activity: the forest and the trees

Nature reviews. Neuroscience, Journal Year: 2022, Volume and Issue: 23(11), P. 683 - 704

Published: Oct. 3, 2022

Language: Английский

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Deep-learning-augmented computational miniature mesoscope

Optica, Journal Year: 2022, Volume and Issue: 9(9), P. 1009 - 1009

Published: Aug. 3, 2022

Fluorescence microscopy is essential to study biological structures and dynamics. However, existing systems suffer from a tradeoff between field-of-view (FOV), resolution, complexity, thus cannot fulfill the emerging need of miniaturized platforms providing micron-scale resolution across centimeter-scale FOVs. To overcome this challenge, we developed Computational Miniature Mesoscope (CM$^2$) that exploits computational imaging strategy enable single-shot 3D high-resolution wide FOV in platform. Here, present CM$^2$ V2 significantly advances both hardware computation. We complement 3$\times$3 microlens array with new hybrid emission filter improves contrast by 5$\times$, design 3D-printed freeform collimator for LED illuminator excitation efficiency 3$\times$. reconstruction large volume, develop an accurate efficient linear shift-variant (LSV) model characterizes spatially varying aberrations. then train multi-module deep learning model, CM$^2$Net, using only 3D-LSV simulator. show CM$^2$Net generalizes well experiments achieves $\sim$7-mm 800-$\mu$m depth, provides $\sim$6-$\mu$m lateral $\sim$25-$\mu$m axial resolution. This $\sim$8$\times$ better localization $\sim$1400$\times$ faster speed as compared previous model-based algorithm. anticipate simple low-cost miniature system will be impactful many large-scale fluorescence applications.

Language: Английский

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An optical design enabling lightweight and large field-of-view head-mounted microscopes

Nature Methods, Journal Year: 2023, Volume and Issue: 20(4), P. 546 - 549

Published: March 16, 2023

Language: Английский

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Mesoscale volumetric light-field (MesoLF) imaging of neuroactivity across cortical areas at 18 Hz

Nature Methods, Journal Year: 2023, Volume and Issue: 20(4), P. 600 - 609

Published: Feb. 23, 2023

Language: Английский

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Random-access wide-field mesoscopy for centimetre-scale imaging of biodynamics with subcellular resolution

Nature Photonics, Journal Year: 2024, Volume and Issue: 18(7), P. 721 - 730

Published: April 17, 2024

Abstract Benefitting from the advantages of high imaging throughput and low cost, wide-field microscopy has become indispensable in biomedical studies. However, it remains challenging to record biodynamics with a large field view spatiotemporal resolution due limited space–bandwidth product. Here we propose random-access (RA-WiFi) mesoscopy for vivo over 163.84 mm 2 area spatial ~2.18 μm. We extend beyond nominal value objective by enlarging object distance, which leads lower angle, followed correction optical aberrations. also implement scanning structured illumination, enables optical-sectioning capability contrast. The multi-plane makes technique suitable curved-surface samples. demonstrate RA-WiFi multi-modal imaging, including bright-field, dark-field multi-colour fluorescence imaging. Specifically, apply calcium cortex-wide neural network activities awake mice vivo, under both physiological pathological conditions. show its unique three-dimensional random access irregular regions interest via biodynamic mouse spinal cords vivo. As compact, low-cost mesoscope capability, will enable broad applications study biological systems.

Language: Английский

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Long-term mesoscale imaging of 3D intercellular dynamics across a mammalian organ

Cell, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 1, 2024

Language: Английский

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Neurophotonic Tools for Microscopic Measurements and Manipulation: Status Report

Neurophotonics, Journal Year: 2022, Volume and Issue: 9(S1)

Published: April 27, 2022

Neurophotonics was launched in 2014 coinciding with the launch of BRAIN Initiative focused on development technologies for advancement neuroscience. For last seven years, Neurophotonics' agenda has been well aligned this focus neurotechnologies featuring new optical methods and tools applicable to brain studies. While 2.0 is pivoting towards applications these novel quest understand brain, article we review an extensive diverse toolkit explore function that have emerged from related large-scale efforts measurement manipulation structure function. Here, neurophotonic mostly animal A companion article, scheduled appear later year, will cover diffuse imaging noninvasive human each domain, outline current state-of-the-art respective technologies, identify areas where innovation needed provide outlook future directions.

Language: Английский

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Large depth-of-field ultra-compact microscope by progressive optimization and deep learning

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: July 11, 2023

The optical microscope is customarily an instrument of substantial size and expense but limited performance. Here we report integrated that achieves performance beyond a commercial with 5×, NA 0.1 objective only at 0.15 cm3 0.5 g, whose five orders magnitude smaller than conventional microscope. To achieve this, progressive optimization pipeline proposed which systematically optimizes both aspherical lenses diffractive elements over 30 times memory reduction compared to the end-to-end optimization. By designing simulation-supervision deep neural network for spatially varying deconvolution during design, accomplish 10 improvement in depth-of-field traditional microscopes great generalization wide variety samples. show unique advantages, equipped cell phone without any accessories application portable diagnostics. We believe our method provides new framework design miniaturized high-performance imaging systems by integrating optics, computational learning.

Language: Английский

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