International Communications in Heat and Mass Transfer, Journal Year: 2024, Volume and Issue: 159, P. 108265 - 108265
Published: Oct. 24, 2024
Language: Английский
Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(3)
Published: Jan. 8, 2024
Plastics are now omnipresent in our daily lives. The existence of microplastics (1 µm to 5 mm length) and possibly even nanoplastics (<1 μm) has recently raised health concerns. In particular, believed be more toxic since their smaller size renders them much amenable, compared microplastics, enter the human body. However, detecting imposes tremendous analytical challenges on both nano-level sensitivity plastic-identifying specificity, leading a knowledge gap this mysterious nanoworld surrounding us. To address these challenges, we developed hyperspectral stimulated Raman scattering (SRS) imaging platform with an automated plastic identification algorithm that allows micro-nano analysis at single-particle level high chemical specificity throughput. We first validated enhancement narrow band SRS enable high-speed single nanoplastic detection below 100 nm. then devised data-driven spectral matching imposed by sensitive narrow-band achieve robust determination common polymers. With established technique, studied plastics from bottled water as model system. successfully detected identified major types. Micro-nano concentrations were estimated about 2.4 ± 1.3 × 10
Language: Английский
Citations
174
Nature Photonics, Journal Year: 2023, Volume and Issue: 17(4), P. 330 - 337
Published: Jan. 23, 2023
Language: Английский
Citations
51
Laser & Photonics Review, Journal Year: 2023, Volume and Issue: 17(12)
Published: Oct. 30, 2023
Abstract Label‐free super‐resolution (LFSR) imaging relies on light‐scattering processes in nanoscale objects without a need for fluorescent (FL) staining required super‐resolved FL microscopy. The objectives of this Roadmap are to present comprehensive vision the developments, state‐of‐the‐art field, and discuss resolution boundaries hurdles that be overcome break classical diffraction limit label‐free imaging. scope spans from advanced interference detection techniques, where diffraction‐limited lateral is combined with unsurpassed axial temporal resolution, techniques true capability based understanding as an information science problem, using novel structured illumination, near‐field scanning, nonlinear optics approaches, designing superlenses nanoplasmonics, metamaterials, transformation optics, microsphere‐assisted approaches. To end, brings under same umbrella researchers physics biomedical communities which such studies have often been developing separately. ultimate intent paper create current future developments LFSR its physical mechanisms great opening series articles field.
Language: Английский
Citations
49
Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)
Published: Jan. 8, 2024
Abstract Understanding metabolic heterogeneity is the key to uncovering underlying mechanisms of metabolic-related diseases. Current imaging studies suffer from limitations including low resolution and specificity, model systems utilized often lack human relevance. Here, we present a single-cell platform enable direct lipid metabolism with high specificity in various human-derived 2D 3D culture systems. Through incorporation an azide-tagged infrared probe, selective detection newly synthesized lipids cells tissue became possible, while simultaneous fluorescence enabled cell-type identification complex tissues. In proof-of-concept experiments, were directly visualized human-relevant among different cell types, mutation status, differentiation stages, over time. We identified upregulated progranulin-knockdown induced pluripotent stem their differentiated microglia cells. Furthermore, observed that neurons brain organoids exhibited significantly lower compared astrocytes.
Language: Английский
Citations
24
Journal of Semiconductors, Journal Year: 2025, Volume and Issue: 46(1), P. 011602 - 011602
Published: Jan. 1, 2025
Abstract Infrared optoelectronic sensing is the core of many critical applications such as night vision, health and medication, military, space exploration, etc. Further including mechanical flexibility a new dimension enables novel features adaptability conformability, promising for developing next-generation sensory toward reduced size, weight, price, power consumption, enhanced performance (SWaP 3 ). However, in this emerging research frontier, challenges persist simultaneously achieving high infrared response good deformability devices integrated systems. Therefore, we perform comprehensive review design strategies insights flexible sensors, fundamentals photodetectors, selection materials device architectures, fabrication techniques strategies, discussion architectural functional integration towards wearable optoelectronics advanced image sensing. Finally, article offers into future directions to practically realize ultra-high smart sensors enabled by infrared-sensitive materials, covering development micro-/nanofabrication. Benchmarks scaling these across fabrication, performance, are presented, alongside perspectives on potential medication health, biomimetic neuromorphic systems,
Language: Английский
Citations
4
Science, Journal Year: 2021, Volume and Issue: 374(6572), P. 1264 - 1267
Published: Dec. 2, 2021
Optomechanical upconversion Molecules have rich signatures in their spectra at infrared wavelengths and are typically accessed with dedicated spectroscopic instrumentation. Chen et al . Xomalis report optomechanical frequency from the mid-infrared to visible domain using molecular vibrations coupled a plasmonic nanocavity ambient conditions (see Perspective by Gordon). Using different nanoantenna designs, one nanoparticle-on-resonator other nanoparticle-in-groove, both approaches show ability upconvert of molecules light wavelengths. The effect could be used simplify spectroscopy, possibly single-molecule sensitivity. —ISO
Language: Английский
Citations
95
Chemical Reviews, Journal Year: 2021, Volume and Issue: 121(19), P. 11937 - 11970
Published: Sept. 29, 2021
Our ability to detect, image, and quantify nanoscopic objects molecules with visible light has undergone dramatic improvements over the past few decades. While fluorescence historically been go-to contrast mechanism for ultrasensitive microscopy due its superior background suppression specificity, recent developments based on scattering have reached single-molecule sensitivity. They also advantages of universal applicability obtain information about species interest beyond presence location. Many advances are driven by novel approaches illumination, detection, suppression, all aimed at isolating maximizing signal interest. Here, we review these grouped according basic principles used, namely darkfield imaging, interferometric surface plasmon resonance microscopy.
Language: Английский
Citations
92
The Journal of Physical Chemistry B, Journal Year: 2022, Volume and Issue: 126(43), P. 8597 - 8613
Published: Oct. 26, 2022
Midinfrared photothermal (MIP) microscopy, also called optical infrared (O-PTIR) is an emerging tool for bond-selective chemical imaging of living biological and material samples. In MIP a visible probe beam detects the photothermal-based contrast induced by vibrational absorption. With submicron spatial resolution, high spectral fidelity, reduced water absorption background, microscopy has overcome limitations in methods. this review, we summarize basic principle different origins contrasts, recent technology development that pushed speed, sensitivity to new stage. We further emphasize its broad applications life science characterization, provide perspective future technical advances.
Language: Английский
Citations
61
Annual Review of Analytical Chemistry, Journal Year: 2023, Volume and Issue: 16(1), P. 205 - 230
Published: April 17, 2023
Infrared (IR) spectroscopic imaging records spatially resolved molecular vibrational spectra, enabling a comprehensive measurement of the chemical makeup and heterogeneity biological tissues. Combining this novel contrast mechanism in microscopy with use artificial intelligence can transform practice histopathology, which currently relies largely on human examination morphologic patterns within stained tissue. First, review summarizes IR instrumentation especially suited to analyses its performance, major trends. Second, an overview data processing methods application machine learning is given, emphasis emerging deep learning. Third, discussion workflows pathology provided, four categories proposed based complexity analytical performance needed. Last, set guidelines, termed experimental specifications for are help standardize diversity approaches area.
Language: Английский
Citations
35
Science Advances, Journal Year: 2023, Volume and Issue: 9(24)
Published: June 14, 2023
By optically sensing absorption-induced photothermal effect, mid-infrared (IR) (MIP) microscope enables super-resolution IR imaging of biological systems in water. However, the speed current sample-scanning MIP system is limited to milliseconds per pixel, which insufficient for capturing living dynamics. detecting transient signal induced by a single pulse through fast digitization, we report laser-scanning that increases three orders magnitude. To realize single-pulse detection, use synchronized galvo scanning both mid-IR and probe beams achieve an line rate more than 2 kilohertz. With video-rate speed, observed dynamics various biomolecules organisms at multiple scales. Furthermore, using hyperspectral imaging, chemically dissected layered ultrastructure fungal cell wall. Last, with uniform field view 200 square micrometer, mapped fat storage free-moving
Language: Английский
Citations
33