Sustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopy

Cell, Journal Year: 2022, Volume and Issue: 185(18), P. 3408 - 3425.e29

Published: Aug. 18, 2022

Genetically encoded voltage indicators are emerging tools for monitoring dynamics with cell-type specificity. However, current enable a narrow range of applications due to poor performance under two-photon microscopy, method choice deep-tissue recording. To improve indicators, we developed multiparameter high-throughput platform optimize microscopy. Using this system, identified JEDI-2P, an indicator that is faster, brighter, and more sensitive photostable than its predecessors. We demonstrate JEDI-2P can report light-evoked responses in axonal termini Drosophila interneurons the dendrites somata amacrine cells isolated mouse retina. also optically record individual cortical neurons awake behaving mice 30 min using both resonant-scanning ULoVE random-access Finally, recording robustly detect spikes at depths exceeding 400 μm correlations pairs neurons.

Language: Английский

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Evolution of central neural circuits: state of the art and perspectives

Nature reviews. Neuroscience, Journal Year: 2022, Volume and Issue: 23(12), P. 725 - 743

Published: Oct. 26, 2022

Language: Английский

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Ancestral photoreceptor diversity as the basis of visual behaviour

Nature Ecology & Evolution, Journal Year: 2024, Volume and Issue: 8(3), P. 374 - 386

Published: Jan. 22, 2024

Language: Английский

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Morphology and connectivity of retinal horizontal cells in two avian species

Frontiers in Cellular Neuroscience, Journal Year: 2025, Volume and Issue: 19

Published: March 4, 2025

In the outer vertebrate retina, visual signal is separated into intensity and wavelength information. birds, seven types of photoreceptors (one rod, four single cones, two members double cone) mediate signals to >20 second-order neurons, bipolar cells horizontal cells. Horizontal contribute color contrast processing by providing feedback feedforward fish, reptiles, amphibians they either encode or show color-opponent responses. Yet, for bird number cell not fully resolved even more importantly, synapses between have never been quantified any species. With a combination light microscopy serial EM reconstructions, we found different in distantly related species, domestic chicken European robin. agreement with some earlier studies, confirmed highly abundant (H1, H2) rare (H3, H4), which H1 an axon-bearing cell, whereas H2-H4 are axonless. made chemical one type interplexiform amacrine at their soma. Dendritic contacts H1-H4 were type-specific similar turtle confirms high degree evolutionary conservation retina. Our data further suggests that potentially H2 may intensity, H3 H4 represent opponent birds’ superb and/or acuity vision.

Language: Английский

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More than double the fun with two-photon excitation microscopy

Communications Biology, Journal Year: 2024, Volume and Issue: 7(1)

Published: March 26, 2024

Abstract For generations researchers have been observing the dynamic processes of life through lens a microscope. This has offered tremendous insights into biological phenomena that span multiple orders time- and length-scales ranging from pure magic molecular reorganization at membrane immune cells, to cell migration differentiation during development or wound healing. Standard fluorescence microscopy techniques offer glimpses such in vitro, however, when applied intact systems, they are challenged by reduced signal strengths signal-to-noise ratios result deeper imaging. As remedy, two-photon excitation (TPE) takes special place, because it allows us investigate vivo, their natural environment, even living animal. Here, we review fundamental principles underlying TPE aimed basic advanced users interested adopting for intravital We focus on applications neurobiology, present current trends towards faster, wider imaging, discuss combination with photon counting technologies metabolic imaging spectroscopy, as well highlight outstanding issues drawbacks application these methodologies.

Language: Английский

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From water to land: Evolution of photoreceptor circuits for vision in air

PLoS Biology, Journal Year: 2024, Volume and Issue: 22(1), P. e3002422 - e3002422

Published: Jan. 22, 2024

When vertebrates first conquered the land, they encountered a visual world that was radically distinct from of their aquatic ancestors. Fish exploit strong wavelength-dependent interactions light with water by differentially feeding signals up to 5 spectral photoreceptor types into behavioural programmes. However, above same rules do not apply, and this called for an update circuit strategies. Early tetrapods soon evolved double cone, still poorly understood pair new photoreceptors brought “ancestral terrestrial” complement 7. Subsequent nonmammalian lineages adapted highly parallelised retinal input strategy diverse ecologies. By contrast, mammals shed most ancestral converged on is exceptionally general. In eutherian including in humans, parallelisation emerges gradually as signal traverses layers retina brain.

Language: Английский

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Ancient origin of the rod bipolar cell pathway in the vertebrate retina

Nature Ecology & Evolution, Journal Year: 2024, Volume and Issue: 8(6), P. 1165 - 1179

Published: April 16, 2024

Language: Английский

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Ultraviolet vision in anemonefish improves colour discrimination

Journal of Experimental Biology, Journal Year: 2024, Volume and Issue: 227(7)

Published: April 1, 2024

ABSTRACT In many animals, ultraviolet (UV) vision guides navigation, foraging, and communication, but few studies have addressed the contribution of UV signals to colour vision, or measured discrimination thresholds using behavioural experiments. Here, we tested in an anemonefish (Amphiprion ocellaris) a five-channel (RGB-V-UV) LED display. We first determined that maximal sensitivity A. ocellaris cone was ∼386 nm microspectrophotometry. Three additional spectral sensitivities had maxima at ∼497, 515 ∼535 nm. then behaviourally by training distinguish coloured target pixel from grey distractor pixels varying intensity. Thresholds were calculated for nine sets colours with without signals. Using tetrachromatic model, found better (i.e. lower) discriminating when higher chromatic contrast. These caused greater stimulation relative other types. findings imply component cues improves their detectability, which likely increases prominence body patterns communication silhouette zooplankton prey.

Language: Английский

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Birds multiplex spectral and temporal visual information via retinal On- and Off-channels

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Aug. 31, 2023

In vertebrate vision, early retinal circuits divide incoming visual information into functionally opposite elementary signals: On and Off, transient sustained, chromatic achromatic. Together these signals can yield an efficient representation of the scene for transmission to brain via optic nerve. However, this long-standing interpretation function is based on mammals, it unclear whether functional arrangement common all vertebrates. Here we show that male poultry chicks use a fundamentally different strategy communicate from eye brain. Rather than using pairs output channels, encode polarity, timing, spectral composition stimuli in highly correlated manner: fast achromatic encoded by Off-circuits, slow overwhelmingly On-circuits. Moreover, most channels combine On- Off-circuits simultaneously encode, or multiplex, both information. Our results birds conform evidence fish, amphibians, reptiles which retain full ancestral complement four types cone photoreceptors.

Language: Английский

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Transcription factors underlying photoreceptor diversity

eLife, Journal Year: 2023, Volume and Issue: 12

Published: Feb. 6, 2023

During development, retinal progenitors navigate a complex landscape of fate decisions to generate the major cell classes necessary for proper vision. Transcriptional regulation is critical diversity within these classes. Here, we aim provide resources and techniques required identify transcription factors maintain in photoreceptor subtypes, which are First, key resource: high-quality deep transcriptomic profile each subtype adult zebrafish. We make this resource openly accessible, easy explore, have integrated it with other currently available datasets. Second, using our profiles, derive an in-depth map expression photoreceptors. Third, use efficient CRISPR-Cas9 based mutagenesis screen null phenotypes F0 larvae (F0 screening) as fast, efficient, versatile technique assess involvement candidate generation subtypes. first show that known can be easily replicated method: loss S cones foxq2 mutants rods nr2e3 mutants. then novel functions factor Tbx2, demonstrating plays distinct roles controlling all subtypes retina. Our study provides roadmap discover additional involved process. Additionally, explore four unknown function (Skor1a, Sall1a, Lrrfip1a, Xbp1), find no evidence their This dataset screening method will valuable way genes many essential aspects biology.

Language: Английский

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