Bacterial therapies at the interface of synthetic biology and nanomedicine

Nature Reviews Bioengineering, Journal Year: 2023, Volume and Issue: 2(2), P. 120 - 135

Published: Oct. 10, 2023

Language: Английский

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Novel mechanism by which extracellular vesicles derived from Lactobacillus murinus alleviates deoxynivalenol-induced intestinal barrier disruption

Environment International, Journal Year: 2024, Volume and Issue: 185, P. 108525 - 108525

Published: Feb. 22, 2024

Deoxynivalenol (DON) is a common environmental pollutant that poses serious health risk to humans worldwide. This study was aim explore whether gut microbiota involved in DON-induced intestinal toxicity as well reveal effect of probiotics derived from protecting barrier and elucidate mechanism. We found DON caused disturbed microbiota, particularly Lactobacillus murinus (L. murinus) deficiency. enhanced M1 macrophage polarization decreased tight junction protein expression. Microbiota transplantation experiments showed transfer DON-disrupted healthy mice resulted delivery toxicity. Besides, lost its damaging on antibiotic-treated mice. Further intervention revealed L. induce conversion M2 phenotype through secreted extracellular vesicles (EVs) alleviate disruption. Mechanistically, EVs activate TLR2 promote release IL-10, which turn enhances function. Upon successful translation efficacy into clinical practice, created could be novel possible treatment strategy for disease.

Language: Английский

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A Gas Therapy Strategy for Intestinal Flora Regulation and Colitis Treatment by Nanogel‐Based Multistage NO Delivery Microcapsules

Advanced Materials, Journal Year: 2024, Volume and Issue: 36(19)

Published: Feb. 7, 2024

Current approaches to treating inflammatory bowel disease focus on the suppression of overactive immune responses, removal reactive intestinal oxygen species, and regulation flora. However, owing complex structure gastrointestinal tract influence mucus, current small-molecule biologic-based drugs for colitis cannot effectively act at site colon inflammation, as a result, they tend exhibit low efficacies toxic side effects. In this study, nanogel-based multistage NO delivery microcapsules are developed achieve release inflammation by targeting tissues using nanogel. Surprisingly, oral administration suppresses growth pathogenic bacteria increases abundance probiotic bacteria. Metabolomics further show that an increased probiotics promotes production metabolites, including short-chain fatty acids indole derivatives, which modulate immunity restore barrier via interleukin-17 PI3K-Akt signaling pathways. This work reveals gas therapy strategy based modulates microbial balance, thereby reducing promoting repair, ultimately providing new therapeutic approach clinical management colitis.

Language: Английский

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Engineered bacterial outer membrane vesicles: a versatile bacteria-based weapon against gastrointestinal tumors

Theranostics, Journal Year: 2024, Volume and Issue: 14(2), P. 761 - 787

Published: Jan. 1, 2024

Outer membrane vesicles (OMVs) are nanoscale lipid bilayer structures released by gram-negative bacteria.They share composition and properties with their originating cells, making them adept at traversing cellular barriers.These OMVs have demonstrated exceptional stability, immunogenicity, safety, penetration, tumor-targeting properties, which been leveraged in developing vaccines drug delivery systems.Recent research efforts focused on engineering to increase production yield, reduce cytotoxicity, improve the safety efficacy of treatment.Notably, gastrointestinal (GI) tumors proven resistant several traditional oncological treatment strategies, including chemotherapy, radiotherapy, targeted therapy.Although immune checkpoint inhibitors some patients, usage as monotherapy remains limited tumor heterogeneity individual variability.The immunogenic modifiable nature makes an ideal design platform for individualized GI tumors.OMV-based therapy enables combination optimization anti-tumor effects.This review comprehensively summarizes recent advances OMV discusses challenges clinical translation emerging OMV-based therapies.

Language: Английский

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Extracellular vesicles derived from Lactobacillus johnsonii promote gut barrier homeostasis by enhancing M2 macrophage polarization

Journal of Advanced Research, Journal Year: 2024, Volume and Issue: unknown

Published: March 1, 2024

Diarrheic disease is a common intestinal health problem worldwide, causing great suffering to humans and animals. Precise manipulation strategies based on probiotics combat diarrheic diseases have not been fully developed.

Language: Английский

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Akkermansia muciniphila alleviates high‐fat‐diet‐related metabolic‐associated fatty liver disease by modulating gut microbiota and bile acids

Microbial Biotechnology, Journal Year: 2023, Volume and Issue: 16(10), P. 1924 - 1939

Published: June 28, 2023

It has been reported that Akkermansia muciniphila improves host metabolism and reduces inflammation; however, its potential effects on bile acid metabolic patterns in metabolic-associated fatty liver disease (MAFLD) are unknown. In this study, we have analysed C57BL/6 mice under three feeding conditions: (i) a low-fat diet group (LP), (ii) high-fat (HP) (iii) supplemented with A. (HA). The results found administration relieved weight gain, hepatic steatosis injury induced by the diet. altered gut microbiota decrease Alistipes, Lactobacilli, Tyzzerella, Butyricimonas Blautia, an enrichment of Ruminiclostridium, Osclibacter, Allobaculum, Anaeroplasma Rikenella. changes correlated significantly acids. Meanwhile, also improved glucose tolerance, barriers adipokines dysbiosis. regulated intestinal FXR-FGF15 axis reshaped construction acids, reduced secondary acids caecum liver, including DCA LCA. These findings provide new insights into relationships between probiotics, microflora disorders, highlighting role management MAFLD.

Language: Английский

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Effect of Extracelluar Vesicles Derived from Akkermansia muciniphila on Intestinal Barrier in Colitis Mice

Nutrients, Journal Year: 2023, Volume and Issue: 15(22), P. 4722 - 4722

Published: Nov. 8, 2023

Inflammatory bowel disease (IBD) is a chronic and recurrent disease. It has been observed that the incidence prevalence of IBD are increasing, which consequently raises risk developing colon cancer. Recently, regulation intestinal barrier by probiotics become an effective treatment for colitis. Akkermansia muciniphila-derived extracellular vesicles (Akk EVs) nano-vesicles contain multiple bioactive macromolecules with potential to modulate barrier. In this study, we used ultrafiltration in conjunction high-speed centrifugation extract Akk EVs. A lipopolysaccharide (LPS)-induced RAW264.7 cell model was established assess anti-inflammatory effects found EVs were able be absorbed cells significantly reduce expression nitric oxide (NO), TNF-α, IL-1β (p < 0.05). We explored preventative on colitis regulating using mouse caused dextran sulfate sodium (DSS). The findings demonstrated effectively prevented symptoms reduced colonic tissue injury. Additionally, enhanced effectiveness elevating MUC2 (0.53 ± 0.07), improving mucus integrity, reducing permeability Moreover, increased proportion beneficial bacteria Firmicutes (33.01 0.09%) downregulated harmful Proteobacteria (0.32 0.27%). These suggest possess ability regulate immune responses, protect barriers, gut microbiota. research presents intervention approach prevent

Language: Английский

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Bacterial Outer Membrane Vesicles and Immune Modulation of the Host

Membranes, Journal Year: 2023, Volume and Issue: 13(9), P. 752 - 752

Published: Aug. 24, 2023

This article reviews the role of outer membrane vesicles (OMVs) in mediating interaction between Gram-negative bacteria and their human hosts. OMVs are produced by a diverse range during infection play critical facilitating host-pathogen interactions without requiring direct cell-to-cell contact. describes mechanisms which formed subsequently interact with host cells, leading to transport microbial protein virulence factors short interfering RNAs (sRNA) targets, exerting immunomodulatory effects targeting specific signaling pathways. Specifically, this review highlights facilitate chronic through epigenetic modification immune response. Finally, identifies knowledge gaps field offers potential avenues for future OMV research, specifically regarding rigor reproducibility isolation characterization methods.

Language: Английский

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Recent progress on engineered micro/nanomaterials mediated modulation of gut microbiota for treating inflammatory bowel disease

Journal of Controlled Release, Journal Year: 2024, Volume and Issue: 370, P. 43 - 65

Published: April 17, 2024

Language: Английский

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Extracellular Vesicles from Nanomedicine‐Trained Intestinal Microbiota Substitute for Fecal Microbiota Transplant in Treating Ulcerative Colitis

Advanced Materials, Journal Year: 2024, Volume and Issue: 36(41)

Published: July 29, 2024

Abstract The biosafety concerns associated with fecal microbiota transplant (FMT) limit their clinical application in treating ulcerative colitis (UC). Gut secrete abundant extracellular vesicles (Gm‐EVs), which play a critical role bacteria‐to‐bacteria and bacteria‐to‐host communications. Herein, intestinal are trained using tea leaf lipid/pluronic F127‐coated curcumin nanocrystals (CN@Lp 127 s), can maintain stability during transit through the gastrointestinal tract. Compared FMT, Gm‐EVs derived from healthy mice significantly improve treatment outcomes against UC by reducing colonic inflammatory responses, restoring barrier function, rebalancing microbiota. Strikingly, obtained CN@Lp ‐trained exhibit superior therapeutic effect on compared to groups receiving FMT mice, mice. Oral administration of not only alleviates inflammation, promotes mucosal repair, regulates gut but also purine metabolism decrease uric acid level, resulting robust improvement UC. This study demonstrates efficacy nanomedicine‐trained regulating immune microenvironment, microbiota, colon. These EVs provide an alternative platform replace as for

Language: Английский

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