Near-Infrared Optogenetic Nanosystem for Spatiotemporal Control of CRISPR-Cas9 Gene Editing and Synergistic Photodynamic Therapy DOI
Junyi Zeng, Xinbo Huang,

Yajie Yang

et al.

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 17(1), P. 701 - 710

Published: Dec. 16, 2024

Controlling CRISPR/Cas9 gene editing at the spatiotemporal resolution level, especially for in vivo applications, remains a great challenge. Here, we developed near-infrared (NIR) light-activated nanophotonic system (UCPP) controlled CRISPR-Cas9 and synergistic photodynamic therapy (PDT). Lanthanide-doped upconversion nanoparticles are not only employed as carriers intracellular plasmid delivery but also serve nanotransducers to convert NIR light (980 nm) into visible with emission 460 650 nm, which could result simultaneous activation of PDT processes, respectively. Such unique design achieves light-controlled precise hypoxia-inducible factor 1α minimal off-target effect, effectively ameliorates hypoxic state tumor sites, facilitates deep-seated process antitumor effect. This optogenetically activatable nanosystem holds potential spatially targeted cancer therapy.

Language: Английский

Innovative Applications of Bacteria and Their Derivatives in Targeted Tumor Therapy DOI

Denghui Zhu,

Wei Pan,

Heqi Li

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 28, 2025

Despite significant progress in cancer treatment, traditional therapies still face considerable challenges, including poor targeting, severe toxic side effects, and the development of resistance. Recent advances biotechnology have revealed potential bacteria their derivatives as drug delivery systems for tumor therapy by leveraging biological properties. Engineered bacteria, Escherichia coli, Salmonella, Listeria monocytogenes, along with derivatives─outer membrane vesicles (OMVs), bacterial ghosts (BGs), spores (BSPs)─can be loaded a variety antitumor agents, enabling precise targeting sustained release within microenvironment (TME). These possess intrinsic properties that stimulate immune system, enhancing both innate adaptive responses to further amplify therapeutic effects. The ability naturally accumulate hypoxic regions versatility genetic modifications allow tailored strategies synergistically enhance effectiveness chemotherapy, immunotherapy, targeted therapies. This review comprehensively examines fundamental principles therapy, focusing on employed engineering, loading, use therapy. It also discusses challenges faced optimizing systems, such safety concerns, unintended responses, scalability clinical applications. By exploring these aspects, this provides theoretical framework improving bacterial-based contributing more effective personalized treatments.

Language: Английский

Citations

3
Surface Engineering on Bacteria for Tumor Immunotherapy: Strategies and Perspectives DOI
Lijun Fu, Qing‐Yu He, Xiaoyang Lu

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: May 17, 2024

Abstract Bacteria have garnered significant attention in tumor immunotherapy recently years, owing to their immune‐activating properties, ease of genetic manipulation, and colonization abilities. In order further enhance the safety, efficacy, clinical translation potential bacterial therapy, endow bacteria with additional functions, modifications or engineering surfaces emerged as a current research hotspot. This review systematically summarizes primary methods strategies for surface modification engineering, including chemical alteration, physical modification, bio‐modification. Subsequently, it is delve into roles these techniques enhancing immunotherapy, such improving immunotherapeutic efficacy reducing toxic side effects, elucidate underlying mechanisms. Finally, challenges faced this field are deeply explored, solutions future presented. work offers comprehensive overview advancements new generation implications bacterial‐based immunotherapy.

Language: Английский

Citations

14
A hypoxia-activated and microenvironment-remodeling nanoplatform for multifunctional imaging and potentiated immunotherapy of cancer DOI Creative Commons
Jianwen Song, He Wang, Xue Meng

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Nov. 29, 2024

Language: Английский

Citations

10
Nanosonosensitizer Optimization for Enhanced Sonodynamic Disease Treatment DOI
Min Zhang, Dandan Sun, Hui Huang

et al.

Advanced Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 23, 2024

Low-intensity ultrasound-mediated sonodynamic therapy (SDT), which, by design, integrates sonosensitizers and molecular oxygen to generate therapeutic substances (e.g., toxic hydroxyl radicals, superoxide anions, or singlet oxygen) at disease sites, has shown enormous potential for the effective treatment of a variety diseases. Nanoscale play crucial role in SDT process because their structural, compositional, physicochemical, biological characteristics are key determinants efficacy. In particular, advances materials science nanotechnology have invigorated series optimization strategies augmenting efficacy nanosonosensitizers. This comprehensive review systematically summarizes, discusses, highlights state-of-the-art studies on current achievements nanosonosensitizer enhanced treatment, with an emphasis general design principles nanosonosensitizers strategies, mainly including organic inorganic Additionally, recent advancements optimized applications aimed treating various diseases, such as cancer, bacterial infections, atherosclerosis, autoimmune clarified detail. Furthermore, effects improved versatile thoroughly discussed. The concludes highlighting challenges future opportunities this rapidly evolving research field expedite its practical clinical translation application.

Language: Английский

Citations

9
Targeting the Adenosine‐Mediated Metabolic Immune Checkpoint with Engineered Probiotic for Enhanced Chemo‐Immunotherapy DOI Creative Commons
Jinhui Wang, Jing Wang,

Zhijie Yu

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 22, 2025

Abstract Immunotherapy has revolutionized cancer treatment by leveraging the patient's immune system, yet its efficacy is often hampered immunosuppressive tumor microenvironment (TME). Adenosine, a key player in this milieu, suppresses cell activity via cAMP signaling. Here, an innovative strategy to remodel TME using genetically engineered strain of Escherichia coli Nissle 1917 that expresses adenosine deaminase on surface under hypoxic conditions presented. This probiotic targets tumors, converts inosine, and enhances anti‐tumor responses. In vivo, significantly improved infiltration demonstrated synergistic effects with low‐dose doxorubicin both subcutaneous orthotopic mouse colorectal model. Furthermore, modulated TME, promoting shift from M2‐like M1‐like macrophages increasing effector T populations. These findings highlight potential probiotics for metabolic modulation offering novel approach enhancing immunotherapy.

Language: Английский

Citations

1
Lactate/Cysteine Dual-Consuming Probiotic–Nanomedicine Biohybrid System for Enhanced Cancer Chemo-Immunotherapy DOI

Tian‐Qiu Xie,

Xiao Yan,

You‐Teng Qin

et al.

Nano Letters, Journal Year: 2024, Volume and Issue: 24(50), P. 16132 - 16142

Published: Dec. 6, 2024

Immunotherapy is revolutionizing oncology, but its therapeutic efficiency still limited by the off-target toxicities and poor antitumor immune responses. By integrating drug-loaded nanoparticles (DMnSH) with unique metabolic traits of Veillonella parvula (Vei), a probiotic–nanomedicine conjugate Vei@DMnSH biohybrid elaborately designed for enhanced cancer chemo-immunotherapy. Specifically, can accumulate in hypoxic tumor sites simultaneously consume lactate cysteine to reverse lactate-associated immunosuppression impede biosynthesis GSH. In addition, DMnSH will rapidly deplete intracellular GSH disassemble release DOX Mn2+. Accompanied two-pronged depletion, Mn2+-mediated Fenton-like reaction effectively generate oxidative hydroxyl radicals induce heavy redox imbalance. Combined effect DOX, robust immunogenic cell death provoked subsequently activates adaptive immunity suppression rate over 82%, synergistically enhancing outcomes

Language: Английский

Citations

5
NIR light-activated nanocomposites combat biofilm formation and enhance antibacterial efficacy for improved wound healing DOI Creative Commons

I. Ullah,

Shahin Shah Khan, Waqar Ahmad

et al.

Communications Chemistry, Journal Year: 2024, Volume and Issue: 7(1)

Published: June 8, 2024

Abstract Nanoparticle-based therapies are emerging as a pivotal frontier in biomedical research, showing their potential combating infections and facilitating wound recovery. Herein, selenium-tellurium dopped copper oxide nanoparticles (SeTe-CuO NPs) with dual photodynamic photothermal properties were synthesized, presenting an efficient strategy for bacterial infections. In vitro evaluations revealed robust antibacterial activity of SeTe-CuO NPs, achieving up to 99% eradication bacteria significant biofilm inhibition upon near-infrared (NIR) irradiation. Moreover, vivo studies demonstrated accelerated closure treatment NIR-activated demonstrating efficacy promoting healing. Furthermore, NPs exhibited rapid clearance within wounds, offering promising solution care. Overall, this versatile platform holds great promise multidrug-resistant advancing therapeutic interventions management.

Language: Английский

Citations

4
Genetically Designed Living Bacteria with Melanogenesis for Tumor‐Specific Pigmentation and Therapeutic Intervention DOI Creative Commons
Liying Wang, Qi Wu,

Qi Lyu

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 11(31)

Published: June 18, 2024

Abstract Visual observation and therapeutic intervention against tumors hold significant appeal for tumor treatment, particularly in meeting the demands of intraoperative navigation. From a clinical perspective, naked‐eye visualization provides direct convenient approach to identifying navigating during surgery. Nevertheless, there is an ongoing need develop effective solutions this frontier. Genetically engineered microorganisms are promising as living therapeutics combatting malignant tumors, leveraging precise targeting versatile programmed functionalities. Here, genetically modified Escherichia coli ( E. ) MG1655 bacterial cells introduced, called MelaBac cells, designed express tyrosinase continuously. This bioengineered melanogenesis produces melanin capable pigmenting both subcutaneous CT26 xenografts chemically induced colorectal cancer (CRC). Additionally, demonstrate initiation photonic hyperthermia therapy immunotherapy offering selective interventions with high biocompatibility.

Language: Английский

Citations

4
Nanomedicine hitchhiking on bacteria for treating tumors DOI Creative Commons

Shujing Zheng,

Xingwei Li,

Shutao Guo

et al.

BMEMat, Journal Year: 2024, Volume and Issue: unknown

Published: June 25, 2024

Abstract Nanomedicine has shown promising therapeutic potential in cancer treatment, with clinically approved formulations such as Doxil® and Abraxane® already providing tangible benefits to patients. However, challenges low targeting efficiency poor tumor penetration limit its application. Bacteria have emerged drug delivery carriers due their capacity for autonomous navigation deep into hypoxic parenchyma. Therefore, utilizing bacteria nanomedicine can partially overcome the limitations of anti‐tumor nanomedicine. Moreover, some bacteria, like Salmonella typhimurium Escherichia coli , exhibit immunostimulatory oncolytic effects synergistically enhance This article summarizes common types nanomedicines respective advantages treatment. It elaborates on various strategies combining under different administration routes, outlining clinical progress bacterial therapy outlooking future applications

Language: Английский

Citations

4
Advances in Engineered Bacteria for Cancer Therapy DOI Creative Commons
Wenping Pan, Hongmei Liu, Decheng Wu

et al.

Precision medicine and engineering., Journal Year: 2025, Volume and Issue: unknown, P. 100017 - 100017

Published: Feb. 1, 2025

Language: Английский

Citations

0