Probing the Complexities of Astrocyte Calcium Signaling

Trends in Cell Biology, Journal Year: 2016, Volume and Issue: 26(4), P. 300 - 312

Published: Feb. 19, 2016

Language: Английский

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Zebrafish Behavior: Opportunities and Challenges

Annual Review of Neuroscience, Journal Year: 2017, Volume and Issue: 40(1), P. 125 - 147

Published: April 4, 2017

A great challenge in neuroscience is understanding how activity the brain gives rise to behavior. The zebrafish an ideal vertebrate model address this challenge, thanks capacity, at larval stage, for precise behavioral measurements, genetic manipulations, and recording manipulation of neural noninvasively single-neuron resolution throughout whole brain. These techniques are being further developed application freely moving animals juvenile stages study more complex behaviors including learning, decision making, social interactions. We review some approaches that have been used behavior point opportunities challenges lie ahead.

Language: Английский

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Advancements in zebrafish applications for 21st century toxicology

Pharmacology & Therapeutics, Journal Year: 2016, Volume and Issue: 161, P. 11 - 21

Published: March 22, 2016

Language: Английский

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Rational Engineering of XCaMPs, a Multicolor GECI Suite for In Vivo Imaging of Complex Brain Circuit Dynamics

Cell, Journal Year: 2019, Volume and Issue: 177(5), P. 1346 - 1360.e24

Published: May 1, 2019

Language: Английский

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Dynamic labelling of neural connections in multiple colours by trans-synaptic fluorescence complementation

Nature Communications, Journal Year: 2015, Volume and Issue: 6(1)

Published: Dec. 4, 2015

Abstract Determining the pattern of activity individual connections within a neural circuit could provide insights into computational processes that underlie brain function. Here, we develop new strategies to label active synapses by trans-synaptic fluorescence complementation in Drosophila . First, demonstrate synaptobrevin-GRASP chimera functions as powerful activity-dependent marker for vivo Next, create cyan and yellow variants, achieving activity-dependent, multi-colour reconstitution across (X-RASP). Our system allows first time retrospective labelling (rather than whole neurons) based on their activity, multiple colours, same animal. As often act units brain, our method will promote design experiments are not possible using existing techniques. Moreover, easily adaptable mapping any genetic system.

Language: Английский

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Pan-neuronal calcium imaging with cellular resolution in freely swimming zebrafish

Nature Methods, Journal Year: 2017, Volume and Issue: 14(11), P. 1107 - 1114

Published: Sept. 11, 2017

Language: Английский

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Mapping the Neural Substrates of Behavior

Cell, Journal Year: 2017, Volume and Issue: 170(2), P. 393 - 406.e28

Published: July 1, 2017

Language: Английский

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Enteroendocrine cells sense bacterial tryptophan catabolites to activate enteric and vagal neuronal pathways

Cell Host & Microbe, Journal Year: 2020, Volume and Issue: 29(2), P. 179 - 196.e9

Published: Dec. 21, 2020

Language: Английский

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On Myelinated Axon Plasticity and Neuronal Circuit Formation and Function

Journal of Neuroscience, Journal Year: 2017, Volume and Issue: 37(42), P. 10023 - 10034

Published: Oct. 18, 2017

Studies of activity-driven nervous system plasticity have primarily focused on the gray matter. However, MRI-based imaging studies shown that white matter, composed myelinated axons, can also be dynamically regulated by activity healthy brain. Myelination in CNS is an ongoing process starts around birth and continues throughout life. Myelin generated oligodendrocytes recent evidence has many aspects oligodendrocyte development myelination modulated extrinsic signals including neuronal activity. Because modulation myelin can, turn, affect several conduction, concept emerged activity-regulated represents important form plasticity. Here we review our increasing understanding how regulates axons vivo , with a focus timing relevant processes. We highlight observations rapidly tune axonal diameter, promote re-entry progenitor cells into cell cycle, or drive their direct differentiation oligodendrocytes. suggest formation remodeling significantly change conduction properties are most likely to occur over timescales days weeks. Finally, propose precise fine-tuning along already-myelinated may mediated alterations axon itself. conclude future need analyze adaptations both sheaths fully understand contributes circuit function.

Language: Английский

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A light- and calcium-gated transcription factor for imaging and manipulating activated neurons

Nature Biotechnology, Journal Year: 2017, Volume and Issue: 35(9), P. 864 - 871

Published: June 26, 2017

Language: Английский

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