Scientific Reports,
Journal Year:
2020,
Volume and Issue:
10(1)
Published: June 25, 2020
Abstract
Exposure
to
stress
during
early
life
affects
subsequent
behaviors
and
increases
the
vulnerability
adult
pathologies,
a
phenomenon
that
has
been
well
documented
in
humans
rodents.
In
this
study,
we
introduce
chronic
unpredictable
protocol
adapted
young
zebrafish,
which
is
an
increasingly
popular
vertebrate
model
neuroscience
research.
We
exposed
zebrafish
series
of
intermittent
mild
stressors
from
day
10
17
post-fertilization.
The
stressed
fish
showed
reduced
exploration
novel
environment
one
post-stress
increased
responsiveness
dark-light
transition
two
days
post-stress,
indicative
heightened
anxiety-related
behaviors.
stress-induced
decrease
lasted
for
at
least
three
returned
control
levels
within
week.
Moreover,
were
on
average
8%
smaller
than
their
siblings
All
together,
our
results
demonstrate
develop
growth
behavioral
alterations
akin
those
observed
rodent
models.
Biomedicines,
Journal Year:
2019,
Volume and Issue:
7(1), P. 23 - 23
Published: March 26, 2019
Zebrafish
larvae
show
a
clear
and
distinct
pattern
of
swimming
in
response
to
light
dark
conditions,
following
the
development
swim
bladder
at
4
days
post
fertilization.
This
behavior
is
increasingly
employed
screening
neuroactive
drugs.
The
recent
emergence
high-throughput
techniques
for
automatic
tracking
zebrafish
has
further
allowed
an
objective
efficient
way
finding
subtle
behavioral
changes
that
could
go
unnoticed
during
manual
observations.
review
highlights
use
as
model
compounds.
We
describe,
brief,
repertoire
larvae.
Then,
we
focus
on
utilization
light-dark
locomotion
test
identifying
BMC Biology,
Journal Year:
2018,
Volume and Issue:
16(1)
Published: Feb. 23, 2018
The
need
for
high-throughput,
precise,
and
meaningful
methods
measuring
behavior
has
been
amplified
by
our
recent
successes
in
manipulating
neural
circuitry.
largest
challenges
associated
with
moving
this
direction,
however,
are
not
technical
but
instead
conceptual:
what
numbers
should
one
put
on
the
movements
an
animal
is
performing
(or
performing)?
In
review,
I
will
describe
how
theoretical
data
analytical
ideas
interfacing
recently-developed
computational
experimental
methodologies
to
answer
these
questions
across
a
variety
of
contexts,
length
scales,
time
scales.
attempt
highlight
commonalities
between
approaches
areas
where
further
advances
necessary
place
same
quantitative
footing
as
other
scientific
fields.
Frontiers in Science,
Journal Year:
2023,
Volume and Issue:
1
Published: Feb. 28, 2023
Recent
advances
in
human
stem
cell-derived
brain
organoids
promise
to
replicate
critical
molecular
and
cellular
aspects
of
learning
memory
possibly
cognition
vitro
.
Coining
the
term
“organoid
intelligence”
(OI)
encompass
these
developments,
we
present
a
collaborative
program
implement
vision
multidisciplinary
field
OI.
This
aims
establish
OI
as
form
genuine
biological
computing
that
harnesses
using
scientific
bioengineering
an
ethically
responsible
manner.
Standardized,
3D,
myelinated
can
now
be
produced
with
high
cell
density
enriched
levels
glial
cells
gene
expression
for
learning.
Integrated
microfluidic
perfusion
systems
support
scalable
durable
culturing,
spatiotemporal
chemical
signaling.
Novel
3D
microelectrode
arrays
permit
high-resolution
electrophysiological
signaling
recording
explore
capacity
recapitulate
mechanisms
formation
and,
ultimately,
their
computational
potential.
Technologies
could
enable
novel
biocomputing
models
via
stimulus-response
training
organoid-computer
interfaces
are
development.
We
envisage
complex,
networked
whereby
connected
real-world
sensors
output
devices,
ultimately
each
other
sensory
organ
(e.g.
retinal
organoids),
trained
biofeedback,
big-data
warehousing,
machine
methods.
In
parallel,
emphasize
embedded
ethics
approach
analyze
ethical
raised
by
research
iterative,
manner
involving
all
relevant
stakeholders.
The
many
possible
applications
this
urge
strategic
development
discipline.
anticipate
OI-based
allow
faster
decision-making,
continuous
during
tasks,
greater
energy
data
efficiency.
Furthermore,
“intelligence-in-a-dish”
help
elucidate
pathophysiology
devastating
developmental
degenerative
diseases
(such
dementia),
potentially
aiding
identification
therapeutic
approaches
address
major
global
unmet
needs.
International Journal of Molecular Sciences,
Journal Year:
2022,
Volume and Issue:
23(12), P. 6647 - 6647
Published: June 14, 2022
To
discover
new
molecules
or
review
the
biological
activity
and
toxicity
of
therapeutic
substances,
drug
development,
research
relies
on
robust
systems
to
obtain
reliable
results.
Phenotype-based
screenings
can
transpose
organism’s
compensatory
pathways
by
adopting
multi-target
strategies
for
treating
complex
diseases,
zebrafish
emerged
as
an
important
model
biomedical
screenings.
Zebrafish’s
clear
correlation
between
neuro-anatomical
physiological
features
behavior
is
very
similar
that
verified
in
mammals,
enabling
construction
relevant
experimental
models
neurological
disorders
research.
Zebrafish
presents
highly
conserved
are
found
higher
vertebrates,
including
along
with
a
behavioral
repertoire.
Moreover,
it
sensitive
pharmacological/environmental
manipulations,
these
phenotypes
detected
both
larvae
adults.
These
advantages
align
3Rs
concept
qualify
powerful
tool
pre-clinical
trials.
This
highlights
domains
studied
their
neurotransmitter
summarizes
currently
used
techniques
evaluate
quantify
laboratory
studies.
