Context-specific Polycomb mechanisms in development

Nature Reviews Genetics, Journal Year: 2022, Volume and Issue: 23(11), P. 680 - 695

Published: June 9, 2022

Language: Английский

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Dynamic chromatin regulatory programs during embryogenesis of hexaploid wheat

Genome biology, Journal Year: 2023, Volume and Issue: 24(1)

Published: Jan. 13, 2023

Abstract Background Plant and animal embryogenesis have conserved distinct features. Cell fate transitions occur during in both plants animals. The epigenomic processes regulating plant remain largely elusive. Results Here, we elucidate chromatin transcriptomic dynamics of the most cultivated crop, hexaploid wheat. Time-series analysis reveals stage-specific proximal–distal accessibility concordant with transcriptome changes. Following fertilization, remodeling kinetics H3K4me3, H3K27ac, H3K27me3 differ from that mammals, highlighting considerable species-specific zygotic genome activation. Polycomb repressive complex 2 (PRC2)-mediated deposition is important for embryo establishment. Later H3K27me3, undergo dramatic to establish a permissive environment facilitating access transcription factors cis -elements patterning. Embryonic maturation characterized by increasing decreasing accessibility, which likely participates restricting totipotency while preventing extensive organogenesis. Finally, signatures are correlated biased expression among homeolog triads divergent after polyploidization, revealing an contributor subgenome diversification allohexaploid genome. Conclusions Collectively, present invaluable resource comparative mechanistic regulation crop embryogenesis.

Language: Английский

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Epigenetic regulation of early human embryo development

Cell stem cell, Journal Year: 2023, Volume and Issue: 30(12), P. 1569 - 1584

Published: Oct. 18, 2023

Studies of mammalian development have advanced our understanding the genetic, epigenetic, and cellular processes that orchestrate embryogenesis uncovered new insights into unique aspects human embryogenesis. Recent studies now produced first epigenetic maps early embryogenesis, stimulating ideas about reprogramming, cell fate control, potential mechanisms underpinning developmental plasticity in embryos. In this review, we discuss these regulation importance for safeguarding development. We also highlight unanswered questions key challenges remain to be addressed.

Language: Английский

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Remodeling of maternal mRNA through poly(A) tail orchestrates human oocyte-to-embryo transition

Nature Structural & Molecular Biology, Journal Year: 2023, Volume and Issue: 30(2), P. 200 - 215

Published: Jan. 16, 2023

Abstract Poly(A)-tail-mediated post-transcriptional regulation of maternal mRNAs is vital in the oocyte-to-embryo transition (OET). Nothing known about poly(A) tail dynamics during human OET. Here, we show that length and internal non-A residues are highly dynamic OET, using poly(A)-inclusive RNA isoform sequencing (PAIso-seq). Unexpectedly, undergo global remodeling: after deadenylation or partial degradation into 3ʹ-UTRs, they re-polyadenylated to produce polyadenylated intermediates, coinciding with massive incorporation residues, particularly long consecutive U newly synthesized tails. Moreover, TUT4 TUT7 contribute these BTG4-mediated produces substrates for mRNA re-polyadenylation, TENT4A TENT4B incorporate G residues. The remodeling further confirmed PAIso-seq2. Importantly, essential first cleavage embryos. Together, findings broaden our understanding

Language: Английский

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Capturing totipotency in human cells through spliceosomal repression

Cell, Journal Year: 2024, Volume and Issue: 187(13), P. 3284 - 3302.e23

Published: June 1, 2024

Language: Английский

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Comparative proteomic landscapes elucidate human preimplantation development and failure

Cell, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

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Polycomb Group Proteins Regulate Chromatin Architecture in Mouse Oocytes and Early Embryos

Molecular Cell, Journal Year: 2019, Volume and Issue: 77(4), P. 825 - 839.e7

Published: Dec. 12, 2019

Language: Английский

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Allelic H3K27me3 to allelic DNA methylation switch maintains noncanonical imprinting in extraembryonic cells

Science Advances, Journal Year: 2019, Volume and Issue: 5(12)

Published: Dec. 6, 2019

Maternal H3K27me3–mediated imprinting switches to allelic DNA methylation maintain imprinted expression in extraembryonic cells.

Language: Английский

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Decoding dynamic epigenetic landscapes in human oocytes using single-cell multi-omics sequencing

Cell stem cell, Journal Year: 2021, Volume and Issue: 28(9), P. 1641 - 1656.e7

Published: May 5, 2021

Language: Английский

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The role and mechanisms of DNA methylation in the oocyte

Essays in Biochemistry, Journal Year: 2019, Volume and Issue: 63(6), P. 691 - 705

Published: Nov. 29, 2019

Abstract Epigenetic information in the mammalian oocyte has potential to be transmitted next generation and influence gene expression; this occurs naturally case of imprinted genes. Therefore, it is important understand how epigenetic patterned during development growth. Here, we review current state knowledge de novo DNA methylation mechanisms oocyte: a distinctive gene-body pattern created, extent which machinery reads chromatin states. Recent epigenomic studies building on advances ultra-low input profiling methods, coupled with genetic studies, have started allow detailed interrogation interplay between establishment states; however, full mechanistic description awaits.

Language: Английский

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