Engineered Living Systems Based on Gelatin: Design, Manufacturing, and Applications

Advanced Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 5, 2025

Engineered living systems (ELSs) represent purpose-driven assemblies of components, encompassing cells, biomaterials, and active agents, intricately designed to fulfill diverse biomedical applications. Gelatin its derivatives have been used extensively in ELSs owing their mature translational pathways, favorable biological properties, adjustable physicochemical characteristics. This review explores the intersection gelatin with fabrication techniques, offering a comprehensive examination synergistic potential creating for various applications biomedicine. It offers deep dive into gelatin, including structures production, sources, processing, properties. Additionally, techniques employing derivatives, generic microfluidics, 3D printing methods. Furthermore, it discusses based on regenerative engineering as well cell therapies, bioadhesives, biorobots, biosensors. Future directions challenges are also examined, highlighting emerging trends areas improvements innovations. In summary, this underscores significance gelatin-based advancing lays groundwork guiding future research developments within field.

Language: Английский

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Bioactive Decellularized Extracellular Matrix Platform Integrating Multifunctional Nanozymes and Cell-Laden Microgels for Acute Liver Failure Treatment

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 14, 2025

Mesenchymal stem cell (MSC) therapy has emerged as a promising alternative approach for treating acute liver failure (ALF) while confronting the shortage of low efficiency and poor engraftment within hostile milieu. In this study, we establish bioactive decellularized extracellular matrix (dECM) platform that incorporates dihydrolipoic acid (DHLA)-protected Pt nanoclusters doped with Cu (PtCu-DHLA) nanozymes cell-laden microgels. The PtCu-DHLA nanozymes, selected their versatility, function antioxidant, anti-inflammatory, pro-proliferative, pro-angiogenic agents, enhancing ALF alleviation providing an optimal microenvironment MSC transplantation. Additionally, methacrylic anhydride (MA)-modified porcine liver-derived (PLdECM) hydrogel (PLdECMMA) been developed construction microgels via microfluidic devices. Interferon γ (IFNγ) preconditioned MSCs encapsulated in PLdECMMA exhibit enhanced immunomodulating activity prolonged survival. are codelivered by leveraging PLdECM orthotopic transplanted dECM enables efficient successful rescue CCl4-induced counteracting oxidative stress, suppressing inflammatory storms, promoting cellular regeneration. Overall, study highlights synergistic reinforced strategy combines biomimetic therapy, offering significant potential treatment broader applications regenerative medicine.

Language: Английский

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3D bioprinted dynamic bioactive living construct enhances mechanotransduction-assisted rapid neural network self-organization for spinal cord injury repair

Bioactive Materials, Journal Year: 2025, Volume and Issue: 46, P. 531 - 554

Published: Jan. 8, 2025

Language: Английский

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A review of 3D bioprinting for organoids

Medical Review, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 13, 2025

Abstract Current two-dimensional (2D) cell models for effective drug screening suffer from significant limitations imposed by the lack of realism in physiological environment. Three-dimensional (3D) organoids hold immense potential mimicking key functions human organs overcoming traditional 2D models. However, current techniques preparation 3D had reproducibility, scalability, and ability to closely replicate complex microenvironment found vivo . Additionally, culture systems often involve lengthy labor-intensive processes that hinder high-throughput applications necessary a large-scale screening. Advancements bioprinting technologies offer promising solutions these challenges enabling precise spatial control over placement material composition, thereby facilitating creation more physiologically relevant than techniques. This review provides comprehensive summary recent advances creating models, which begins with an introduction different types (especially focus on volumetric (VBP) technique), followed overview bioinks utilized bioprinting. Moreover, we also introduce disease efficiency evaluation regenerative medicine. Finally, possible strategies development clinical translation are concluded.

Language: Английский

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Emergent biotechnology applications in urology: a mini review

Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 13

Published: Feb. 4, 2025

Technological advances have significantly impacted the field of urology, providing innovative solutions for diagnosis, treatment, and management various urological disorders diseases. This article highlights four groundbreaking technologies: whole-cell biosensors, optogenetic interventions neuromodulation, bioengineered urinary bladder, 3D bioprinting. Each technology plays a crucial role in enhancing patient care improving clinical outcomes urology. Advances these fields underscore shift towards precision diagnostics, personalized treatments, enhanced regenerative strategies, ultimately aiming to enhance address unmet needs

Language: Английский

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Bioinspired Paste‐Extrusion Printed Microlattices with Natural Bone‐Like Porosity and Performance

Small, Journal Year: 2025, Volume and Issue: unknown

Published: March 10, 2025

The structure feature determines its performance. In the field of biological implants, microlattices are commonly used as building blocks for light-weight and adaptive purposes, which however show limitations in mechanical properties compared with natural bones. Inspired by efficient mass transfer high fault tolerance neural networks derived from hierarchical functional gradient, a bioinspired paste-extrusion printed microlattice (BPPM) is developed tunable demonstrated. non-crossing structures first verified outweigh crossing one under equivalent compressive stress. Then, introducing gradient components 3D printing process, BPPM porosity, composites fabricated. As result, shows eliminated deformation along direction, fine surface roughness (Sa 3.65-15.67 µm), wide range porosity (56-78%) strength (3.44-22.3 MPa), favorable permeability (3.02 × 103-3.22 103D), good biocompatibility promoted cell proliferation. This work not only demonstrates bones but also provides robust way to realize it.

Language: Английский

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The road ahead in materials and technologies for volumetric 3D printing

Nature Reviews Materials, Journal Year: 2025, Volume and Issue: unknown

Published: March 18, 2025

Language: Английский

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Imaging‐Guided Microscale Photothermal Stereolithography Bioprinting

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: March 20, 2025

Stereolithography bioprinting relies heavily on costly photoinitiators for polymerization, limiting its potential further technical advancement to meet growing needs in tissue engineering and regenerative medicine. Thermal initiators, contrast, are low cost, rapid growth of the photothermal conversion field offers a wide range materials tools convert light into heat. However, high-resolution stereolithography remains unattainable due difficulty confining heat an aqueous environment. Here, this challenge has been fully addressed by establishing imaging-guided microscale (ImPSB). This technique is achieved through building novel system that provides depth-resolved visualization printing dynamics, creating unique initiator second near-infrared window, developing new bioink seeing controlling gelation process. ImPSB achieves resolution ≈47 µm generates smooth lines arbitrarily designed shapes with cross-sectional diameter as small ≈104 µm, representing unprecedented scale from stereolithography. Its cellular biocompatibility both bioscaffold cell-laden hydrogel demonstrated, feasibility transdermal also shown. work sets path where vast resources can be utilized.

Language: Английский

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Revolutionizing Bone Repair and Regeneration: The Role of Machine Learning in Designing Advanced Nanocomposite Hydrogels

Polymers for Advanced Technologies, Journal Year: 2025, Volume and Issue: 36(4)

Published: March 28, 2025

ABSTRACT Bone repair is a complex biological process requiring dynamic interplay between cellular mechanisms, molecular signaling, and environmental factors. The intricate stages of bone healing, including hematoma formation, inflammation, soft callus development, hard remodeling, are driven by coordinated responses pathways. Proinflammatory cytokines, growth factors, the extracellular matrix play critical roles in promoting osteogenesis angiogenesis. Factors such as age, systemic health, mechanical stability significantly influence efficiency. To address limitations natural advancements regenerative medicine have introduced innovative materials like nanocomposite hydrogels, which mimic microenvironment enhance function. Semi‐interpenetrating network (semi‐IPN) hydrogels emerged promising tool for tissue engineering. Combining crosslinked non‐crosslinked polymers, these offer balance stability, functionality, controlled degradation. Semi‐IPN provide structural support, facilitate cell attachment, enable sustained release bioactive molecules. Their flexibility adaptability make them suitable encapsulating stem cells targeted regeneration. Moreover, nonsurgical surgical scaffold delivery methods, ranging from injectable to 3D‐printed magnetically guided scaffolds, expanded horizons strategies, reduced invasiveness, improved patient outcomes. This review explores dynamics role regeneration, advanced construction strategies semi‐IPN repair. By integrating polymer science, nanotechnology, bioengineering, represent transformative shift addressing defects, paving way therapeutic approaches medicine. With ongoing advancements, technologies hold significant potential improve effectiveness accessibility solutions.

Language: Английский

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A Microgel–Hydrogel Hybrid for Functional Compensation and Mechanical Stability in 3D Printed Cell‐Dense Vascularized Liver Tissue

Advanced Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 13, 2025

Abstract 3D bioprinting of liver tissue with high cell density (HCD) shows great promise for restoring function in cases acute failure, where a substantial number functional cells are required to perform essential physiological tasks. Direct vascular anastomosis is critical the successful implantation these bioprinted vascularized tissues into host vasculature, allowing rapid compensation and addressing various conditions. However, conventional hydrogels used encapsulate high‐density often lack mechanical properties needed withstand shear forces blood flow, resulting failure. In this study, heterogeneous microgel–hydrogel hybrid developed carry HCD hepatocytes support embedded hierarchical structures. By optimizing ratio microgel biomacromolecule, covalently crosslinked network offers integrity enables direct anastomosis, ensuring efficient nutrient oxygen exchange. The thick, constructs, containing hepatocytes, successfully implanted rats after 85% hepatectomy, leading swift recovery prolonged survival. This study presents strategy enhance regenerative therapy outcomes through advanced integration techniques.

Language: Английский

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