Functional optic tract rewiring via subtype- and target-specific axonal regeneration and presynaptic activity enhancement DOI Creative Commons
Xin Zhang, Chao Yang, Chengle Zhang

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: March 4, 2025

Mechanisms underlying functional axonal rewiring after adult mammalian central nervous system (CNS) injuries remain unclear partially due to limited models. Here we develop a mouse intracranial pre-olivary pretectal nucleus (OPN) optic tract injury model and demonstrate that Pten/Socs3 knockout CNTF expression in retinal ganglion cells (RGCs) promotes regeneration OPN reinnervation. Revealed by transmission electron microscopy, trans-synaptic labeling, electrophysiology, synapses are formed mainly intrinsically photosensitive RGCs, thereby restoring the pupillary light reflex (PLR). Moreover, combining with Lipin1 knockdown accelerates recovery achieves reconnection chronic injury. PLR can be further boosted increasing RGC photosensitivity melanopsin overexpression, it also enhanced treatment of voltage-gated calcium channel modulator augment presynaptic release. These findings highlight importance neuronal types activity for CNS injuries.

Language: Английский

Single-cell and spatial atlases of spinal cord injury in the Tabulae Paralytica DOI
Michael A. Skinnider, Matthieu Gautier, Yue Yang Teo

et al.

Nature, Journal Year: 2024, Volume and Issue: 631(8019), P. 150 - 163

Published: June 19, 2024

Language: Английский

Citations

26
Integrating hydrogels manipulate ECM deposition after spinal cord injury for specific neural reconnections via neuronal relays DOI Creative Commons
Zan Tan, Longyou Xiao, Junwu Ma

et al.

Science Advances, Journal Year: 2024, Volume and Issue: 10(27)

Published: July 3, 2024

A bioinspired hydrogel composed of hyaluronic acid-

Language: Английский

Citations

20
Neuronal maturation and axon regeneration: unfixing circuitry to enable repair DOI
Brett J. Hilton, Jarred M. Griffin, James W. Fawcett

et al.

Nature reviews. Neuroscience, Journal Year: 2024, Volume and Issue: 25(10), P. 649 - 667

Published: Aug. 20, 2024

Language: Английский

Citations

18
Caffeic acid phenethyl ester inhibits neuro-inflammation and oxidative stress following spinal cord injury by mitigating mitochondrial dysfunction via the SIRT1/PGC1α/DRP1 signaling pathway DOI Creative Commons
Yanan Zhang, Qian Deng,

Hongxiang Hong

et al.

Journal of Translational Medicine, Journal Year: 2024, Volume and Issue: 22(1)

Published: March 25, 2024

Abstract Background The treatment of spinal cord injury (SCI) has always been a significant research focus clinical neuroscience, with inhibition microglia-mediated neuro-inflammation as well oxidative stress key to successful SCI patient treatment. Caffeic acid phenethyl ester (CAPE), compound extracted from propolis, both anti-inflammatory and anti-oxidative effects, but its therapeutic effects have rarely reported. Methods We constructed mouse contusion model administered CAPE intraperitoneally for 7 consecutive days after injury, methylprednisolone (MP) was used positive control. Hematoxylin–eosin, Nissl, Luxol Fast Blue staining were assess the effect on structures nervous tissue SCI. Basso Mouse Scale scores footprint analysis explore recovery motor function by mice. Western blot immunofluorescence assessed levels inflammatory mediators stress-related proteins in vivo vitro Further, reactive oxygen species (ROS) within cytoplasm detected using an ROS kit. Changes mitochondrial membrane potential 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethyl-imidacarbocyanine iodide. Mechanistically, western examine SIRT1/PGC1α/DRP1 signaling pathway. Results CAPE-treated mice showed less neuronal loss, more survival, reduced demyelination. Interestingly, treated better function. expression mediators, including iNOS, COX-2, TNF-α, IL-1β, 1L-6, NOX-2, NOX-4, control MP vivo. In addition, molecular docking experiments that had high affinity SIRT1, significantly activated SIRT1 PGC1α, down-regulation DRP1. level cellular increased potential, which improved normal After administering inhibitor nicotinamide, reversed.On contrary, agonist SRT2183 further enhanced antioxidant CAPE, indicating dependent SIRT1. Conclusion inhibits supports regulating pathway These demonstrate reduces nerve damage. Therefore, is drug through production effects. Graphical

Language: Английский

Citations

14
The 2023 yearbook of Neurorestoratology DOI Creative Commons
Hongyun Huang, John R. Bach,

Hari Shanker Sharma

et al.

Journal of Neurorestoratology, Journal Year: 2024, Volume and Issue: 12(3), P. 100136 - 100136

Published: June 13, 2024

Remarkable advancements have been made in understanding the pathogenesis of Alzheimer's disease, Parkinson's and other neurological disease; our depth neurorestorative mechanisms such as anti-inflammatory processes, immune regulation, neuromodulation, neovascularization/neural repair, neuroprotection; clinical treatments. Multiple types cell therapies reported, with some positive outcomes. Diverse forms neurostimulation neuromodulation well brain–computer interfaces shown good therapeutic outcomes applications. Further, surgery pharmaceutic therapy very impressive. These fundamental achievements are helpful for diseases neurorestoration. Patients impairments benefited from progress, but these still require confirmation higher-level randomized trials.

Language: Английский

Citations

11
Graphene oxide scaffolds promote functional improvements mediated by scaffold-invading axons in thoracic transected rats DOI
Marta Zaforas, Esther Benayas, Raquel Madroñero-Mariscal

et al.

Bioactive Materials, Journal Year: 2025, Volume and Issue: 47, P. 32 - 50

Published: Jan. 10, 2025

Language: Английский

Citations

1
Low-cost and scalable projected light-sheet microscopy for the high-resolution imaging of cleared tissue and living samples DOI
Yannan Chen,

Shradha Chauhan,

Cheng Gong

et al.

Nature Biomedical Engineering, Journal Year: 2024, Volume and Issue: 8(9), P. 1109 - 1123

Published: Aug. 29, 2024

Language: Английский

Citations

8
Microenvironments‐Modulated Biomaterials Enhance Spinal Cord Injury Therapy DOI
Yuehong Li, Qingzheng Zhang, Zongtai Liu

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(46)

Published: Sept. 17, 2024

Abstract Spinal cord injury (SCI) results from various causes, including sports‐related incidents, degenerative cervical myelopathy, traffic accidents, and falls. SCI typically leads to sensory motor dysfunction even paralysis. Current treatments for include systemic administration of high‐dose steroids surgical decompression stabilization. However, excessive use glucocorticoids may increase susceptibility infections bleeding. The long‐term effect surgery intervention remains unclear, with ongoing debates regarding its timing, efficacy, safety. Therefore, innovative approaches are urgently needed alleviate secondary injuries promote spinal recovery. One emerging therapeutic approach is modulating the microenvironments achieve neuroprotection neurogenesis during Several biomaterials favorable physicochemical properties have been developed enhance effects by regulating microenvironments. This Review first discusses pathology then introduces biomaterials‐based regulatory strategies targeting microenvironmental components, anti‐inflammation, anti‐oxidation, reduction excitotoxicity, revascularization, neurogenesis, scar density reduction. Additionally, research clinical application prospects microenvironment regulation presented.

Language: Английский

Citations

8
Hypothalamic deep brain stimulation augments walking after spinal cord injury DOI
Newton Cho, Jordan W. Squair, Viviana Aureli

et al.

Nature Medicine, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 2, 2024

Language: Английский

Citations

8
Single-cell analysis of innate spinal cord regeneration identifies intersecting modes of neuronal repair DOI Creative Commons
Vishnu Muraleedharan Saraswathy, Zhou Li-li, Mayssa H. Mokalled

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Aug. 15, 2024

Adult zebrafish have an innate ability to recover from severe spinal cord injury. Here, we report a comprehensive single nuclear RNA sequencing atlas that spans 6 weeks of regeneration. We identify cooperative roles for adult neurogenesis and neuronal plasticity during repair. Neurogenesis glutamatergic GABAergic neurons restores the excitatory/inhibitory balance after In addition, transient population injury-responsive (iNeurons) show elevated 1 week post-injury. found iNeurons are injury-surviving acquire neuroblast-like gene expression signature CRISPR/Cas9 mutagenesis showed required functional recovery employ vesicular trafficking as essential mechanism underlies plasticity. This study provides resource cells mechanisms direct regeneration establishes model plasticity-driven neural

Language: Английский

Citations

6