Airway epithelium damage in acute respiratory distress syndrome DOI Creative Commons
Ludovic Gérard,

Marylène Lecocq,

Bruno Detry

et al.

Critical Care, Journal Year: 2024, Volume and Issue: 28(1)

Published: Oct. 30, 2024

The airway epithelium (AE) fulfils multiple functions to maintain pulmonary homeostasis, among which ensuring adequate barrier function, cell differentiation and polarization, actively transporting immunoglobulin A (IgA), the predominant mucosal in lumen, via polymeric receptor (pIgR). Morphological changes of airways have been reported ARDS, while their detailed features, impact for immunity, causative mechanisms remain unclear. Therefore, this study aimed assess epithelial alterations distal patients with ARDS.

Language: Английский

Cystic fibrosis DOI
Marcus Mall, Pierre–Régis Burgel, Carlo Castellani

et al.

Nature Reviews Disease Primers, Journal Year: 2024, Volume and Issue: 10(1)

Published: Aug. 8, 2024

Language: Английский

Citations

27
Bronchiectasis management in adults: state of the art and future directions DOI Creative Commons
Hayoung Choi, Pamela J. McShane, Stefano Aliberti

et al.

European Respiratory Journal, Journal Year: 2024, Volume and Issue: 63(6), P. 2400518 - 2400518

Published: May 23, 2024

Formerly regarded as a rare disease, bronchiectasis is increasingly recognised. A renewed interest in this disease has led to significant progress research. Randomised clinical trials (RCTs) have demonstrated the benefits of airway clearance techniques, inhaled antibiotics and long-term macrolide therapy patients. However, heterogeneity remains one most challenging aspects management. Phenotypes endotypes been identified help find “treatable traits” partially overcome complexity. The goals for are reduce symptom burden, improve quality life, exacerbations prevent progression. We review pharmacological non-pharmacological treatments that can mucociliary clearance, inflammation tackle infection, key pathophysiological features bronchiectasis. There also promising development management bronchiectasis, including novel anti-inflammatory therapies. This provides critical update on focusing treatable traits recent RCTs.

Language: Английский

Citations

17
Hypoxia-inducible factor 2 regulates alveolar regeneration after repetitive injury in three-dimensional cellular and in vivo models DOI
A. Scott McCall, Sergey Gutor,

Harikrisna Tanjore

et al.

Science Translational Medicine, Journal Year: 2025, Volume and Issue: 17(780)

Published: Jan. 8, 2025

Idiopathic pulmonary fibrosis (IPF) is a progressive interstitial lung disease in which repetitive epithelial injury and incomplete alveolar repair result accumulation of profibrotic intermediate/transitional “aberrant” cell states. The mechanisms leading to the emergence persistence aberrant populations distal remain incompletely understood. By interrogating single-cell RNA sequencing (scRNA-seq) data from patients with IPF mouse model repeated injury, we identified persistent activation hypoxia-inducible factor (HIF) signaling these cells. Using genetic lineage-tracing strategies together scRNA-seq, found that disease-emergent cells predominantly arose airway-derived ( Scgb1a1-CreER –traced) progenitors exhibited transcriptional programs Hif2a activation. In mice treated intratracheal bleomycin, deletion Epas1 (Hif2a) but not Hif1a , progenitors, or administration small-molecule HIF2 inhibitor PT-2385, using both prevention rescue approaches, attenuated experimental fibrosis, reduced appearance cells, promoted repair. organoids, pharmacologic inhibition Hif2 differentiation progenitors. addition, treatment human organoids PT-2385 increased colony-forming efficiency, enhanced protein markers type 2 maturation, prevented Together, studies showed drives after targeted may represent an effective therapeutic strategy promote functional other diseases.

Language: Английский

Citations

1
Cellular and molecular features of asthma mucus plugs provide clues about their formation and persistence DOI Creative Commons
Maude A. Liegeois, Aileen Hsieh,

May Al-Fouadi

et al.

Journal of Clinical Investigation, Journal Year: 2025, Volume and Issue: 135(6)

Published: March 16, 2025

BACKGROUND. Mucus plugs form in acute asthma and persist chronic disease. Although eosinophils are implicated mechanisms of mucus pathology, many mechanistic details about plug formation persistence unknown.

Language: Английский

Citations

1
Identification of the key DNA damage response genes for predicting immunotherapy and chemotherapy efficacy in lung adenocarcinoma based on bulk, single-cell RNA sequencing, and spatial transcriptomics DOI

Shijie Sun,

Kai Wang,

Deyu Guo

et al.

Computers in Biology and Medicine, Journal Year: 2024, Volume and Issue: 171, P. 108078 - 108078

Published: Feb. 5, 2024

Language: Английский

Citations

7
Proximal and Distal Bronchioles Contribute to the Pathogenesis of Non–Cystic Fibrosis Bronchiectasis DOI
Takanori Asakura, Kenichi Okuda, Gang Chen

et al.

American Journal of Respiratory and Critical Care Medicine, Journal Year: 2023, Volume and Issue: 209(4), P. 374 - 389

Published: Nov. 28, 2023

Rationale: Non–cystic fibrosis bronchiectasis (NCFB) may originate in bronchiolar regions of the lung. Accordingly, there is a need to characterize morphology and molecular characteristics NCFB bronchioles. Objectives: Test hypothesis that exhibits major component disease manifest by mucus plugging ectasia. Methods: Morphologic criteria region-specific epithelial gene expression, measured histologically RNA situ hybridization immunohistochemistry, identified proximal distal bronchioles excised lungs. immunohistochemistry assessed accumulation mucin expression. CRISPR-Cas9–mediated IL-1R1 knockout human bronchial cultures tested IL-1α IL-1β contributions production. Spatial transcriptional profiling characterized Measurements Main Results: Bronchiolar perimeters lumen areas per section area were increased proximal, but not distal, versus control lungs, suggesting bronchiolectasis. In NCFB, was observed ectatic associated nonectatic sections with disease. MUC5AC MUC5B mucins upregulated bronchioles, whereas selectively plugs populated IL-1β–expressing macrophages. sterile sputum supernatants induced expression >80% blocked ablation. upregulation genes secretory cells, hypoxia, interleukin pathways, IL-1β–producing macrophages downregulation ciliogenesis genes. Conclusions: distinctive Both exhibit cell features differ regulation

Language: Английский

Citations

16
Structural Lung Disease and Clinical Phenotype in Bronchiectasis Patients: The EMBARC CT Study DOI
Angelina Pieters, Tjeerd van der Veer, Jennifer J. Meerburg

et al.

American Journal of Respiratory and Critical Care Medicine, Journal Year: 2024, Volume and Issue: 210(1), P. 87 - 96

Published: April 18, 2024

Chest computed tomography (CT) scans are essential to diagnose and monitor bronchiectasis (BE). To date, few quantitative data available about the nature extent of structural lung abnormalities (SLAs) on CT patients with BE.

Language: Английский

Citations

5
Nanomaterials-assisted gene editing and synthetic biology for optimizing the treatment of pulmonary diseases DOI Creative Commons
Lanjie Lei, Wenjie Pan, Xin Shou

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: June 18, 2024

Abstract The use of nanomaterials in gene editing and synthetic biology has emerged as a pivotal strategy the pursuit refined treatment methodologies for pulmonary disorders. This review discusses utilization nanomaterial-assisted tools techniques to promote development more precise efficient treatments diseases. First, we briefly outline characterization respiratory system succinctly describe principal applications diverse lung ailment treatment. Second, elaborate on gene-editing tools, their configurations, assorted delivery methods, while delving into present state nanomaterial-facilitated interventions spectrum Subsequently, expound its deployment biomedicine, focusing research advances diagnosis conditions against backdrop coronavirus disease 2019 pandemic. Finally, summarize extant lacunae current delineate prospects advancement this domain. holistic approach augments pioneering solutions treatment, thereby endowing patients with efficacious personalized therapeutic alternatives.

Language: Английский

Citations

5
Small Airways in Non–Cystic Fibrosis Bronchiectasis DOI Creative Commons
John D. Dickinson, Christopher M. Evans, Burton F. Dickey

et al.

American Journal of Respiratory and Critical Care Medicine, Journal Year: 2024, Volume and Issue: 209(4), P. 347 - 349

Published: Jan. 8, 2024

"Small Airways in Non-Cystic Fibrosis Bronchiectasis." American Journal of Respiratory and Critical Care Medicine, 0(ja), pp.

Language: Английский

Citations

4
Molecular Mechanisms of Immunoinflammatory Infiltration and Ferroptosis in Arthritis Revealed by a Combination of Bioinformatics and Single-Cell Analysis DOI Creative Commons
Chao Song,

Weijun Song,

Yong Liu

et al.

Journal of Inflammation Research, Journal Year: 2025, Volume and Issue: Volume 18, P. 2409 - 2432

Published: Feb. 1, 2025

Osteoarthritis (OA) is a widespread chronic inflammatory disease in orthopedics, and its molecular mechanisms are still poorly understood. The purpose of this work was to detect the immunological infiltration OA manner cell death utilizing bioinformatics single-cell analysis order provide guidelines for clinical therapy medicine. Ferroptosis -associated genes were sourced from ferroptosis Database, bioinformatic expression profiles chosen Gene Expression Comprehensive gene information taken GeneCards. To ascertain categorization status cells, conducted. Protein-protein interaction networks established by SRING analysis, functional enrichment examined Kyoto Encyclopedia Genes Genomes (KEGG) Ontology (GO) databases. important proteins immune-ferroptosis elucidated through co-analysis. Last but not least, network pharmacology docking support mechanism which resveratrol controls OA. development found be tightly related chondrocytes immune particularly T macrophage according profile. In patients with OA, also revealed notable B NK monocytes, macrophages. hub shown enriched responses, chemokine-mediated signaling pathways, analysis. main pathways included autophagy, ferroptosis, HIF-1 pathway, PI3K-Akt FoxO pathway. significant that contributes advancement osteoarthritis. lessened regulation GPX4, TFRC, SLC7A11. Various infiltrates, especially cells macrophages, play an role progression ameliorates modulating chondrocyte ferroptosis.

Language: Английский

Citations

0