bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: July 25, 2024
Viral
entry
is
a
critical
step
in
the
infection
process.
Klebsiella
spp.
and
other
clinically
relevant
bacteria
often
express
complex
polysaccharide
capsule
that
acts
as
barrier
to
phage
entry.
In
turn,
most
phages
encode
depolymerases
for
removal.
This
virus-host
arms
race
has
led
extensive
genetic
diversity
both
capsules
depolymerases,
complicating
our
ability
understand
their
interaction.
study
exploits
information
encoded
prophages
model
interplay
between
bacteria,
prophages,
using
graph
neural
network
sequence
clustering-based
method.
Both
approaches
showed
significant
predictive
capsular
tropism
and,
importantly,
were
transferrable
lytic
phages.
addition
creating
comprehensive
database
linking
depolymerase
sequences
specific
targets,
this
demonstrates
predictability
of
phage-host
interactions
at
subspecies
level,
providing
new
insights
improving
therapeutic
industrial
applicability
Molecules,
Journal Year:
2022,
Volume and Issue:
27(17), P. 5410 - 5410
Published: Aug. 24, 2022
(1)
Background:
Gastric
cancer
(GC)
is
the
fourth
leading
cause
of
cancer-related
deaths
worldwide.
Helicobacter
pylori
infection
a
major
risk
factor,
but
other
microbial
species
may
also
be
involved.
In
context
an
earlier
proteomics
study
serum
and
biopsies
patients
with
gastroduodenal
diseases,
we
explored
here
simplified
microbiome
in
these
(H.
pylori,
Acinetobacter
baumannii,
Escherichia
coli,
Fusobacterium
nucleatum,
Bacteroides
fragilis)
on
protein
level.
(2)
Methods:
A
cohort
75
was
divided
into
groups
respect
to
findings
normal
gastric
mucosa
(NGM)
disorders
such
as
gastritis,
ulcer,
(GC).
The
H.
status
determined.
expression
analysis
biopsy
samples
carried
out
using
high-definition
mass
spectrometry
tryptic
digest
(label-free
data-independent
quantification
statistical
analysis).
(3)
Results:
total
304
bacterial
matches
were
detected
based
two
or
more
peptide
hits.
Significantly
regulated
proteins
like
virulence
factor
type
IV
secretion
system
CagE
from
found
abundance
gastritis
than
GC
NGM.
This
finding
could
reflect
increased
involvement
inflammation
line
current
hypotheses.
Abundant
across
heat
shock
elongation
factors.
(4)
Conclusions:
Next
bulk
human
proteins,
number
species-specific
stomach
some
which,
those
expressed
by
cag
pathogenicity
island,
provide
gateways
disease
prevention
without
antibacterial
intervention
order
reduce
antibiotic
resistance.
Expert Review of Anti-infective Therapy,
Journal Year:
2024,
Volume and Issue:
unknown, P. 1 - 17
Published: Oct. 9, 2024
complex
(Abc)
is
currently
a
significant
cause
of
difficult-to-treat
pneumonia.
Due
to
the
high
prevalence
rates
carbapenem-
and
extensively
drug-resistant
(CR,
XDR)
phenotypes,
limited
antibiotic
options
are
available
for
effective
treatment
pneumonia
caused
by
CR/XDR-Abc.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: July 25, 2024
Viral
entry
is
a
critical
step
in
the
infection
process.
Klebsiella
spp.
and
other
clinically
relevant
bacteria
often
express
complex
polysaccharide
capsule
that
acts
as
barrier
to
phage
entry.
In
turn,
most
phages
encode
depolymerases
for
removal.
This
virus-host
arms
race
has
led
extensive
genetic
diversity
both
capsules
depolymerases,
complicating
our
ability
understand
their
interaction.
study
exploits
information
encoded
prophages
model
interplay
between
bacteria,
prophages,
using
graph
neural
network
sequence
clustering-based
method.
Both
approaches
showed
significant
predictive
capsular
tropism
and,
importantly,
were
transferrable
lytic
phages.
addition
creating
comprehensive
database
linking
depolymerase
sequences
specific
targets,
this
demonstrates
predictability
of
phage-host
interactions
at
subspecies
level,
providing
new
insights
improving
therapeutic
industrial
applicability
