Spatiotemporal Variability of the Pepper Mild Mottle Virus Biomarker in Wastewater DOI Creative Commons

AnnaElaine L. Rosengart,

Amanda L. Bidwell, Marlene K. Wolfe

et al.

ACS ES&T Water, Journal Year: 2024, Volume and Issue: 5(1), P. 341 - 350

Published: Dec. 16, 2024

Since the start of coronavirus-19 pandemic, use wastewater-based epidemiology (WBE) for disease surveillance has increased throughout world. Because wastewater measurements are affected by external factors, processing WBE data typically includes a normalization step in order to adjust (e.g., viral ribonucleic acid (RNA) concentrations) account variation due dynamic population changes, sewer travel effects, or laboratory methods. Pepper mild mottle virus (PMMoV), plant RNA abundant human feces and wastewater, been used as fecal contamination indicator normalize extensively. However, there little work characterize spatiotemporal variability PMMoV which may influence effectiveness adjusting normalizing measurements. Here, we investigate its across space time using collected over two-year period from sewage treatment plants United States. We find that most can be attributed longitude latitude followed site-specific variables. Further research into cross-geographical -temporal comparability PMMoV-normalized pathogen concentrations would strengthen utility WBE.

Language: Английский

Detection of Hemagglutinin H5 Influenza A Virus Sequence in Municipal Wastewater Solids at Wastewater Treatment Plants with Increases in Influenza A in Spring, 2024 DOI Creative Commons
Marlene K. Wolfe,

Dorothea Duong,

Bridgette Shelden

et al.

Environmental Science & Technology Letters, Journal Year: 2024, Volume and Issue: 11(6), P. 526 - 532

Published: May 20, 2024

Prospective influenza A (IAV) RNA monitoring at 190 wastewater treatment plants (WWTPs) across the US identified increases in IAV concentrations 59 spring 2024, after typical seasonal period, coincident with identification of highly pathogenic avian (subtype H5N1) circulating dairy cattle US. We developed and validated a hydrolysis-probe RT-PCR assay for quantification H5 hemagglutinin gene. applied it retrospectively to samples from four WWTPs where springtime were one WWTP they not. The marker was detected all coinciding not without an increase. Positive are located states confirmed outbreaks influenza, H5N1 clade 2.3.4.4b, cattle. Concentrations gene approached overall virus concentrations, suggesting large fraction inputs subtypes. At positive WWTPs, industrial discharges containing animal waste, including milk byproducts, permitted discharge into sewers. Our findings demonstrate that can detect animal-associated contributions highlight need consider agricultural wastewater. This work illustrates monitoring's value comprehensive surveillance, influenzas currently thought be primarily found animals important implications human health.

Language: Английский

Citations

24
Comparative impact of sunlight and salinity on human pathogenic virus survival in river, estuarine, and marine water microcosms DOI Creative Commons
Jessica L. Kevill,

Kate Herridge,

Xiaorong Li

et al.

Water Research, Journal Year: 2025, Volume and Issue: unknown, P. 123411 - 123411

Published: Feb. 1, 2025

Language: Английский

Citations

1
Adult Long Coronavirus Disease 2019 DOI
Ivette F. Emery, Clifford J. Rosen

Infectious Disease Clinics of North America, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

Citations

1
Human pathogen nucleic acids in wastewater solids from 191 wastewater treatment plants in the United States DOI Creative Commons
Alexandria B. Boehm, Marlene K. Wolfe, Amanda L. Bidwell

et al.

Scientific Data, Journal Year: 2024, Volume and Issue: 11(1)

Published: Oct. 17, 2024

We measured concentrations of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its variants, influenza A B viruses, syncytial virus, human metapneumovirus, enterovirus D68, parainfluenza types 1, 2, 3, 4a, 4b in aggregate, norovirus genotype II, rotavirus, Candida auris, hepatitis adenovirus, mpox H5 pepper mild mottle virus nucleic acids wastewater solids prospectively at 191 treatment plants 40 states across the United States plus Washington DC. Measurements were made two to seven times per week from 1 January 2022 30 June 2024, depending on plant staff availability. using droplet digital (reverse-transcription-) polymerase chain reaction (ddRT-PCR) following best practices for making environmental molecular biology measurements. These data can be used better understand disease occurrence communities contributing wastewater.

Language: Английский

Citations

6
Detection of hemagglutinin H5 influenza A virus sequence in municipal wastewater solids at wastewater treatment plants with increases in influenza A in spring, 2024 DOI
Marlene K. Wolfe,

Dorothea Duong,

Bridgette Shelden

et al.

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: April 29, 2024

Abstract Prospective influenza A (IAV) RNA monitoring at 190 wastewater treatment plants across the US identified increases in IAV concentrations 59 spring 2024, after typical seasonal period, coincident with identification of highly pathogenic avian (subtype H5N1) circulating dairy cattle US. We developed and validated a hydrolysis-probe RT-PCR assay for quantification H5 hemagglutinin gene. applied it retrospectively to samples from three where springtime were identified. The marker was detected all coinciding increases. Plants located state confirmed outbreaks influenza, H5N1 clade 2.3.4.4b, cattle. Concentrations gene approached overall virus concentrations, suggesting large fraction inputs subtypes. At two plants, industrial discharges containing animal waste, including milk byproducts, permitted discharge into sewers. Our findings demonstrate can detect animal-associated contributions, highlight need consider agricultural wastewater. This work illustrates monitoring’s value comprehensive surveillance diseases zoonotic potential human populations.

Language: Английский

Citations

4
Wastewater-Based Surveillance of Respiratory Syncytial Virus Reveals a Temporal Disconnect in Disease Trajectory across an Active International Land Border DOI Creative Commons
Mackenzie Beach, Ryland Corchis-Scott, Qiudi Geng

et al.

Environment & Health, Journal Year: 2025, Volume and Issue: 3(4), P. 425 - 435

Published: Jan. 29, 2025

Conventional metrics for tracking infectious diseases, including case and outbreak data syndromic surveillance, can be resource-intensive, misleading, comparatively slow with prolonged collection, analysis authentication. This study examined the 2022–2023 Respiratory Syncytial Virus (RSV) season in a contiguous metropolitan area connected by an active international land border, affording opportunity comparison of respiratory virus spanning two independent public health jurisdictions. Time-lagged cross correlation qualitative examination wastewater signals showed that peak Detroit (MI, USA) RSV predated Windsor (ON, Canada) approximately 5 weeks. A strong positive relationship was observed between N-gene concentrations hospitalization rates Windsor-Essex (Kendall's τ = 0.539, p ≤ 0.001, Spearman's ρ 0.713, 0.001) as well 0.739, 0.888, 0.001). demonstrated surveillance reveal regional differences infection dynamics communities provide measure prevalence RSV, underreported disease. These findings support use cost-effective tool monitoring to enhance existing systems better inform disease mitigation strategies.

Language: Английский

Citations

0
Wastewater-based effective reproduction number and prediction under the absence of shedding information DOI Creative Commons
Hiroki Ando, Kelly A. Reynolds

Environment International, Journal Year: 2024, Volume and Issue: 194, P. 109128 - 109128

Published: Nov. 14, 2024

Estimating effective reproduction number (R

Language: Английский

Citations

0
Spatiotemporal Variability of the Pepper Mild Mottle Virus Biomarker in Wastewater DOI Creative Commons

AnnaElaine L. Rosengart,

Amanda L. Bidwell, Marlene K. Wolfe

et al.

ACS ES&T Water, Journal Year: 2024, Volume and Issue: 5(1), P. 341 - 350

Published: Dec. 16, 2024

Since the start of coronavirus-19 pandemic, use wastewater-based epidemiology (WBE) for disease surveillance has increased throughout world. Because wastewater measurements are affected by external factors, processing WBE data typically includes a normalization step in order to adjust (e.g., viral ribonucleic acid (RNA) concentrations) account variation due dynamic population changes, sewer travel effects, or laboratory methods. Pepper mild mottle virus (PMMoV), plant RNA abundant human feces and wastewater, been used as fecal contamination indicator normalize extensively. However, there little work characterize spatiotemporal variability PMMoV which may influence effectiveness adjusting normalizing measurements. Here, we investigate its across space time using collected over two-year period from sewage treatment plants United States. We find that most can be attributed longitude latitude followed site-specific variables. Further research into cross-geographical -temporal comparability PMMoV-normalized pathogen concentrations would strengthen utility WBE.

Language: Английский

Citations

0