Bacterial biofilms: from the Natural environment to infectious diseases

Nature Reviews Microbiology, Journal Year: 2004, Volume and Issue: 2(2), P. 95 - 108

Published: Jan. 14, 2004

Language: Английский

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Microbial Biofilms: from Ecology to Molecular Genetics

Microbiology and Molecular Biology Reviews, Journal Year: 2000, Volume and Issue: 64(4), P. 847 - 867

Published: Dec. 1, 2000

SUMMARY Biofilms are complex communities of microorganisms attached to surfaces or associated with interfaces. Despite the focus modern microbiology research on pure culture, planktonic (free-swimming) bacteria, it is now widely recognized that most bacteria found in natural, clinical, and industrial settings persist association surfaces. Furthermore, these microbial often composed multiple species interact each other their environment. The determination biofilm architecture, particularly spatial arrangement microcolonies (clusters cells) relative one another, has profound implications for function communities. Numerous new experimental approaches methodologies have been developed order explore metabolic interactions, phylogenetic groupings, competition among members biofilm. To complement this broad view ecology, individual organisms studied using molecular genetics identify genes required development dissect regulatory pathways control plankton-to-biofilm transition. These genetic studies led emergence concept formation as a novel system study bacterial development. recent explosion field exciting progress technologies studying communities, advanced our understanding ecological significance surface-attached provided insights into basis

Language: Английский

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Diffusion in Biofilms

Journal of Bacteriology, Journal Year: 2003, Volume and Issue: 185(5), P. 1485 - 1491

Published: Feb. 18, 2003

Much of what makes life in a microbial biofilm different from free aqueous suspension can be explained by invoking the phenomenon diffusion. This article discusses profound influence physics diffusion process on chemistry and biology mode growth.

Language: Английский

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Contributions of Antibiotic Penetration, Oxygen Limitation, and Low Metabolic Activity to Tolerance of Pseudomonas aeruginosa Biofilms to Ciprofloxacin and Tobramycin

Antimicrobial Agents and Chemotherapy, Journal Year: 2002, Volume and Issue: 47(1), P. 317 - 323

Published: Dec. 24, 2002

ABSTRACT The roles of slow antibiotic penetration, oxygen limitation, and low metabolic activity in the tolerance Pseudomonas aeruginosa biofilms to killing by antibiotics were investigated vitro. Tobramycin ciprofloxacin penetrated but failed effectively kill bacteria. Bacteria colony survived prolonged exposure either 10 μg tobramycin ml −1 or 1.0 . After 100 h treatment, during which transferred fresh antibiotic-containing plates every 24 h, log reduction viable cell numbers was only 0.49 ± 0.18 for 1.42 0.03 ciprofloxacin. Antibiotic permeation through biofilms, indicated a diffusion bioassay, demonstrated that there no acceleration bacterial once biofilms. These results suggested limited is not primary protective mechanism these Transmission electron microscopic observations antibiotic-affected cells showed lysed, vacuolated, elongated exclusively near air interface antibiotic-treated suggesting role limitation protecting biofilm bacteria from antibiotics. To test this hypothesis, microelectrode analysis performed. 50 90 μm into interface. This oxic zone correlated region where an inducible green fluorescent protein expressed, indicating active activity. show interior biofilm, poor are with P. system.

Language: Английский

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[6] Genetic approaches to study of biofilms

Methods in enzymology on CD-ROM/Methods in enzymology, Journal Year: 1999, Volume and Issue: unknown, P. 91 - 109

Published: Jan. 1, 1999

Language: Английский

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Biofilm dispersion

Nature Reviews Microbiology, Journal Year: 2020, Volume and Issue: 18(10), P. 571 - 586

Published: June 12, 2020

Language: Английский

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Surface-Adaptive Gold Nanoparticles with Effective Adherence and Enhanced Photothermal Ablation of Methicillin-Resistant Staphylococcus aureus Biofilm

ACS Nano, Journal Year: 2017, Volume and Issue: 11(9), P. 9330 - 9339

Published: Aug. 14, 2017

Biofilms that contribute to the persistent bacterial infections pose serious threats global public health, mainly due their resistance antibiotics penetration and escaping innate immune attacks by phagocytes. Here, we report a kind of surface-adaptive gold nanoparticles (AuNPs) exhibiting (1) self-adaptive target acidic microenvironment biofilm, (2) an enhanced photothermal ablation methicillin-resistant Staphylococcus aureus (MRSA) biofilm under near-infrared (NIR) light irradiation, (3) no damage healthy tissues around biofilm. Originally, AuNPs were readily prepared surface modification with pH-responsive mixed charged zwitterionic self-assembled monolayers consisting weak electrolytic 11-mercaptoundecanoic acid (HS-C10-COOH) strong (10-mercaptodecyl)trimethylammonium bromide (HS-C10-N4). The zwitterion-modified showed fast transition from negative charge positive charge, which enabled disperse well in (pH ∼7.4), while quickly presenting adherence negatively bacteria surfaces MRSA ∼5.5). Simultaneous AuNP aggregation within NIR irradiation. surrounding because dispersed had effect light. In view above advantages as straightforward preparation, developed this work may find potential applications useful antibacterial agent areas healthcare.

Language: Английский

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Heterogeneity in biofilms: Table 1

FEMS Microbiology Reviews, Journal Year: 2000, Volume and Issue: 24(5), P. 661 - 671

Published: Dec. 1, 2000

Biofilms, accumulations of microorganisms at interfaces, have been described for every aqueous system supporting life. The structure these microbial communities ranges from monolayers scattered single cells to thick, mucous structures macroscopic dimensions (microbial mats; algal-microbial associations; trickling filter biofilms). During recent years the biofilms many different environments has documented and evaluated by use a broad variety microscopic, physico-chemical molecular biological techniques, revealing generally complex 3D structure. Parallel investigations more mathematical models simulations were developed explain development, structures, interactions biofilms. forces determining spatial biofilms, including microcolonies, extracellular polymeric substances (EPS), channels, are still subject controversy. To achieve conclusive explanations observed in cooperation both fields investigation, modelling experimental research, is necessary. expanding field techniques not only allows detailed documentation distribution species, but also functional activities their biofilm environment. These new methods will certainly reveal insights mechanisms involved developmental processes formation behavior

Language: Английский

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Isolation of an Escherichia coli K-12 Mutant Strain Able To Form Biofilms on Inert Surfaces: Involvement of a New ompR Allele That Increases Curli Expression

Journal of Bacteriology, Journal Year: 1998, Volume and Issue: 180(9), P. 2442 - 2449

Published: May 1, 1998

ABSTRACT Classical laboratory strains of Escherichia coli do not spontaneously colonize inert surfaces. However, when maintained in continuous culture for evolution studies or industrial processes, these usually generate adherent mutants which form a thick biofilm, visible with the naked eye, on wall apparatus. Such mutant was isolated to identify genes and morphological structures involved biofilm formation very well characterized E. K-12 context. This acquired ability hydrophilic (glass) hydrophobic (polystyrene) surfaces aggregation clumps. A single point mutation, resulting replacement leucine by an arginine residue at position 43 regulatory protein OmpR, responsible this phenotype. Observations electron microscopy revealed presence bacteria fibrillar looking like particular fimbriae described Olsén group designated curli (A. Olsén, A. Jonsson, S. Normark, Nature 338:652–655, 1989). The production (visualized Congo red binding) expression csgA gene encoding curlin synthesis (monitored coupling reporter its promoter) were significantly increased ompR allele work. Transduction knockout mutations either caused loss adherence properties several biofilm-forming strains, including all those work from apparatus two clinical patients catheter-related infections. These results indicate that are major importance surface colonization demonstrate their is under control EnvZ-OmpR two-component system.

Language: Английский

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Overview of microbial biofilms

Journal of Industrial Microbiology, Journal Year: 1995, Volume and Issue: 15(3), P. 137 - 140

Published: Sept. 1, 1995

Language: Английский

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