mSphere, Journal Year: 2024, Volume and Issue: unknown
Published: Dec. 11, 2024
Mathematical models can provide insights into complex interactions and dynamics within microbial communities to complement extend experimental laboratory approaches. For dental biofilms, they give a basis for evaluating biofilm growth or the transition from health disease. We have developed mathematical simulate toward cariogenic biofilm, modeled as overgrowth of
Language: Английский
Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown
Published: Nov. 16, 2024
Abstract Implant‐related infections are characterized by the formation of bacterial biofilms. Current treatments have various drawbacks. Nanozymes with enzyme‐like activity can produce highly toxic substances to kill bacteria and remove biofilms without inducing drug resistance. However, it is difficult for current monometallic nanozymes function well in complex biofilm environments. Therefore, development multimetallic efficient multienzyme activities crucial. In present study, bimetallic nanozyme, ZnO‐CuS nanoflowers peroxidase (POD), glutathione oxidase (GSH‐Px), catalase (CAT) successfully synthesized via calcination loaded into F127 hydrogels treatment implant‐related infections. The ability bind key antimicrobial activity. addition, H 2 O disrupt metabolism MRSA , including arginine synthesis, nucleotide excision repair, energy metabolism, protein synthesis. ZnO‐CuS/F127 hydrogel combination has been demonstrated be effective clearing infection facilitating switch M1 macrophages M2‐repairative phenotype implant mice. Furthermore, favorable biosafety, their toxicity negligible. provided a promising biomedical strategy healing infections, highlighting potential clinical applications.
Language: Английский
Citations
12
Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 14
Published: Jan. 22, 2025
The Gram-positive organism Filifactor alocis is implicated in multiple oral diseases including periodontitis, and approximately 50% of known strains encode produce a recently identified repeat-in-toxin (RTX) protein, FtxA, partly homologous to the Aggregatibacter actinomycetemcomitans leukotoxin. By assessing longitudinal Ghanaian study population adolescents, we possible correlation between F. levels, ftxA gene carriage, progression clinical attachment loss (CAL). To extend knowledge on significance its FtxA periodontal disease, have present work analyzed saliva samples an independent cohort periodontitis (n=156), collected at two private specialist practices Perth, Western Australia. results corroborate that high loads presence together are associated with parameters tissue destruction severity. Moreover, among individuals carrying A. , majority also exhibited -positive supporting notion synergistic behavior these species. This emphasizes involved pathogenesis may ecological roles, diagnostic prognostic implications for disease.
Language: Английский
Citations
1
Oral Diseases, Journal Year: 2024, Volume and Issue: 30(7), P. 4069 - 4077
Published: May 30, 2024
Periodontitis is an inflammatory oral disease that occurs as a result of the damaging effects immune response against subgingival microflora. Among mechanisms involved, nucleotide-binding oligomerization domain, leucine-rich repeat-containing proteins family member NLRP3 (NLR pyrin domain-containing 3), proposed key regulator macrophage-induced inflammation, strongly associated with periodontal due to bacterial activators. This paper aimed present general concepts inflammasome activation and regulation in disease.
Language: Английский
Citations
7
Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 12
Published: Jan. 7, 2025
Advances in tissue engineering and microfluidic technologies have enabled the development of sophisticated vitro models known as organ-on-a-chip (OoC) or microphysiological systems. These systems enable to potential simulate dynamic interactions between host tissues their microenvironment including microbes, biomaterials, mechanical forces, pharmaceutical, consumer-care products. fluidic are increasingly being utilized investigate host-microbe host-material oral health disease. Of interest is application understanding periodontal disease, a chronic inflammatory condition marked by progressive destruction tissues, gingiva, ligament, alveolar bone. The pathogenesis disease involves complex interplay microbial dysbiosis immune responses, which can lead loss dental support structures contribute systemic conditions such cardiovascular diabetes, bowel This provides comprehensive overview latest developments millifluidic designed emulate interactions. We discuss critical biological considerations designing these platforms, applications studying biofilms, unravel mechanisms therapeutic targets
Language: Английский
Citations
0
npj Biofilms and Microbiomes, Journal Year: 2025, Volume and Issue: 11(1)
Published: Jan. 9, 2025
The human body houses many distinct and interconnecting microbial populations with long-lasting systemic effects, where the oral cavity serves as a pathogens' reservoir. correlation of different disease states strongly supports need to understand interplay between tissue niche microbiome. Despite efforts, recapitulation gingival architecture physiological characteristics periodontal has yet be accomplished by traditional cultural strategies. Here, we are showing for first time investigation host–microbiome interactions in healthy conditions within model over seven days. Our results indicated long-term host microbiome viability, barrier integrity, phenotypic functional response, preservation interbacterial dialogs. This vitro platform can maintain homeostasis at interface niche, thus, offering opportunities identify predictive biomarkers develop intervention strategies promote overall health.
Language: Английский
Citations
0
Frontiers in Oral Health, Journal Year: 2025, Volume and Issue: 6
Published: Feb. 11, 2025
Introduction Salivary mucin MUC5B has been suggested to support eubiosis in early oral biofilms by regulating the attachment of commensals, while downregulating dysbiotic activities related dental caries development, such as microbial carbohydrate transport and metabolism. Methods To investigate how metabolism glucose, a potential driver for caries, mono- dual-species Actinomyces naeslundii Streptococcus gordonii clinical isolates was affected presence complex salivary MUC5B, this study employed nuclear magnetic resonance (NMR)-based metabolomics with interpretation network integration. Results discussion reduced glucose compared uncoated surfaces but maintained even species distribution. This suggests that may represent an innate mechanism regulate biofilm supporting coadhesion total biomass. All annotated metabolites were intermediates either metabolism, pyruvate conversion, or amino acid which not unexpected metabolomes from two saccharolytic since conversion represents junction point between glycolysis metabolic chains. The 10 present all core metabolome shared A. S. . Such can be used detect deviations future studies. Significant differences metabolite abundance elicited also detected. In where they each present, pyruvate, ethanol, 134 significantly higher 25% 20 mM (MUC5B + G) physiologic buffer (PBS G), 84, 97, sarcosine at lower abundance. Metabolite 72 unique grown G, eight unannotated PBS G. A pathway enrichment analysis differently expressed , without showed over-represented. Studying interactions commensal members modulatory effects host factors glycoproteins saliva during substrates are drivers dysbiosis, is essential understand roles ecosystems health disease.
Language: Английский
Citations
0
Infection and Immunity, Journal Year: 2025, Volume and Issue: unknown
Published: April 2, 2025
Treponema denticola is an obligate colonizer of the human gingival crevice and, along with other pathobionts, highly associated development periodontal disease. As disease develops, significant environmental changes occur in subgingival and oral microbiome. The ability to sense respond changing conditions essential T. thrive cause Yet, our understanding sensory transduction gene regulatory mechanisms nearly absent. AtcSR two-component system has been predicted regulate several cellular processes, but its role adaptive responses not investigated. To address this knowledge gap, we constructed a deletion atcS gene, encoding histidine kinase. We performed RNA sequencing, demonstrating that results transcriptome denticola. Most notably, transcription genes proteins involved motility dentilisin protease complex was reduced. Consistent this, mutant displayed reduced activity motility. These phenotypes are critical interactions host cells pathogenicity This aligns observation atcS-deficient strain had attenuated attachment invasion epithelial failed induce alveolar bone loss murine periodontitis model, processes central virulence. study step toward defining pathogenicity.
Language: Английский
Citations
0
Advanced Science, Journal Year: 2025, Volume and Issue: unknown
Published: May 14, 2025
Abstract A mutualistic co‐evolution exists between the host and its associated microbiota in human body. Bacteria establish ecological niches various tissues of body, locally influencing their physiology functions, but also contributing to well‐being whole organism through systemic communication with other distant (axis). Emerging evidence indicates that when composition inhabiting niche changes toward a pathogenic state (dysbiosis) interactions become unbalanced, diseases may present. In addition, imbalances within single can cause dysbiosis organs. Current research efforts are focused on elucidating mechanisms leading dysbiosis, goal restoring tissue homeostasis. vitro models provide critical experimental platforms address this need, by reproducing cyto‐architecture high fidelity. This review surveys current host–microbiota strategies provides roadmap guide field further developing physiologically relevant niches, thus enabling investigation role health diseases. Lastly, given Food Drug Administration Modernization Act 2.0, highlights emerging support development validation new therapies market.
Language: Английский
Citations
0
Frontiers in Biomaterials Science, Journal Year: 2024, Volume and Issue: 3
Published: July 12, 2024
Periodontitis is a chronic inflammatory disease associated with dysbiosis in subgingival plaque biofilm, characterised by damage to the periodontal tissues, eventually leading tooth loss. Hence, pathophysiology of periodontitis and interaction between host tissue under various environmental cues are central pathogenesis periodontitis. Therefore, engineering biofilm models that mimic vivo crucial obtaining clear insight into pathology developing targeted therapeutic methods. In this review, we provide comprehensive overview strategies employed modelling oral biofilms focusing on surface attachment, fluid microenvironment, gas environment, shear force, microbial-host offer insights ongoing challenges future perspectives, which will enable development novel physiological relevant for biofilms.
Language: Английский
Citations
3
Frontiers in Oral Health, Journal Year: 2024, Volume and Issue: 5
Published: April 26, 2024
Accumulating microbiome data and mechanistic studies in vitro vivo have refined our understanding of the oral microbiota as a functionally integrated polymicrobial community. Emergent properties these communities are driven to large extent by interspecies communication which can be based on physical association, secreted small molecules or nutritional exchange. Porphyromonas gingivalis is consensus periodontal pathogen; however, virulence only expressed context Multivalent fimbriae mediate attachment other species initiate distinct transcriptional program both constituents binding pair. P. also responds cues produced partner organisms. Physiological interdependence forms basis complex networks cooperating organisms begin resemble an organismal entity exhibiting spectrum pathogenic potential.
Language: Английский
Citations
2