Nectin1 is a pivotal host factor involved in attachment and entry of red-spotted grouper nervous necrosis virus in the early stages of the viral life cycle

Journal of Virology, Journal Year: 2024, Volume and Issue: 98(9)

Published: Aug. 28, 2024

Nervous necrosis virus (NNV) is a highly neurotropic that poses persistent threat to the survival of multiple fish species. However, its inimitable neuropathogenesis remains largely elusive. To rummage potential partners germane nervous system, we investigated interaction between red-spotted grouper NNV (RGNNV) and brain by immunoprecipitation coupled with mass spectrometry discerned Nectin1 as novel host factor subtly involved in viral early invasion events. was abundant neural tissues implicated inception tunnel nanotubes triggered RGNNV. Its overexpression not only dramatically potentiated replication dynamics RGNNV susceptible cells, but also empowered non-sensitive cells expeditiously capture free virions within 2 min. This potency impervious low temperatures dose-dependently suppressed soluble protein or specific antibody ectodomain, indicating an attachment receptor for Mechanistically, efficient hijacking strictly depended on intricate linkages different modules capsid protein, especially direct binding IgC1 loop P-domain. More strikingly, despite abortive proliferation Nectin1-reconstructed CHSE-214 cell, could gain access intracellular compartment capitalizing Nectin1, thereby inducing canonical cytoplasmic vacuolation. Altogether, our findings delineate candidate entrance infiltration into which may shed unprecedented insights exploration elucidation pathogenesis.IMPORTANCENervous one most virulent pathogens aquaculture industry, inflicts catastrophic damage ecology, environment, economy annually around world. Nevertheless, idiosyncratic latency mechanisms pose enormous hardships epidemic prevention control. In this study, deploying natural screening library, single-transmembrane glycoprotein, first identified emergent functional widely allocated directly interacted through distinct Ig-like loops bridge virus-host crosstalk, apprehend virions, concomitantly propel entry. Our illuminate critical role entry provide target future clinical intervention strategies therapeutic race against

Language: Английский

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Research Advances on Swine Acute Diarrhea Syndrome Coronavirus

Animals, Journal Year: 2024, Volume and Issue: 14(3), P. 448 - 448

Published: Jan. 30, 2024

Swine acute diarrhea syndrome coronavirus (SADS-CoV) is a virulent pathogen that causes in piglets. The virus was first discovered Guangdong Province, China, 2017 and has since emerged Jiangxi, Fujian, Guangxi Provinces. outbreak exhibited localized sporadic pattern, with no discernable temporal continuity. can infect human progenitor cells demonstrates considerable potential for cross-species transmission, representing risk zoonotic transmission. Therefore, continuous surveillance of comprehensive research on SADS-CoV are imperative. This review provides an overview the evolutionary features outbreaks, focusing structural characteristics virus, which serve as basis discussing its interspecies Additionally, summarizes virus–host interactions, including effects host cells, well apoptotic autophagic behaviors, discusses prevention treatment modalities this viral infection.

Language: Английский

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ALIX and TSG101 are essential for cellular entry and replication of two porcine alphacoronaviruses

PLoS Pathogens, Journal Year: 2024, Volume and Issue: 20(3), P. e1012103 - e1012103

Published: March 15, 2024

Alphacoronaviruses are the primary coronaviruses responsible for causing severe economic losses in pig industry with potential to cause human outbreaks. Currently, extensive studies have reported essential role of endosomal sorting and transport complexes (ESCRT) life cycle enveloped viruses. However, very little information is available about which ESCRT components crucial alphacoronaviruses infection. By using RNA interference combination Co-immunoprecipitation, as well fluorescence electron microscopy approaches, we dissected ALIX TSG101 two porcine alphacoronavirus cellular entry replication. Results show that infection by alphacoronaviruses, including epidemic diarrhea virus (PEDV) enteric (PEAV), dramatically decreased ALIX- or TSG101-depleted cells. Furthermore, PEDV entr y significantly increases interaction caveolin-1 (CAV1) RAB7, viral endocytosis lysosomal transport, however, does not require TSG101. Interestingly, PEAV only relies on regulate but also requires macropinocytosis. Besides, recruited replication sites where they become localized within endoplasmic reticulum virus-induced double-membrane vesicles. were inhibited depletion Vero cells jejunal epithelial cells, indicating Collectively, these data highlight dual alphacoronaviruses. Thus, proteins could serve therapeutic targets against

Language: Английский

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Micropterus salmoides rhabdovirus enters cells via clathrin-mediated endocytosis pathway in a pH-, dynamin-, microtubule-, rab5-, and rab7-dependent manner

Journal of Virology, Journal Year: 2023, Volume and Issue: 97(10)

Published: Sept. 22, 2023

Although

Language: Английский

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Cholesterol-dependent Nsp5-endosomes co-trafficking to lysosomes facilitates Porcine Reproductive and Respiratory Syndrome Replication by activating autophagy

Veterinary Microbiology, Journal Year: 2025, Volume and Issue: unknown, P. 110507 - 110507

Published: April 1, 2025

Language: Английский

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Rab5-mediated phagocytosis restricts Spiroplasma eriocheiris infection in crabs through a ubiquitination-dependent mechanism

Aquaculture, Journal Year: 2025, Volume and Issue: unknown, P. 742635 - 742635

Published: April 1, 2025

Language: Английский

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Swine interferon-induced transmembrane proteins inhibit porcine epidemic diarrhea virus replication

Veterinary Microbiology, Journal Year: 2025, Volume and Issue: unknown, P. 110495 - 110495

Published: May 1, 2025

Language: Английский

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Quercetin inhibition of porcine intestinal alpha coronavirus in vitro and in vivo

BMC Veterinary Research, Journal Year: 2024, Volume and Issue: 20(1)

Published: April 3, 2024

Porcine acute diarrhea syndrome coronavirus (SADS-CoV) is one of the novel pathogens responsible for piglet diarrhea, contributing to substantial economic losses in farming sector. The broad host range SADS-CoV raises concerns regarding its potential cross-species transmission. Currently, there are no effective means preventing or treating infection, underscoring urgent need identifying efficient antiviral drugs. This study focuses on evaluating quercetin as an agent against SADS-CoV.

Language: Английский

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Rab7 GTPase, a direct target of miR-131-3p, limits intracellular Spiroplasma eriocheiris infection by modulating phagocytosis

Fish & Shellfish Immunology, Journal Year: 2024, Volume and Issue: 154, P. 109879 - 109879

Published: Sept. 6, 2024

Language: Английский

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Autophagy promotes p72 degradation and capsid disassembly during the early phase of African swine fever virus infection

Journal of Virology, Journal Year: 2024, Volume and Issue: 99(1)

Published: Dec. 17, 2024

During viral infections, autophagy functions as a cell-intrinsic defense mechanism by facilitating the delivery of virions or components to endosomal/lysosomal pathway for degradation. In this study, we report that internalized African swine fever virus (ASFV) enter autolysosomes during early phase infection. Autophagy selectively targets major capsid protein p72 within ASFV virion. The undergoes modification through ubiquitination at C-terminus, process mediated E3 ubiquitin ligase Stub1. Subsequently, ubiquitinated is recognized receptor SQSTM1/p62 its ubiquitin-binding domain. Stub1 facilitates and degradation in an HSPA8-dependent manner via selective autophagy. plays critical role disassembling further promotes release genomic DNA. These findings support notion involved contributes disassembly ASFV, providing valuable insights into essential process.IMPORTANCEAfrican (ASF), highly contagious disease caused ASF (ASFV), affects domestic pigs wild boars, with mortality rate up 100%. epidemic poses persistent threat global pig industry. Currently, no effective vaccines antiviral drugs are available prevention control. discovered Mechanically, polyubiquitination chaperone HSPA8. polyubiquitinated then interacts SQSTM1/p62, leading pathway. reveal provide new ASFV.

Language: Английский

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