Effect of β-Lactam Antibiotics on the Gut Microbiota of Term Neonates DOI Creative Commons

Hongdan Gu,

Enfu Tao,

Yijia Fan

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: May 7, 2024

Abstract β-Lactam antibiotics are a class of that commonly used to treat bacterial infections. However, the effects β-lactam on term neonatal intestinal flora have not been fully elucidated. Hospitalized full-term newborns receiving formed antibiotic group, while those without treatment comprised non-antibiotic group. A healthy group included newborns. Stool samples were collected for 16S rDNA sequencing analyze gut microbiota variations. Further investigation was carried out within exploring use newborns’ in relation duration and type administration, delivery method, feeding practices. The exhibited significant difference microbial beta diversity compared other groups. Genera like Klebsiella, Enterococcus,Streptococcus, Alistipes, Aeromonas enriched, Escherichia-Shigella, Clostridium sensu stricto 1, Bifidobacterium, and Parabacteroideswere reduced. Klebsiella negatively correlated with positively Enterobacter, Escherichia-Shigella Enterococcus and Streptococcus. Regardless age, induced elevated andEnterococcus. Impact varied (cefotaxime or ampicillin/sulbactam). Compared vaginal delivery, cesarean after heightened abundance Enterobacteriaceae_Unclassified, Lactobacillales_Unclassified, Pectobacterium. Feeding patterns minimally influenced β-lactam-induced alterations. In conclusion, β-lactam pneumonia sepsis markedly disrupted microbiota, favoring Enterococcus, Streptococcus, Aeromonas. impact by duration, type, emphasizing disruptions post-cesarean delivery.

Language: Английский

Clinical and laboratory insights into the threat of hypervirulent Klebsiella pneumoniae DOI

Xinhong Han,

Jiayao Yao, Jintao He

et al.

International Journal of Antimicrobial Agents, Journal Year: 2024, Volume and Issue: 64(3), P. 107275 - 107275

Published: July 14, 2024

Language: Английский

Citations

12
Animal models of Klebsiella pneumoniae mucosal infections DOI Creative Commons
Lucas Assoni,

Ana Julia Melo Couto,

Brenda Vieira

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: March 15, 2024

Klebsiella pneumoniae is among the most relevant pathogens worldwide, causing high morbidity and mortality, which worsened by increasing rates of antibiotic resistance. It a constituent host microbiota different mucosa, that can invade cause infections in many sites. The development new treatments prophylaxis against this pathogen rely on animal models to identify potential targets evaluate efficacy possible side effects therapeutic agents or vaccines. However, validity data generated highly dependable choosing adequately reproduce hallmarks human diseases. present review summarizes current knowledge used investigate K. infections, with focus mucosal advantages limitations each model are discussed compared; applications, extrapolations subjects future modifications improve techniques also presented. While mice widely species studies, they such as natural resistance difficulties reproducing main steps infections. Other models, Drosophila melanogaster (fruit fly), Caenorhabditis elegans, Galleria mellonella Danio rerio (zebrafish), contribute understanding specific aspects infection process, bacterial lethality colonization innate immune system response, however, but do not immunological complexity mammals. In conclusion, choice will depend mainly questions being addressed study, while better interplay between virulence factors responses provide deeper comprehension disease process aid effective preventive/therapeutic strategies.

Language: Английский

Citations

10
In vivo single-cell high-dimensional mass cytometry analysis to track the interactions between Klebsiella pneumoniae and myeloid cells DOI Creative Commons
Ricardo Calderón-González, Amy Dumigan, Joana Sá‐Pessoa

et al.

PLoS Pathogens, Journal Year: 2024, Volume and Issue: 20(4), P. e1011900 - e1011900

Published: April 5, 2024

In vivo single-cell approaches have transformed our understanding of the immune populations in tissues. Mass cytometry (CyTOF), that combines resolution mass spectrometry with ability to conduct multiplexed measurements cell molecules at single resolution, has enabled resolve diversity subsets, and their heterogeneous functionality. Here we assess feasibility taking CyTOF one step further immuno profile cells while tracking interactions bacteria, a method term Bac-CyTOF. We focus on pathogen Klebsiella pneumoniae interrogating pneumonia mouse model. Using Bac-CyTOF, unveil atlas mice infected K . hypervirulent strain. The is characterized by decrease alveolar monocyte-derived macrophages. Conversely, neutrophils, inflammatory monocytes are an increase subpopulations expressing markers less active such as checkpoint PD-L1. These infected. show type VI secretion system (T6SS) contributes shape lung landscape. T6SS governs interaction monocytes/macrophages shifting from macrophages interstitial limiting infection monocytes. lack results cells, By probing , Acinetobacter baumannii strains limited survive vivo, uncover heightened recruitment relative high levels eosinophils characteristic subpopulation neutrophils features clearing infections. leverage Bac-CyTOF-generated knowledge platform investigate role DNA sensor STING sting -/- present consistent including reduced absence facilitates clearance.

Language: Английский

Citations

5
Klebsiella pneumoniae employs a type VI secretion system to overcome microbiota-mediated colonization resistance DOI Creative Commons
Andrew S. Bray, Christopher A. Broberg,

Andrew W. Hudson

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 22, 2025

Microbial species must compete for space and nutrients to persist in the gastrointestinal (GI) tract, our understanding of complex pathobiont-microbiota interactions is far from complete. Klebsiella pneumoniae, a problematic, often drug-resistant nosocomial pathogen, can colonize GI tract asymptomatically, serving as an infection reservoir. To provide insight on how K. pneumoniae interacts with resident gut microbiome, we conduct transposon mutagenesis screen using murine model colonization intact microbiota. Among genes identified were those encoding type VI secretion system (T6SS), which mediates contact-dependent killing gram-negative bacteria. From several approaches, demonstrate that T6SS critical colonization. Metagenomics vitro assays reveal reduces Betaproteobacteria T6SS-dependent manner, thus identifying specific targeted by pneumoniae. We further show gene expression controlled transcriptional regulators only occurs under conditions mimic environment. By enabling thrive gut, indirectly contributes pathogenic potential this organism. These observations advance molecular successfully colonizes tract. Through metagenomics, Bray et al. interacting microbiota partners revealed role its colonization, t6ss transcription occurring GI-mimetic conditions.

Language: Английский

Citations

0
Carbapenem-resistant Enterobacter hormaechei uses mucus metabolism to facilitate gastrointestinal colonization DOI Creative Commons
Ritam Sinha,

Elizabeth N. Ottosen,

Tshegofatso Ngwaga

et al.

mBio, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 29, 2025

ABSTRACT The emergence and global spread of carbapenem-resistant Enterobacter cloacae complex species present a pressing public health challenge. Carbapenem-resistant spp. cause wide variety infections, including septic shock fatalities in newborns immunocompromised adults. intestine may be major reservoir for these resistant strains, either by facilitating contamination fomites transfer to susceptible individuals, or through translocation from the gut bloodstream. For this reason, we sought establish neonatal mouse model investigate mechanisms underpinning colonization hormaechei . We describe new study spp., leading vital insights into adaptation E. environment during early stages intestinal colonization. observed successful proliferation 5-day-old infant gut, with primary localization colon following oral inoculation. also uncovered evidence that uses mucus as carbon source colon. Our findings underscore importance oxygen-dependent metabolic pathways, pyruvate dehydrogenase N- acetyl-D-glucosamine metabolism, proliferation, which aligns previous human studies. These are essential developing novel therapeutic strategies can serve decolonization therapies at-risk populations. IMPORTANCE Bloodstream infections caused pose significant clinical threat. acts site serves infection. To combat pathogen, it is crucial understand how colonize such knowledge pave way alternative targets. In study, developed gastrointestinal discovered plays key role Additionally, identified two catabolism pathways contribute This offers valuable host-pathogen interactions helps identify critical fitness factors , potentially guiding development vaccines minimize carriage patient populations at risk infection

Language: Английский

Citations

0
Klebsiella pneumoniae emerging anti-immunology paradigms: from stealth to evasion DOI Creative Commons
Joana Sá‐Pessoa, Ricardo Calderón-González,

Alix Lee

et al.

Trends in Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Klebsiella pneumoniae (KP) is a global threat to human health due the isolation of multidrug-resistant strains. Despite advancements in understanding KP's population structure, antibiotic resistance mechanisms, and transmission patterns, gap remains how KP evades defenses, allowing pathogen flourish tissues despite an activated immune system. infection biology has been shaped by notion that evolved shield from defenses more than actively suppress them. This review describes new paradigms exploits coevolution with innate system hijack effectors receptors ablate signaling pathways counteract cell-intrinsic immunity, making apparent can no longer be considered only as stealth pathogen.

Language: Английский

Citations

0
Whole -Genome sequencing and comparative genomics analysis of Klebsiella pneumoniae isolates from animal source foods DOI Creative Commons

Mufeeda Beegum,

P T Waseem Mirsab,

Bilal Ahmad Malla

et al.

The Microbe, Journal Year: 2025, Volume and Issue: unknown, P. 100274 - 100274

Published: Feb. 1, 2025

Language: Английский

Citations

0
Leveraging collateral sensitivity to counteract the evolution of bacteriophage resistance in bacteria DOI Creative Commons

YongQi Mu,

Yuqin Song, Xiaofei Tian

et al.

mLife, Journal Year: 2025, Volume and Issue: unknown

Published: March 18, 2025

The escalating antibiotic resistance crisis poses a major global health threat. Bacteriophage therapy offers promising alternative for combating multidrug-resistant infections. However, bacterial to phages remains significant hurdle. Innovative strategies are needed overcome this challenge. In study, we developed phage cocktail based on our library, consisting of three that suppressed carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKp). This capitalized dual instances collateral sensitivity, thereby constraining the evolution resistance. first-layered sensitivity arose from overlapping coverage between capsular polysaccharide (CPS) and lipopolysaccharide (LPS), rendering bacteria resistant CPS-binding but more susceptible LPS-binding phages. second-layered resulted an O serotype switch (from O1 O2), causing antigen-binding increasing susceptibility target O2 antigen. dual-layered effectively mitigated infection caused by CR-hvKp in mice. Our research highlights importance mechanism counteracting sophisticated strategy configuring cocktails eliminate

Language: Английский

Citations

0
Selenomethionine attenuates Klebsiella pneumoniae-induced jejunal injury in rabbits by inhibiting the TLR4/NF-κB pathway DOI
Lihui Shi, Yumei Liu, Jianing Wang

et al.

Microbial Pathogenesis, Journal Year: 2025, Volume and Issue: unknown, P. 107510 - 107510

Published: March 1, 2025

Language: Английский

Citations

0
Multidrug-resistant hypervirulent Klebsiella pneumoniae : an evolving superbug DOI
Yuzhong Zheng,

Xiaojue Zhu,

Chao Ding

et al.

Future Microbiology, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 13

Published: March 26, 2025

Multidrug-resistant hypervirulent Klebsiella pneumoniae (MDR-hvKP) combines high pathogenicity with multidrug resistance to become a new superbug. MDR-hvKP reports continue emerge, shattering the perception that K. (hvKP) strains are antibiotic sensitive. Patients infected have been reported in Asia, particularly China. Although hvKP can acquire drug genes, seems be more easily transformed from classical (cKP), which has strong gene uptake ability. To better understand biology of MDR-hvKP, this review discusses virulence factors, mechanisms, formation pathways, and identification MDR-hvKP. Given their destructive transmissible potential, continued surveillance these organisms enhanced control measures should prioritized.

Language: Английский

Citations

0